• 동국한의학연구소논문집
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• 제1권
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• pp.237-249
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• 1992

In order to study tile effects of moxibustion on renal failure induced by $HgCl_2$ in rats aminal experiments were carry out. The moxibustion was treat into Sin-soo locus(VII23), We-soo locus(VII21) and Sin-soo + We-soo loci these loci were correspond to human body. Experimental group were divided into 4 groups : lontrol group, moxibustion groups treat on Sin-Soo and We-Soo, Sin-Soo + We-Soo group. The obtained results were as follows : 1. The concentration of serum sodium were inreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo loci showed increased on the 1st and 3rd days. All three experimental groups showed a significant increased on 3rd day. 2. The concentration of serum potassium were decreased in all the group, compared with those of the control group. Sin-Soo, We-Soo showed a significant decrease on 3rd day. Sin-Soo + We-Soo showed a little decrease but do not significant decrease. 3. The levels of serum total protein were increased in all the group, compare with those of the control group. Sin-Soo, We-Soo show a significant increased or 3rd day. Sin-Soo + We-Soo do not showed a significant increased. 4. The levels of serum albumin were decreased in all the groups, compared with those of the control group. Sin-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. We-Soo does not showed a significant decrease. 5. The concentration of serum blood urea nitrogen were decreased in all the groups, compared with those of the control group. Sin-Soo, We-Soo, Sin-Soo + We-Soo showed a significant decrease on 3rd day. 6. The levels of serum alkaline phosphatase were decreased in all the group, compared with those of the control groups. Sin-Soo, We-Soo showed a significant decrease on 3rd day Sin-Soo + We-Soo not a significant decrease. In conclusion, the result of this study seemed to effect on the treatment for renal failure induced by $HgCl_2$.

• 대한화학회지
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• 제32권5호
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• pp.476-482
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• 1988

$Hg^{2+}$ 수용액내에서 cis-[$Co(en)_2YCl$]$^{r+}$ (Y = $NH_3$, $NO_2^-$, NCS$^-$, $H_2O$)의 아쿠아 반응속도를 UV/vis 분광광도계로 측정하여 반응속도식을 결정하였으며, 확장분자궤도법(Extended Huckel Molecular Orbital Method)에 의해 Co(Ⅲ) 전이금속착물의 양자화학적값을 계산하고 실험에서 구한 반응속도와의 상관성을 조사하여 촉매가 관련한 타당한 반응메카니즘을 제안하였다. 본 연구에서 Co(Ⅲ) 전이금속착물과 $Hg^{2+}$에 대하여 각각 1차반응이고, 반응속도는 Co(Ⅲ) 중심금속의 알짜전하 크기와 병행성이 있으며, 이들의 반응속도는 Y의 종류에 따라 $NH_3$ < NCS$^-$ < H$_2$O < NO$_2^-$의 순으로 증가되었다. 그리고 이들 생성물의 cis-이성질체가 약 95${\%}$, trans-이성질체가 약 5${\%}$정도 생성되었다. 또한 EHMO법에 의해 계산된 알짜전하와 결합차수로부터 본 반응계는 결합깨어짐보다 결합형성이 우선되어 H$_2$0가 Co(Ⅲ) 중심금속에 공격하는 단계가 속도결정 단계가 됨을 알았다. 이러한 사실과 실험에서 구한 속도자료 그리고 활성화파라메타값으로부터 Id 메카니즘으로 진행되는 타당한 반응메카니즘을 제안하였다.

• 미생물학회지
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• 제38권2호
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• pp.74-80
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• 2002

단일 탄소원 및 에너지원으로 benzoate를 이용하는 Acinetobacter sp. KS-1에서 분리 정제한 catechol 1,2-dioxygenase (Cl,2O)의 특성과 아미노산 서 열을 분석하였다. Cl,2O는 catechol과 4-methylcatechol에 대해서 효소활성을 나타내었으며, 활성 최적온도는 $35^{\circ}C$이고, 활성 최적 pH는 7.5-9.0의 범위 내에 있었다. 효소활성 저해제로서 은, 수은, 그리고 구리는 Acinetobacter sp. KS-1의 Cl,2O 활성을 억제하였다. SDS-PAGE에 의해 측정된 Cl,2O의 분자량은 약 36 kDa 였으며, N-말단 아미노산 서 열을 분석한 결과, $^{1}MNYQQIDALVKQMNVDTAKG^{20}$로 Acinetobacter radioresistens의 Cl,2O와 95%의 유사성을 보여주었다. In-gel 아미노산 서열 분석을 위하여 trypsin 처리와 peptide mapping을 실시하였다. MALDI-TOF를 이용하여 trypsin으로 처리된 세 개의 peptide flagmen써 분자량을 분석한 결과 966.3 Da, 2081.7 Da, 그리고 1933.8 Da으로 각각 나타났는데, 이는 A. radioresistens의 Cl,2O와 내부 서 열$^{1}SQSDFNLRR^{9}\, ^{1}HGNRPSHVHYFNSAPGYR^{18}\, ^{1}TIEGPLYVAGAPESVGFAR^{19}$ 이 일치하는 것으로 분석되었다. N-말단 서열과 내부 서열을 바탕으로 primer를 제작하여 polymerase chain reaction을 실시하였다.

• 대한자원환경지질학회:학술대회논문집
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• 대한자원환경지질학회 2003년도 춘계 학술발표회 논문집
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• pp.20-23
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• 2003

Low-sulfidation style ore deposits, the major source of Au, Ag, and Hg, are formed from neutral-pH, reduced hydrothermal solutions close to equilibrium with their host rocks. The waters are low-salinity (〈1 wt % NaCl equiv.) but relatively gas rich (1-2 wt % $CO_2$), and are largely meteoric water. However, the contribution of magmatic components to the epithermal system, its temporal importance, and its relation to the source of ore metals are largely controversial. (omitted)

• 공업화학
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• 제27권3호
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• pp.335-341
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• 2016

본 연구에서는 지상 및 미소중력환경하에서 물리적 승화법 공정에서의 확산-대류유동에 미치는 불순물의 영향을 이론적으로 $Hg_2Cl_2-I_2$ 시스템에 적용하여 규명하는 것이다. 이론적 해석은 증기상에서 확산-대류 흐름, 열 및 물질전달을 속도 벡터 흐름, 유선, 온도, 농도 분포를 통하여 제시된다. 결정 영역에서의 전체 몰플럭스는 중력가속도와 성분 $I_2$, 불순물에 상당히 민감하게 반응한다. 성분 $I_2$을 증가시켰을 때, 농도 대류효과는 확산-대류 유동흐름을 안정화시키는 경향이 있다. 지상중력가속도의 0.001환경에서는 유동흐름은 1차원포물선의 흐름 구조를 나타내며, 확산지배형태를 보여주고 있다. $10^{-3}$지상중력가속도 이하에서는 대류 영향은 무시할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제7권4호
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• pp.250-257
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• 1997

A new sensing system based on the immobilization of luminescent bacteria, photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate were very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb$(NO_3)_2$), $NiCl_2$, $CdCl_2$, 50 ppm for $NaASO_2$, 0.1 ppm for $HgCl_2$, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of $-80^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) was optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol. A rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

• 대한화학회지
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• 제21권5호
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• pp.372-378
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• 1977

물 중에 ppb 程度로 존재하는 구리, 납, 카드뮴 및 아연을 定量하기 위하여 다음과 같은 두가지 分析法을 開發하여 검토하였다. (A) 試料水 500ml에 pH 10인 시트로산암모늄 10ml 및 1${\%}$ DDTCml를 加하여 10ml의 클로로포름으로 세번 抽出하여, 분리된 有機層에 10ml의 0.05N $HCl-4{\times}10^{-4}mol/l\;HgCl_2$로서 逆抽出을 한 후, 水層에 2N KCl 2ml 를 加하고 10ml의 클로로포름으로 두번 씻은 다음 矩形波폴라로그램을 기록한다. (B) 試料水 500ml에 1${\%}$ oxine 2ml를 加하여 pH 10에서 10ml의 클로로포름으로 3回 抽出한다. 분리한 有機層을 0.20 N HCl 10ml로서 逆抽出하여 바로 폴라로그램을 기록한다. 이 分析法으로 ppb程度의 구리, 납, 카드뮴 및 아연이온을 相對誤差 10${\%}$ 이내에서 定量이 可能하였으며 (B) 方法은 아연의 定量이 不可能하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제28권6호
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• pp.413-420
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• 2002

Mercury is one of the most frequently used heavy metal in dental clinic. Mercury poisoning rises up when someone is exposed to mercury chronically. In 1818, Amalgam was used for dental restorative procedure, and after then study about mercury toxicity has begun. Clinical signs of mercury toxicity in oral & maxillofacial area were increases of salivation, metallic taste, swelling and pain of tongue, redness and ulceration of oral mucosa, and increased mobility and loss of teeth. After we injected mercury($HgCl_{2}$) into intraperitoneum of rat, studied about histopathological changes of submandibular gland cell. Experimental group was divided into two groups by amount of mercury. (Group 1 was 0.5mg/Kg of mercury injection, group 2 was 1.0mg/Kg of mercury injection.) 1. After 3days of intraperitoneal injection, black granules were observed at macrophage cell in both group. In group 2, author found hyperchromatism of nucleus, and vacuolization of cellular matrix and nucleus of acinar cell. 2. After 1week of intraperitoneal injection, author found severe vacuolization of nucleus and cellular matrix, and irregular granules around nuclear membrane at mucous cell and serous cell in both group. Vacuolization of nucleus and cellular matrix was seen at duct cell in group 2. 3. After 2weeks of intraperitoneal injection, author could found severe vacuolization of cellular matrix, and sometimes nucleus was positioned in central area of cellular matrix at mucous and serous cell in both group. Vacuolization of nucleus and cellular matrix was found at vascular endothelial cell in group 2. 4. After 4weeks of intraperitoneal injection, destruction and distortion of gland cells were distinct. Vacuolization and destruction of nucleus and cellular matrix was found at duct cell in group 2. After intraperitoneal injection of mercury, we found equanimity of mercury and destruction of cellular matrix at serous cell, mucous cell, and duct cell of submandibular gland. So, we thought that metallic taste of mercury poisoning patient would be due to excretion of saliva containing mercury.

• 한국식품영양과학회지
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• 제23권6호
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• pp.908-915
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• 1994

수은($HgCl_2$) 중독 흰쥐에서 마늘즙의 antidotic effect를 검토하기 위한 4주간 실험에서 흰쥐 체중 kg당 주 1회 수은을 2.5mg 투여하고 아울러 마늘즙을 매일 식이의 2%를 투여한 흰주의 성장율이 수은만을 투여한 흰주 보다 약 30% 증가(개선)되었다. 마늘즙 단독 투여군은 오히려 대조군 보다 상장율이 15% 증가되어 마늘이 일반 tonic 효과를 나타냈다. 혈액검사에서 수은 투여군(M)은 GPT, GOT 상승과 cholesterol, triglyceride, Alk.p의 병적 감소를 보이고, 무기수은 중독 표기 기관인 신장에 대한 그의 기능검사 지표항목인 BUN과 uric산 creatinine의 측정값들이 수은 투여로 상승했으나 마늘즙을 투여(MG군)하여 이들 측정값이 유의적으로 감소하였다. 혈액과 신장의 수은 함량 조사에서 대조군(0.015, 0.02ppm)과 비교한 수은만 투여군이 각각 0.46, 0.51ppm로 높으나 수은과 함께 마늘즙 투여군은 각각 0.3, 0.33ppm으로 유의적인 감소를 보였다. 따라서 마늘즙의 non protein sulfur amino acid가 수은에 대하여 antidotic effect을 갖는 것으로 관찰되었다.

• 환경위생공학
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• 제6권2호
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• pp.109-121
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• 1991

For the analysis of mercury in blood and urine, many literatures have been reffered and many kinds of reducing agents for mercury reduction and many acids for pretreatmr are known to be varied. $So HNO_{3}$ and $H_{2}SO_{4}$ among acids and $SnCl_{2}$ and $NaBH_{4}$ as a reducing agent being chosen for the establishment of more efficient and less erroneous analysis, and comparing the absorbance by using vapor generator, the results are followings. 1. The difference of absorbance from concentration of $HNO_{3}$ and $H_{2} SO_{4}$ was not nearly found when mercury reduced by $NaBH_{4}$ after pretreatment. But for more precise analysis, conc acid treatment need to be used. 2. Higher absorbance was shown by using conto acid treatment (P<0.005) when mercury reduced by $NaBH_{4}$ after treating acid primer. And sample which has 99.5% reliability in T-test, treated by conc $H_{2}SO_{4}$(P<0.005) was shown higher absorbance than treat by CORC $HNO_{3}$. 3. The difference of absorbance was not in the slightest in higher 0.1 w/v% $NaBH_{4}$ proved by uruskal-wallis H-Test 4. Some difference of absorbance in $SnCl_{2}$(P<0.005) having 99.5% reliability was found but there was no difference in these 20 w/v% , 25 w/v% and 30 w/v% SnCl$_{2}$ by the experiment of T-test. 5. According to these test results, organic materials were much affect the absorballce when reducing mercury by using $SnCl_{2}$ rather than by $NaBH_{4}$. For bio sample which is contained various organic substances, reduclng agent $NaBH_{4}$ is a lot more efficient to reduce the error then $SnCl_{2}$. 6. analytic method for this study is as following. 7. As the recovery test was done by this, the rate of recovery was shown form 94% to 100.7% .