• Title/Summary/Keyword: $Chlorophyll\-\

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LHCP phosphorylation and Chlorophyll-Fluorescence Quenching of PSII in Ginseng Thylakoid Members (인삼 틸라코이드에서 광계II의 LHCP 인산화와 형광 Quenching)

  • 양덕조;김명원
    • Journal of Ginseng Research
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    • v.16 no.2
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    • pp.124-128
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    • 1992
  • Vsing the phosphorylated thylakoid membrane induced by 5-35kLux of light intensities, we investigated the chlorophyll nuorescence quenching of PSII and the phosphorylation of LHCPII i in relation to the chlorophyll-bleaching of Panax ginseng C.A. Meyer. In the Presence of DCMU, both of the fluorescence yield of non-phosphorylated thylakoid and the rate of fluorescence quencing dependent on the Phosphorylation were high p. ginseng more then Glyine max L. And at the high light F intensity (above 25 kLux) the fluorescence quenching rate of p. ginseng compared with that of G. max reached nearly to 2 times. The LHCPII of P. ginseng was composed of 3 major Polypeptides (24.5, 26 and 27kD) and 3 minor polypeptides (24, 25.3 and 28.3kD) in the region of 24-29kD and differed, from G. max in both of the number and quantity of polypeptides. Among these polypeptides, the phosphorylated polypeptide dependent on the light intensity was 24kD in P. ginseng.

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Content of Phycocyanins and Growth of Spirulina platensis with Culture Conditions (배양 조건에 따른 Spirulina piatensis의 성장 및 phycocyanin 함량 변화)

  • JOO Dong Sik;JUNG Chung Kuk;LEE Chang Ho;CHO Soon Yeong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.475-481
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    • 2000
  • The growth of the microalgal Spiulina platensis in a batch photobioreactor had been studied to determine the influence of temperature, light intensity, and culture medium on the growth and c-phycocyanin content of the biomass. The most favorable conditions for high biomass and c-phycocyanin production were as follows: light intensity of 3500 lux, temperature of $35^{\circ}C,\;NaHCO_3\;of\;1.0{\%}$ for pH control, $0.2{\~}0.3{\%}\;Na_2CO_3$ for carbon source, and $0.2{\~}0.3{\%}\;NaCO_3$ for nitrogen source. The c-phycocyanin and chlorophyll content on most favorable condition were about $11{\%},\;1.0{\%}$, respectively.

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Effects of Magnesium Deficiency on Induction of Activity of Antioxidative Enzymes (Magnesium 결핍이 항산화효소의 활성유도에 미치는 영향)

  • Kim, B.C.;Lee, J.J.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.431-438
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    • 1997
  • The influence of deficient(7${\mu}M$) and suffcient(1000${\mu}M$) magnesium(Mg) supply on the content of mg, chlorophyll, protein and the activity of superoxide dismutase(SOD), scavengers of superoxide radical($O_2$), and ascorbate peroxidase(AP), $H_2O_2$scavenging enzyme, and glutathione redutase(GR) were studied in pumpkin(Cudurbita moscata $D_{UCHESNE}$) plants over a 11-day period. Over the 11 days Period of growth in nutrient solution with sufficient and deficient Mg supply, the contents of Mg, chlorophyll protein and the activities of the antioxidative enzymes remained more or less constant in Mg-sufficient leaves. In Mg-deficient leaves, the contents of Mg, chlorophyll and protein was seriously decreased with time, however the activities of SOD, AP and GR highly enhanced compared to those of Mg-sufficient. The results indicated the stimulative effect of Mg deficiency on toxic oxygen species and scavenging enzymes in plants.

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Evaluating Pre-silicon Treatment to Alleviate Drought Stress and Increases Antioxidative Activity in Zoysia japonica

  • Bae, Eun-Ji;Han, Jeong-Ji;Choi, Su-Min;Lee, Kwang-Soo;Park, Yong-Bae
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.360-367
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    • 2015
  • This study was performed to determine the effects of silicon on zoysiagrass after the application of drought stress. The daily amount of water or scilicon solution was 150 ml per a pot. For 14 days, plants were treated with 0.1 and 1.0 mM silicon (Si) and with distilled water for control and the drought only-treatment. Afterward, the plants in Si and drought treatment were exposed to a 21-day under drought stress condition but the plants in control received water. The results indicated that the growth and the moisture and chlorophyll contents decreased in the drought only-treatment and 0.1 mM Si compared to the control. However, 1.0 mM Si showed an increase in the growth with a significant increase of water and chlorophyll contents. The MDA and $H_2O_2$ concentrations and electrolyte leakage decreased, while the radical scavenging capacity increased in 1.0 mM Si. 1.0 mM Si showed little to no differences in the growth and no differences in water and chlorophyll contents, electrolyte leakage, MDA and $H_2O_2$ concentrations and antioxidant capacity compared to the control. These results suggested that application of silicon is useful for drought tolerance improvement of zoysiagrass under drought that is occurring in turf fields.

Study of Methylglyoxal and Phosphorus Stress on Algae (조류의 Methylglyoxal과 인 Stress 연구)

  • 이기태
    • Environmental Analysis Health and Toxicology
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    • v.13 no.3_4
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    • pp.133-142
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    • 1998
  • Effects of phosphorous (P) and methylglyoxal (MG) on the cell number, dry weight, chlorophyll content, photosynthetic and respiratory rate, phosphate uptake and protein content of green algae (Scenedesrnus obliquus) were studied. The algal cell number from the medium treated with 0.5-1.0 mM of MG at 1/2 P or 1/4 P concentration was significantly lower than those of algae treated :with full strength of phosphrous in medium. The inhibitory effect of MG on algal cell division was enhenced at low concentration of phosphorous in medium. At the beginning of logrithmic phase of algal growth, the mean dry weight of algae from the medium without MG-treatment in 1/2 P media was significantly higher than that of algae treated with MG. After logrithmic phase of growth cycle, the mean dry weight of algae from the medium with 1.0 mM of MG-treatment in 1/4 P media was significantly lower than that of algae treated with or without MG. At logrithmic phase of algal growth, there were significant differences in the chlorophyll content among all groups of tested algae with various concentrations of P and MG. At 15 days after inoculation, the mean chlorophyll content per algal cell from the media without MG-treatment in 1/2P was significantly higher than that of other cells from MG-treated media. The adverse effect of MG at concentration of 0.5-1.0mM in 1/2 and 1/4 P media on photosynthetic rate was observed. The mean photosynthetic rate of algal cell without P and MG treatment at 15 days after inoculation was significantly higher than that of MGtreated algae. After logarithmic phase, the algal cell treated with 0.5mM of MG with full strength of phosphorous showed significantly high respiratory rate than that of other cell groups. There were significant differences in mean phosphate uptake rate among all groups of Scenedesmus obliquus at logarithmic phase. At 12 days after inoculation, phosphate uptake rate per each algal cell from the basic media without MG and P treatment was rapidly reduced which shows early introduction to stationary phase.

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Properties of Chemical Components of Camellia japonica L. loaves According to Picking Time (동백잎의 채취시기에 따른 화학적 성분 특성)

  • Kim, Bong-Sun;Choi, Ok-Ja;Shim, Ki-Hoon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.5
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    • pp.681-686
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    • 2005
  • The purpose of this study was to analyze the chemical component of Camellia japonica L. according to picking time. Leaves of Camellia japonica L. were picked in April and May,2003. Free sugars (fructose, glucose and sucrose) and organic acids (citric acid, tartaric acid, succinic acid, acetic acid) were present in the Camellia japonica L. leaf. The contents of total free sugars and organic acids increased as picking time was delayed. The major components of free amino acids were aspartic acid, glutamic acid and histidine, and those of total amino acids were histidine and alanine. The contents of total free amino acids and total amino acids were decreased as picking time was delayed, while the ratio of essential amino acids to the total amino acids increased. The amount of minerals (P, Ca, K, Na and Fe), chlorophyll and total polyphenol increased as picking time was delayed.

Verification on Cold-Tolerance of Some Fruit Trees as Species for Urban Greening Plants

  • Lee, Jin-Hee;Oh, Hee-Young;Kwon, O-Man
    • Journal of Environmental Science International
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    • v.26 no.10
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    • pp.1155-1166
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    • 2017
  • This study selected commonly known species of fruit trees, and re-selected the species that endure the stress of extreme cold weather and physiologically restore themselves to the previous state until the following year. Then we could go ahead to propose the species that were appropriate as urban greening plants in weather condition of any part of the country. To do this, we conducted an experiments for six species of fruit trees based on the preference of the general public and recommendation of the experts; Morus alba (English name: mulberries), Diospyros kaki (English name: Persimmon), Prunus persia (English name: Peach), Elaeagnus umbellata var. coreana (English name: Korean Autumn Olive), Malus domestica 'Alps Otome' (English name: Alps Otome), and Prunus mume (English name: Blue Plum). The experiment verifies whether the trees survive without any stress from the cold weather under the national climate conditions (one in the suburbs of Seoul: Yongin city, one in the central Chungcheong region: Daejeon city, and in the southern Gyeongsang region: Jinju city in Korea). The experiment lasted for a year from August 2016 to August 2017. The levels of electrolytic efflux, chlorophyll content, plant height, fresh weight, and dry weight were measured four times (on August of 2016, January, February, and August of 2017) for each tree planted bare ground outdoors. Results showed that Diospyros kaki, Prunus persia, and Malus domestica 'Alps Otome' were proven durable and resistant to winters of all three areas (one in the suburbs of Seoul: Yongin city, one in the central Chungcheong region: Daejeon city, and in the southern Gyeongsang region: Jinju city in Korea). Especially, the increase of chlorophyll content and the reduction of electrolytic efflux were noticeable in Prunus persia than in the other two species, proving itself as the most cold-tolerant among the six species used in the experiment. In addition, interpreting from the physiological restoration data of one-year span before and after getting through winterer, Prunus persia was proven to be the most cold-tolerant species.

A Study on Green Algae Monitoring in Watershed Using Fixed Wing UAV (고정익 무인비행기를 이용한 수계 내 녹조 모니터링 연구)

  • Park, Jung-Il;Choi, Seung-Young;Park, Min-Ho
    • Journal of the Korean Institute of Intelligent Systems
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    • v.27 no.2
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    • pp.164-169
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    • 2017
  • The primary purpose of this study is to determine NDVI analysis methodologies for green algae monitoring system. A fixed wing UAV integrated with multi-spectral sensor has been adopted to capture the images along the watershed in Gumgang River. The study area was near the Baekje water reservoir and the images was captured on July 2016. Pix4D Mapper Pro was used to process the captured images. Through the comparison actual chlorophyll measurement values with NDVI output image, empirical formula was suggested and geo-locational conversion was carried out. As a result of this study chlorophyll image set applied to actual measurement values was able to extracted. For the efficient management of green algae, its monitoring and prevention in terms of disaster management, gathering chlorophyll information using UAV is very beneficial.

Influence of Benomyl on Photosynthetic Capacity in Soybean Leaves

  • Roh, Kwang-Soo;Oh, Mi-Jung;Song, Seung-Dal;Chung, Hwa-Sook;Song, Jong-Suk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.2
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    • pp.100-106
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    • 2001
  • This investigation was performed to study the influence of benomyl on photosynthetic pigments and enzymes in soybean leaves. Chlorophyll and pheophytin levels were reduced by benomyl 45 days after greening. These results indicate that chlorophyll a and b, and pheophytin must be controlled by benomyl. SDS-PAGE analysis showed that 50 and 14.5 kD polypeptides represented as large and small subunits of rubisco. In the both of these subunits, the band intensity of the control was significantly higher than that after benomyl treatment, indicating that these two subunits are affected by benomyl. Benomyl strongly inhibited both the activity and content of rubisco as its concentration was gradually increased. However, it remains unclear whether this reduction of rubisco level was due to a reduced level of rubisco activase. Two major polypeptides of 46 and 42 kD were identified as rubisco activase subunits by SDS-PAGE. The intensity of these two bands was shown to be higher in the control than after benomyl treatment. These results indicate that the rubisco decrease resulting from increased benomyl concentrations was caused by rubisco activase. A significant decrease in both the activity and content of rubisco activase by benomyl was also observed. There results suggest that the decrease in rubisco level caused by benomyl is accompanied by a decrease in both the activity and content of rubisco activase.

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Effects of Light Enviroment on Photosynthetic Rate and Chlorophyll Contents of Three Broad-leaved Species Growing in the Forest (임분의 광환경이 활엽수 3수종의 광합성율 및 엽록소 함양에 미치는 영향)

  • Kim Gab-Tae
    • Korean Journal of Environment and Ecology
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    • v.19 no.2
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    • pp.188-192
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    • 2005
  • To seek for proper regeneration methods with under-planting, Photosynthetic rate and chlorophyll contents of three broad-leaved species seedlings, Kalopanax pictum, Fraxinus rhynchophylla and Cornus controversa, were measured and compared between growing sites, below the canopy of Larix kaemferi and open land in the forest. Nursery stocks was planted in open nurs- ery in the forest in late April, 1997, and under-planting below the canopy of Larix kaemferi was conducted in late April, 1998. This experiment examined the relationships between shade-tolerance and physiological response to different light environment by tree species. To adapt the shade environment, leaves of Kalopanax pictum might be increased the more chlorophyll con- tents and photosynthetic ability than other species. From these results, shade-tolerance of the tree might be ordered Kalopanax pictum, Fraxinus rhynchophylla, and Cornus controversa.