• 제목/요약/키워드: $CaCl_2$

검색결과 1,951건 처리시간 0.025초

회분식과 연속식 2단계 생물반응기에서 AcNPV의 곤충세포에의 감염시 배지 첨가물을 이용한 재조합 $\beta$-Galactosidase 생산의 증진 (Improved Recombinant ,$\beta$-Galactosidase Production Using Medium Additives at AcNPV Infection of Insect Cells in Batch and Continuous Two-Stage Bioreactors)

  • 김지선;이기웅
    • KSBB Journal
    • /
    • 제9권3호
    • /
    • pp.294-298
    • /
    • 1994
  • $CaCl_2$, glucose, frutose, glutamine, glutamate 그리고 Ii pids와 같은 배지 첨가물들이 회분식 그리고 연속식 2단계 생물반응기 시스템에셔 재조합 ${\beta}$-galactosidase (${\beta}$-gal) 생산을 증진시키는가를 조사하였다. Sf 21 세포에 AcNPV의 감염시 $CaCl_2$, frutose, glutamate, cholesterol 및 tocoph eral과 같은 배지 첨가물을 첨가하였을 때 ${\beta}$-gal 생산이 증진되었다. 30mM $CaCl_2$, 2.2mM frutose, 4.lmM glutamine, 그리고 O.34mM cholesterol이 보강된 감염 배 지를 이용한 재조합${\beta}$-gal 생산은 약 40% 의 증가를 보였다.

  • PDF

Desalinization Effect of Pennisetum Alopecuroides and Characteristics of Leachate Depending on Calcium Chloride (CaCl2) Concentration

  • Yang, Ji;Yoon, Yong-Han;Ju, Jin-Hee
    • 인간식물환경학회지
    • /
    • 제23권4호
    • /
    • pp.445-453
    • /
    • 2020
  • Background and objective: Calcium chloride (CaCl2) and sodium chloride (NaCl) are commonly used as a deicing agent in South Korea and penetrate the soil on the roadside, causing damage to plants. This study was conducted to investigate the salinity reduction effect of Pennisetum alopecuroides and the chemical characteristics of soil leachate. Methods: The plants were treated with five different concentrations of CaCl2 (0, 1, 2, 5, and 10g·L-1) and were grouped into the Cont., C1, C2, C5, and C10 groups. CaCl2 of 200 m·L-1 was sprayed to each plant once every two weeks. The growth of P. alopecuroides (plant height, leaf length, leaf width and the number of leaves) was measured. The level of EC and pH, and exchangeable cations (K+, Ca2+, Na+, and Mg2+) in the leachate of soil was monitored. Results: The pH of soil leachate decreased as the CaCl2 concentration increased, and the EC increased significantly. The content of K+ did not change significantly until the concentration of CaCl2 reached 5 g·L-1, but the content of Ca2+, Na+, and Mg2+ significantly increased. The plant height, leaf length, and leaf width of P. alopecuroides showed the highest value in CaCl2 1 g·L-1 followed by CaCl2 2 g·L-1 and the control group. Root fresh weight was the highest in CaCl2 2 g·L-1. On the other hand, there was no change in the shoot fresh weight, dry weight and root dry weight, and P. alopecuroides growth inhibition at the concentration of 5 g·L-1 or higher in the plant height and leaf length. Conclusion: P. alopecuroides is relatively highly salt-tolerant and can improve the salt damaged soil by lowering the content of the salt-based exchangeable K+ ions.

Stiffness loss in enzyme-induced carbonate precipitated sand with stress scenarios

  • Song, Jun Young;Sim, Youngjong;Yeom, Sun;Jang, Jaewon;Yun, Tae Sup
    • Geomechanics and Engineering
    • /
    • 제20권2호
    • /
    • pp.165-174
    • /
    • 2020
  • The enzyme-induced carbonate precipitation (EICP) method has been investigated to improve the hydro-mechanical properties of natural soil deposits. This study was conducted to explore the stiffness evolution during various stress scenarios. First, the optimal concentration of urea, CaCl2, and urease for the maximum efficiency of calcite precipitation was identified. The results show that the optimal recipe is 0.5 g/L and 0.9 g/L of urease for 0.5 M CaCl2 and 1 M CaCl2 solutions with a urea-CaCl2 molar ratio of 1.5. The shear stiffness of EICP-treated sands remains constant up to debonding stresses, and further loading induces the reduction of S-wave velocity. It was also found that the debonding stress at which stiffness loss occurs depends on the void ratio, not on cementation solution. Repeated loading-unloading deteriorates the bonding quality, thereby reducing the debonding stress. Scanning electron microscopy and X-ray images reveal that higher concentrations of CaCl2 solution facilitate heterogeneous nucleation to form larger CaCO3 nodules and 11-12 % of CaCO3 forms at the interparticle contact as the main contributor to the evolution of shear stiffness.

[$Ca^{2+}-activated\;Cl^-$ Current in Gastric Antral Myocytes

  • Lee, Moo-Yeol;Bang, Hyo-Weon;Uhm, Dae-Yong;Rhee, Sang-Don
    • The Korean Journal of Physiology
    • /
    • 제28권2호
    • /
    • pp.143-150
    • /
    • 1994
  • The whole-cell mode of the patch clamp technique was used to study $Ca^{2+}-activated\;Cl^-\;current$ $(I_{Cl_{Ca}})$ in gastric antral myocytes. Extracellular application of caffeine evoked $Ca^{2+}-activated\;current$. In order to isolate the chloride current from background current, all known systems were blocked with specific blockers. The current-voltage relationship of caffeine-induced current showed outward rectification and it reversed at around $E_{Cl^-}$. The shift of reversal potential upon the alteration of external and internal chloride concentrations was well fitted with results which were calculated by the Nernst equation. Extracellular addition of N-phenylanthranilic acid and niflumic acid which are known anion channel blockers abolished the caffeine induced current. Intracellular application of a high concentration of EGTA also abolished this current. Application of c-AMP, c-GMP, heparin, or $AIF^-_4$ made no remarkable changes to this current. Sodium replacement with the impermeable cation N-methylglucamine or with $Cd^{2+}$ rarely affected this current. From the above results it is suggested that the caffeine induced current was a $Cl^-$ current and it was activated by intracellular $Ca^{2+}$.

  • PDF

칼슘처리와 MA포장의 저장중 '후지' 사과의 품질에 미치는 영향 (Effects on Quality of 'Fuji' Apple by Dipping of Calcium Solution and MA Packaging)

  • 박형우;김윤호
    • 한국포장학회:학술대회논문집
    • /
    • 한국포장학회 2006년도 정기총회 및 추계학술발표대회
    • /
    • pp.95-101
    • /
    • 2006
  • 거창사과원예협동조합에서 구입한 사과 600상자를 5% $CaCl_2$ 용액에 각각 15분간 침지하는 방법으로 전처리 후 24시간 건조시키고 무포장구, $25{\mu}m$ 두께의 기능성 MA 필름으로 포장한 포장구와 무처리 무포장구인 대조구로 나누어 $0^{\circ}C$의 저온저장고로 옮겨 7개월 동안 저장하면서 3주 간격으로 품질을 분석하였다. 저장 기간이 증가할수록 중량 감소율은 Control 구보다 $CaCl_2$구와 $CaCl_2+MA$구의 중량 감소율이 월등히 낮았다. 경도변화는 저장 기간이 증가할수록 감소하는 경향을 보였으며, 그 중 $CaCl_2$구는 초기치 0.97 $kg_f$에서 저장 18주에는 0.95 $kg_f$로 초기치에 비해 2%의 가장 적은 변화를 보였다. 산도는 대조구보다 처리구의 산도가 높게 나타났으나 큰 차이는 나타나지 않았다. 가용성 고형분 함량은 저장 기간 모든 실험구에 따른 큰 차이가 없었다. 비타민 C 함량은 저장기간이 지날수록 모든 실험구에서 감소하는 경향을 보였으며, 기호도 조사에서 $CaCl_2$구와 $CaCl_2+MA$구가 대조구보다 유의적으로 높게 나타났다.

  • PDF

블렌칭과 $CaCl_2$ 첨가가 건식 절임법으로 제조한 오이지의 저장성 및 품질 특성에 미치는 영향 (Effect of Blanching and $CaCl_2$ on the Quality Characteristics of Oiji Prepared by Dry Salting Method during Storage)

  • 김청희;양윤형;김미리
    • 동아시아식생활학회지
    • /
    • 제15권2호
    • /
    • pp.219-225
    • /
    • 2005
  • Effect of blanching and/or $CaCl_2$ addition on the quality characteristics of Oiji prepared by dry salting method, which has been used for industry, was investigated. Control$(15\%\;salt),\;CAO(15\%\;salt\;+\;0.04\%\;CaCl_2)$ and BCO $(15\%\;salt\;+\;0.04\%\;CaCl_2+blanching\;at\;60^{\circ}C\;for\;20\;min)$ for 165 days. Acidity was lower, but pH was higher in BCO than in the control or CAO. During the whole period of storage, greenness(-a) of BCO was maintained in Hunter color system, compared with the other groups. The numbers of total microbes, lactic acid bacteria and yeast were the lowest in BCO, while the highest in control. Moreover, texture profile analysis exhibited that fracturability and hardness maintained the highest in BCO during storage, compared with the other groups. Based on these results, combination of blanching and $CaCl_2$ addition is favorable to extend the shelf life and to maintain the good quality.

  • PDF

박형 망간전지용 전해질막의 제조 및 전기화학적 특성 (Preparation of Electrolyte Membranes for Thin Manganese Batteries and Its Electrochemical Characteristics)

  • 정순기
    • 한국산학기술학회논문지
    • /
    • 제7권6호
    • /
    • pp.1292-1295
    • /
    • 2006
  • 세 종류의 전해질 수용액(염화암모늄형, 염화아연형, 알칼리형)과 조해성재료($CaBr_2$ 또는 $CaCl_2$)를 filter paper 내부에 함침시켜 망간 일차전지용 전해질막을 제조하였다. 전해질막의 두께는 $250{\sim}300{\mu}m$ 이며 유연성 또한 매우 좋았다. 전해질막의 전기화학적 특성은 수분공급을 위해 첨가된 조해성 재료의 종류 및 함량에 크게 의존하였고, $CaCl_2$가 첨가된 계에서 상대적으로 높은 이온전도도 및 방전용량이 얻어졌다.

  • PDF

염화(鹽化)칼슘의 함량(含量)이 Mortar의 강도(强度)에 미치는 영향(影響)에 관(關)한 연구(硏究) (A Study on the Effect of Calcium-Chloride Content on the Strength of Mortar)

  • 성찬용;강신업
    • 농업과학연구
    • /
    • 제6권2호
    • /
    • pp.185-191
    • /
    • 1979
  • 시멘트의 응결(凝結) 경화촉진제(硬化促進劑)로 사용(使用)되는 염화(鹽化)칼슘 첨가량(添加量)이 mortar의 강도(强度)에 미치는 영향(影響)을 연구(硏究)하기 위하여, 보통 mortar를 경화(硬化)시킨 경우와 $CaCl_2$을 1.0%. 2.0%, 3.0% 첨가(添加)하여 경화(硬化)시킨 경우의 압축강도(壓縮强度)와 인장강도(引張强度)를 배합비별(配合比別), 재영별(材令別)로 구분(區分)하여 비교시험(比較試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 압축강도(壓縮强度)는 염화(鹽化)칼슘을 첨가(添加)한 몰타르가 보통(普通) 몰타르보다 크게 나타났으며, 2.0% 첨가(添加)했을 때 최대강도(最大强度)를 나타냈다. 2.인장강도(引張强度)는 7일(日) 강도(强度)에서 염화(鹽化)칼슘을 첨가(添加)한 몰타르가 보통(普通)몰타르보다 높은 강도(强度)를 나타냈으나, 28일(日) 강도(强度)에서는 1.0% 첨가(添加)한 몰타르가 최대강도(最大强度)를 나타냈고, 2.0% 이상(以上) 첨가(添加)한 경우는 보통(普通)몰타르보다 적게 나타났다. 3. 염화(鹽化)칼슘 첨가량(添加量)이 3.0%이상(以上)일 때는 급결현상(急結現象)이 나타났으며 이로 인(因)한 균열 때문에 강도(强度)는 저하(低下)되는 것으로 사료(思料)된다. 4. 부배합(富配合)에서는 압축(壓縮) 및 인장강도(引張强度)가 재령(材令) 7일(日) 이전(以前)에는 강도증진(强度增進)에 효과(效果)가 컸고 재령(材令) 7일(日) 이후(以後)에는 큰 차이가 없었으므로, 염화(鹽化)칼슘을 1.0%~2.0% 첨가(添加)하여 사용(使用)하면 동기공사(冬期工事)에서 초기강도증진(增初期强度進)을 기(期) 할 수 있을 것으로 생각된다.

  • PDF

Ageing effect on compressibility, permeability and shear strength of clayey soils exposed to salt solutions

  • Cakar, Emel;Yukselen-Aksoy, Yeliz
    • Geomechanics and Engineering
    • /
    • 제25권3호
    • /
    • pp.245-251
    • /
    • 2021
  • The present study investigated the ageing effect on compressibility, permeability and shear strength behavior of kaolin and bentonite samples in the presence of NaCl and CaCl2 solutions. The compressibility, permeability and shear strength parameters were determined on the 60, 190, and 250 days cured samples. The results have shown that, the kaolin sample becomes more compressible in the presence Ca2+ ions with ageing. Generally, the normalized compression index values of bentonite samples increased at the end of 60 days and 250 days curing time periods. The normalized permeability value of kaolin decreased by ageing in the presence of Na+ ions almost twofold. The permeability values of bentonite increased both in NaCl and CaCl2 solutions during ageing. In the presence of Na+ ions kaolin had higher max. shear stress value than Ca2+ ions. When the max. shear stress values of 0, 60 and 190 days samples were compared, it was seen that NaCl solution had no significant effect on the shear strength of kaolin sample. However, the shear strength of kaolin increased in the CaCl2 solution during ageing. In the presence of Ca2+ ions the max. shear stress value of bentonite was higher. The results of this study have shown that ageing has significant effects on the compressibility, permeability and shear strength of kaolinitic and bentonitic clayey soils.

Petunia hybrida와 Nicotiana sanderaer간(間) 원형질체(原形質體) 융합효율증진(融合效率增進) (Improvements of Protoplast Fusion Efficiency between Petunia hybrida and Nicotiana sandarae)

  • 정재동;노영희;지선옥
    • Current Research on Agriculture and Life Sciences
    • /
    • 제10권
    • /
    • pp.147-155
    • /
    • 1992
  • Petunia hybrida와 Nicotiana sanderae의 원형질체(原形質體) 융합(融合)에 의한 체세포잡종식물(體細胞雜種植物)을 얻기 위하여 원형질체융합(原形質體融合)에 미치는 융합제(融合濟)의 종류, PEG의 처리시간(處理時間) 및 융합시(融合時) 온도(溫度), 융합용액(融合溶液)과 희석용액내(稀釋溶液內)의 $CaCl_2{\cdot}2H_2O$의 양(量) 및 희석용액(稀釋溶液)의 pH에 대해 실험(實驗)하였으며 그 결과(結果)는 다음과 같다. 두 종(種)의 원형질체(原形質體)를 $CaCl_2{\cdot}2H_2O$ 5.5mM이 함유(含有)된 PEG 6,000 30%용액(溶液)으로 $25^{\circ}C$에서 10분간 처리(處理)한 후 $CaCl_2{\cdot}2H_2O$ 50mM이 함유(含有)된 희석용액(稀釋溶液)의 pH를 9.0으로 조절(調節)한 용액(溶液)으로 희석(稀釋)시켰을 때 원형질체(原形質體) 융합율(融合率)이 가장 높았다.

  • PDF