• Journal of Embryo Transfer
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• v.25 no.2
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• pp.103-110
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• 2010

The first morphogenetic event of preimplantation development, compaction, was required efficient production of porcine embryos in vitro. Compaction of the porcine embryo, which takes place at post 4-cell stage, is dependent upon the adhesion molecule E-cadherin. The E-cadherin through ${\beta}$-catenin contributes to stable cell-cell adhesion. Rho-associated kinase (ROCK) signaling was found to support the integrity of E-cadherin based cell contacts. In this study, we traced the effects of ROCK-1 on early embryonic development and structural integrity of blastocysts in pigs. Then, in order to gain new insights into the process of compaction, we also examined whether ROCK-1 signaling is involved in the regulation of the compaction mediated by E-cadherin of cellular adhesion molecules. As a result, real-time RT-PCR analysis showed that the expression of ROCK-1 mRNA was presented throughout porcine preimplantation stages, but not expressed as consistent levels. Thus, we investigated the blastocyst formation of porcine embryos treated with LPA and Y27632. Blastocysts formation and their qualities in LPA treated group increased significantly compared to those in the Y27632-treated group (p < 0.05). Then, to determine whether ROCK-1 associates embryonic compaction, we explored the effect of activator and/or inhibitor of ROCK-1 on compaction of embryos in pigs. The rate of compacted morula in LPA treated group was increased compared to that in the Y27632-treated group (39.7 vs 12.0%). Furthermore, we investigated the localization and expression pattern of E-cadherin at 4-cell stage porcine embryos in both LPA- and Y27632-treated groups by immunocytochemical analysis and Western blot analysis. The expression of E-cadherin was increased in LPA-treated group compared to that in the Y27632-treated group. The localization of E-cadherin in LPA-treated group was enriched in part of blastomere contacts compared to that Y27632-treated group. ROCK-1 as a crucial mediator of embryo compaction may plays an important role in regulating compaction through E-cadherin of the cell adhesion during the porcine preimplantation embryo. We concluded that ROCK-1 gene may affect the developmental potential of porcine blastocysts through regulating embryonic compaction.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.7
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• pp.839-846
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• 2007

In this paper, the first attempt to design a novel structure of dual-band feedforward linear power amplifier(FFW LPA) was presented. Up to now, primary technical difficulty has been the extension of the conventional signal canceller to the dual-band operation. Therefore, we propose the design technique of the dual-band equal group delayed carrier canceller, the dual-band equal group delayed intermodulation distortion(IMD) canceller and the dual-band FFW LPA. The operation frequency bands of the implemented dual-band FFW LPA are digital cellular($f_0=880$ MHz) and IMT-2000($f_0=2.14$ GHz) band, which are separated about 1.26 GHz. With the high power amplifier of 120 W PEP for commercial base-station application, IMD cancellation loop shows 20.45 dB and 25.04 dB loop suppression at each band of operation for 100 MHz. From the adjacent channel leakage ratio(ACLR) measurement with CDMA IS-95A 4FA and WCDMA 4FA signal, we obtained 16.52 dB improvement at the average output power of 41.5 dBm for digital cellular band, and 18.59 dB improvement at the average output power of 40 dBm for IMT-2000 band simultaneously.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.96-97
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• 2002

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (SIP) are bioactive lysophospholipids (LPs) that act as mediators in various cellular processes, such as cell growth, differentiation, survival, motility, and cytoskeletal reorganization (1,2). LPA and S1P are both abundant in serum and are produced by activated platelets and other cell types. (omitted)

• Journal of Chest Surgery
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• v.47 no.1
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• pp.32-34
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• 2014

Anomalous aortic origin of the left subclavian artery (LSCA) from the left pulmonary artery (LPA) is a rare congenital cardiac malformation. We describe a case of LSCA from the LPA via ductus arteriosus in association with a double-outlet right ventricle, which never has been reported previously in Korea.

• Biomedical Science Letters
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• v.12 no.1
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• pp.9-16
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• 2006

We investigated the effects of 835-MHz electromagnetic field (EMF), one of the most popular communication frequency band in Korean code-division multiple-access (CDMA) mobile phone system, on cellular signal transduction. For this, we examined the change of intracellular calcium $([Ca^{2+}]_i)$, reactive oxygen species (ROS) and F-actin polymerization after exposure to 835-MHz EMF followed by the treatment of agonists in Rat-2 fibroblast cells. Culture cells were pretreated with serum-tree medium and concomitantly exposed to 835-MHz at specific absorption rate (SAR) of 4.0 W/kg for 24 hr in a specialized designed apparatus based on Transverse Electro Magnetics (TEM) wave theory. Intracellular $Ca^{2+}$ responses to lysophosphatidic acid (LPA) and epidermal growth factor (EGF) in Rat-2 fibroblast after exposure to 835-MHz EMF were shown to be similar pattern as observed in normal cultured cells. However, the LPA-induced calcium spiking was slightly delayed to 7 sec and sustained thereafter to a little higher ground level under 835-MHz EMF radiation compared to unexposed cells. ROS production level by LPA in the exposed cells was not different from that in control. Furthermore, LPA induced the production of stress fibers with no significant difference in the exposed and unexposed cells. These results suggest that mobile phone radiation (835-MHz, SAR 4.0 W/kg) may not be directly related to signal transduction in Rat-2 fibroblasts except the slight effect of calcium spiking in LPA-induced cells but remain to be further elucidated for possible indirect intervention.

• Journal of Creative Information Culture
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• v.5 no.2
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• pp.117-134
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• 2019

The study is to compare the temperaments and characters of the gifted in invention with those of the genearl students, and also the characteristics of the subgroups of the gifted in invention through the LPA analysis. The results of the study are as follows: First, we compared the results of JTCI (Junior Temperament and Character Inventory) between the students entering Institute of Invention Science Education and the general students; through this comparison, we found out that the gifted in invention are more creative and willing to accept, generally feel more positive, and have the motivation to pursue meaningful goals and values above the personal needs than the non-gifted. Second, through the LPA analysis, the gifted in invention were divided into two subgroups; one group which showed far higher scores on pursuing stimulus, the subscale of temperaments, and slightly higher scores on autonomy, the subscale of characters, and the other group which showed the similar scores on pursuing stimulus and far higher scores on autonomy.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.8
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• pp.1407-1413
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• 2000

This paper presents a feedforward linear power amplifier (LPA) using error feedback technique to achieve low intermodulation distortions(IMD) of power amplifiers for base stations. Especially, the proposed linear power amplifier is applied to feedforward technique combined with error feedback technique, which has no loss of amplifier gain unlike typical feedback technique. The proposed LPA is designed by using HP ADS ver. 1.3, fabricated. When two-tone signals at 1850 MHz and 1851.25 MHz with -7 dBm/tone from synthesizers are injected into the main power amplifier with gain of 28 dB and P1dB of 1W, the proposed LPA could reduce more than 35 dB.

• Journal of Ginseng Research
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• v.45 no.3
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• pp.390-400
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• 2021

Background: We recently showed that gintonin, an active ginseng ingredient, exhibits antibrain neurodegenerative disease effects including multiple target mechanisms such as antioxidative stress and antiinflammation via the lysophosphatidic acid (LPA) receptors. Amyotrophic lateral sclerosis (ALS) is a spinal disease characterized by neurodegenerative changes in motor neurons with subsequent skeletal muscle paralysis and death. However, pathophysiological mechanisms of ALS are still elusive, and therapeutic drugs have not yet been developed. We investigate the putative alleviating effects of gintonin in ALS. Methods: The G93A-SOD1 transgenic mouse ALS model was used. Gintonin (50 or 100 mg/kg/day, p.o.) administration started from week seven. We performed histological analyses, immunoblot assays, and behavioral tests. Results: Gintonin extended mouse survival and relieved motor dysfunctions. Histological analyses of spinal cords revealed that gintonin increased the survival of motor neurons, expression of brain-derived neurotrophic factors, choline acetyltransferase, NeuN, and Nissl bodies compared with the vehicle control. Gintonin attenuated elevated spinal NAD(P) quinone oxidoreductase 1 expression and decreased oxidative stress-related ferritin, ionized calcium-binding adapter molecule 1-immunoreactive microglia, S100β-immunoreactive astrocyte, and Olig2-immunoreactive oligodendrocytes compared with the control vehicle. Interestingly, we found that the spinal LPA1 receptor level was decreased, whereas gintonin treatment restored decreased LPA1 receptor expression levels in the G93A-SOD1 transgenic mouse, thereby attenuating neurological symptoms and histological deficits. Conclusion: Gintonin-mediated symptomatic improvements of ALS might be associated with the attenuations of neuronal loss and oxidative stress via the spinal LPA1 receptor regulations. The present results suggest that the spinal LPA1 receptor is engaged in ALS, and gintonin may be useful for relieving ALS symptoms.

• Journal of Ginseng Research
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• v.45 no.3
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• pp.401-407
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• 2021

Background: Gintonin is an exogenous ginseng-derived G-protein-coupled lysophosphatidic acid (LPA) receptor ligand. LPA induces in vitro morphological changes and migration through neuronal LPA1 receptor. Recently, we reported that systemic administration of gintonin increases blood-brain barrier (BBB) permeability via the paracellular pathway and its binding to brain neurons. However, little is known about the influences of gintonin on in vivo neuron morphology and migration in the brain. Materials and methods: We examined the effects of gintonin on in vitro migration and morphology using primary hippocampal neural precursor cells (hNPC) and in vivo effects of gintonin on adult brain neurons using real time microscopic analysis and immunohistochemical analysis to observe the morphological and locational changes induced by gintonin treatment. Results: We found that treating hNPCs with gintonin induced morphological changes with a cell rounding following cell aggregation and return to individual neurons with time relapses. However, the in vitro effects of gintonin on hNPCs were blocked by the LPA1/3 receptor antagonist, Ki16425, and Rho kinase inhibitor, Y27632. We also examined the in vivo effects of gintonin on the morphological changes and migration of neurons in adult mouse brains using anti-NeuN and -neurofilament H antibodies. We found that acute intravenous administration of gintonin induced morphological and migrational changes in brain neurons. Gintonin induced some migrations of neurons with shortened neurofilament H in the cortex. The in vivo effects of gintonin were also blocked by Ki16425. Conclusion: The present report raises the possibility that gintonin could enter the brain and exert its influences on the migration and morphology of adult mouse brain neurons and possibly explains the therapeutic effects of neurological diseases behind the gintonin administration.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.20 no.1
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• pp.131-142
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• 2017

The function of urban parks as wildlife habitats is becoming increasingly important. The urban park serves as a key area for preserving urban biodiversity. The purpose of this study is to estimate the bird species diversity in 30 parks in Cheonan city and quantitatively analyze the influence of vegetation, park structure and landscape connectivity index. As the results, a total of 27 birds species and 1,509 individuals were found at the sites and the largest number of birds were found in the Cheongsa park with 17 species. The optimal regression model was selected as the explanatory variables for the logged park area (LPA), the tree cover ratio (Co_T) and the patch betweenness centrality (PB). LPA and Co_T mean the internal characteristics of the park, and PB was the external environmental variable meaning landscape connectivity. LPA was the most important factor (73.3%) as bird habitat, and the PB could be interpreted as a factor that should be considered as important (26.7%). It will be possible to consider these environmental variables in the park and green area construction and management.