• 치과방사선
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• 제12권1호
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• pp.63-67
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• 1982

The author modified the Aquard 100 head holder device from Denar Co. to be adequate to the Koreans and to enhance the standardization and reproducibility of the temporomandibular joint radiographs. The results were obtained as follows; 1. The projected central ray was at an angle of 25 degrees. 2. Standardization of patients head position was obtained by means of vertical and horizontal bar which were attached to the head holder. 3. Arbitrary 3 points (A.B.C.) which were on the TMJ area of dry skull were measured for the identification of reproducibility. 4. Measured distances in dry skull were as follow; A-B, 28.0±0.25, B-C 11.9±0.25 and C-A 30.4±0.21, in closed position.

• 생약학회지
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• 제39권4호
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• pp.316-323
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• 2008

In this study, we have investigated the HPLC analysis methods and quantitative analysis of standard compounds for quality standardization of a medicinal crude drug GCSB-5, a herbal formulation consisting of 6 medicinal plants (Acanthopanacis Cortex, Achyranthis Radix, Ledebouriellae Radix, Cibotii Rhizoma, Glycine Semen, Eucommiae Cortex) which are used in traditional medicine to treat various bone disorders. HPLC analysis methods of acanthoside D(Acanthopanacis Cortex), 20-hydroxyecdysone(Achyranthis Radix) which were known standard compounds among 6 medicinal plants were developed on crude material and product. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. Content of acanthoside D and 20-hydroxyecdysone on raw material of GCSB-5 were decided at 0.577-0.578 mg/g and 0.311-0.312 mg/g. And we confirmed that content of acanthoside D and 20-hydroxyecdysone on GCSB-5 preparation were 0.302-0.303 mg/capsule and 0.113-0.115 mg/capsule.

• 생약학회지
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• 제40권1호
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• pp.18-24
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• 2009

GCSB-5 preparation is a purified extract from a mixture six herbal medicines (Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used in traditional medicine to treat various bone disorders. This study was carried out to obtain the HPLC analysis method that can be used to establish quantitative analysis of Glycine Semen Nigra and Eucommiae Cortex for standardization of GCSB-5 preparation. HPLC analysis methods for the simultaneous determination of genistin (Glycine Semen Nigra) and geniposide (Eucommiae Cortex) were established for the quality control of herbal medicinal raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. As the result of quantitative analysis, the contents of genistin and geniposide in the raw material of GCSB-5 preparation were 0.0426-0.0427 mg/g and 0.431-0.432 mg/g. And GCSB-5 preparation contained genistin of 0.0202-0.0203 mg/capsule and geniposide of 0.211-0.212 mg/capsule, respectively.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2335-2340
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• 2014

Paclitaxel is hydrophobic in nature and is recognized as a highly toxic anticancer drug, showing adverse effects in normal body sites. In this study, we developed a polymeric nano drug carrier for safe delivery of the paclitaxel to the cancer that releases the drug in a sustained manner and reduces side effects. N-isopropylacrylamide/vinyl pyrrolidone (NIPAAm/VP) nanoparticles were synthesized by radical polymerization. Physicochemical characterization of the polymeric nanoparticles was conducted using dynamic light scattering, transmission electron microscopy, scanning electron microscopy and nuclear magnetic resonance, which confirmedpolymerization of formulated nanoparticles. Drug release was assessed using a spectrophotometer and cell viability assays were carried out on the MCF-7 breast cancer and B16F0 skin cancer cell lines. NIPAAm/VP nanoparticles demonstrated a size distribution in the 65-108 nm range and surface charge measured -15.4 mV. SEM showed the nanoparticles to be spherical in shape with a slow drug release of ~70% in PBS at $38^{\circ}C$ over 96 h. Drug loaded nanoparticles were associated with increased viability of MCF-7 and B16F0 cells in comparison to free paclitaxel. Nano loaded paclitaxel shows high therapeutic efficiency by sustained release action for the longer period of time, i increasing its efficacy and biocompatibility for human cancer therapy. Therefore, paclitaxel loaded (NIPAAm/VP) nanoparticles may provide opportunities to expand delivery of the drug for clinical selection.

• 한국식품과학회지
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• 제35권2호
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• pp.316-319
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• 2003

서울 등의 재래시장 및 대형 마켓 등지에서 판매되고 있는 상품김치 36종을 수집하여 pH, 산도, 색도 및 매운맛 특성을 조사하였다. 그 결과 숙성 정도가 pH $3.88{\sim}6.20$, 총산도 $0.28{\sim}1.13%$로 제조 즉시부터 어느 정도 과숙된 김치까지 다양하였다. 김치의 매운맛과 색도에 영향을 주는 요인을 고춧가루로 보고 고추의 매운맛 성분인 capsaicinoids 함량을 측정한 결과 $018{\sim}2.02\;mg%$를 나타내었고, ASTA값은 수분 함량을 고려하여 환산한 결과 가장 낮은 시료는 0.52, 가장 높은 시료는 4.1이었다. 즉 상품김치의 경우 규격화되어 있지 않고, 국내시장에서 발효단계, 매운맛 및 붉은색 정도가 다양하게 판매되고 있었다.

• 분석과학
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• 제25권6호
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• pp.402-409
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• 2012

연속흐름 수소화물 생성-유도결합 플라스마 원자 방출 분광기(FI-HG-ICP-AES) 이용하여 플라스틱 소재에 함유된 비소를 정량하기 위한 조건을 얻었다. 플라스틱 시료를 습식 산 분해 방법 및 마이크로파 산 분해 방법으로 전처리하여 시험 용액을 조제하였다. 인증표준물질(CRM)인 CRM-EC680k 및 CRM-EC681k를 사용하여 측정한 값과 인증값을 비교하여 분석 방법의 재현성 및 정확성을 확인하였다. 습식 산 분해 방법 및 마이크로파 산 분해 방법으로 전처리하여 FI-HG-ICP-AES로 측정한 결과 CRM-EC680k의 경우에는 4.1~4.3 mg/kg (인증값 $4.1{\pm}0.5$ mg/kg)이고, CRM-EC681k의 경우에는 28.9~30.6 mg/kg (인증값 $29.1{\pm}1.8$ mg/kg)이었다.

• Journal of Astronomy and Space Sciences
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• 제25권2호
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• pp.87-100
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• 2008

소백산 천문대의 61cm 망원경에 부착된 bvri CCD 측광 시스템으로 근접쌍성 V523 Cas를 2003년 1월에 4일 밤을 관측하여 792점의 CCD 영상을 얻었다. 이 영상 내에 있는 17개의 BVRI 표준성을 이용하여 소백산 천문대의 bvri 측광계와 Johnson-Cousins의 BVRI 측광계 사이의 변환 관계를 V=v-0.0689(B-V)+0.0063, B-V=1.3197(b-v)-0.1733, V-R=0.9210(v-r)-0.1309, R-I=0.8892(r-i)-0.1055와 같이 구하였다. 이 식을 이용하여 근접쌍성 V523 Cas 근처에 있는 57개 별들의 표준 V등급과 색지수(B-V, V-R, R-I)를 결정하였다.

• 대한한방내과학회지
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• 제33권1호
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• pp.1-13
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• 2012

Background : In oriental medicine, the status of the tongue is an important indicator to diagnose the condition of one's health, such as physiological and the clinicopathological changes of internal organs. A tongue diagnosis is not only convenient but also non-invasive, and therefore widely used in Oriental medicine. However, the tongue diagnosis is greatly affected by examination circumstances, patient's posture, and doctor's diagnosis criteria. Objectives : This study was designed to assure the necessity for standardization of tongue diagnosis based on diagnostic criteria of tongue coating thickness (TCT). Methods : Thirty tongue photographs were acquired and analyzed by digital tongue diagnosis system (DTDS) which measured the percentage of TCT on the tongue surface. Fifteen oriental medical doctors evaluated TCT in 30 photographs. Afterward, the 15 assessors were trained for diagnostic criteria of TCT and evaluated the photographs again. The intraclass correlation coefficient (ICC) was used to obtain the agreement rate among the 15 assessors and the agreement rate between assessors' TCT scores and DTDS values. Results : The agreement rate among the 15 assessors after training was higher than before training. The agreement rate between assessors' TCT scores and DTDS values after training was also higher than before training. Furthermore, the difference of the agreement rate between before and after training was significant (p<0.05). Conclusions : The standardization of diagnostic criteria of TCT increased the agreement rate among the assessors and the agreement rate between assessors' TCT scores and DTDS values. Therefore, the standardization of diagnostic criteria is expected to contribute to the objectification and quantification of the tongue diagnosis system.

• 한국보육지원학회지
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• 제12권4호
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• pp.111-130
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• 2016

This study examined the reliability and validity evidence of the Korean version of Wechsler Primary & Preschool Scale for Intelligence(K-WPPSI-IV), which was standardized recently. Following the stratified sampling for Korean population in terms of region and maternal education level, 1,700 children aged 2.6~7.7 years were recruited for Korean norm development. The split-half reliability (N=1,700) was high(Fisher's z(.75-.89)) and test-retest reliability(N=100) was satisfactory ranging from .95 to .68. The inter-rater reliability(N=110) was very high(.96-1.0). Discriminant and convergent validities were revealed by correlational patterns among subtests and indices. Future studies are needed to extend both reliability and validity of K-WPPSI-IV by collecting data with a various sample group and a variety of instruments.

• 생약학회지
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• 제31권4호
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• pp.416-420
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• 2000

Ursolic acid was isolated from Prunellae Herba (Prunella vulgaris var. lilacina) and identified by direct comparison with an authentic sample. A method of analysis for the evaluation of ursolic acid was developed based on extraction of ground plant material, followed by quantitative determination using capillary gas chromatography of the TMS derivative. Quantitative analysis by GC after derivatisation under mild silylating conditions showed 0.31% ursolic acid in 20 samples collected throughout regions of Korea while no ursolic acid was detected in the samples of the whole plant of Thesium chinense, a substitute for Prunellae Herba in southern regions of Korean peninsula.