• Title/Summary/Keyword: $Lt_{50}$

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Toxicity Evaluation of Agricultural Insecticides on Workers of Pharaoh Ant, Monomorium pharaonis (Hyme-noptera : Formicidae) (애집개미 (Monomorium pharaonis) 일개미에 대한 농업용 살충제의 독성평가)

  • Kang, Shin-Ho;Han, Jong-Been;Park, Shin-Sub;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.10 no.3
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    • pp.218-224
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    • 2006
  • Toxicities of 34 agricultural insecticides was tested against Monomorium pharaonis workers by diet dipping method. Ten insecticides among them, bifenthrin, chlorpyrifos, chlorpyrifos-methyl, cypermethrin, dichlorvos, fenthion, fenitrothion, methidathion, pirimifos-methyl, and phenthoate showed 100% rapid mortality. $LC_{50}$ (ppm) values of selected insecticides were appeared on the order of pirimifos-methyl (0.33), chlorpyrifos-methyl (0.76), phenthoate (1.70), bifenthrin (1.78), dichlorvos (2.50), cypermethrin (9.92), chlorpyrifos (22.21), fenitrothion (36.58), fenthion (40.96), and methidathion (64.34). $LT_{50}$ (day) values by diet dipping method showed that dichlorvos, benfuracarb and cypermethrin acted more rapid than boric acid and hydramethylnon. The values of the former three were 0.25, 0.38 and 0.27 days, and those of the latter two were 3.4 and 2.6 days, respectively. In persistance effect tests, chlorpyrifos-methyl, fenthion and methidathion showed over 90% insecticidal activity for 13 days.

Determination of The Growth Performances and Meat Quality of Broilers Fed Saccharomyces cerevisiae as a Probiotic in Two Different Feeding Intervals

  • Macelline, W.H.D. Shemil Priyan;Cho, Hyun Min;Awanthika, H.K. Thilini;Wickramasuriya, Samiru Sudharaka;Jayasena, Dinesh. D.;Tharangani, R.M. Himali;Song, Zhang;Heo, Jung Min
    • Korean Journal of Poultry Science
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    • v.44 no.3
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    • pp.161-172
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    • 2017
  • This study was conducted to determine the effect of Saccharomyces cerevisiae (SC) as a dietary probiotic and evaluated the most suitable feeding interval for this probiotic on growth performance, carcass yield, and meat quality parameters in broiler chickens. In total, 1,050 one-day-old Cobb 500 chicks were randomly assigned to one of seven dietary treatment groups, in a $2{\times}3$ factorial arrangement with 3 SC dosages (0.6%, 1%, and 1.4%) and two feeding intervals [long term (LT) for 35 days and short term (ST) for 28 days after hatching], with a negative control diet (NC; 0% SC). Triplicate experiments were performed with 50 birds per cage. Broilers fed a diet including SC showed increased (p<0.01) daily gain and feed efficiency compared to the control. Further, broilers fed the 1.4% SC supplemented diet showed a significantly increased (p<0.01) average daily gain (ADG) and feed conversion ratio (FCR) compared to broilers fed the 0.6% and 1% SC incorporated diets. Similarly, broilers fed an LT SC diet showed a greater (p<0.01) increase in ADG and FCR compared to broilers fed an ST SC diet. Moreover, broilers fed an LT SC diet displayed a reduced (p<0.05) meat pH, gizzard weight, and increased (p<0.05) meat water-holding capacity compared to broilers fed an ST SC diet. Broilers fed the 1.4% SC supplemented diet showed increased (p<0.05) thigh muscle weight compared to broilers fed the 0.6% and 1% SC supplemented diets. In conclusion, broilers fed LT SC diets showed improved growth performance and carcass quality parameters compared to broilers fed ST SC diets, and the NC diet, from hatching to day 35.

Morphological, Phylogenetic and Biological Characteristics of Ectropis obliqua Single-Nucleocapsid Nucleopolyhedrovirus

  • Ma Xiu-cui;Xu Hai-Jun;Tang Mei-Jun;Xiao Qiang;Hong Jian;Zhang Chuan-Xi
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.77-82
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    • 2006
  • The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to $1.7\;{\mu}m$ in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rrl, polyhedrin, orfl629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration $(LC_{50}\;and\;LC_{90})$ and lethal time $(LT_{50}\;and\;LT({90})$ were conducted to test the susceptibility of E. obliqua larvae to the virus.

A Study on the Basic Shape of an MF Evaporator (MF증발기 기초 형상 설계에 관한 연구)

  • Park, Yong-Seok;Seong, Hong-Seok;Suh, Jeong-Se
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.18 no.6
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    • pp.39-44
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    • 2019
  • The evaporator is a key driver of an air conditioning system's efficiency. In this study, we study methods of maximizing the efficiency of a Massey Ferguson (MF) evaporator by measuring how the cooling performance of different shapes vary with temperature. We varied the tube insertion depth as well as the shape of the evaporator's header and tube. When we compare header shapes of "D", "Ellipse", and "Quadrangle" types, we find that the elliptical header creates the smallest pressure loss and the highest temperature difference. Between tube shapes of "Rectangular", "Projection", and "Circular" types, the "Projection" type tube creates the most temperature difference. We also investigated the depth of tube insertion in the header and find that tube insertion of 5 - 10 mm is feasible; we selected the depths of 5, 7, and 10 mm since they corresponded to approximately 30%, 50%, and 70% of the total width of the header. The tube insertion test demonstrated that a tube insertion depth of 7 mm creates the least pressure loss and the highest temperature difference. In conclusion, the optimal evaporator design uses an "Ellipse" type header, "Projection" type tube, and a tube insertion depth between 30 and 50% of the header width.

Effects of Acute Toxicity of Chemical Treatments on the Cultured Oliver Flounder, Paralichthys olivaceus (화학제 처리가 성장기 넙치 (Paralichthys olivaceus)에 미치는 급성독성 효과)

  • Ryu, Ho-Young;Bang, Jong-Deuk;Lee, Chu;Shim, Jeong-Min;Kim, Bong-Seok
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.223-230
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    • 1998
  • This study was carried out for the purpose of developing environmental friendly and effective chemical treatment method for the disease control in the land-based flounder culture which is industrially popular in the coastal area in Korean. The chemicals such as flounder, Paralichthys olivaceus and their effects on the fish based on the 24hr-$LC_{50}$, $LT_{50}$, 24-hour survival rate at each experimental concentration, recovery rate of the survived individual from chemical treatment, and the histological change of the gill after chemical treatment were investigated and analyzed. The 24hr-$LC_{50}$ was 321.65 ppm for formalin, 419.62 ppm for chlorine dioxide, and 395.97 ppm for hydrogen peroxide, respectively. The $LT_{50}$ was 15-hour for formalin, 17-hour for chlorine dioxide and 24-hour for hydrogen peroxide, respectively. Fishes exposed to the experimental concentration of three chemicals were quickly susceptible in the order of formalin, chlorine dioxide and hydrogen peroxide with a trend of shorter half lethal time at higher concentration. Initial survival rate of the flounder soon after chemical treatment was the highest in the hydrogen peroxide treatment compared with the other two chemicals. The histological damage by the hydrogen peroxide treatment was negligible compared with the other two chemicals. Accordingly, hydrogen peroxide treatment showed the lowest toxicity compared with the other two chemicals to the experimental fishes.

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Pathogenicity of Spodoptera exigua Nuclear Polyhedrosis Virus and Cross Infection of Baculoviruses to the Beet Armyworm, S. exigua (Lepidoptera: Noctuidae) (파밤나방 핵다각체병바이러스의 병원성과 곤충간상바이러스의 파밤나방에 대한 교차감염에 관한 연구)

  • 임대준;최귀문;강석권
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.212-218
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    • 1991
  • Pathogenicity of Spodoptera exigua nuclear polyhedrosis virus (SeMNPV) against the host insect and 8 species of lepidopterous insects and cross infection of baculoviruses to third instar of S. exigua larvae were studied to determine as a biocontrol agent for S. exigua. The median lethal concentrations($LC_{50}$)of the SeMNPV to egg mass was $2.855\times10^5$ PIBs/ml and higher than that to the larvae of S. exigua. Mortality of the SeMNPV in third ins tar larvae was more increased than that in first and fifth instar of S. exigua larvae by 1.16 and 4.11 times, respectively. The median lethal times($LT_{50}$) to $1.56\times10^6$ PIBs/ml was in the range of 4.25 to 5.04 days. Infectivity of the SeMNPV against eight species of lepidopterous insects was showed only in the host insect, S. exigua. Autographa cali/ornica MNPV, Mamestra barassicae MNPV, and Trichoplusia ni MNPV were cross-infected to third instar of S. exigua larvae among ten of baculoviruses tested.

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Electrical ppropperties of the In-situ Grown (Pb,La)$TiO_3$ Films on the Pt/MgO(100) (Pt/MgO(100) 기판상에 In-situ 성장된 (Pb,La)$TiO_3$ 박막의 전기적 특성)

  • 이상열;김민영;장호영;장지근
    • Proceedings of the Korean Vacuum Society Conference
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    • 1996.06a
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    • pp.180-181
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    • 1996
  • 적외선 센서의 재료로 활용되고 있는 ppLT 박막(두께:8000~9000$\AA$)을 ppt/MgO(100)의 하부 구조상에 50$0^{\circ}C$$600^{\circ}C$에서 스퍼터링 증착하여 결정성 및 전기적 특성을 조사하였다. $600^{\circ}C$로 in-situ 성장된 ppLT 박막은 c-축(00l) 방향으로 배향 성장되었 고, 비유전상수(r)와 유전정접(tan)의 값이 10kHz~100kHz의 주파수에서 $\varepsilon$r≒35와 tan$\delta$≒ 0.01로 나타났다. 잔류분극량(2ppr)과 초전계수(${\gamma}$)는 상온부근에서 2ppr≒5$\mu$C/cm2, ${\gamma}$≒4$\times$ 10-8C/cm2.$^{\circ}C$로 비교적 양호한 초전박막의 전기적 특성을 나타내었다.

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Determination of Lethal Concentrations and Lethal Times of Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens, to Control Green Peach Aphid, Myzus persicae (복숭아혹진딧물, Myzus persicae, 방제를 위한 제충국, 데리스, 고삼 추출물의 살충농도와 살충시간 결정)

  • Ka Hee Cho;Hyo Jung Kim;Song Hee Han;Young Cheol Kim
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.267-275
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    • 2023
  • Botanical extracts are employed in management of aphids. Extracts from Tanacetum cineariaiaefolium, Derris elliptica, and Sophora flavescens are widely used to control various insects. In this study, we determined concentrations of insecticidal active ingredients in commercial botanical extracts of these plants, and we investigated the time and concentration for lethal results with the green peach aphid, Myzus persicae. The concentrations of active ingredients, pyrethrins from T. cineariaiaefolium, rotenone from D. elliptica, and matrine and oxymatrine from S. flavescens, were determined after their fractionation by liquid chromatography followed by mass analysis and comparison with standard compounds. The extracts were tested for lethality in a bioassay with green peach aphids. Sprays at defined doses were applied to tobacco leaves infested with aphid nymphs. The lethal concentrations (LC50) were 20.4 ppm for pyrethrins, 34.1 ppm for rotenone, and 29.6 ppm for matrine at 48 h after treatments. At 100 ppm application levels, the lethal time LT50 was 13.4 h for pyrethrin, 15.1 h for rotenone, and 14.4 h for matrine. Kaplan-Meier analysis indicated the lethal times for the three botanical extracts at 100 ppm were significantly faster than application of a chemical insecticide, Sulfoxaflor, applied at the recommended level. These results provide baselines to develop and formulate single or mixed preparations containing botanical extracts to control green peach aphids on commercial crops.

The Rhizomes of Acorus gramineus and the Constituents Inhibit Allergic Response In vitro and In vivo

  • Lim, Hyun;Lee, Seung-Young;Lee, Kang-Ro;Kim, Yeong-Shik;Kim, Hyun-Pyo
    • Biomolecules & Therapeutics
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    • v.20 no.5
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    • pp.477-481
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    • 2012
  • The rhizomes of Acorus gramineus have frequently been used in traditional medicine mainly for sedation as well as enhancing brain function. In this study, the anti-allergic activity of A. gramineus was investigated. The 70% ethanol extract of the rhizomes of A. gramineus was found to inhibit the allergic response against 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat basophilic leukemia (RBL)-1 cells and ${\beta}$-hexosaminidase release from RBL-2H3 cells with $IC_{50}$'s of 48.9 and > $200{\mu}g/ml$, respectively. Among the 9 major constituents isolated, ${\beta}$-asarone, (2R,3R,4S,5S)-2,4-dimethyl-1,3-bis (2',4',5'-trimethoxyphenyl)tetrahydrofuran (AF) and 2,3-dihydro-4,5,7-trimethoxy-1-ethyl-2-methyl-3-(2,4,5-trimethoxyphenyl)indene (AI) strongly inhibited 5-LOX-catalyzed LT production in A23187-treated RBL-1 cells, AI being the most potent ($IC_{50}=6.7{\mu}M$). Against ${\beta}$-hexosaminidase release by antigen-stimulated RBL-2H3 cells, only AI exhibited strong inhibition ($IC_{50}=7.3{\mu}M$) while ${\beta}$-asarone and AF showed 26.0% and 39.9% inhibition at $50{\mu}M$, respectively. In addition, the ethanol extract of A. gramineus showed significant inhibitory action against the hapten-induced delayed hypersensitivity reaction in mice by oral administration at 200 mg/kg. Therefore, it is suggested that A. gramineus possesses anti-allergic activity and the constituents including ${\beta}$-asarone and AI certainly contribute to the anti-allergic activity of the rhizomes of A. gramineus.

A Cytoplasmic Polyhedrosis Virus Isolated from the Oriental Tobacco Budworm, Heliothis assulta Guenee (Lepidoptera: Noctuidae) (담배나방 세포질다각체병 바이러스의 동정 및 병원성에 관한 연구)

  • 임대준;장동숙;최귀문;강석권
    • Korean journal of applied entomology
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    • v.30 no.3
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    • pp.219-226
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    • 1991
  • A cytoplasmic polyhedrosis virus isolated from the oriental tobacco budworm, Heliothis assulta (HaCPV), was studied on morphology of the polyhedron and virus particles, analysis of viral protein and nucleic acid, and bioassay of the HaCPV to determine the feasibility of application as a microbial control agent. The shape of polyhedron was hexagonal ranging 0.5-3.7 ${\mu}m$ and the virus particles were icosahedral outline measured 55 nm in diameter. Polyhedral protein was composed of a major polypetide of 24.3 Kd and 5 minor components and virus particle had seven polypeptides ranging in 28.0 Kd-133. 6 Kd by the SDS-P AGE. The genome of virus was segmented with 10 double stranded RNA in the total mol. wt. of 18.08 Md ranging in 0.65 Md -2.79 Md. The $LC_{50}$ values of the HaCPV to the 3rd instar of H. assulta larvae were calculated to $2.895{\times}10^5PIBs/ml$. The $LT_{50}$ values in the concentration of $5.0{\times}10^{6}PIBs/ml$ was 16.4 days.

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