• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.73-79
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• 1995

Complementary DNAs (cDNAs) to the coat protein gene of an ordinary Korean strain of potato virus Y (PVY-OK) isolated from potato (cv. Superior) were synthesized and cloned into a plasmid pUC119 and sequenced. The RNA of the virus propagated in tobacco (Nicotinaa sylvestris) was extracted by the method of phenol extraction. The first strand of cDNAs to the coat protein penomic RNA of the virus was made by Moloney murine leukemia virus reverse transcriptase. The cDNA were synthesized and amplified by the method of polymerase chain reaction (PCR) using a pair of oligonucleotide primers. PVYCP3P and PVYCP3M. The size of cDNAs inserted in pUC119 plasmid was estimated as about 840 bp upon agarose gel electrophoresis. Double stranded cDNAs were transformed into the competent cell of E. coli JM109. Sequence analysis of cDNAs was conducted by the dideoxynucleotide chain termination method. Homology of cDNAs of the PVY-OK coat protein genomic RNA with those of PVY-O (Japan), PVY-T (Japan), PVY-TH (Japan), PVYN (The Netherlands),and PVYY (France) was represented as 97.3%, 88.9%, 89.3%, 89.6% and 98.5%, respectively. Homology at the amino acid level turned out to the be 97.4%, 92.5%, 92.9%, 92.9% and 98.5%, respectively.

• Journal of radiological science and technology
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• v.33 no.3
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• pp.179-184
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• 2010

A hard X-ray microscope system for obtaining images of nano-spatial resolution has been widely studied and requires monochromatic X-ray. A multilayer mirror of 84% reflectivity was designed to acquire tungsten characteristic X-ray of 8.4 keV from the white beam generated from an X-ray tube, and the C/W multilayer mirror of $50{\times}50\;mm$ size and 5.65 nm d-spacing was fabricated by the ion-beam sputtering system. The C/W multilayer had a uniformity of 99.5%, and the structure of the multilayer mirror was verified by a TEM image. The obtainable x-ray reflectivity for the C/W multilayer mirror at 8.4 keV was estimated from measuring the X-ray reflectivity using the copper characteristic X-ray of 8.05 keV. Monochromatic X-ray of 8.4 keV was generated by combining a X-ray tube, and the reflectivity and monochromaticity were 77.1% and 0.21 keV, respectively. Monochromatic X-ray generated from the combination of an X-ray tube and an C/W multilayer mirror has enough potential to use X-ray source for hard X-ray microscope system of laboratory size. If the C/W multilayer mirror of d-spacing of a few nanometers can be fabricated, monochromatic X-ray corresponded to 17.5 keV, molybdenum characteristic X-ray, can be obtained and applied to mammography in the medical application.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.215.1-215
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• 2003

Bacillus polymyxa No. 26이 생산하는 생전분 당화형 $\beta$-amylase를 쌀가루 반죽시 첨가하여 45$^{\circ}C$에 5시간 둔 후 증자하여 제조한 절편을 4$^{\circ}C$ 저온실에 40시간 동안 저장하면서 기계적 검사와 관능검사에 의하여 물성과 맛을 측정하고 효소 무 첨가의 경우와 비교하여 노화억제 효과를 조사하였다. 기계적 검사시 효소 무 첨가구의 경우는 40시간까지도 굳지 않아 노화되지 않았으며, 경도, 점착성, 씹힘성에서도 큰 차이를 보였다. 관능검사시에도 경도, 촉촉함성, 단맛에 있어서 효소 첨가효과가 크게 나타났으며 떡의 제조 시 효소의 이용으로 설탕첨가를 생략할 수 있고 소화성과 맛을 좋게하므로서 품질을 향상시킬 수 있었다.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.161-162
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• 2014

The subject of this study, Eco-friendly Polyurethane Waterproofing Membrane Coating of Exposed Type is manufactured by replacing environmental hazardous substance such as Toluene, Dioctyl Phthalate with Dimethyl Carbonate, waste-soybean oil. As part of existing filler is also replaced with waste-rubber chip and waste-soybean oil. As a result of environment friendly tests, in test of detection of VOCs case was contented with the Ministry of Environment standards at 5% below. Testing methods for heavy metal extracted was contented with standards for official wastes test method. So it is judged that environment friendly is secured.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2011.05a
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• pp.77-78
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• 2011

저비용, 고효율, 안정적 수급이 가능한 전력 에너지원이 요구되면서 석탄이 새로운 에너지원으로 급부상하게 됨에 따라 차세대 친환경 석탄화력 발전기술인 IGCC (Integrated Gasification Combined Cycle) 발전 시스템 의 개발이 필요하게 되었다. 석탄가스화 공정(IGCC: integrated coal gasification combined cycle)은 석탄을 가스화한 후 이를 이용하여 복합발전소를 운전하는 발전기술로서 석탄을 고온, 고압아래에서 수소와 일산화탄소를 주성분으로 한 합성가스로 전환한 뒤 합성가스 중에 포함 된 분진과 황 화합물 등 유해물질을 제거하고 천연가스와 유사한 수준으로 정제하여 전기를 생산하는 친환경 발전 기술이다.

• The Korean Journal of Food And Nutrition
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• v.16 no.4
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• pp.359-364
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• 2003

Frozen doughs for danish pastries were made by straight dough method to study the effect of thawing temperature on the quality of pastries. Danish pastry doughs were immediately frozen at -40 $^{\circ}C$ quickly after make-up and stored for 6 weeks at -20$^{\circ}C$ and baked after thawing and fermentation a week. Thawing temperature and fermentation time were controled to 3 hr at 5$^{\circ}C$, 2 hr at 10$^{\circ}C$, and 1.5 hr at 20$^{\circ}C$, respectively. The number of survival yeast cells in dough and the volume, the moisture content, the texture of danish pastries were investigated. Survival yeast cells showed the highest number at thawing temperature of 5 $^{\circ}C$. The volume of danish pastries decreased significantly according to the increase of frozen storage time and danish pastries thawed at 5$^{\circ}C$ revealed the largest volume. In moisture contents of danish pastry, there was no significantly difference without regard to the thawing temperature but showed the highest moisture content at thawing temperature of 20$^{\circ}C$. Danish pastries thawed at 5$^{\circ}C$ accomplished the best texture in the resulting pastry.

• Plant Journal
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• v.10 no.2
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• pp.48-59
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• 2014

Coupons which have same figure as weld joint of the forged steel valves and 1 inch nominal weld thickness were manufactured using ASTM A182 F92 material. After welding with GTAW method, the welded specimens have been post-weld heat treated at $705^{\circ}C$, $735^{\circ}C$, $750^{\circ}C$, $765^{\circ}C$, $795^{\circ}C$ and $825^{\circ}C$ for 1 hour per 1 inch nominal weld thickness each (Group 1) to evaluate characteristics of welds based on various holding temperature. Indeed, 3 welded specimens were post-weld heat treated for 30 minutes, 1 hour and 2 hour (Group 2) at $735^{\circ}C$ to evaluate characteristics of welds based on various holding time. Hardness values were measured at the weld metal, heat affected zone and base metal to observe hardness change depending on the condition. As a result of the evaluation, appropriate holding temperature for PWHT is proved as $750^{\circ}C$ and $765^{\circ}C$ for 1hour per 1 inch nominal weld thickness. Indeed, holding for 1 hour per 1 inch nominal weld thickness was insufficient for PWHT effect when the holding temperature was at $735^{\circ}C$. The microstructure of post-weld heat treated weld metal was determined as tempered-martensite structure.

• Proceedings of KOSOMES biannual meeting
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• 2006.11a
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• pp.1-8
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• 2006

국민소득 증대와 주 5일 근무제 시행으로 육상레저관광에서 해양레저관광으로 그 추이가 이동되고 있다. 해양레저를 즐기고자하는 수요는 증가하고 있으나, 기반시설 부족으로 활성화가 이루어지지 못하고 있는 실정이다. 특히 우리나라 서남해안 해역은 2,000 여개의 다도해가 자연방파제 역할을 하고 있으므로 해양레저 산업을 활성화 시키는데 적합한 천혜의 자연조건을 보유하고 있다. 본 연구에서는 우리나라 해양레저 기반시설의 현황과 문제점을 분석하고, 서남해안 해양레저 산업 활성화에 필요한 친환경 마리나 시설 구축에 관한 자연조건, 해상조건 분석, 다목적용 마리나 시설에 관하여 논한다.

• Journal of Gastric Cancer
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• v.8 no.2
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• pp.79-84
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• 2008

Purpose: With advancements in laparoscopic surgery, there have been efforts to expand the indication for laparoscopic surgery up to advanced gastric cancer. However, scant data are available regarding the feasibility and advantages of laparoscopy-assisted distal gastrectomy (LADG) with standard radical D2 lymph node dissection. Materials and Methods: Twenty-two patients who were preoperatively diagnosed with cT1N0M0 gastric cancer underwent LADG with standard D2 lymphadenectomy between February and August 2007. They were compared with patients who underwent conventional open D2 lymphadenectomy with respect to clinicopathologic features, surgical outcomes, and postoperative course. Results: The mean operative time was significantly longer in the LADG group than in the open group ($160{\pm}25min$ vs. $135{\pm}21min$, P<0.001). However, surgical outcomes, such as surgical margin and number of retrieved lymph nodes ($25.7{\pm}11.1$ vs. $26.9{\pm}9.2$, P=ns) were comparable between the groups. The LADG group exhibited quicker postoperative recovery, and both groups exhibited similar postoperative morbidity and mortality. Conclusion: LADG with D2 lymphadenectomy is feasible and safe, with short-term surgical outcomes comparable to those seen in open D2 lymphadenectomy. Further prospective clinical investigation will be needed to better evaluate the advantages of LADG with D2 lymphadenectomy.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.170.2-170.2
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• 2016

DLC(Diamond like Carbon)는 Diamond와 유사한 물리화학적 특성을 보유한 막으로 고경도 및 우수한 내마모성, 화학적 안정성의 특성을 가지고 있다. DLC는 크게 카본의 막 형성 공정에서 카본 소스에 따라 수소가 포함된 DLC와 무수소DLC로 구분된다. Tetrahedral amorphous carbon (ta-C) 박막은 DLC 박막 중에서 가장 다이아몬드와 유사한 특성을 가지는 박막으로, a:C-H에 비해 높은 열적안정성, 경도(50~60 GPa) 및 내마모 특성이 우수하여, 현재 다양한 응용분야에 적용하고 있다. 본 연구에서는 무수소 DLC 형성을 위해 자장필터가 장착된 Filtered Vacuum Arc Source(FVAS)를 자체적으로 개발하여 연구를 진행하였다. FVAS 장비는 카본 이온 발생부와 Plasma Duct 부위, 전자석부위 구성되어 있으며, 본 연구에서는 Plasma Duct 부위의 Bias 제어를 통해 음극에서 기판으로 이동하는 카본이온의 에너지와 flux 변화를 통한 박막 증착 거동 및 물성 연구를 진행하였다. Plasma Duct Bias 변화는 각 0, 5, 10, 15, 20 V 조건으로 진행하였으며, 물성 평가는 경도(Hardness), 마찰계수, 응력(Stress), 전기전도 특성에 대한 분석을 진행하였다. 박막의 증착 거동에서는 Plasma Duct bias 변화에 따라10 V에서 가장 높은 증착 거동을 가지다 감소하는 경향을 확인 하였으며, 박막의 물성 특성 평가 시에도 이와 유사하게 특성의 차이를 관찰하였다. 이는 음극부위에서 형성된 카본이온이 기판에 도달 시에 Plasma Duct Bias 변화에 따라 이온의 Flux 및 에너지 변화로 인해 박막의 밀도 및 ta-C 막의 물성 변화로 예상되며, 이를 분석하기 위해 라만분석 및 기판 도달 에너지 분석을 진행하였다