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The Pathogenicity and Biochemical Characteristics of Vibrio harveyi Isolated from the Pacific Abalone, Haliotis discus hannai (양식 전복(Haliotis discus hannai)으로부터 분리된 Vibrio harveyi의 생화학적 특성 및 병원성)

  • Kim, Jin-Do;Kim, Myoung-Sug;Won, Kyung-Mi;Do, Jeong-Wan;Lee, Deok Chan;Jung, Sung Hee;Jin, Se Yoon;Lee, Nam-Sil;Cho, Miyoung
    • Korean Journal of Environmental Biology
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    • v.35 no.4
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    • pp.670-676
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    • 2017
  • Recently, mass mortality of the young abalone Haliotis discus hannai has occurred in commercial seed production farms in Korea. The mortality rate was above 50% of the total cultured organisms in the farm, and the shell length of the moribund organisms was about 3cm. The mortal phenomenon was that the young abalones were weakly scattered on the bottom of the pond from the attachment matrix, or that they could not be moved back to their normal positions. The diseased farmed Pacific abalone had abdominal edema. From the edema in the moribund individuals, three bacterial strains were isolated and all the strains were identified as Vibrio harveyi. These strains were compared with thirty six strains isolated from the fish. The results was that the Vibrio harveyi from the fish were sorted into genogroup A or B; however, the three strains of the diseased farmed Pacific abalone were sorted into genogroup A and the new genogroup C. The identical mortality and pathological symptoms of the naturally infected organisms were reproduced by artificial infection with WA AG-1 and WA CS-5 strains. The $LD_{50}$ of WA AG-1 and WA CS-5 were each $1.0{\times}10^3cfu\;animal^{-1}$ and $1.7{\times}10^4cfu\;animal^{-1}$.

Final Results about Science issues in CPM-15 2nd meeting

  • Chung, HyunSoo;Moon, Jun-Cheol;Yu, Dai-Hyuk;Je, Do-Heung;Jo, Jung-Hyun;Roh, Duk-Gyoo;Oh, Se-Jin;Sohn, Bong-Won;Lee, SangSung;Kim, Hyo-Ryung
    • The Bulletin of The Korean Astronomical Society
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    • v.40 no.1
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    • pp.83.1-83.1
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    • 2015
  • 세계전파통신회의 (WRC; World Radiocommunication Conference)회의는 국제전기통신연합 (ITU)에서 규정하는 국제 전파법 제개정을 위해, 3-4년 간격으로 개최되는 전파통신 관련 최고회의이다. 2015년 11월 2일-27일에 개최되는 WRC-15 본회의에서 다루는 28개 의제에 대해서는 의제별 주파수대역별로 공유/양립성/보호 연구결과가 제시되어야 한다. 따라서 2015년 3월 23일-4월 2일에는 스위스 제네바에서 190여개 ITU회원국의 국가대표 1,000여명이 모여서 의제별 최종연구결과보고서를 작성하며(CPM-15 2차회의, Conference Preparatory Meeting), 그 결과는 11월의 WRC회의에서 중요한 잣대 역할을 하게 된다. 동 회의에서 다루는 의제 가운데, 과학업무 연구반에서 다루는 주요 의제들은 다음과 같다. 1) 7145-7250 MHz 대역의 지구탐사위성(지구대우주)업무의 1순위 분배연구, 2) 8,700~10,500MHz대역의 지구탐사위성업무의 신규 SAR용대역(연속 1.2GHz) 분배방안 연구, 3) 우주선 근거리통신용 410-420MHz 대역 규제 철폐 관련 검토, 4) 윤초 삭제 또는 개정 방안 연구가 있다. 그리고 모바일 광대역 응용 실현을 위한 이동통신업무 추가 분배 및 IMT 추가 지정 연구, 나노 위성 및 피코 위성 규제 관련 연구들이 있다. 따라서 본 발표에서는 3월에 개최된 CPM-15 2차회의의 과학업무 의제 관련 최종결과를 소개하고, WRC-15회의에 대비하여 국내 전파천문업무 보호를 위해 준비가 필요한 주요 이슈에 대해 소개를 하고자 한다.

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A Study of the Properties of Magnetic Particles in Medicinal Ointments (의학용 연고제에 포함된 자성물질 입자들의 성질에 대한 연구)

  • Do, Tae-Sung;Nam, Hyo-Duk;Park, Se-Gon;Hwang, Hyeun-Gook;Hwang, Yong-Soon;Kim, Eng-Chan;Park, Eun-Jung;V. I. Nikolaev;A. V. Bykov
    • Progress in Medical Physics
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    • v.8 no.1
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    • pp.69-79
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    • 1997
  • Stability of the properties of magnetite particles in novel medicinal magnetic ointments of multipurpose application has been studied by M ssbauer spectroscopy. It has been found that the comparative analysis of the results obtained by model fitting of $^{57}$ Fe nuclei spectra with those known for the system Fe$_3$O$_4$-${\gamma}$-Fe$_2$O$_3$ allows to identify the phase composition of the particles. It is noted that this composition, as well as that of the initial pure component in the form of a highly dispersed fraction (~100$\AA$), differs noticeably from the stoichiometric one. From the magnetic hyperfine field despite small particle sizes, the particles exhibit no superparamagnetism (in the temperature range from 95 to 300K). Radiative sterilization of the ointments has no effect on the magnetic component composition.

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PKC inhibitors RO 31-8220 and Gö 6983 enhance epinephrine-induced platelet aggregation in catecholamine hypo-responsive platelets by enhancing Akt phosphorylation

  • Kim, Sun-Young;Kim, Se-Woon;Kim, Jeong-Mi;Jho, Eek-Hoon;Park, Seon-Yang;Oh, Do-Yeun;Yun-Choi, Hye-Sook
    • BMB Reports
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    • v.44 no.2
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    • pp.140-145
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    • 2011
  • Impaired responsiveness of platelets to epinephrine (epi) and other catecholamines (CA) has been reported in approximately 20% of the healthy Korean and Japanese populations. In the present study, platelet aggregation induced by epi was potentiated by RO 31-8220 (RO) or G$\ddot{o}$ 6983 (G$\ddot{o}$). Phosphorylated Akt (p-Akt) was very low in epi-stimulated PRP from CA-hypo- responders (CA-HY), whereas it was detected in those from CA-good responders (CA-GR). RO and G$\ddot{o}$ increased p-Akt, one of the major downstream effectors of phosphoinositol-3 kinase (PI3K), in epi-stimulated PRP from both groups. Wortmannin, a PI3K inhibitor, attenuated the RO or G$\ddot{o}$-induced potentiation of p-Akt in epi-stimulated PRP, suggesting positive effects for RO and G$\ddot{o}$ on PI3K. $TXA_2$ formation was increased by the addition of either RO or G$\ddot{o}$ in epi-stimulated platelets. The present data also suggest that impaired Akt phosphorylation may be responsible for epinephrine hypo-responsiveness of platelets.

Reproductive Cycle of the Bay Scallop, ArEopecten irradians Transplanted from China (중국에서 이식한 해만가리비, Argopecten irradians의 생식주기)

  • Oh Bong Se;Jung Choon Goo;Kim Sung Yeon;Kim Sook Yang;Chung Ee Yung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.3
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    • pp.201-206
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    • 2002
  • Reproductive cycle of the bay scallop, Argopecten irradians, transplanted from China on 16 August 1996, was investigated monthly until August 1997 in the Deukyang Bay, Jangheung-gun, Jeollanam-do, Korea. Sexuality of this species is hermaphrodite and oviparous, Monthly changes in the soft part weight index and adductor muscle weight (g) began to increase from October and reached the maximum in November, and then the values gradually decreased from December to August, The gonadosomatic index (GI) began to increase in March and reached the maximum in April. Thereafter, the values gradually decreased from May to December, The reproductive cycle of this species, transplanted in the south coast of Korea could be divided into 5 successive stages; early active (October to February), late active (November to May), ripe (February to May), spawning (May to June) with a peak in May, and spent/inactive stage (June to August).

Air Environmental Characteristics of a Greenway Park in Gwangju (푸른길 공원의 대기 환경 특성에 관한 연구)

  • Min, Kyoung-Woo;Lee, Kyoung-Soek;Park, Ok-Hyun;Yoon, Kwan-Ju;Kim, Do-Sool;Park, Se-Il;Jeung, Won-Sam;Lee, Dae-Hang;Cho, Young-Gwan
    • Journal of Environmental Health Sciences
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    • v.41 no.3
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    • pp.171-181
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    • 2015
  • Objectives: This study aimed to survey the characteristics of air quality and meteorological conditions in a greenway park. Methods: We measured meteorological and health related factors, including noise, particulate matter ($PM_{10}$) and selected gaseous air pollutants at three locations in a greenway park and on a general roadside as comparison. The measurements were repeated four times from April to October 2014. Results: The average air temperature in the greenway park was $20.7^{\circ}C$ which was $1-2^{\circ}C$ lower than on the general roadside. The average $PM_{10}$ concentration in the greenway park was $85.0{\mu}g/m^3$, a level 2-3 times lower than that at the roadside. The noise level at the greenway site was 4.4 dB(A)- 23.0 dB(A) lower than at the roadside. The average CO, $CO_2$, $SO_2$ and NOx concentrations in the greenway park were lower than at the roadside. The average phytoncide and anions concentrations in the greenway park were higher than at the roadside. Conclusions: The urban forest of the greenway park may have some impact on air quality and meteorological conditions.

The Transmission Electron Microscopic Study on the Alteration of Filtration Barrier in Aged Rat Kidney (흰쥐 콩팥여과관문의 노화 변화에 관한 투과전자현미경적 연구)

  • Lee, Se-Jung;Lim, Hyoung-Soo;Lim, Do-Seon;Hwang, Douk-Ho
    • Applied Microscopy
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    • v.38 no.2
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    • pp.107-115
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    • 2008
  • The filtration barrier of kidney consists of endothelial cell, glomerular capillary, glomerular basement membrane, mesangial matrix, and podocyte. In aged rats, the morphological changes were shown in various parts, including the glomerulus. These changes were thickening of basement membrane and mesangial matrix, crescent formation of glomerular capillary, deformity of foot processes, glomerular sclerosis and obsolescence. But these glomerular morphologies are partial images or few serial images analysis. In this study, we examined the morphological alteration of glomerulus in the young and aged rats by light microscopy, transmission electron microscopy and three dimensional reconstruction. We were found in aged rat glomerulus, expansion of urinary space and mesangial matrix, thickening and degrading of glomerular basement membrane, decreasing in podocyte foot processes, fragmentation of podocytic nucleus membrane. These observations indicate that may provide useful data for investigating the pathogenesis of age-related dysfunction of kidney.

Performance Improvement for Visible Light Communications Using Pre-Equalizer and Optical Design (전치 등화기와 광학설계를 이용한 가시광통신 전송 용량 및 거리 향상 연구)

  • Kwon, Do-Hoon;Yang, Se-Hoon;Kim, Hyun-Seung;Son, Yong-Hwan;Han, Sang-Kook
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39C no.6
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    • pp.476-481
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    • 2014
  • In this paper, we design the pre-equalizer of transmitter circuit in order to enhancement modulation bandwidth of white LED which is light source of VLC (Visible Light Communication). Also, we eliminate yellow light component by optical filtering which mitigate frequency response of white LED. Power loss by optical filtering is overcome by using convex lens. By applying proposed system, 3 dB bandwidth deciding modulation bandwidth of white LED increases from 3 MHz to more than 25 MHz and the transmission distance increases by optical design which secure additional signal power. We optically modulate NRZ-OOK signal to LED and receive light signal using APD. We analyze received data using CSA and RFSA. As a result, we experimently demonstrate the possibility that transmits NRZ-OOK signal up to 30 Mbps in 4.5 m, 50 Mbps in 1.5 m through the pre-equalizer and optical design.

CoMFA Analysis on Inhibitory Effect of $3{\beta}$-Hydroxy-12-oleanen-28-oic Acid Analogues on PTP-1B Activity and Prediction of Active Compounds ($3{\beta}$-Hydroxy-12-oleanen-28-oic Acid 유도체들의 PTP-1B 저해활성에 대한 CoMFA 분석과 활성 분자들의 예측)

  • Kim, Sang-Jin;Kim, Se-Gon;Sung, Nack-Do
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.34 no.2
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    • pp.109-115
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    • 2008
  • The comparative molecular field analyses(CoMFA) models between the substituents with changing groups($R_1{\sim}R_4$) of $3{\beta}$-hydroxy-12-oleanen-28-oic acid derivatives as substrate molecule and their inhibitory activities($pI_{50}$) against protein tyrosine phosphatase(PTP)-1B were derived and discussed quantitatively. The optimized CoMFA F1 model have best predictability and fitness($r^2_{cv.}=0.654$ and $r^2_{ncv.}=0.995$). The order of contribution ratio (%) with CoMFA fields on the inhibitory activities was a steric field(53.0%), electrostatic field(36.2%) and hydrophobic field(10.8%). From the analytical results of CoMFA contour maps, the inhibitory activities were dependent on the R4 group in substrate molecules. Particularly, the new active compounds(P1 & P2) with the inhibitory activity against melanin synthesis were expected.

Growth and characterization of molecular beam epitaxy grown GaN thin films using single source precursor with ammonia

  • Chandrasekar, P.V.;Lim, Hyun-Chul;Chang, Dong-Mi;Ahn, Se-Yong;Kim, Chang-Gyoun;Kim, Do-Jin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.174-174
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    • 2010
  • Gallium Nitride(GaN) attracts great attention due to their wide band gap energy (3.4eV), high thermal stability to the solid state lighting devices like LED, Laser diode, UV photo detector, spintronic devices, solar cells, sensors etc. Recently, researchers are interested in synthesis of polycrystalline and amorphous GaN which has also attracted towards optoelectronic device applications significantly. One of the alternatives to deposit GaN at low temperature is to use Single Source Molecular Percursor (SSP) which provides preformed Ga-N bonding. Moreover, our group succeeds in hybridization of SSP synthesized GaN with Single wall carbon nanotube which could be applicable in field emitting devices, hybrid LEDs and sensors. In this work, the GaN thin films were deposited on c-axis oriented sapphire substrate by MBE (Molecular Beam Epitaxy) using novel single source precursor of dimethyl gallium azido-tert-butylamine($Me_2Ga(N_3)NH_2C(CH_3)_3$) with additional source of ammonia. The surface morphology, structural and optical properties of GaN thin films were analyzed for the deposition in the temperature range of $600^{\circ}C$ to $750^{\circ}C$. Electrical properties of deposited thin films were carried out by four point probe technique and home made Hall effect measurement. The effect of ammonia on the crystallinity, microstructure and optical properties of as-deposited thin films are discussed briefly. The crystalline quality of GaN thin film was improved with substrate temperature as indicated by XRD rocking curve measurement. Photoluminescence measurement shows broad emission around 350nm-650nm which could be related to impurities or defects.

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