• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.2
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• pp.91-101
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• 2009

in the cosmetics and medical supply field as a antioxidant material. The stable nano particle emulsion of skin toner type containing VEA was prepared. To evaluate the skin permeation, experiments on VEA permeation to the skin of the ICR outbred albino mice (12 weeks, about 50 g, female) and on differences of solubility as a function of receptor formulations was performed. The analysis of nano-emulsions containing VEA 0.07 % showed that the higher ethanol contents the larger emulsions were formed, while the higher surfactant contents the size became smaller.In this study, vitamin E acetate (VEA, tocopheryl acetate), a lipid-soluble vitamin which is widely used A certain contents of ethanol in receptor phase increased VEA solubility on the nano-emulsion. When the ethanol contents were 10.0 % and 20.0 %, the VEA solubility was higher than 5.0 % and 40.0 %, respectively. The type of surfactant in receptor solution influenced to VEA solubility. The comparison between three kind surfactants whose chemical structures and HLB values are different, showed that solubility of VEA was increased as order of sorbitan sesquioleate (Arlacel 83; HLB 3.7) > POE (10) hydrogenated castor oil (HCO-10; HLB 6.5) > sorbitan monostearate (Arlacel 60; HLB 4.7). VEA solubility was also shown to be different according to the type of antioxidant. In early time, the solubility of the sample including ascorbic acid was similar to those of other samples including other types of antioxidants. However, the solubility of the sample including ascorbic acid was 2 times higher than others after 24 h. Franz diffusion cell experiment using mouse skin was performed with four nano-emulsion samples which have different VEA contents. The emulsion of 10 wt% ethanol was shown to be the most permeable at the amount of 128.8 ${\mu}g/cm^2$. When the result of 10 % ethanol content was compared with initial input of 220.057 ${\mu}g/cm^2$, the permeated amount was 58.53 % and the permeated amount at 10 % ethanol was higher 45.0 % and 15.0 % than the other results which ethanol contents were 1.0 and 20.0 wt%, respectively. Emulsion particle size used 0.5 % surfactant (HCO-60) was 26.0 nm that is one twentieth time smaller than the size of 0.007 % surfactant (HCO-60) at the same ethanol content. Transepidermal permeation of VEA was 54.848 ${\mu}g/cm^2$ which is smaller than that of particlesize 590.7 nm. Skin permeation of nano-emulsion containing VEA and difference of VEA solubility as a function of receptor phase formulation were determined from the results. Using these results, optimal conditions of transepidermal permeation with VEA were considered to be set up.

• Journal of Oriental Neuropsychiatry
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• v.31 no.1
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• pp.1-12
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• 2020

Objectives: The purpose of this study was to evaluate the reliability and validity of the instrument on pattern identification for insomnia (PIT-Insomnia) and verify the correlation between PIT-Insomnia and psychological tests. Methods: Two evaluators examined the pattern identification of the participants who met insomnia disorder diagnostic criteria of the Diagnostic and Statistical Manual of Mental Disorder, Fifth Edition (DSM-5) and took the Insomnia Severity Index (ISI) score over 15 once manually and twice using the PIT-Insomnia to measure the inter-rater and test-retest reliability. We also conducted the following surveys: the Pittsburgh Sleep Quality Index (PSQI), the Korean version of Beck's depression inventory (K-BDI), the Korean version of the State-Trait Anxiety Inventory (STAI-K), the Korean Symptom checklist-95 (KSCL-95), and the EuroQol-5 dimension (EQ-5D), to measure concurrent validity and correlation between the PTI-Insomnia and psychological tests. Results: 1. The test-retest reliability analysis of the pattern identification results showed moderate agreement, and test-retest reliability analysis of each pattern identification score showed agreements from poor to moderate. 2. The inter-rater reliability analysis of the pattern identification results via manual showed slight agreement, when analysis was performed with calibration, the inter-rater reliability analysis of the pattern identification results via manual showed fair agreement. 3. The concordance analysis between results via manual and the PIT-Insomnia showed poor agreement, when the analysis was performed with calibration, concordance analysis showed fair agreement. 4. The concordance analysis between the PIT-Insomnia and the PSQI showed positive linear correlation. 5. The concordance analysis between the PIT-Insomnia and the PSQI, K-BDI, STAI-K, KSCL-95, and EQ-5D showed that non-interaction between the heart and kidney have positive linear correlation with the K-BDI, anxiety item of KSCL-95, dual deficiency of the heart-spleen have positive linear correlation with somatization item of KSCL-95, paranoia item of KSCL-95, heart deficiency with timidity have positive linear correlation with stress vulnerability item of KSCL-95, parania item of KSCL-95, phlegm-fire harassing the heart have positive linear correlation with K-BDI, paranoia item of KSCL-95, depressed liver qi transforming into fire have positive linear correlation with the anxiety item of KSCL-95, parania item of KSCL-95, all pattern identification have negative linear correlation with EQ-5D. Conclusions: The PIT-Insomnia has moderate agreement of reliability and reflects the severity of insomnia since it has some concurrent validity with the PSQI. There are some correlations between the PTI-Insomnia with specific psychological tests, so we could suggest it can be used appropriately in the clinical situation.

• Korean Chemical Engineering Research
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• v.54 no.4
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• pp.533-542
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• 2016

We investigate fluidization characteristics of the mixture of rice husk, silica sand and rice husk ash as a preliminary study for valuable utilization of rice husk ash obtained from gasification of rice husk in a fluidized bed reactor. As experiment valuables, the blending ratio of rice husk and sand (rice husk: sand) is selected as 5:95, 10:90, 20:80 and 30:70 on a volume base. Rice husk ash was added with 6 vol% of rice husk for each experiment and air velocity to the reactor was 0~0.63 m/s. In both rice husk/sand and rice husk/sand/ash mixture, the minimum fluidization velocity (Umf) is observed as 0.19~0.21 m/s at feeding of 0~10 vol.% of rice husk and 0.30 m/s at feeding of 20 vol.% of rice husk. With increasing the amount of rice husk up to 30 vol.%, $U_{mf}$ can not measure due to segregation behavior. The mixing index for each experiment is determined using mixing index equation proposed by Brereton and Grace. The mixing index of the mixture of rice husk/sand and rice husk/sand/ash was 0.8~1 and 0.88~1, respectively. The optimum fluidization condition was found for the good mixing and separation of rice husk ash.

• Journal of the Korean Society of International Agriculture
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• v.31 no.1
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• pp.76-81
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• 2019

'Asemi' is a rice variety derived from a cross between 'Jinmibyeo' which has translucent milled rice and medium maturity and 'Cheolwon46', an elite line with high yield and early maturity by the rice breeding team at NICS, RDA in 2013. The heading date of 'Asemi' is August 1, six days earlier than the check variety 'Hwaseong'. It has 82 cm culm length and 109 spikelets per panicle. 'Asemi' is resistant to blast disease, stripe virus and tungro virus, but susceptible to other viruses and planthoppers. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and short grain shape. It has protein content (6.7%) higher than 'Hwaseong', and amylose content (19.5%) similar to 'Hwaseong'. The milled rice recovery rate of 'Asemi' is similar to that of 'Hwaseong'. However, the head rice rate of 'Asemi' is higher than that of 'Hwaseong'. Milled rice yield of 'Asemi' is 5.23 MT/ha in ordinary cultivation. ' Asemi' could be adaptable to the wide region of tropical Asia (Registration No. 5639).

• Journal of the Korean Society of Radiology
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• v.82 no.3
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• pp.562-574
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• 2021

MRI has the advantages of having excellent soft-tissue contrast and providing functional information without any harmful ionizing radiation. Although previous technical limitations restricted the use of chest MRI, recent technological advances and expansion of insurance coverage are increasing the demand for chest MRI. Recognizing the need for guidelines on appropriate use of chest MRI in Korean clinical settings, the Korean Society of Radiology has composed a development committee, working committee, and advisory committee to develop Korean chest MRI justification guidelines. Five key questions were selected and recommendations have been made with the evidence-based clinical imaging guideline adaptation methodology. Recommendations are as follows. Chest MRI can be considered in the following circumstances: for patients with incidentally found anterior mediastinal masses to exclude non-neoplastic conditions, for pneumoconiosis patients with lung masses to differentiate progressive massive fibrosis from lung cancer, and when invasion of the chest wall, vertebrae, diaphragm, or major vessels by malignant pleural mesothelioma or non-small cell lung cancer is suspected. Chest MRI without contrast enhancement or with minimal dose low-risk contrast media can be considered for pregnant women with suspected pulmonary embolism. Lastly, chest MRI is recommended for patients with pancoast tumors planned for radical surgery.

• Tuberculosis and Respiratory Diseases
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• v.49 no.3
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• pp.332-342
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• 2000

Background : The importance of pro-inflammatory cytokines, especially tumor necrosis factor $\alpha$ (INF-$\alpha$) and interleukin-1$\beta$ (IL-1$\beta$), have been extensively documented in the generation of inflammatory lung disease. Lung epithelial cells are also actively involved in initiating and maintaining inflammation by producing pro-inflammatory mediators. Understanding the mechanism of pro-inflammatory cytokine expression in lung epithelial cells is crucial to the development of new therapeutic modalities for inflammatory lung disease. Transcription of most pro-inflammatory cytokines is dependent on the activation of NF-${\kappa}B$. However, the relationship between pro-inflammatory cytokine expression and NF-${\kappa}B/I{\kappa}B$ pathway in lung epithelial cells is not clear. Methods : BEAS-2B, A549, Na-H157, NCI-H719 cells were stimulated with IL-$1{\beta}$ or TNF-$\alpha$ at various times, and then IL-8 and TNF-$\alpha$mRNA expressions were assayed by Northern blot analysis. IL-$1{\beta}$ or TNF-$\alpha$-induced NF-${\kappa}B$ activation was assessed by the nuclear translocation of p65 NF-${\kappa}B$ subunit. The degradation of $I{\kappa}B{\alpha}$ and $I{\kappa}B{\beta}$ by IL-$1{\beta}$ or TNF-$\alpha$stimulation was assayed by Western blot analysis. The phosphorylation of $I{\kappa}B{\alpha}$ was evaluated by Western blot analysis after pre-treating cells with proteasome inhibitor followed by IL-$1{\beta}$ or TNF-$\alpha$ stimulation. The basal level of IKK $\alpha$ expression was evaluated by Western blot analysis. Results: $I{\kappa}B{\alpha}$ and $I{\kappa}B{\alpha}$ was rapidly degraded after 5 minutes of incubation with IL-$1{\beta}$ or TNF-$\alpha$ in BEAS-2B, A549, and NCI-H157 cells. The activation of NF-${\kappa}B{\alpha}$ and the induction of IL-8 and TNF-$\alpha$ mRNA expression were observed by IL-$1{\beta}$ or TNF-$\alpha$ stimulation in these cells. In contrast, neither the changes in NF-${\kappa}B/I{\kappa}B$ pathway nor IL-8 and TNF-$\alpha$mRNA expression was induced by IL-$1{\beta}$ or TNF-$\alpha$ stimulation in NCI-H719 cells. IL-$1{\beta}$ and TNF-$\alpha$-induced $I{\kappa}B$ phosphorylation was observed in BEAS-2B, A549, and NCI-H157 cells, but not in NCI-H719 cells. The basal level of IKK$\alpha$ expression was not different between cell. Conclusion : NF-${\kappa}B/I{\kappa}B$ pathway plays an important role in the expression of pro-inflammatory cytokine in most lung epithelial cells. The absence of the effect on NF-${\kappa}B/I{\kappa}B$ pathway in NCI-H719 cells sæms to be due to the defect in the intracellular signal transduction pathway upstream to IKK.

• The Korean Journal of Nuclear Medicine
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• v.39 no.1
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• pp.34-43
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• 2005

Purpose: $^{99m}Tc$-sestamibi(MIBI) and $^{99m}Tc$-tetrofosmin have been used as substrates for P-glycoprotein (Pgp) and multidrug resistance associated protein (MRP), which are closely associated with multidrug resistance of the tumors. To understand different handling of radiotracers in cancer cell lines expressing Pgp and MRP, we compared cellular uptakes of $^{99m}Tc$-MIBI and $^{99m}Tc$-tetrofosmin. The effects of cyclosporin A (CsA), well-known multidrug resistant reversing agent, on the uptake of both tracers were also compared. Materials and Methods: HCT15/CL02 human colorectal cancer cells for Pgp expressing cells, and human non-small cell lung cancer A549 cells for MRP expressing cells, were used for in vitro and in vivo studies. RT-PCR, western blot analysis and immunohistochemistry were used for detection of Pgp and MRP. MDR-reversal effect with CsA was evaluated at different drug concentrations after incubation with MIBI or tetrofosmin. Radioactivities of supernatant and pellet were measured with gamma well counter. Tumoral uptake of the tracers were measured from tumor bearing nude mice treated with or without CsA. Results: RT-PCR, western blot analysis of the cells and irnrnunochemical staining revealed selective expression of Pgp and MRP for HCY15/CL02 and A549 cells, respectively. There were no significant difference in cellular uptakes of both tracers in HCT15/CL02 cells, but MIBI uptake was slightly higher than that of tetrofosmin in A549 cells. Co-incubation with CsA resulted in a increase in cellular uptakes of MIBI and tetrofosmin. Uptake of MIBI or tetrofosmin in HCT15/CL02 cells was increased by 10- and 2.4-fold, and by 7.5 and 6.3-fold in A549 cells, respectively. Percentage increase of MIBI was higher than that of tetrofosmin with CsA for both cells (p<0.05). In vivo biodistribution study showed that MIBI (114% at 10 min, 257% at 60 min, 396% at 240 min) and tetrofosmin uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were progressively increased by the time, up to 240 min with CsA. But increases in tumoral uptake were not significantly different between MIBI and tetrofosmin for both tumors. Conclusion: MIBI seems to be a better tracer than tetrofosmin for evaluating MDR reversal effect of the modulators in vitro, but these differences were not evident in vivo tumoral uptake. Both MIBI and tetrofosmin seem to be suitable tracers for imaging Pgp- and MRP-mediated drug resistance in tumors.

• Journal of Korean Society of Forest Science
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• v.29 no.1
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• pp.54-101
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• 1976

This study was conducted on the major factors affecting soil erosion and surface run-off. In order to investigate the processes and mechanisms of soil erosion on denuded forest-land in Korea, and to systematize the magnitudes of influences and interactions between individual factors, the five major factors adopted in these experiments are soil textures (coarse sand and clay loam), slope steepness ($10^{\circ}$, $20^{\circ}$, $30^{\circ}$ and $40^{\circ}$), rainfall intensities (50, 75 and 100mm/hr), slope mulching methods (bare, coarse straw-mat mulching, grass mulching and anti-erosion liquid mulching) and vegetation densities (sparse, moderate and dense). The processes and mechanisms of soil erosion, and the effects of mulchings on soil erosion as well as surface run-off rates were studied algebraically with four parts of laboratory experiments under the simulated rainfall and another part of field experiment under the natural rainfall. The results in this study are summarized as follows: 1. Experiment factors and surface run-off rates The surface run-off rates under the natural rainfall were resulted about 24.7~28.7% from the bare slopes, about 14.0~16.4% from the straw-mat mulched slopes, about 7.9~9.1% from the liquid mulched slopes, and about 5.6~7.2% from the grass mulched slopes respectively. The surface run-off rates under the simulated rainfall differed greatly according to the rainfall intensity and the mulching method. 2. Magnitudes of influences and interactions of the individual factor on the surface run-off rates. The experimental analyses on the major factors(soils, slopes, rainfalls, mulchings and vegetations) affecting the rates of surface run-off, show that the mean differences of surface run-off rate are significant at 5% level between the soil texture factors, among the slope steepness factors, among the rainfall intensity factors, among the mulching method factors, and among the vegetation density factors respectively. The interactions among the individual factor have a great influence(significant at 1% level) upon the rate of surface run-off, except for the interactions of the factors between soils and slopes; between slopes and vegetations; among soils, slopes and rainfalls; and among soils, slopes and mulchings respectively. On the bare slopes under the simulated rainfall, the magnitude of influences of three factors(soils, slopes and rainfalls) affecting the rate of surface run-off is in the order of the factor of rainfalls, soils and slopes. The magnitude of influences of three factors (soils, rainfalls and mulchings) affecting the rate of surface run-off, on the mulched slopes under the simulated rainfall is in the order of the factor of mulchings, rainfalls and soils and that of influences of the factor of soils, slopes and mulchings is in the order of the factor of mulchings, soils and slopes. On the vegetation growing slopes under the simulated rainfall, the magnitude of influences of three factors (soils, slopes and vegetations) affecting the rate of surface run-off is in the order of the factor of vegetations, soils and slopes. In the same condition of treatments on the field experiment under the natural rainfall, the order of magnitude of influences affecting the rate of surface run-off is the factor of mulchings, soils and slopes. 3. Experiment factors and soil losses The soil losses of the experiment plots differed according to the factors of soil texture, slope steepness, rainfall intensity and mulching method. The soil losses from the coarse soil were increased about 1.1~1.3 times as compared with that of fine soil under the natural rainfall, while the soil losses from the fine soil were increased about 1.2~1.3 times compared with that of coarse soil under the simulated rainfall. The equation of $E=aS^b$ (a, b are constant) between the slope steepness (log S) and soil losses (log E) under the simulated rainfall were developed. The equation of $E=aI^b$ (a, b are constant) between the rainfall intensity (log I) and soil losses (log E) were developed, and b values have a decreasing tendency according to the increase of the slope steepness and rainfall intensity. The soil losses under the natural rainfall were appeared about 38~41% from the coarse straw-mat mulched slopes, about 20~22% from the liquid mulched slopes, about 14~15% from the grass mulched slopes as compared with that of the bare slopes respectively. The soil loss from the vegetation plots showed about 7.1~16.4 times from the sparse plot, about 10.0~17.9 times from the moderate plot and about 11.1~28.1 times from the dense plot as compared with that of the bare slopes. 4. Magnitudes of influences and interactions of the individual factor on the soil erosion. The experimental analyses on the major factors(soils, slopes, rainfalls, mulchings and vegetations) affecting the soil erosion, show that the mean differences of soil losses are highly significant between the soil texture factors, among the slope steepness factors, among the rainfall intensity factors, among the mulching method factors and among the vegetation density factors respectively. The interactions among the individual factor have mostly great influences upon the soil erosion. The magnitude of influences of three factors (soils, slopes and rainfalls) affecting the soil erosion on the bare slopes under the simulated rainfall is in order of the factor of rainfalls, soils and slopes. On the mulched slopes under the simulated rainfall, the magnitude order of influences of three factors(soils, rainfalls and mulchings) affecting the soil erosion is the factor of mulchings, rainfalls and soils, and the order of influences of factor of soils, slopes and mulchings is the factor of mulchings, soils and slopes. On the vegetation growing slopes under the simulated rainfall, the magnitude of influences of three factors (soils, slopes and vegetations) affecting the soil erosion is in the order of the factor of slopes. vegetations and soils. In the same condition of treatments on the field experiment under the natural rainfall, the order of magnitude of influences of three factors (soils, slopes and mulchings) affecting the soil erosion is the factor of mulchings, of slopes and of soils.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.387-394
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• 2017

Temperature-dependent oviposition model and life table parameters of Paromius exiguus (Distant), the causal agent of 'pecky' rice grain were examined at eight constant temperatures (17.5, 20, 22.5, 25, 27.5, 30, 32.5, and $35{\pm}^{\circ}C$) and a photoperiod of 14:10 (L:D) h. Unit functions of the oviposition model were developed and life table parameters were estimated. The longevity of P. exiguus adults decreased with increasing temperature (123.8 days at $17.5^{\circ}C$ and 23.6 days at $32.5^{\circ}C$). Total fecundity was highest at $30^{\circ}C$ (585.2 eggs/female) and lowest at $17.5^{\circ}C$ (21.5 eggs/female). In order to develop a temperature-dependent oviposition model, adult aging-rate, temperature-dependent fecundity, age-specific survival rate, and age-specific cumulative oviposition rate equations were estimated. All unit equations ($r^2=0.92{\sim}0.98$) except for the temperature-dependent fecundity equation ($r^2=0.83{\sim}0.85$), described oviposition characteristics of P. exiguus adequately. Life table parameters of P. exiguus were estimated at various constant temperatures. Net reproduction rate ($R_0$) was highest at $30^{\circ}C$ (118.21). Mean generation time (T) was shortest at $32.5^{\circ}C$ (32.99 days) and doubling time (Dt) was shortest at $30^{\circ}C$ (5.69 days). The highest values of intrinsic rate of increase ($r_m$) and finite rate of increase (${\lambda}$) were 0.122 and 1.129 at $30^{\circ}C$, respectively.

• Tuberculosis and Respiratory Diseases
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• v.48 no.2
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• pp.166-179
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• 2000

Background: The main reason for the failure of anti-cancer chemotherapy is the build up of resistance by cancer cells to apoptosis. The activation of NF-${\kappa}B$ in many cancer cell lines is reported to be underlying mechanism behind the build up of resistance of cancer cells to apoptosis. However, this relationship varied depending on the cells used in the experiments. In this study, the role of NF-${\kappa}B$ activation in the TNF-$\alpha$-induced apoptosis in lung cancer cell line was evaluated. Methods: NCI-H157 cells were used in all experiments. Cells were exposed to a high dose of TNF-$\alpha$(20 ng/ml) for 24 or 48 hours with or without blocking NF-${\kappa}B$ activation. TNF-$\alpha$-induced activation of NF-${\kappa}B$ was inhibited either by overexpression of $I{\kappa}B{\alpha}$-super repressor($I{\kappa}B{\alpha}$-SR) or by pre-treatment with proteasome inhibitor. Cell viability and apoptosis were evaluated with MTT assay and Western blot analysis for PARP fragment, respectively. Results: Cell viability of NCI-H157 cells was not affected by TNF-$\alpha$ treatment alone; however, combined treatment with TNF-$\alpha$ and cycloheximide reduced cell viability significantly, indicating that resistance to TNF-$\alpha$ is mediated by the new proteins synthesized after TNF-$\alpha$ stimulation. To evaluate the role of NF-${\kappa}B$ in the transcription of anti-apoptotic proteins. delete NF-${\kappa}B$ activation was inhibited before TNF-$\alpha$ stimulation. as described above. $AD5I{\kappa}B{\alpha}$-SR-transduction inhibited TNF-$\alpha$-induced nuclear translocation of p65. TNF-$\alpha$-induced cell death and apoptosis increased after inhibition of TNF-$\alpha$-induced activation of NF-${\kappa}$ by methods. Conclusion: These results suggest that TNF-$\alpha$-induced activation of NF-${\kappa}B$ may be closely related to the acquisition of the resistance to TNF-$\alpha$-induced apoptosis in lung cancer cells. Therefore. blocking of NF-${\kappa}B$ pathway can be a useful therapeutic modality in the treatment of lung cancer.