• 한국양식학회지
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• 제11권4호
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• pp.557-564
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• 1998

무지개송어의 초대배양 간세포에 있어서 Vitellogenin (VTG)의 합성 유도는 전기영동적 수법에 의해 행하였다. 간세포는 7일 동안 phositively charged dish를 이용한 배양으로 단층 확산되었다. 배양 7일의 간세포 생존율은 $E_2$의 유무에 따라 각각 20.7% 및 23.6%가 감소하였으며, DNA함유량도 각각 13.7% 및 14.0%가 감소하였다. $E_2$ 첨가군과 대조군간에는 생존율과 DNA 함유량에 있어서는 유의한 차이가 나타나지 않았다. 그리고, 총 단백질에 대한 VTG의 비율은 $E_2$$10^{-6}$ M의 첨가시에 최대치를 나타내었다. 그러나, 고농도($10^{-5}$M)에서는 오히려 감소하는 경향을 나타내었다.

• 한국초전도ㆍ저온공학회논문지
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• 제24권3호
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• pp.30-34
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• 2022

Effects of electron beam (EB) irradiation on the mechanical strength of Cu (conducting sheath) and Nb (diffusion barrier) of Cu/Nb/MgB₂ superconducting was investigated. Wire- and tape-type Cu/Nb/MgB₂ samples were irradiated at E-beam energy of 2.5 MeV and 5 mA and a maximum E-beam dose was 5×10¹⁷ e/m². The hardness value of Cu and Nb region was measured by the Vickers micro-hardness method. In the case of the wire sample, the hardness of Cu and Nb increased proportionally as the dose was increased up to 5×10¹⁷ e/m², whereas in the case of the tape sample, the hardness increased up to a dose of 0.5×10¹⁷ e/m², and decreased slightly 5×10¹⁷ e/m². The hardness increase of Cu and Nb is believed to be due to the decrease of the deformability of Cu and Nb due to the defects formed inside the materials by E-beam irradiation.

• Archives of Pharmacal Research
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• 제23권4호
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• pp.267-282
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• 2000

Cytochrome P45O (CYP) 2E1 catalyzes the metabolism of a wide variety of therapeutic agents, procarcinogens, and low molecular weight solvents. CYP2E1-catalyzed metabolism may cause toxicity or DNA damage through the production of toxic metabolites, oxygen radicals, and lipid peroxidation. CYP2E1 also plays a role in the metabolism of endogenous compounds including fatty acids and ketone bodies. The regulation of CYP2E1 expression is complex, and involves transcriptional, post-transcriptional, translational, and post-translational mechanisms. CYP2E1 is transcriptionally activated in the first few hours after birth. Xenobiotic inducers elevate CYP2E1 protein levels through both increased translational efficiency and stabilization of the protein from degradation, which appears to occur primarily through ubiquitination and proteasomal degradation. CYP2E1 mRNA and protein levels are altered in response to pathophysiologic conditions by hormones including insulin, glucagon, growth hormone, and leptin, and growth factors including epidermal growth factor and hepatocyte growth factor, providing evidence that CYP2E1 expression is under tight homeostatic control.

• Asia pacific journal of information systems
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• 제33권3호
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• pp.792-811
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• 2023

Recently, Web 3.0 services such as virtual currency, block chain, and NFT are attracting public attention. Users who depended on platforms are moving away from being dependent on service providers and moving to Web 3.0 services. Representative services of Web 3.0 include P2E (Play to Earn) and M2E (Move to Earn). In the case of P2E, various studies have been conducted as it is widely covered in the press and media, but research on M2E is relatively lacking. This study attempts to identify the intention to use M2E by using the expanded technology acceptance model. External factors were selected based on M2E's own characteristics and personal characteristics, a research model was designed, and the proposed hypotheses were verified through factor analysis and goodness of model fit. As a result of the study, it was confirmed that profitability, innovativeness, and self-expression had a positive effect on perceived characteristics, and that perceived usefulness, perceived enjoyment, and social influence had a positive effect on intention to use. Through the research results, practical implications for efficient service operation that meets the needs of users are presented to M2E platform providers.

• Molecules and Cells
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• 제21권2호
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• pp.294-301
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• 2006

We have previously reported that phosphorylation of eukaryotic elongation factor 2 (eEF2) is related to the differentiation of chick embryonic muscle cells in culture. In the present study, we found that eEF2 phosphorylation declined shortly after induction of differentiation of L6 myoblasts, when the cells prepare for terminal differentiation by withdrawing from the cell cycle. This decrease in phosphorylation was prevented by inhibitors of phosphoinositide 3-kinase (PI3-kinase) that strongly inhibit myoblast differentiation. We hypothesized that PI3-kinase plays an important role in myoblast differentiation by regulating eEF2 phosphorylation in the early stages of differentiation. To test this hypothesis, myoblasts were synchronized at in $G_2/M$ and cultured in fresh differentiation medium (DM) or growth medium (GM). In DM the released cells accumulated in $G_0$/$G_1$ while in GM they progressed to S phase. In addition, cyclin D1 was more rapidly degraded in DM than in GM, and eEF2 phosphorylation decreased more. Inhibitors of PI3-kinase increased eEF2 phosphorylation, but PI3-kinase became more activated when eEF2 phosphorylation declined. These results suggest that the regulation of L6 myoblast differentiation by PI3-kinase is related to eEF2 phosphorylation.

• 한국어류학회지
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• 제8권1호
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• pp.49-55
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• 1996

무지개송어, Oncorhynchus mykiss 난황자어의 부상기 이전까지의 부화일수에 따른 전장(TL)성장식은 $TLt=2.7e^{-1.24{\cdot}e^{-0.11t}}(r^{2}=0.66)$, 전중(TW)성장식은 $TWt=1.8e^{-2.03{\cdot}e^{-0.11t}}(r^{2}=0.66)$, 체세포중량(SW)성장식은 $SWt=1.8e^{-5.41{\cdot}e^{-0.13t}}(r^{2}=0.83)$로 나타났다. 부화일수 경과에 따른 난황장, 난황고, 난황부피는 감소를 나타내는 직선식이였다. 전중-전장, 체세포중량-전장, 난황장-전장, 난황고-전장, 난황부피-전장, 난황중-전장, 난황중-전중, 난황중-난황고 및 난황중-난황장의 성장형질간의 상대성장식은 부화일 경과에 따라 난황홉수 경향을 반영하였다.

• 융합보안논문지
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• 제18권1호
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• pp.13-18
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• 2018

암호시스템에서 효율성은 매우 중요한 요소 중의 하나이다. 천정희 외 3인은 이산대수 문제에 기반하는 암호 시스템에서 지수승 연산 속도를 높이기 위해 새로운 지수 형태를 제안하였다. 제안된 변형은 고정된 원소 ${\alpha}$와 작은 해밍 웨이트를 가지는 두 원소 $e_1$, $e_2$에 대해 $e_1+{\alpha}e_2$로 표현되며 스플릿 지수라 불린다. 그들은 $e_1$, $e_2$를 각각 $Z_p$의 부분집합이면서 언밸런스드 부분집합인 $S_1$, $S_2$에서 선택하였다. 본 논문에서는 $S_1$, $S_2$를 $Z_p$의 부분집합이면서 밸런스드 부분집합이 되도록 하여 효율성을 개선한다. 결과적으로, 이진 유한체에서의 지수승 연산 속도는 9.1%, 코블리츠 곡선에서의 스칼라 곱셈 연산 속도는 12.1% 빨라진다.

• IMMUNE NETWORK
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• 제11권5호
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• pp.268-280
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• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 춘계종합학술대회
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• pp.264-267
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• 2003

현재 다양한 형태의 B2B 전자 상거래가 진행되고 있으며 전자 상거래에 이용되는 e-Catalog는 무한한 인터넷 공간에서 효과적으로 기업의 상품 및 제품의 특성을 잘 표현할 수 있는 중요한 요소 중 하나이다. 그러나 전자 상거래에 사용되고 있는 e-Catalog 시스템은 명확한 개념과 일관된 구성 요소 등이 없고 서로 상이한 시스템을 구축하고 있으며 이로 인해 e-Catalog 정보를 교환 및 거래에 사용할 수 없는 단점이 있다. 이에 본 논문에서는 이기종 시스템에서 상호 운용성을 증대시키기 위하여 XML을 e-catalog의 표준 문서포맷으로 제안하고, XML schema를 이용하여 통합적으로 상품정보를 처리 할 수 있는 e-Catalog 문서 구조를 정의하였다. 또한 XML로 정의된 e-Catalog 문서를 이용하여 검색, 등록 서비스를 제공하는 e-Catalog 레지스트리 시스템을 설계 및 구현하였다.

• 한국물환경학회지
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• 제39권5호
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• pp.396-412
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• 2023

Species from the mayfly genus Ephemera (Order Ephemeroptera) was assessed for their physical microhabitat suitability (namely E. strigata, E. separigata, and E. orientalis-sachalinensis). Probability distribution models (Exponential, Normal, Lognormal, Logistic, Weibull, Gamma, Beta, and Gumbel) based on the data collected from 23,957 sampling units of 6,787 sites in Korea from 2010 to 2021 were used. Mode and standard deviation calculated from the best-fitting models to species distribution along a water depth gradient were 265 cm and 159 cm in E. orientalis-sachalinensis; 10 cm and 83 cm in E. strigata; 20 cm and 15 cm in E. separigata, respectively. The current velocity gradient was 22 cm/s and 40 cm/s in E. orientalis-sachalinensis; 60 cm/s and 53 cm/s in E. strigata; 82 cm/s and 25 cm/s in E. separigata, respectively. The mean diameter (phi scale) of substrate grains were -3.6 and 2.2 in E. orientalis-sachalinensis; -7.4 and 1.5 in E. strigata; -5.8 and 0.9 in E. separigata, respectively. Habitat suitability range of E. orientalis-sachalinensis was estimated to be 161~369 cm (water depth), 5~44 cm/s (current velocity), -5.2~-2.0 (mean diameter); 3~34 cm (water depth), 36~94 cm/s (current velocity), -8.1~-6.3 (mean diameter) for E. strigata; 12~32 cm (water depth), 63~96 cm/s (current velocity), -6.3~-5.2 (mean diameter) for E. separigata. In relative comparison, E. orientalis-sachalinensis was estimated to be rheophobic, eurybathophilic, and eurypsephophilic; E. strigata to be euryrheophilic, bathophobic, and lithophilic; E. separigata to be stenomesorheophilic, stenobathophobic, stenolithophilic.