• Journal of Applied Biological Chemistry
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• 제58권2호
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• pp.139-143
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• 2015

본 연구는 다양한 효능을 지닌 초과(Amomum tsao-ko Crevost et Lemaire)의 안전한 이용을 위한 독성평가로 식품의약품안전처 고시 제2014-6호 '의약품등의 독성시험기준'에 맞는 독성시험법에 따라 Balb/c mouse를 이용하여 3주간 반복경구투여를 통해 초과의 안전성을 확인하고자 하였다. 3주간 반복 경구투여 후 체중, 장기중량 측정, 혈액분석 및 혈액생화학 검사를 실시하여 안전성을 확인 한 결과, 초과에 의한 특별한 증상이나 체중, 장기중량의 변화는 관찰되지 않았으며, 복대동맥으로부터 채혈한 혈액을 통한 혈구분석결과에서도 대조군과 초과 추출물 투여군 간의 통계적인 유의성을 관찰 할 수 없었다. 또한 혈청을 이용하여 간기능(GOT, GPT, LDH, ALB, TP-S, T-BIL, D-BIL), 신장기능(BUN, CRE), 지질영양 관련(TG), 전해질 관련(I.P) 지표들의 생화학분석을 수행한 결과, 대조군과 유사하게 모두 정상 범위 내의 결과를 나타내었다. 이러한 결과를 통하여 초과 추출물의 최대무독성용량은 최고 투여량인 2000 mg/kg 이상으로 판단되며, 본 연구결과는 초과의 기능성 식품, 화장품, 의약품 등 다양한 소재로서의 활용에 안전성 관련 기초자료로 이용될 수 있을 것으로 사료된다.

• 한국가금학회지
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• 제41권2호
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• pp.87-92
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• 2014

The avian embryos have been used as a good model to study embryonic development. Due to its unique development in the eggshell, avian embryos can be cultured and hatch in the surrogate eggshell system. In this study, we examined the viability, normal development and hatchability of Korean Oge (KO) chicken embryos in White Leghorn (WL) surrogate eggshells. Donor KO embryos at 3-day and 4-day-old were transferred into recipient WL eggshells, incubated for further 18 days at $37.5^{\circ}C$ with 70% of humidity until hatching. The viability of 3-day-old KO embryos at 7, 14 and 21 day in surrogate eggshell were 70.0%, 43.8% and 23.1%, respectively. In contrast, the viability of 4-day-old KO embryos at 7, 14 and 21 day in surrogate eggshells were 87.1%, 55.6% and 36.0%, respectively. The hatchability of KO embryos transferred into surrogate eggshells at 3-day-old was 23.1%, whereas embryos transferred at 4-day-old was 36.0%. Furthermore, the development of all viable embryos from 3-day group and 4-day group were normal. Our results suggested that culture of KO embryos in WL surrogate eggshells is highly possible, and transfer of donor embryos at 4-day-old may yield higher percentage of hatchability. This study may provide potential knowledge for the conservation of wild and endangered birds through surrogate system.

• Applied Biological Chemistry
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• 제13권1호
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• pp.59-64
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• 1970

Bence Jones 단백질(蛋白質)(As,Ik,In)를 DEAE-Sephadex A-50 Column으로 0.02M phosphate Buffer(pH8.)와 0.5M NaCl를 gradient로 사용(使用)해 정재(精製)하였다. 시료(試料) As(K-형(型))의 정재결과(精製結果) 주성분(主成分)인 F-1는 조단백질(粗蛋白質) 500mg로부터 350mg 얻었으며 다른 시료(試料) Im와 Ik($lambda$-형(型))에서도 각각 주성분(主成分)을 242mg(Im)와 146mg(Ik) 얻을 수 있었다. 정재(精製)한 시료(試料)는 그의 순도(純度)를 알기 위해 Poly acryl amide의 Disc electrophoresis로 확인한 후 K-형(型) Bence jones단백질(蛋白質)의 일종(一種)인 As와 $lambda$-형(型)의 일종(一種)인 IM를 6N-HCI로 $105^{\circ}C$에서 20시간(時間) 가수분해(加水分努)후 Amino 산(酸) 조정(組成)을 살펴보았다. DNP법(法)으로 시료(試料) K-형(型)(As, Ko, Ta)의 단백질(蛋白質)의 N-말단(末端)을 검출(檢出)해 Ta는 glutamic acid, Ko, As는 Aspartic acid임을 확인하였으며 Paper 상(上)에 나타난 DNP-Amino 산(酸)은 5% $NaHCO_3$ (pH8) 4ml에 추출(抽出)해 그 수량(收量)을 ${\varepsilon}=18.1{\times}10^3DNP$ $Asp\;{\varepsilon}=17.41{\times}10^(3){\;}DNP{\;}Glu$에 의(依)해 산출(算出)하였더니 Ko는 54.3%, As는 65%이였으며 Ta는 85%의 수량(收量)이였다. ${\lambda}$-형(型)의 Im,Ik의 시료(試料)도 DNP법(法)으로 N-말단(末端)을 검출(檢出)해 보았더니 검출(檢出)되지 아니하였다.

• 대한본초학회지
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• 제31권2호
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• pp.13-19
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• 2016

Objectives : The present study was designed to evaluate the protective effect of Allium tuberosum (AT) extract on atherosclerosis in LDL receptor knockout (LDLr KO) mouse fed western diet.Methods : The AT was extracted 70% ethanol. The experimental groups were divided with four groups of LDLr KO mice, one group fed a normal diet and the others fed a Western diets for 8weeks. Two Western diet groups were orally administered AT extract at dosage of 100 and 300 mg/kg body weight. The body weight and food intake were measured every day. We measured levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and Glucose in serum. Also, effect of AT extract performed using H&E staining.Results : The AT treatment groups showed decrease in body weight and food efficiency in comparison with control group. Blood biochemistry parameters such as TG, TC, LDL, and glucose levels were increased in control group, while AT treatment groups were reduced. Also, the increased levels of ALT and AST were improved by AT extract. We confirmed that the weights of liver, kidney, subcutaneous fat, epididymal fat, kidney leaf fat, and intraabdominal fat were change in LDLr KO mice treated AT extract. In addition, histopathological changes in liver and aorta were similar to normal group.Conclusions : Based on these results, the AT extract is considered to make prevention of atherosclerosis through reduction and functional improvement of the liver and vascular endothelial cells in the body fat accumulation and lipid content in LDLr ko mouse model.

• BMB Reports
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• 제38권1호
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• pp.111-114
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• 2005

Heat shock proteins (HSPs) are known to protect cells from oxidative stress and other types of injuries. We previously reported the neuroprotective effect of HSP70 following cerebral ischemia and reperfusion using hsp 70.1 knockout (KO) mice. However, the precise role of HSP70 in neuroprotection has not been established yet. The purpose of this study was to investigate the relationship between HSP70 and antioxidant enzymes using hsp 70.1 KO mice. The activities of both SOD-1 and SOD-2 were significantly decreased in hsp 70.1 KO mice than in the wild type (WT) littermates. SOD-1 protein level in the hsp 70.1 KO mice was lower than that of WT. We speculate that HSP70 might be involved in regulation of expression of SOD-1 at the level of transcription or by post-transcriptional modification.

• 한국대기환경학회지
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• 제20권4호
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• pp.539-553
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• 2004

Up to the present time, many methods to estimate emissions from a particular diesel engines have wholly depended on the quantity of diesel fuel consumed. Then, the recommended emission factors were normalized by fuel consumption, and further total activity was estimated by the total fuel consumed. One of main purposes in the study is newly to develop emission factors for the railroad diesel rolling stock (RDRS) and to estimate a total amount of major gaseous pollutants from the RDRS in Korea. Prior to develop a Korean mode emission factor. the emission factor from the USEPA was simply applied for comparative studies. When applying the USEPA emission factors, total exhaust emissions from the RDRS in Korea were estimated by 28,117tons of NOx, 2,832.3tons of CO, and 1,237.5tons of HC, etc in 2001. In this study, a emission factor for the RDRS, so called the KoRail mode (the Korean Railroad mode) has been developed on the basis of analyzing the driving pattern of the Gyeongbu-Line especially for the line-haul mode. Explicitly to make the site specific emission factors, many uncertainty problems concerning weighting factors for each power mode, limited emission test, incomplete data for RDRS, and other important input parameters were extensively examined. Total exhaust emissions by KoRail mode in Korea were estimated by 10,960tons of NOx, and 4,622tons of CO, and so on in the year of 2001. The emissions estimated by the USEPA mode were 2.6 times higher for NOx, and 1.6 times lower for CO than those by the KoRail mode. As a conclusion, based on the emission calculated from both the USEPA mode and the KoRail mode, the RDRS is considered as one of the significant mobile sources for major gaseous pollutants and thus management plans an(1 control strategies for the RDRS must be established to improve air quality near future in Korea.

• The Plant Pathology Journal
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• 제16권2호
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• pp.110-117
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• 2000

Potato virus X (PVX-KO) showing mild mosaic and stunting symptoms on potato (Solanum tuberosum) in Kangwon area has been isolated and characterized. EM observation of the purified virus particles showed flexuous rod shape of about 520 nm in length. The coat protein (CP) of the virus had a molecular weight of 31 kDa in SDS-PAGE analysis, and the viral RNA was approximately 6.4 kb in size in denatured agarose gel electro-phoresis. In gel-immunodiffusion tests, it reacted strongly with an antiserum to common PVX from BIOREABAAG (USA). A rabbit antiserum was produced using purified virus and used for routine PVX detection by ELISA. Cultivated potatoes in Kangwon and other areas were frequently infected with PVX-KO. Both Datura stramonium and Nicotiana tabaccum cultivars developed necrotic local lesions 5 days after inoculation, and systemic mosaic symptoms with vein clearing 2 weeks after inoculation. All the features agree with the description of other PVX strains. To confirm and determine PVX strains, reverse transcription-polymerase chain reaction experiment was conducted using specific primers for viral CP. Amplified DNA fragments were cloned and sequenced. Results showed nucleotide sequence homologies of about 88 to 99% to other PVX strains. Based on CP amino acid sequence deduced from nucleotide sequences and host range studies PVX-KO is considered a member of the type X subgroup of PVX.

• 한국컴퓨터정보학회논문지
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• 제26권12호
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• pp.1-9
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• 2021

재난문자는 재난 발생 시 국가에서 해당 지역에 있는 시민들에게 보내는 문자 메시지다. 재난문자의 발송 건수는 점점 증가하여, 불필요한 재난문자가 많이 수신됨에 따라 재난문자를 차단하는 사람들이 증가하고 있다. 이와 같은 문제를 해결하기 위하여, 본 연구에서는 재난문자를 재난 유형별로 자동으로 분류하고 수신자에 따라 필요한 재난의 재난문자만 수신하게 하는 딥러닝 모델을 제안한다. 제안 모델은 재난문자를 KoBERT를 통해 임베딩하고, LSTM을 통해 재난 유형별로 분류한다. [명사], [명사 + 형용사 + 동사], [모든 품사]의 3가지 품사 조합과 제안 모델, 키워드 분류, Word2Vec + 1D-CNN 및 KoBERT + FFNN의 4종류 분류 모델을 활용하여 재난문자를 분류한 결과, 제안 모델이 0.988954의 정확도로 가장 높은 성능을 달성하였다.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2007년도 The 1st International Symposium on Advanced Magnetic Materials
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• pp.287.2-287.2
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• 2007

• 한국자기학회:학술대회 개요집
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• 한국자기학회 2007년도 The 1st International Symposium on Advanced Magnetic Materials
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• pp.48.2-48.2
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• 2007