• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2011년도 추계학술대회 초록집
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• pp.113.1-113.1
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• 2011

미세조류 내의 엽록소는 바이오디젤 전환 반응에서 산 촉매의 활성을 억제 할 뿐만 아니라, 짙은 색상을 띄게하여 바이오디젤 품질규격으로부터 벗어나게 한다. 미세조류의 엽록소 분석은 용매에 의해 엽록소를 추출한 후, 흡광도를 측정하여 그 함량을 계산하는 방법을 널리 사용하고 있다. 건조된 미세조류의 분석은 선택되는 용매에 따라 최대 추출량이 달라지는 것을 제외하고 큰 문제가 없지만 미세조류를 lipid 오일로 변환하면, 용매에 녹지 않아 추출이 되지않는 문제가 발생하여 흡광도 측정을 어렵게 한다. 따라서 미세조류의 형태가 powder일 때와 오일인 경우를 구분하여 용매를 선택해야 하며, 오일 또는 powder 형태 구분 없이 사용할 수 있는 분석법을 적용하여 서로 다른 엽록소 함량을 비교한 후 분석법 간의 상호 장단점을 파악해야한다. 본 연구에서는 메탄올을 용매로 사용하는 분석법(porra et al.)과 아세톤을 용매로 사용하는 분석법(Humphrey and Jeffrey)을 적용하여 엽록소 함량을 비교하였고, AAS(Atomic Absorption Spectrometer)를 통한 Mg 함량 측정을 통해 엽록소 함량을 계산하는 분석법간의 차이를 확인하였다.

• 한국대기환경학회지
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• 제16권2호
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• pp.121-128
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• 2000

To identify the effects of air pollution on several physiological characteristics of Styrax japonica in Yeochon industrial comples soil properties photosynthetic capacity and chorophyll contents were investigated. All of these measurements were taken on Styrax japonica stand at 1. 3 and 7km from an industrial complex which is an air pollution source. In addition a Styrax japonica stand in Sang-ju city was selected as a control Each physiological measurement was in leaves of east-, west- south- and north-facing branches. The results obtained were as follows: 1) Al contents of soil in Yeochon industrial complex were higher than those of Sang-ju city a non-polluted area. higheral contents contributed to decrease in soil pH. 2) Chlorophyll contents of leaves at 1km from the industrial complex closest from the air pollution source were lower than those of 3 and 7km/ 3) Photosynthetic capacity of Styrax japonica at 1km from the air pollution source was lower than those at 3 and 7km.

• ALGAE
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• 제23권4호
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• pp.269-276
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• 2008

We have conducted the study of distribution and inhabitation of the aerial algae from five stone cultural properties located in Gyeonggi-do. A total of 21 taxa were identified, among which 8 taxa of cyanophytes, 12 taxa of cholorophytes and one diatom species. Protococcus viridis, Ulothrix zonata, Synechocystis aquatilis and Chroococcus varius occurred in 15, 9, 8 and 7 study sites, respectively. These coccoid types appeared more frequently than filamentous types. In this study, Chlorococcum infusionum, Cylindrocystis gracilis, Klebsormidium crenulatum, Klebsormidium dissectum and Klebsormidium flaccidium newly recorded from Korea. We found that 80% of stone cultural properties had aerial algae and their 58-84% were grouped into Class 5 based on chlorophyll-a concentration.

• Journal of Ginseng Research
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• 제6권2호
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• pp.149-153
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• 1982

To investigate the effect of light intensity on the growth of shoots and roots, water and chlorophyll contents in the ginseng leaf were determined at 5%, l0%, 20%, 30% light transmittance rate (LTR) with 1,2 and 4 year-old ginseng plants in the field. Stem length, size of the leaf, water and chlorophyll contents were decreased as the increase of the light intensity in all ages of ginseng plants. The degree of decrease was severe in the one year-old ginseng compared to that of 2, or 4 year-old ginseng, while there was no difference between the 2 and 4 year-old plants. Root weight per plant was highest at 5% LTR in the one year-old plants, while it was at 20% LTR in the 2 and 4 year-old plants. Generally, demand of light for the growth of one year-old ginseng was lower than those of 2 or 4 year-old plants there was no difference of it among the ginseng plants older than 2 years.

• Journal of Plant Biology
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• 제27권4호
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• pp.241-251
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• 1984

The present study investigated the effects of triacontanol (TRIA) on plant growth and peroxisomal enzyme activities in radish seedlings. The optimum concentration of TRIA with respect to radish seedling bioassay was decided to 1.0mg $1^{-1}$. In comparison to untreated controls (including Tween 20 treatment), 1.0mg $1^{-1}$ TRIA treatment caused an increase in seed germination rate and root growth, but no stimulation in hypocotyl growth. Chlorophyll accumulation in cotyledon during carly development stage increased rapidly, and degradation of chlorophyll in later stage due to the cotyledon senesence was noticeably retarded. Increase of soluble protein content in cotyledon at early period of development was observed. Isocitrate lyase and catalase activity was not significantly different in both the treated and the untreated plants. But, glycolate oxidase activity was inhibited by TRIA down to 20% against controls. Also, the increase of the activity of peroxidase, a leaf-senescence marker enzyme, was continuously retarded over control for 8 days of development. Based on above results, TRIA was found to be active in both the growth and the peroxisomal enzyme activities of radish seedlings.

• Journal of Plant Biology
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• 제31권2호
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• pp.155-164
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• 1988

The rapid senescence of detached Chinese cabbage leaf discs in darkness is first manifested by a sharp rise in malondialdehyde content (indicated by distruption of membrane structure), then by a rise in peroxidase activity and a decrease in catalase activity, and ultimately by chlorophyll degradation. These changes in parameters besides the catalase activity during senescence were delayed by application of spermidine. Especially, 10-4M spermidine almost completely arrested chlorophyll degradation after incubaton over 5 days. Spermidine reduced the amount of ethylene produced by senescing leaf discs. Additionally, it also reduced IAA-induced ethylene production. Calcium ion (1mM, 10mM) supplied together with the spermidine diminished the spermidine action, indicating probable involvement of an initial ionic attachment mechanism. These results suggest that spermidine can be used as an anti-senescence agent for plants and that this agent may stabilize membrane structure through interaction with the negatively charged loci on the membrane and exert the influence during senescence.

• 한국환경생태학회지
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• 제6권1호
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• pp.9-11
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• 1992

목본식물에 대한 Al독성에 관한 기초자료를 얻고자, Al농도별(1.0, 2.5및 5.0mM)수용액과 지하수에 수경삽목한 개나리삽수의 생장관련형질(신초의 생장, 엽수, 엽피해율 및 엽록소함량)을 처리간 비교하였다. 모든 생장관련형질들(신초의 생장, 엽수, 엽피해율 및 엽록소함량)에서 처리간 고도의 통계적 유의차가 인정되었으며. 1.0mM 정도의 Al수용액도 개나리삽수의 생장을 현저히 억제하였다.

• 한국환경과학회지
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• 제8권1호
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• pp.101-106
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• 1999

The relationship between primary productivity and changes in water quality was investigated at Mulgum station, a site downstream of the Nakdong River, Korea. Phytoplankton production was characterized by blooms of Microcystis aeruginosa during the summer and Stephanodiscus hantzschii during the winter. Primary production and secondary production by bacterioplankton ranged from 1.5~53.5 mg-C/ι day and 0.1~0.3 mg-C/ι day, respectively. Distribution of total organic carbon appeared to be highly correlated with phytoplankton biomass, especially during blooms of M. aeruginosa, when particulate organic carbon was 81% of total organic carbon and the main source of organic materials supplied into the water. The correlation coefficient between chlorophyll-a and BOD was 0.86. Thus it was concluded that autochthonous phytoplankton mostly affected the BOD level. Total bacterial numbers were also highly correlated with chlorophyll-a ($r^2$= 0.84) and the bacterial community appears to be regulated by phytoplankton biomass in this area.

• 한국식품영양과학회지
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• 제28권2호
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• pp.378-382
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• 1999

The sanitizing effect of irradiation on the fresh vegetable extract juices was investigated. Total bacteria, coliform bacteria and total ascorbic acid were determined during the storage periods at 4oC. Chlorophyll, carotenoid, tannin, electron donating ability and peroxidase activity were determined immediately after irradiation. Results showed that the viable cells were detected below the level of 105 CFU/ml during 12 days with doses of 3 and 5 kGy. Total ascorbic acid and tannin contents increased immediately after irradiation. However, irradiation didn't affect chlorophyll and car otenoid contents, electron donating ability, and peroxidase activity. It was considered that irradiation was effective in sanitizing fresh vegetable extract juices.

• Journal of Plant Biology
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• 제36권1호
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• pp.97-104
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• 1993

The lipid composition of thylakoid membranes was compared between mesophyll and bundle sheath chloroplasts of maize. According to mild-denaturing gel electrophoresis, mesophyll thylakoids contained both PS I complex and PS II light-harvesting chlorophyll-protein complex(LHCP), while those of bundle sheath cells contained mainly PS I complex. The amount of lipids per mg chlorophyll was higher in bundle sheath thylakoids than in mesophyll. The major polar lipid classes were monogalactosyldiacylglycerol(MGDG), digalactosyldiacylglycreol, sulfolipid and phosphatidylglycerol (PG) in both tissues. Linolenic acid(18 : 3), linoleic acid(18 : 2) and palmitic acid(16 : 0) were the main fatty acyl components, with higher ratio of unsaturated to saturated fatty acids in bundle sheath thylakoids, suggesting these membranes are more fluid. The most striking difference in lipid composition between the two kinds of tissues was the practical absence of trans- 3-hexadecenoic acid(16 : 1t) in PG of bundle sheath thylakoids. This fatty acid is known to be involved in the association of LHCP as oligomeric form. More than 80% of MGDG molecular species was 18 : 3, 18 : 3, demonstrating that maize is a typical 18 : 3 plant. Therefore, the possibility of the functional relationships between the lamella structure, and thus the distribution of photosystems, and MGDG molecular species was excluded.