• Journal of the Korean Mathematical Society
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• v.59 no.3
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• pp.519-548
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• 2022

In this paper, a stabilized-penalized collocated finite volume (SPCFV) scheme is developed and studied for the stationary generalized Navier-Stokes equations with mixed Dirichlet-traction boundary conditions modelling an incompressible biological fluid flow. This method is based on the lowest order approximation (piecewise constants) for both velocity and pressure unknowns. The stabilization-penalization is performed by adding discrete pressure terms to the approximate formulation. These simultaneously involve discrete jump pressures through the interior volume-boundaries and discrete pressures of volumes on the domain boundary. Stability, existence and uniqueness of discrete solutions are established. Moreover, a convergence analysis of the nonlinear solver is also provided. Numerical results from model tests are performed to demonstrate the stability, optimal convergence in the usual L² and discrete H¹ norms as well as robustness of the proposed scheme with respect to the choice of the given traction vector.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.27 no.9
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• pp.594-599
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• 2014

The heat dissipation conditions of high-power 5 watt LEDs-based searchlight modules were optimized with varying LED bar'shape, materials, and ambient temperature. The LED junction temperature was estimated by using Computational Fluid Dynamics simulation. The optimal heat dissipation conditions were found as follows; LED bar' shape: L=80 mm, W=4 mm, t=10 mm, copper material, LED junction temperature of $116.6^{\circ}C$, ambient temperature of $50^{\circ}C$, total mass of 184 g, and shadowing area of $320mm^2$. The difference between the junction temperatures of our fabricated and simulated LEDs-based searchlight modules is about $3^{\circ}C$, which confirms the validity of our thermal simulation results.

• The Journal of the Convergence on Culture Technology
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• v.10 no.4
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• pp.41-46
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• 2024

In this study, we sought to confirm the potential of the Chaetomorpha torta extract as a raw material for cosmetics. Accordingly, we sought to find the optimal extraction conditions for Chaetomorpha torta by ethanol concentration and to check whether it has an anti-inflammatory effect by confirming the inhibitory effect of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). The main contents of the experiment results are as follows. First, when DPPH radical scavenging ability and polyphenol yield were used as the criteria for optimal extraction conditions of Chaetomorpha torta, 70% concentration ethanol extract was most suitable. Second, as a result of cytotoxicity evaluation using Raw 264.7, no cytotoxicity was observed at concentrations below 100 ㎍/mL. Third, as a result of measuring the inhibitory effect of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in LPS-induced Raw 264.7 cells, the production of all cytokines decreased in proportion to the concentration, and 100 ㎍/mL It was confirmed that the concentrations were 73.76±2.6%, 84.8±2.42%, and 91.91±0.47%, respectively, showing excellent anti-inflammatory properties. According to these research results, it appears that the extract of the Chaetomorpha torta can be valuable as a raw material for cosmetics with anti-inflammatory properties, and if research on antioxidant, antibacterial, and anti-inflammatory activities related to green algae is conducted more actively, marine resources can be used as useful basic data. It is believed that it will be.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.13 no.4
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• pp.281-292
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• 2009

The solution of the interface problem or Poisson problem with concave corner has singular perturbation at the interface corners or singular corners. The decoupled dual singular function method (DDSFM) which exploits the singular representations of the solutions was suggested in [3, 9] and estimated optimal accuracy in [10]. The convergence rates consist with theoretical results even for the problems with very strong singularity, with the efficiency depending on parameters used in the methods. Furthermore the errors in $L^2$ and $L^\infty$-spaces display some oscillation, in the cases with meshsize not small enough. In this paper, we present an answer to remove the oscillation via numerical experiments. We observe the effects of parameters in DDSFM, and show the consisting efficiency of the method over the strong singularity.

• Proceedings of the Safety Management and Science Conference
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• 2002.11a
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• pp.273-277
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• 2002

In the electrode fabrication of unit cell, we found that optimal the electrochemical characteristics were obtained with at 90 wt.% of activated carbon(BP-20), 5 wt.% of conducting agent(Ppy, Super P) and 5 wt.% of P(VdF-co-HFP)/PVP mixed binder. The electrochemical characteristics of unit cell with Ppy improver were as follows : 37.6 F/g of specific capacitance, 0.98 $\Omega$ of AC-ESR, 2.92 Wh/kg and 6.05 Wh/L of energy density, and 754 W/kg and 1,562 W/L of power density. It was confirmed that internal resistance were reduced due to the increase of electrical conductivity and filling density by the introduction of conductivity agent, and content of conducting agent was suitable in the range of 4~6 wt.%. According to the impedance measurement of the electrode with conductivity agent, we found that it was possible to charge rapidly by the fast steady-state current convergence due to low equivalent series resistance(AC-ESR), fast charge transfer rate at interface between electrode and electrolyte, and low RC time constant.

• Journal of the Korea Convergence Society
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• v.8 no.12
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• pp.199-208
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• 2017

This study investigated the effects of overtime work on the health-related quality of life (HRQoL) of Korean blue-collar workers. This cross-sectional study collected data on 229 Korean blue-collar workers in six small-sized companies from October to November 2015. The data were analyzed using hierarchical multiple regression analysis to estimate the effect of overtime work while considering convergence variables. In the hierarchical regression model, when overtime work variable was included in the model, $R^2$ change was statistically significant. The significant predictors for HRQoL were overtime work (${\beta}=.152$, p=.025), depression (${\beta}=-.192$, p=.003) and night shift work (${\beta}=-.201$, p=.032). The results of this study provide a basic data for establishing optimal working hours standards for improving the quality of life of Korean blue-collar workers.

• Composites Research
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• v.33 no.1
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• pp.13-18
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• 2020

Recently, the fire rescue truck in problem proceed research it for the fast works action and for pass the small road. So we were research for weight reduction. In this study, the (NO. 5) fifth boom of the fire rescue truck have 288 mm(W) × 299 mm(D) × 3,691 mm(L) with a maximum load of 876 kg and the thickness of 3 mm of the Steel Boom. This changing of Steel (STRENX960) to CFRP was weave Carbon Fiber NCF (±45°, 2axis) and then it make the NCF Prepreg. This process was designed based on structural analysis, the effects of NCF Prepreg (±45°) on torsion were identified, and the optimal design was made with Stacking Pattern (b). Stack patterns were optimized for levels equal or higher than existing Steel Boom and CFRP Boom stacked in the UD direction, and finally, the lightening effect on weight of approximately 49.6% of the steel was identified.

• The Korean Journal of Pain
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• v.34 no.1
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• pp.19-26
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• 2021

Background: Prolotherapy is a proliferation therapy as an alternative medicine. A combination of dextrose solution and lidocaine is usually used in prolotherapy. The concentrations of dextrose and lidocaine used in the clinical field are very high (dextrose 10%-25%, lidocaine 0.075%-1%). Several studies show about 1% dextrose and more than 0.2% lidocaine induced cell death in various cell types. We investigated the effects of low concentrations of dextrose and lidocaine in fibroblasts and suggest the optimal range of concentrations of dextrose and lidocaine in prolotherapy. Methods: Various concentrations of dextrose and lidocaine were treated in NIH-3T3. Viability was examined with trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Migration assay was performed for measuring the motile activity. Extracellular signal-regulated kinase (Erk) activation and protein expression of collagen I and α-smooth muscle actin (α-SMA) were determined with western blot analysis. Results: The cell viability was decreased in concentrations of more than 5% dextrose and 0.1% lidocaine. However, in the concentrations 1% dextrose (D1) and 0.01% lidocaine (L0.01), fibroblasts proliferated mildly. The ability of migration in fibroblast was increased in the D1, L0.01, and D1 + L0.01 groups sequentially. D1 and L0.01 increased Erk activation and the expression of collagen I and α-SMA and D1 + L0.01 further increased. The inhibition of Erk activation suppressed fibroblast proliferation and the synthesis of collagen I. Conclusions: D1, L0.01, and the combination of D1 and L0.01 induced fibroblast proliferation and increased collagen I synthesis via Erk activation.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.607-615
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• 2018

Objective: This study was conducted to isolate the cellulolytic microorganism from the rumen of Holstein steers and characterize endoglucanase gene (Cel5A) from the isolated microorganism. Methods: To isolate anaerobic microbes having endoglucanase, rumen fluid was obtained from Holstein steers fed roughage diet. The isolated anaerobic bacteria had 98% similarity with Eubacterium cellulosolvens (E. cellulosolvens) Ce2 (Accession number: AB163733). The Cel5A from isolated E. cellulolsovens sp. was cloned using the published genome sequence and expressed through the Escherichia coli BL21. Results: The maximum activity of recombinant Cel5A (rCel5A) was observed at $50^{\circ}C$ and pH 4.0. The enzyme was constant at the temperature range of $20^{\circ}C$ to $40^{\circ}C$ but also, at the pH range of 3 to 9. The metal ions including $Ca^{2+}$, $K^+$, $Ni^{2+}$,$Mg^{2+}$, and $Fe^{2+}$ increased the endoglucanase activity but the addition of $Mn^{2+}$, $Cu^{2+}$, and $Zn^{2+}$ decreased. The Km and Vmax value of rCel5A were 14.05 mg/mL and $45.66{\mu}mol/min/mg$. Turnover number, Kcat and catalytic efficiency, Kcat/Km values of rCel5A was $96.69(s^{-1})$ and 6.88 (mL/mg/s), respectively. Conclusion: Our results indicated that rCel5A of E. cellulosolvens isolated from Holstein steers had a broad pH range with high stability under various conditions, which might be one of the beneficial characteristics of this enzyme for possible industrial application.

• ALGAE
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• v.39 no.2
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• pp.109-127
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• 2024

High production and efficient harvesting of microalgae containing high omega-3 levels are critical concerns for industrial use. Aeration can elevate production of some microalgae by providing CO₂ and O₂. However, it may lower the production of others by generating shear stress, causing severe cell damage. The mixotrophic dinoflagellate Gymnodinium smaydae is a new, promising microalga for omega-3 fatty acid production owing to its high docosahexaenoic acid content, and determining optimal conditions and methods for high omega-3 fatty acid production and efficient harvest using G. smaydae is crucial for its commercial utilization. Therefore, to determine whether continuous aeration is required, we measured densities of G. smaydae and the dinoflagellate prey Heterocapsa rotundata in a 100-L semi-continuous cultivation system under no aeration and continuous aeration conditions daily for 9 days. Furthermore, to determine the optimal conditions for harvesting through centrifugation, different rotational speeds of the continuous centrifuge and different flow rates of the pump injecting G. smaydae + H. rotundata cells into the centrifuge were tested. Under continuous aeration, G. smaydae production gradually decreased; however, without aeration, the production remained stable. Harvesting efficiency and the dry weights of omega-3 fatty acids of G. smaydae + H. rotundata cells at a rotational speed of 16,000 rpm were significantly higher than those at 2,000-8,000 rpm. However, these parameters did not significantly differ at injection pump flow rates of 1.0-4.0 L min^-1. The results of the present study provide a basis for optimized production and harvest conditions for G. smaydae and other microalgae.