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검색결과 132건 처리시간 0.029초

Tobamovirus Coat Protein CPCg Induces an HR-like Response in Sensitive Tobacco Plants

  • Ehrenfeld, Nicole;Canon, Paola;Stange, Claudia;Medina, Consuelo;Arce-Johnson, Patricio
    • Molecules and Cells
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    • 제19권3호
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    • pp.418-427
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    • 2005
  • When inoculated into sensitive tobacco Xanthi-nn plants, the crucifer and garlic-infecting Tobacco mosaic virus (TMV-Cg) induces local necrotic lesions that resemble those seen in the hypersensitive response (HR) of resistant tobacco plants. However, unlike these, tobacco Xanthi-nn plants do not become resistant to infection and the virus spreads systemically causing a severe disease characterized by necrotic lesions throughout the plant. To identify the viral protein that elicits this necrotic response, we used a set of hybrid viruses constructed by combination of TMV-Cg and the tobacco mosaic virus strain U1 (TMV-U1). In this study we present evidence that the coat protein of TMV-Cg (CPCg) is the elicitor of the necrotic response in tobacco Xanthi-nn plants. Local and systemic necrotic lesions induced by TMV-Cg and by the hybrid U1-CPCg -that carries CPCg in a TMV-U1 context- are characterized by cell death and by the presence of autoflorescent phenolic compounds and $H_2O_2$, just like the HR lesions. In addition, defense-related genes and detoxifying genes are induced in tobacco Xanthi-nn plants after TMV-Cg and U1-CPCg inoculation. We postulate that in our system, CPCg is recognized by sensitive tobacco plants that mount an incomplete defense response. We call this an HR-like since it is not enough to induce plant resistance.

THE EFFECT OF BLOCK RED-BLACK ORDERING ON BLOCK ILU PRECONDITIONER FOR SPARSE MATRICES

  • GUESSOUS N.;SOUHAR O.
    • Journal of applied mathematics & informatics
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    • 제17권1_2_3호
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    • pp.283-296
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    • 2005
  • It is well known that the ordering of the unknowns can have a significant effect on the convergence of a preconditioned iterative method and on its implementation on a parallel computer. To do so, we introduce a block red-black coloring to increase the degree of parallelism in the application of the block ILU preconditioner for solving sparse matrices, arising from convection-diffusion equations discretized using the finite difference scheme (five-point operator). We study the preconditioned PGMRES iterative method for solving these linear systems.

The effects of non-thermal plasma and conventional treatments on the bond strength of fiber posts to resin cement

  • do Prado, Maira;da Silva, Eduardo Moreira;Marques, Juliana das Neves;Gonzalez, Caroline Brum;Simao, Renata Antoun
    • Restorative Dentistry and Endodontics
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    • 제42권2호
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    • pp.125-133
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    • 2017
  • Objectives: This study compared the effect of hexamethyldisiloxane (HMDSO) and ammonia ($NH_3$) plasmas on the bond strength of resin cement to fiber posts with conventional treatments. Materials and Methods: Sixty-five fiber posts were divided into 5 groups: Control (no surface treatment); $H_2O_2$ (24% hydrogen peroxide for 1 min); Blasting (blasting with aluminum oxide for 30 sec); $NH_3$ ($NH_3$ plasma treatment for 3 min); HMDSO (HMDSO plasma treatment for 15 min). After the treatments, the Ambar adhesive (FGM Dental Products) was applied to the post surface (n = 10). The fiber post was inserted into a silicon matrix that was filled with the conventional resin cement Allcem Core (FGM). Afterwards, the post/cement specimens were cut into discs and subjected to a push-out bond strength (POBS) test. Additionally, 3 posts in each group were evaluated using scanning electron microscopy. The POBS data were analyzed by one-way analysis of variance and the Tukey's honest significant difference post hoc test (${\alpha}=0.05$). Results: The Blasting and $NH_3$ groups showed the highest POBS values. The HMDSO group showed intermediate POBS values, whereas the Control and $H_2O_2$ groups showed the lowest POBS values. Conclusion: Blasting and $NH_3$ plasma treatments were associated with stronger bonding of the conventional resin cement Allcem to fiber posts, in a procedure in which the Ambar adhesive was used.

CMnAl TRIP Steel Surface Modification During CGL Processing

  • Gong, Y.F.;Lee, Y.R.;Kim,, Han-S.;Cooman, B.C.De
    • Corrosion Science and Technology
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    • 제9권2호
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    • pp.81-86
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    • 2010
  • The mechanisms of selective oxidation of intercritically annealed CMnAl TRIP steels in a Continuous Galvanizing Line (GCL) were studied by cross-sectional observation of the surface and sub-surface regions by means of High Resolution Transmission Electron Microscopy (HR-TEM). The selective oxidation and nitriding of an intercritically annealed CMnAl TRIP steel in a controlled dew point 10%$H_2+N_2$ atmosphere resulted in the formation of c-xMnO.$MnO_2$ (1${\leq}$x<3) and c-xMnO.$Al_2O_3$ ($x{\geq}1$) particles on the steel surface. Single crystal c-xMnO.$SiO_2$ ($2{\leq}x{\leq}4$) oxide particles were also observed on the surface. A thin film of crystalline c-xMnO.$SiO_2$ (2${\leq}$x<3) and c-xMnO.$Al_2O_3$ ($x{\geq}1$) was present between these particles. In the sub-surface region, internal oxidation, nitriding and intermetallic compound formation were observed. In the first region, large crystalline c-xMnO.$SiO_2$ ($1{\geq}x{\geq}2$) and c-xMnO.$Al_2O_3$ ($x{\geq}1$) oxides particles were present. In the second region, c-AlN particles were observed, and in a third region, small $MnAl_x$ (x>1) intermetallic compound particles were observed.

수렴성전자회절에 의한 n-형 세라믹 초전도체 $Nd_{2-x}Ce_xCuO_{4-\upsilon}$의 결정구조 전이 연구 (Inestigation on the Structural Transition of n-type Ceramic Superconductor, $Nd_{2-x}Ce_xCuO_{4-\upsilon}$ System of CBED)

  • 김정식;유광수
    • 한국세라믹학회지
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    • 제34권2호
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    • pp.139-144
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    • 1997
  • 결정학적으로 T' 구조인 Nd2-xCexCuO4-$\delta$ 회토류 구리산화물 초전도체는 지금까지 매우 복잡한 미세구조를 지닌 것으로써 잘 알려져 있다. 이 구리산화물 초전도체가 초전도 특성을 띠려면, Nd3+ 이온이 Ce4+ 이온에 의해 소량 치환되고 동시에 산소환원이 필요하다. 본 연구에서는 투과전자현미경의 수렴성 전자회절(CBED)패턴을 이용하여 Nd2-xCexCuO4-$\delta$ 의 Ce 미량 첨가와 미량의 산소환원에 따른 결정구조의 전이 발생 여부에 관하여 고찰하였다. 세 가지의 서로 다른 시편 Nd2CuO3,Nd1.85Ce0.15CuO4 와Nd1.85Ce0.15CuO3.965을 고상소결에 의하여 만들어 이들 각각 시편에 대한 CBED패턴을 주사투과전자현미경(STEM)으로 관찰하였다. 그리고 실험에 의하여 얻은 이들 HOLZ선들과 컴퓨터 계산에 의해 그려진 이론적인 HOLZ선들과 비교하는 방법에 의하여 Nd1.85Ce0.15CuO4의 격자상수를 구하였다.

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깍두기 숙성 중 N-Nitrosamine의 생성 (The Formation of N-Nitrosamine during Fermentation of Kakdugi)

  • 성낙주;구경숙;신정혜;정미자;이수정
    • 한국식품영양과학회지
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    • 제28권1호
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    • pp.33-39
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    • 1999
  • Traditional kakdugi prepared from salted radish by the addition of fermented shrimp or anchovy sauce along with seasonings and spices, e.g., red pepper powder, garlic or ginger, which has been examined for the formation of volatile nitrosamine(NA) during its fermentation at 5oC and 17oC. Changes of acidity were increased; while, those of ascorbic acid and pH were continually de creased and besides concentrations of salt were scarcely observed during the fermentation of kakdugi at 5oC and 17oC. High initial levels of nitrate in radish followed by a gradual decreased in nitrate concentration, along with increasing levels of nitrite, during its fermentation. During the fermentation of kakdugi, NA such as N nitrosodimethylamine(NDMA) and N nitrosodiethylamine(NDEA) was identified; these NA in kakdugi that had been prepared with shrimp sauce contained in the higher levels than kimchi that had been prepared with anchovy sauce, and both NDMA and NDEA were detected higher levels in the fermented sample at 17oC than in the fermented sample at 5oC. In kakdugi, NDMA and NDEA levels were continually increased during its fermentation; its levels after 35 days fermentation at 17oC were 0.5~16.7 and 0~5.2 g/kg in added shrimp sauce sample, 0. 5~8.7 and 0~4.7 g/kg in added anchovy sauce sample and 0.4~4.8 and 0~2.4 g/kg in control, respectively. The occurrence of NA such as NDMA and NDEA appeared to arise from the fermented sauce and radish used in preparation of the kimchi.

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Economic construction management of composite beam using the head stud shear connector with encased cold-formed steel built-up fix beam via efficient computer simulation

  • Yin, Jinzhao;Tong, Huizhi;Gholizadeh, Morteza;Zandi, Yousef;Selmi, Abdellatif;Roco-Videla, Angel;Issakhov, Alibek
    • Advances in concrete construction
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    • 제11권5호
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    • pp.429-445
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    • 2021
  • With regard to economic efficiency, composite fix beams are widely used to pass longitudinal shear forces across the interface. The current knowledge of the composite beam load-slip activity and shear capability are restricted to data from measurements of push-off. Modelling and analysis of the composite beams based on Euro-code 4 regarding to shear, bending, and deflection under differing loads were carried out using Finite Element through an efficient computer simulation and the final loading and sections capacity based on the failure modes was analysed. In bending, the section potential was increased by an improvement of the strength in both steel and concrete, but the flexural and compressive resistance growth is very weak (3.2% 3.1% and 3.0%), while the strength of the concrete has increased respectively from 25 N/mm2 to 30, 35, and 40 N/mm2 compared to the increment of steel strength by 27% and 21% when it was raised from 275 to 355 and 460 N/mm2, respectively. It was found that the final flexural load capacity of fix beams was declined with increase in the fix beam span for both three steel strength. The shear capacity of sections was remained unchanged at constant steel strength and different length, but raised with final yield strength increment of steel sections by 29%, and 67% when it was raised from 275 N/mm2 to 355 N/mm2 and 460 N/mm2, respectively.

Activation Energy for the Decapsulation of Small Molecules from A-Type Zeolites

  • 김정섭;황계정;홍석봉;노경태
    • Bulletin of the Korean Chemical Society
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    • 제18권3호
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    • pp.280-286
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    • 1997
  • Potential energy function sets for some ion-exchanged A-type zeolites, K-A and Rb11Na1-A, were determined by introducing the X-ray crystal structures as constraints. The potential functions reproduced well the X-ray crystal structures of the monovalent ion-exchanged zeolites. The activation energies for the en- or de-capsulation of small molecules (H2, O2, N2, and CH4) and inert gases from the α-cage of model zeolites (Na-A, K-A, Rb11Na1-A, and Cs3Na9-A) were obtained by the molecular mechanical calculations. The calculated activation energies agreed well with experimental results.

해양 패류 렉틴성분 연구 (V) - 반지락조개의 렉틴성분 분리정제에 관한 연구 (Studies on Lectins From Marine Shells (V) - Isolation and Purification of Letin from Tapes philippinarum.)

  • 정시련;김장환;전경희
    • 약학회지
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    • 제31권2호
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    • pp.52-59
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    • 1987
  • The result of the screening of lectins on 28 species of marine shell fishes(mollusks) showed that 10 species (Tapes philippinarum, Cyclosunetta menstrualis, Neptunea arthritica cumingii, Omphalius pfeifferi carpenteri, Chlorostoma argyrostoma turbinatum, Chiorostoma argyrostoma lischkei, Semisulcosira corea, Neptunea polycosta, Babylonica japonica, Noverita didyma) were present hemagglutinating properties to human A, B, and O group, and animal blood erythrocytes. A new lectin from Tapes philippinarum. was isolated and partially characterized. The lectin was purified by (NH$_4$)$_2$SO$_4$ precipitation and ion exchange chromatography on DE 53 column. Six fractions were obtained from DE 53 column by salt gradient elution but only 0.3M, and 0.4M NaCl fraction had strong lectin activity. On its 0.3M NaCl fraction, purity was identified by polyacrylamide gel electrophoresis. This lectin was inhibited by N-acetyl-D-galactosamine. It seems that two kinds of lectin react as antigen by immunochemical studies.

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KINEMATICAL PROPERTIES OF PLANETARY NEBULAE WITH WR-TYPE NUCLEI

  • DANEHKAR, ASHKBIZ;STEFFEN, WOLFGANG;PARKER, QUENTIN A.
    • 천문학논총
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    • 제30권2호
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    • pp.163-167
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    • 2015
  • We have carried out integral field unit (IFU) spectroscopy of $H{\alpha}$, [$N{\small{II}}$] and [$O{\small{III}}$] emission lines for a sample of Galactic planetary nebulae (PNe) with Wolf-Rayet (WR) stars and weak emission-line stars (wels). Comparing their spatially-resolved kinematic observations with morpho-kinematic models allowed us to disentangle their three-dimensional gaseous structures. Our results indicate that these PNe have axisymmetric morphologies, either bipolar or elliptical. In many cases the associated kinematic maps for the PNe around hot central stars also reveal the presence of so-called fast low-ionization emission regions.