• Title/Summary/Keyword: $KI_2{\cdot}KI_{10}$

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Studies on the Production of Foods and Feeds Yeast from the Hydrolyzate of Corn Starch Cake (옥수수 전분박(澱粉粕)을 이용(利用)한 식사료(食飼料) 효모생산(酵母生産)에 관한 연구(硏究))

  • Sung, Nack-Kie;Kim, Myung-Chan;Ki, Woo-Kyung;Kim, Jong-Kyu;Yun, Han-Dae
    • Applied Biological Chemistry
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    • v.19 no.4
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    • pp.219-226
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    • 1976
  • To meet the need of protein feed and fine more efficient ways of returning waste to resources, we have carried out the study of the production of yeast for foods and feeds from the corn starch cake. The present study includes the method for acid-hydrolysis, the selection of yeast capable of utilizing hydrolyzate of the corn starch cake, and culture condition of Candida tropicalis under the liquid culture and the semisolid culture. Obtained results were as follows. 1. Hydrochloric acid was more excellent on the hydrolysis of the corn starch cake than sulfuric acid, and the yield of sugar was maximum, 57.2%, when the corn starch cake was hydrolyzed with 1.0% of hydrochloric acid at 2.0kg/cm for 30 minutes. 2. As the acid solution content was increased, more sugar was liberatedfrom the mixture, until the acid solution-substrate ratio reached 10:1. Beyond this point, no further increase was observed. To prepare the cultural medium of semisolid fermentation, a acid solution to substrate ratio of 3:1 appeared to be optimum. 3. Out of 6 yeast strains, Candida tropicalis had excellent growth on the hydrolyzate of the corn starch cake, and optimum temperature and initial pH were $30^{\circ}C$ and 6.0 respectively. 4. Optimum liquid medium of Candida tropicalis is ures 0.3%, potassium phosphate monobasic 0.15g and magnesium sulfate 0.04g in 100ml of the hydrolyzate of the corn starch cake, while optimum semisolid medium is ammonium chloride 0.4g, potassium phosphate monobasic 0.1%, magnesium sulfate 0.04%. 5. Candida tropicalis could assimilate the sugar in the hydrolyzate up to more than 88.75%, and a yield of dry yeast reached 19.13% to the corn starch cake under the liquid culture. 6. Compared to the that of the untreated corn starch cake, the cellulose content of the semisolid fermented cake decreased by 3.76% to 14.7%, whereas dry yeast contents increased by 13.89%.

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Effects of Electrolyte Concentration on Growth of Dendritic Zinc in Aqueous Solutions (수용액중 아연 덴드라이트의 성장 반응에 미치는 전해질 농도의 영향)

  • Shin, Kyung-Hee;Jung, Kyu-Nam;Yoon, Su-Keun;Yeon, Sun-Hwa;Shim, Joon-Mok;Joen, Jae-Deok;Jin, Chang-Soo;Kim, Yang-Soo;Park, Kyoung-Soo;Jeong, Soon-Ki
    • Transactions of the Korean hydrogen and new energy society
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    • v.23 no.4
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    • pp.390-396
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    • 2012
  • In order to understand the nature of dendritic zinc growth, electrochemical zinc redox reaction on nickel plate was investigated in aqueous solutions containing different concentrations, 0.2, 0.1 and 0.02 $mol{\cdot}dm^{-3}$ (M), of zinc sulfate ($ZnSO_4$) or zinc chloride ($ZnCl_2$). Zinc ion was efficiently reduced and oxidized on nickel in the high-concentration (0.2 M) solution, whereas relatively poor efficiency was obtained from the other low-concentration solutions (0,1 and 0.02 M). Cyclic voltammetry (CV) analysis revealed that the 0.2 M electrolyte solution decomposes at more positive potentials than the 0.1 and the 0.02 M solutions. These results suggested that the concentration of electrolyte solution and anion would be an important factor that suppresses the reaction of the zinc dendrite formation. Scanning Electron Microscopy (SEM) data revealed that the shape of dendritic zinc and its growing behavior were also influenced by electrolyte concentration.

Production of Poly-3-hydroxybutyrate from Xylose by Bacillus megaterium J-65 (Bacillus megaterium J-65에 의한 xylose로부터 poly-3-hydroxybutyrate 생산)

  • Jun, Hong-Ki;Jin, Young-Hi;Kim, Hae-Nam;Kim, Yun-Tae;Kim, Sam-Woong;Baik, Hyung-Suk
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1625-1630
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    • 2008
  • A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were $37^{\circ}C$ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% $(NH_4)_2SO_4$, 0.3% $Na_2HPO_4{\cdot}12H_2O$, and 0.1% $KH_2PO_4$. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of ${\beta}$-hydroxybutyric and ${\beta}$-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.

Development of a Sensor Data Service System for Telematics (텔레매틱스를 위한 센서 데이타 서비스 시스템 개발)

  • Jeon, Sang-Hun;Hong, Dong-Suk;Kim, Dong-Oh;Kim, Joung-Joon;Han, Ki-Joon
    • 한국공간정보시스템학회:학술대회논문집
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    • 2005.11a
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    • pp.141-146
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    • 2005
  • 최근 들어 텔레매틱스 분야에서 자동차의 위치 및 상태를 감시하고 관리하기 위해 무선 센서 네트워크의 활용이 크게 확장되고 있다. 기존에는 위치, 속도, 온도와 같은 이 기종 플랫폼간의 센서 데이타 수집 및 운용을 위해 독립적인 어플리케이션을 사용하고 있으며, 그러한 데이타 교환과 통합을 위하여 많은 비용이 소요되었다. OGC(OpenGIS Consortium)에서는 이러한 문제를 해결하고자 Sensor Collection Service 구현 명세를 제안하였다. Sensor Collection Service 구현 명세는 센서 데이타에 대한 질의를 처리하기 위한 표준 인터페이스를 정의하고 있으며, 질의 결과로는 센서에 대한 정보 및 센서데이타를 반환하도록 정의하고 있다. 본 논문에서는 Sensor Collection Service 표준 인터페이스를 이용하여 센서로부터 획득된 위치 및 속도, 온도 등의 다양한 데이타에 웹을 통하여 접근할 수 있도록 센서 데이타 서비스 시스템을 설계 잊 구현하였다. 따라서 사용자들은 센서 데이타 서비스 시스템을 통하여 센서의 종류와 위치에 관계없이 웹 상에서 위치 및 속도, 온도 등과 같은 다양한 센서 데이타를 획득할 수 있으며 시스템간 데이타의 교환 및 통합이 가능하다. 또한, 사용자가 쉴게 검색 및 분석할 수 있도록 Sensor Collection Service 표준 인터페이스 질의를 통하여 획득된 데이타를 센서의 위치와 측정 시간에 기반하여 보여주고 있다. 본 시스템은 다양한 센서 데이타의 획득과 타 시스템과의 데이타 교환이 용이하므로 텔레매틱스, 위치 추적 시스템, 상태 감시 시스템 등 다양한 분야에 효과적인 도구로 활용될 수 있다.이루어졌다 가능한 창조 모델로 이용될 수 있을 것으로 생각된다.}94,\;29.4{\pm}30.3,\;45.1{\pm}44$로 Mel 10군과 Mel 30군이 유의적인 감소를 보였으나(p<0.05) 이들 두 군 간의 차이는 나타나지 않았다. 이상의 결과로, 랫트에서 복강수술 후 melatonin 10mg/kg투여가 복강 내 유착 방지에 효과적이라고 생각된다.-1}{\cdot}yr^{-1}$로서 두 생태계에 축적되었다.여한 3,5,7군에서 PUFA 함량이 증가한 반면, SFA 함량은 감소하여 P/S 비율, n-3P/n-6P 비율은 증가하는 경향이었으며 이는 간장의 인지질, 콜레스테롤 에스테르, 총 지질의 지방산조성에서도 같은 경향을 볼 수 있었다.X>$(C_{18:2})$와 n-3계 linolenic acid$(C_{18:3})$가 대부분을 차지하였다. 야생 돌복숭아 과육 중의 지방산 조성은 포화지방산이 16.74%, 단불포화지방산 17.51% 및 다불포화지방산이 65.73%의 함유 비율을 보였는데, 이 중 다불포화지방산인 n-6계 linoleic acid$(C_{18:2})$와 n-3계 linolenic acid$(C_{18:3})$가 지질 구성 총 지방산의 대부분을 차지하는 함유 비율을 나타내었다.했다. 하강하는 약 4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로,

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GML Application: Developing of Data Model for Geological Information and Its Implementation (GML(Geography Markup Language) 응용: 지질주제정보 데이터 모델개발과 시험구현)

  • Lee, Ki-Won;Moon, Sun-Hee;Chi, Kwang-Hoon
    • 한국공간정보시스템학회:학술대회논문집
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    • 2003.11a
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    • pp.78-83
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    • 2003
  • OGC(Open GIS Consortium, Inc.)에서 2000년에 XML의 지리정보 처리를 위한 GML 1.0이 제안된 이후 최근 GML 3.0이 발표되기까지 국제적으로는 다양한 GIS 응용분야에서 GML을 현실 문제에 이용하고자 하는 시도가 이루어지고 있다. 현재 우리나라에서도 국가 GIS(NGIS)에서 GML을 도입하여 국내 표준안으로 추진하고자 다양한 기반연구가 진행되고 있다. 본 연구는 중요한 GIS 응용중의 하나인 지질자원 정보 분야에서 GML을 활용하기 위한 기반연구로서 우리나라의 표준 지질도(또는 수치지질정보)와 기타 지질자원 정보 인프라 구축에 적용될 수 있는 다양한 지구과학자료를 대상으로 하였다. 따라서 본 연구에서는 지질자원 분야에서의 GML 응용을 위한 기반 데이터 모델과 이를 처리하기 위한 아키텍처를 UML 기법으로 설명하고, 이를 바탕으로 시험 구현된 운용환경에서 실제 대상 지역을 선정하여, 연구지역에 대한 지질자원 정보의 GML 적용 사례를 제시하고자 한다. 이를 위한 연구 내용으로는 수치 지질도를 XML-GML의 기본 스키마구조에 따라 엔코딩한 뒤, 주제정보의 표현을 위한 XSLT, SVG 파일 변환 처리 등을 수행하였다. 결론적으로 본 연구는 지질자원 분야의 정보화 인프라 구축을 목적으로 하는 기초 시험연구로 수행되었는 바, 우리나라에서는 2001년 이후 GIS 수치 주제도로서 수치 지질도의 보급이 활성화되면서, 이 정보를 제작/공급하는 기관이나 업무에 사용하는 기관 등에서 GML 기반으로 데이터를 제작하거나 운용이 가능할 것으로 생각된다. 또한, 다른 분야에서 GIS 주제정보를 다루는 경우에도 GML을 주제정보와 연계하여 현실 문제에 적용이 가능한 창조 모델로 이용될 수 있을 것으로 생각된다.}94,\;29.4{\pm}30.3,\;45.1{\pm}44$로 Mel 10군과 Mel 30군이 유의적인 감소를 보였으나(p<0.05) 이들 두 군 간의 차이는 나타나지 않았다. 이상의 결과로, 랫트에서 복강수술 후 melatonin 10mg/kg투여가 복강 내 유착 방지에 효과적이라고 생각된다.-1}{\cdot}yr^{-1}$로서 두 생태계에 축적되었다.여한 3,5,7군에서 PUFA 함량이 증가한 반면, SFA 함량은 감소하여 P/S 비율, n-3P/n-6P 비율은 증가하는 경향이었으며 이는 간장의 인지질, 콜레스테롤 에스테르, 총 지질의 지방산조성에서도 같은 경향을 볼 수 있었다.X>$(C_{18:2})$와 n-3계 linolenic acid$(C_{18:3})$가 대부분을 차지하였다. 야생 돌복숭아 과육 중의 지방산 조성은 포화지방산이 16.74%, 단불포화지방산 17.51% 및 다불포화지방산이 65.73%의 함유 비율을 보였는데, 이 중 다불포화지방산인 n-6계 linoleic acid$(C_{18:2})$와 n-3계 linolenic acid$(C_{18:3})$가 지질 구성 총 지방산의 대부분을 차지하는 함유 비율을 나타내었다.했다. 하강하는 약 4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로, 반전거래전략을 활용하는 경우 주식투자기간은 24개월이하의 중단기가 적합함을 발견하였다. 이상의 행태적 측면과 투자성과측면의 실

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Toxicokinetics of paraquat in Korean patients with acute poisoning

  • Kim, Hak-Jae;Kim, Hyung-Ki;Lee, Hwayoung;Bae, Jun-Seok;Kown, Jun-Tack;Gil, Hyo-Wook;Hong, Sae-Yong
    • The Korean Journal of Physiology and Pharmacology
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    • v.20 no.1
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    • pp.35-39
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    • 2016
  • To conduct a kinetic study of paraquat (PQ), we investigated 9 patients with acute PQ intoxication. All of them ingested more than 20 ml of undiluted PQ herbicide to commit suicide and arrived at our hospital early, not later than 7 h after PQ ingestion. The urine dithionite test for PQ in all of the nine patients was strongly positive at emergency room. Blood samples were obtained every 30 min for the first 2~3 h and then every 1 or 2 h, as long as the clinical progression was stable among the patients for 30 h after PQ ingestion. The area under the plasma concentration-time curve ($AUC_{inf}$), which was extrapolated to infinity, was calculated using the trapezoidal rule. Toxicokinetic parameters, such as the terminal elimination half-life, apparent oral clearance, and apparent volume of distribution ($V_d/F$) were calculated. The maximum PQ concentration ($C_{max}$) and the time to reach maximum PQ concentration ($T_{max}$) were also obtained. Plasma PQ concentrations in nine patients were well described by a bi-exponential curve with a mean terminal elimination half-life of $13.1{\pm}6.8h$. $C_{max}$ and $AUC_{inf}$ were $20.8{\pm}25.7mg/l$ and $172.5{\pm}160.3h{\cdot}mg/l$, respectively. Apparent volume of distribution and apparent oral clearance were $50.9{\pm}61.3l/kg$ and $173.4{\pm}111.2l/h$, respectively. There were a significant correlation (r=0.84; p<0.05) between the PQ amount ingested and $C_{max}$. $AUC_{inf}$ also showed a significant correlation (r=0.83; p<0.05) with the PQ amount ingested. These correlations provide evidence that PQ has dose-linear toxicokinetic characteristics.

Developments of Water Treatment System by Biological Fluidized Bed for Water Reuse Aquaculture (생물학적 유동층을 이용한 어류양식 순환수의 처리씨스템 개발)

  • LEE Ki-Wan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.4
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    • pp.380-391
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    • 1993
  • The experimental study was made to propose the treatment method of wastewater in the high-density fish culture system. The BOD to COD ratios of effluents were almost same to 0.65 in the eel-farm, but were various in the farm rearing together with tilapia etc. A BOD rate curve of the eel-farm effluent could be described mathematically by the equation, $BODu=14.1(1-10^{-0.222t})+30.9(1-10^{-0.035(t-8)})$. Nitrification in Biological Fluidized Bed(BFB) system to treat the fish-farm wastewater could be reduce ammonium level up to $65{\sim}79\%$ when ammonium loading rates were between 0.014 and 0.075g $NH_4/g$ BVS-day. Nitrification efficiency was decreased by organic matters in the wastewater when ammonium loading was low(0.014 g $NH_4/g$ BVS-day). T-N removal ratios were decreased to increase loading in denitrification process, because of low C/N ratio. Based on much higher biological mass concentrations, BFB system takes many advantages of a practical viewpoint, such as stability of treatment efficiency and reduction of necessary site area for the facility, as compared with conventional treatment systems.

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Environmental Characteristics and Vegetation of Hanabusaya asiatica Habitats (금강초롱꽃 자생지의 환경특성과 식생)

  • Jang, Su-Kil;Cheon, Kyeong-Sik;Jeong, Ji-Hee;Kim, Zin-Suh;Yoo, Ki-Oug
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.497-506
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    • 2010
  • This study intended to investigate the environmental factors including soil and vegetation in order to better understand the environmental and ecological characteristics of ten different habitats of $Hanabusaya$ $asiatica$. These habitats, according to investigations, are mostly located on the slopes of mountains facing north at an altitude of 580 m to 1,396 m above sea level with angles of inclination ranging from 5 degrees to 80 degrees. A total of 146 vascular plant taxa are identified in 32 quadrates of the ten habitats. The importance value of $H.$ $asiatica$ is 8.87%, and 5 highly ranked species such as $Carex$ $siderosticta$ (8.67%), $Ainsliaea$ $acerifolia$ var. $subapoda$ (7.10%), $Calamagrostis$ $arundinacea$ (6.79%), $Athyrium$ $yokoscense$ (5.33%), $Astilbe$ $rubra$ (3.11%) are considered to be an affinity with $H.$ $asiatica$ in their habitats. Dominant species of woody plants in ten habitats are represented as $Quercus$ $mongolica$ in tree layer (T1), $Acer$ $pseudo$-$sieboldianum$ in subtree layer (T2), $Rhododendron$ $schlippenbachii$ and $Tripterygium$ $regelii$ in shrub layer (S). The degree of their average species diversity is 1.30, and that of dominance and evenness are 0.08 and 0.88, respectively. The type of soil is sandy loam, loam and loamy sand, and the average field capacity of soil is 23.95%. Their average organic matter is 12.28%, soil pH 5.79, and available phosphorus is 25.48%. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of tree layers is correlated with richness, diversity, dominance, evenness and coverage of $H.$ $asiatica$.

The Effect of Root Zone Cooling at Night on Substrate Temperature and Physiological Response of Paprika in Hot Climate (고온기 야간시간 근권냉방이 파프리카 배지온도와 생리적 반응에 미치는 영향)

  • Choi, Ki Young;Ko, Ji Yeon;Choi, Eun Young;Rhee, Han Cheol;Lee, Sung Eun;Lee, Yong-Beom
    • Journal of Bio-Environment Control
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    • v.22 no.4
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    • pp.349-354
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    • 2013
  • This study examined a technique for cooling root zone aimed at lowering substrate temperature for sweet pepper (Capsicum annum L. 'Orange glory') cultivation in coir substrate hydroponics during hot season, from the $16^{th}$ of July to $15^{th}$ of October in 2012. The root zone cooling technique was applied by using an air duct (${\varnothing}12$ cm, hole size 0.1 mm) to blow cool air between two slabs during night (5p.m. to 3a.m.). Between the $23^{rd}$ of July and $31^{st}$ of August (hot temperature period), average daily substrate temperature was $24.7^{\circ}C$ under the root zone cooling, whereas it was $28.2^{\circ}C$ under condition of no cooling (control). In sunny day (600~700 W $m^{-2}{\cdot}s^{-1}$), average substrate temperatures during the day (6a.m. to 8p.m.) and night (8p.m. to 6a.m.) were lower about $1.7^{\circ}C$ and $3.3^{\circ}C$, respectively, under the cooling treatment, compared to that of control. The degree of temperature reduction in the substrate was averagely $0.5^{\circ}C$ per hour under the cooling treatment during 6p.m. to 8p.m.; however, there was no decrease in the temperature under the control. The temperature difference between the cooling and control treatments was $1.3^{\circ}C$ and $0.6^{\circ}C$ in the upper and lower part of the slab, respectively. During the hot temperature period, about 32.5% reduction in the substrate temperature was observed under the cooling treatment, compared to the control. Photosynthesis, transpiration rate, and leaf water potential of plants grown under the cooling treatment were significantly higher than those under the control. The first flowering date in the cooling was faster about 4 days than in the control. Also, the number of fruits was significantly higher than that in the control. No differences in plant height, stem thickness, number of internode, and leaf width were found between the plants grown under the cooling and control, except for the leaf length with a shorter length under the cooling treatment. However, root zone cooling influenced negligibly on eliminating delay in fruiting caused by excessively higher air temperature (> $28^{\circ}C$), although the substrate temperature was reduced by $3^{\circ}C$ to $5.6^{\circ}C$. These results suggest that the technique of lowering substrate temperature by using air-duct blow needs to be incorporated into the lowering growing temperature system for growth and fruit set of health paprika.

Environmental Geochemical Characteristics of the Acid Mine Drainage and Stream Sediments in the Abandoned Honam Coal Mine Area, Korea (호남탄전지역 폐탄광의 산성광산배수와 하상퇴적물의 환경지구화학적 특성)

  • Park, Young-Seog;Kim, Jong-Kyun;Kim, Jin;Jang, Woo-Seog;Lee, Ki-Hyung;Han, Min-Su
    • Economic and Environmental Geology
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    • v.35 no.3
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    • pp.241-255
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    • 2002
  • The purpose of this study is to investigate environmental geochemical characteristics for the acid mine drainage and stream sediments in the abandoned Honam coal mine area. For the chemical analysis, stream winter samples were collected at 54 sites and for the mineralogical and chemical analysis, stream sediments samples were collected at 34 sites. Physical and chemical characteristics of stream water were measured at the site and chemical compositions were analyzed by ICP-AES, ICP-MS and IC. Mineralogical characteristics of stream sediments samples were determined using XRD, SEM and EDS. In physical and chemical properties, stream water shows thats pH 2.85~8.12, Eh -62~215 mV, EC 0.205~146 ms/m, ER 0.234~255 {$\Omega}{\cdot}$m, DO 0.03~1068 mg/L and TDS 10.96~1420mg/L. In chemical compositions, we obtain that K 0.118~3.184 mg/L, Mg 2.1~114.48 mg/L, Ca 2.59~l25.02 mg/L, Al 0.01~44.72 mg/L, Fe 0.108~89.49 mg/L and Na 5.45~125.41 mg/L. Stream sediments are commonly composed of quartz, illite and goethite in the result of x-ray diffraction. After heat treatment at 98$0^{\circ}C$, the hematite peaks are distinctly shown. In consideration of heavy metals (include Fe) for steam sediments, Fe ranges 22575~34713 ppm, Zn 41.66~970.3 ppm, Cd 0.52~52.07 ppm, Cu 1.25~198.5 ppm and Pb 0.43~77.35 ppm.