• BMB Reports
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• 제42권11호
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• pp.747-751
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• 2009

Transcriptional silencing of subtelomeric genes is associated with telomere length, which is correlated with age. Long and short telomeres in young and old people, respectively, coincide with gene repression and activation in each case. In addition, differential location of genes with respect to telomeres causes telomere position effect. There is very little evidence of the manner in which age-related telomere length affects the expression of specific human subtelomeric genes. We analyzed the relationship between telomere length and gene expression levels in fibroblasts derived from human donors at ages ranging from 0-70 years. We studied three groups of genes located between 100 and 150 kb, 200 and 250 kb, and >300 kb away from telomeres. We found that the chromatin modifier-encoding genes Eu-HMTase1, ZMYND11, and RASA3 were overexpressed in adults. Our results suggest that short telomere length-related overexpression of chromatin modifiers could underlie transcriptional changes contributing to cellular senescence.

• Animal Bioscience
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• 제36권9호
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• pp.1435-1444
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• 2023

Objective: This study was conducted to evaluate Raman spectroscopy technique as a noninvasive tool to predict meat quality traits on Braford longissimus thoracis et lumborum muscle. Methods: Thirty samples of muscle from Braford steers were analyzed by classical meat quality techniques and by Raman spectroscopy with 785 nm laser excitation. Water holding capacity (WHC), intramuscular fat content (IMF), cooking loss (CL), and texture profile analysis recording hardness, cohesiveness, and chewiness were determined, along with fiber diameter and sarcomere length by scanning electron microscopy. Warner-Bratzler shear force (WBSF) analysis was used to differentiate tender and tough meat groups. Results: Higher values of cohesiveness and CL, together with lower values of WHC, IMF, and shorter sarcomere were obtained for tender meat samples than for the tougher ones. Raman spectra analysis allows tender and tough sample differentiation. The correlation between the quality attributes predicted by Raman and the physical measurements resulted in values of R² = 0.69 for hardness and 0,58 for WBSF. Pearson's correlation coefficient of hardness (r = 0.84) and WBSF (r = 0.79) parameters with the phenylalanine Raman signal at 1,003 cm^-1, suggests that the content of this amino acid could explain the differences between samples. Conclusion: Raman spectroscopy with 785 nm laser excitation is a suitable and accurate technique to identify beef with different quality attributes.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7583-7586
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• 2013

Background: Suppressor of cytokine signaling (SOCS)-1 acts as a key regulator of many cytokine signaling pathways and its abnormal expression has been identified in several human malignancies, suggesting potential roles in carcinogenesis. The aim of this study was to investigate any association between the functional SOCS-1 -1478CA>del polymorphism and colorectal cancer (CC) as well as age at onset in a Turkish clinical sample. Materials and Methods: A total of 122 subjects were enrolled in this case-control study (70 CC cases and 52 controls). The SOCS-1 -1478CA>del polymorphism was genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The odds ratio of the del allele for CC relative to the CA allele was not significantly different between the groups (OR=0.71, 95% CI=0.41-1.22, p=0.27). This result did not change after adjustment for age and sex on multivariable regression analysis (OR=0.84, 95% CI=0.59-1.34, p=0.53). When the SOCS-1 -1478CA>del polymorphism was analyzed among CC patients in relation to the age at disease onset, we found no significant differences between subjects with the del/del, CA/del, and CA/CA genotypes. Conclusions: The results of our study did not point towards a major role of the SOCS-1 -1478CA>del polymorphism in the pathogenesis of CC in Turkish subjects.

• Journal of Power Electronics
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• 제11권3호
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• pp.381-388
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• 2011

This article presents the development of a model for SiC power diodes based on the physics of the semiconductor. The model is able to simulate the behavior of the dynamics of the charges in the N- region based on the stored charge inside the SiC power diode, depending on the working regime of the device (turn-on, on-state, and turn-off). The optimal individual calculation of the ambipolar diffusion length for every phase of commutation allows for solving the ambipolar diffusion equation (ADE) using a very simple approach. By means of this methodology development a set of differential equations that models the main physical phenomena associated with the semiconductor power device are obtained. The model is developed in Pspice with acceptable simulation times and without convergence problems during its implementation.

• Structural Engineering and Mechanics
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• 제72권5호
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• pp.585-595
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• 2019

Foundations are a vital part of structures. Over time, the foundations can deteriorate due to unforeseen overloads and/or settlements, resulting in the appearance of cracks in the concrete. These cracks produce changes in the static and dynamic behavior of the affected foundation, which alter its load carrying capacity. In this work, non-destructive techniques of relative simplicity of application are presented for the detection, location, and quantification of damage, using numerical models, solved with the finite element method and Power Series. For this, two types of parameters are used: static (displacement and elastic curvature) and dynamics (natural frequencies). In the static analysis, the damage detection is done by means of a finite elements model representing a beam supported on an elastic foundation with a discrete crack that varies in length and location. With regard to dynamic analysis, the governing equations of the model are presented and a method based on Power Series is used to obtain the solution for a data set, which could be the Winkler coefficient, the location of the crack or the frequency. In order to validate the proposed methodologies, these techniques are applied to data obtained from laboratory tests.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.479-482
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• 2004

The behavior of fatigue delamination in a GLARE(Glass Fiber Reinforced Metal Laminates) under fatigue loading conditions investigated. The behavior of fatigue delamination was examined basing on investigation of the crack and delamination using a SAM (Scanning Acoustic Microscope). The crack and delamination behavior on the relationship among a-N, SAM images and crack length-delamination length were considered. The test results indicated the features of different fatigue delamination and crack growth according to each fiber orientation angle and also obtained to more increase delamination than crack through the relationship between crack length and delamination length in GLARE.

• Asian Pacific Journal of Cancer Prevention
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• 제16권8호
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• pp.3559-3563
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• 2015

The aim of the present study was to evaluate the relative contribution of CYP1A2 isoforms (-3860 G/A, -2467T/delT and -163C/A) in control subjects and breast cancer patients to the metabolism of caffeine in human liver. Restriction fragment length polymorphism analysis of PCR-amplified Fragments (PCR-RFLP) was used for the genotyping of CYP1A2 SNPs and HPLC allowed the phenotyping through the measurement of CYP1A2 activity using the 17X + 13X + 37X/137X urinary metabolite ratio (CMR) and plasma caffeine half life (T1/2). The CYP1A2 -3860A genotype was associated with a decreased risk of breast cancer. In contrast, distributions of the CYP1A2 -2467T/delT or -2467delT/delT and -163A/C or A/A genotypes among breast cancer patients and controls were similar. When the genotype and phenotype relationship was measured by comparing the mean CMR ratios and caffeine half life within the genotype groups between subjects and breast cancer patients, there were no significant differences except for -3860 A, most of them being homozygous for the -3860 G/G SNP and had a significant higher mean CMR ratio and half life than those with -3860 G/A (P=0.02). The results of this preliminary study show a significant association between CP1A2 -3860 G variant and CYP1A2 phenotype which must be confirmed by further large-size case-control studies.

• Journal of Gastric Cancer
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• 제17권3호
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• pp.267-276
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• 2017

Purpose: Different esophagojejunostomy (EJ) reconstruction methods are used after totally laparoscopic total gastrectomy (TLTG), and none is considered a standard technique. This report describes a 2-layer hand-sewn EJ technique during TLTG; we also evaluated postoperative morbidity associated with this technique. Materials and Methods: This retrospective cohort study included all consecutive patients who underwent TLTG for gastric cancer (GC) from 2012 to 2016 at 2 affiliated teaching hospitals. All participating surgeons performed standardized intracorporeal 2-layer hand-sewn EJ. Results: We included 51 patients who underwent TLTG for GC and standardized EJ anastomosis. Twenty-seven (53%) were male, and the median age was 60 (36-87) years. The average operative time was $337{\pm}71minutes$ and intraoperative bleeding was $160{\pm}107mL$. There were no open conversions related to EJ. Postoperative morbidity was observed in 9 (17.0%) patients. There was no postoperative mortality. EJ leakage was observed in 2 patients (3.8%) and 1 patient (1.9%) developed EJ stenosis. Patients with leakage were managed non-operatively and the patient with stenosis required endoscopic dilation. The median length of hospital stay was 8 (6-29) days. Conclusions: Two-layer hand-sewn EJ during TLTG for GC is a feasible and safe technique. This method avoids a laparotomy for reconstruction and the disadvantages associated with laparoscopic introduction of mechanical staplers for EJ, and provides an alternative for alimentary tract reconstruction after TLTG.

• 한국전자파학회논문지
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• 제28권4호
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• pp.318-327
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• 2017

능동위상배열안테나는 정밀한 빔의 형성과 성능 확인을 위해 근접전계 수신시험이 요구된다. 그에 따른 근접전계 수신시험을 통해 모노펄스 기능을 활용하기 위한 보상값을 획득하게 된다. 하지만, 많은 주파수 채널을 사용하는 레이다의 경우 많은 시간과 노력이 요구된다. 효율적인 레이다 개발과 생산을 위해서는 선택적으로 주파수 채널을 선정하고, 그 측정값을 기준으로 측정하지 않은 주파수 채널의 보상값을 보간법을 통해 예측하여 획득할 수 있다. 이와 같은 방법으로 보상값의 위상각 정보를 획득할 경우, 합, 차 채널간의 위상변화량을 확인할 필요성이 있다. 위상의 측정은 랩핑으로 인하여 비선형 현상이 나타날 수 있기 때문이다. 일반적인 레이다의 경우, 합, 차 채널의 전기적 길이가 유사하게 설계되지만, 특정 목적으로 전기적 길이 차이가 발생할 경우, 랩핑 현상으로 인해 보간법의 오류를 발생할 수 있다. 본 논문에서는 전기적 길이 차이에 의해 발생되는 보간법의 오류를 확인하고, 하나의 주파수 선택 방안을 제시한다.

• 대한수학회지
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• 제52권1호
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• pp.209-224
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• 2015

Computing square, cube and n-th roots in general, in finite fields, are important computational problems with significant applications to cryptography. One interesting approach to computational problems is by using polynomial representations. Agou, Del$\acute{e}$eglise and Nicolas proved results concerning the lower bounds for the length of polynomials representing square roots modulo a prime p. We generalize the results by considering n-th roots over finite fields for arbitrary n > 2.