• Title/Summary/Keyword: $C_4A_3S$

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Overexpression and Purification of Monooxygenases Cloned from Arthrobacter chlorophenolicus A6 for Enzymatic Decomposition of 4-Chlorophenol (4-Chlorophenol 분해박테리아 Arthrobacter chlorophenolicus A6로부터의 monooxygenase의 복제 및 대량발현과 정제 그리고 기질분해활성도 분석)

  • Ryu, Song-Jung;Kang, Christina S.;Kim, Han S.
    • Journal of Soil and Groundwater Environment
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    • v.19 no.3
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    • pp.47-55
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    • 2014
  • Arthrobacter chlorophenolicus A6 possesses several monooxygenases (CphC-I, CphC-II, and CphB) that can catalyze the transformation of 4-chlorophenol (4-CP) to hydroxylated intermediates in the initial steps of substrate metabolism. The corresponding genes of the monooxygenases were cloned, and the competent cells were transformed with these recombinant plasmids. Although CphC-II and CphB were expressed as insoluble forms, CphC-I was successfully expressed as a soluble form and isolated by purification. The specific activity of the purified CphC-I was analyzed by using 4-CP, 4-chlorocatechol (4-CC), and catechol (CAT) as substrates. The specific activities for 4-CP, 4-CC, and CAT were determined to be 0.312 U/mg, 0.462 U/mg, 0.246 U/mg, respectively. The results of this study indicated that CphC-I is able to catalyze the degradation of 4-CC and CAT in addition to 4-CP, which is a primary substrate. This research is expected to provide the fundamental information for the development of an eco-friendly biochemical degradation of aromatic hydrocarbons.

The Kinematic Analysis of Horizontal Velocity and Records from Start to Finish of the Elite Women 100m Hurdler (엘리트 여자 100m 허들선수의 스타트에서 피니쉬까지 구간별 수평속도 및 기록에 관한 운동학적 분석)

  • Lee, Jung-Ho;Ryu, Jae-Kyun
    • Korean Journal of Applied Biomechanics
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    • v.17 no.3
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    • pp.31-39
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    • 2007
  • The purpose of this study was to observe the kinematic pattern of elite women 100m hurdler race from start to finish and analyze how the change of horizontal velocity makes an influence on the athletes' performance. The analysis was based on the performance of Korean elite 100m hurdler A and international elite hurdlers B and C. Following results were drawn from the analyzation of elite 100m hurdlers' technical characteristics; During the race, hurdler A made more than 8 m/s of horizontal velocity at the 3rd, 4th, 6th, and the 7th hurdle. The horizontal velocity peaked at the 4th hurdle with 8.23 m/s. On the other hand, hurdler B and hurdler C maintained more than 8 m/s of horizontal velocity from the 2nd hurdle through the 10th hurdle. Hurdler B's fastest horizontal velocity was 8.67 m/s from the 6th to the 7th hurdle and hurdler C's fastest horizontal velocity was 8.85 m/s from the 5th to the 8th hurdle. From the start line to the 3rd hurdle, the times achieved by hurdlers A, B, and C were 4.90 sec, 4.65 sec, and 4.52 sec. In the middle of the race, which is from the 4th hurdle to the 7th hurdle, hurdlers A, B, and C ran in 9.10 sec, 8.60 sec, and 8.38 sec. And the latter part of the race to the 10th hurdle, the times hurdlers A, B, and C hit were 12.32 sec, 11.66 sec, and 11.32 sec. To the finish line, it took 1.15 sec for hurdler A, 1.1 sec for B, and 1.06 sec for C. Hence, to set the record of sub-13 sec, hurdler A should improve her acceleration from the start line to the 1st hurdle with the speed more than 5.4 m/s and should maintain more than 8 m/s of horizontal velocity from the 2nd hurdle through the 10th hurdle. In addition, hurdler A should improve her speed endurance to minimize the deceleration of horizontal velocity from the 4th hurdle to the final hurdle. If hurdler A could shorten 0.05 sec of time in each hurdle section, she would be able to set the record under 13 seconds.

Polycrystalline $Y_{3}Fe_{5}O_{12}$ Garnet Films Grown by a Pulsed Laser Ablation Technique (엑시머 레이저 증착기술에 의한 $Y_{3}Fe_{5}O_{12}$ 다결정 박막 제조)

  • Yang, C.J.;Kim, S.W.
    • Journal of the Korean Magnetics Society
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    • v.4 no.3
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    • pp.214-218
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    • 1994
  • $Y_{3}Fe_{5}O_{12}$ based garnet films(thin or thick) offer a great promise for the application of microwave communication components. We investigated the magnetic and crystallographic preperties of $Y_{3}Fe_{5}O_{12}$ thick films prepared by KrF eximer laser ablation of a stoichiometric garnet target. It was possible to obtain almost epitaxially oriented films on $Al_{2}O_{3}$(1102) plane. Although the crystalline quality depends on substrate temperature and $O_{2}$ partial pressure used($Po_{2}$), 4.1m thick films of $4{\pi}M_{s}=1300$ Gauss and $H_{c}=37.5$ Oe were obtained at the substrate temperature of $700^{\circ}C$ with the $Po_{2}$ of 100 mTorr after annealing the as-deposited films at $700^{\circ}C$ for 2 hours. These films are expected to be used for magnetostatic spin wave filters at narrow bandwidth frequency.

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Comparison of Doses of Single Scan PBS and Layered Rescanning PBS Using Moving Phantom in Proton Therapy (양성자 치료에서 Moving Phantom을 이용한 Single Scan PBS와 Layered Rescanning PBS의 선량비교)

  • Kim, Kyeong Tae;Kim, Seon Yeong;Kim, Dae Woong;Kim, Jae Won;Park, Ji Yeon;Jeon, Sang Min
    • The Journal of Korean Society for Radiation Therapy
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    • v.31 no.1
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    • pp.43-49
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    • 2019
  • Purpose : We apply the Layered Rescanning PBS designed to complement the Pencil Beam Scanning(PBS), which is vulnerable to moving organs with the Moving Phantom, and compare the homogeneity with the single scan PBS. Methods and materials: Matrix X (IBA, Belgium) and Moving Phantom (standard imaging, USA) were used. A dose of 200 cGy was measured in the AP direction on a hypothetical tumor $10{\times}10{\times}5cm$. The plan type was planned as 4 kinds of sinlge scan PBS, rescan number 4, 8, 12 times. Were measured three times for each types. During the measurement, the respiratory cycle of the Moving Phantom was generally set to 4 seconds per cycle, and the movement radius in the S-I direction was set to 2 cm. In addition, beam on time was measured. Results : The mean values of $D_{max}$ in the PTV were $246.47{\pm}18.8cGy$, $223.43{\pm}8.92cGy$, and $222.47{\pm}7.7cGy$, $213.9{\pm}6.11cGy$ and the mean values of $D_{min}$ were $165.53{\pm}4.32cGy$, $173.13{\pm}11.94cGy$, $184.13{\pm}8.04cGy$, $182.67{\pm}4.38cGy$ and the mean values of $D_{mean}$ $192.77{\pm}6.98cGy$, $196.7{\pm}4.01cGy$, $198.17{\pm}4.96cGy$, $195.77{\pm}3.15cGy$ respectively. As the number of rescanning increased, the Homogeneity Index converged to 1. The beam on time was measured as 2:15, 3:15, 4:30, 5:37 on average. In the measurement process, in the low dose layer of the MU, the problem was found that it was not rescanned as many times as the set number of rescan. Conclusions : In the treatment of tumors with long-term movements, the application of layered rescanning PBS showed a more uniform dose distribution than single scan PBS. And as the number of rescan increase, the distribution of homogeneity is uniform. Compared with single scan plan and 12 rescan plan, HI value was improved by 0.32. Further studies are expected to be applicable to patients who can not be treated with respiratory synchronous radiation therapy.

Optical Properties of $Bi_2S_3$ Thin films ($Bi_2S_3$ 薄膜의 光學的 特性)

  • Wee, Sung-Dong
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.4
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    • pp.62-66
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    • 1989
  • $Bi_2S_3$ polycrystalline and $Bi_2S_3$ amorphous thin tilm were grown by the evaporation method. The measured lattic parameters were $a=1.708{\AA},\;b=0.351{\AA},\;and\;C=3.943{\AA}$ at substrate temperature $210^{cric}C$ were shown to have the orthorhombic structure. The energy gape of $Bi_2S_3$ polycrystalline that was made from thin film were measured to be 1.375eV at $289^{cric}K.$ The optical band gap of $Bi_2S_3$ amorphous thin film was measured to be 1.71eV at $289^{cric}K.$ It was supposed to mechanism that a photon absorption was changed at the center of 674nm (1.84eV)

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Impact of Firefighters' Protective Clothing and Equipment on Upper Body Range of Motion (소방용 방화복 및 방화 장비에 따른 상반신 관절 각도의 동작 범위 연구)

  • Kim, Seonyoung;Park, Huiju
    • Fashion & Textile Research Journal
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    • v.17 no.4
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    • pp.635-645
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    • 2015
  • This study analyzed the range of motion of upper body in different configurations of firefighters' protective clothing and equipment. The purpose of this study was to understand the influence of firefighters' protective clothing and equipment over upper body motion in order to improve design of firefighters' protective clothing and equipment. 12 firefighters' upper body range of motion was analyzed while performing standing and walking trials in five different garment configurations including turnout ensemble, fire boots and the self-contained breathing apparatus. Analysis of upper body range of motion included spinal joints of L5S1, L4L3, T1C7, and C1Head. During standing trials, garment configurations caused a significant difference in range of motions at joints of L5S1, L4L3, T1C7, and C1Head. Analysis on the mean of range of motions at L5S1 and L4L3, showed that firefighters' waist bent forward significantly to a greater extent while they wore a self-contained breathing apparatus. A significantly increased range of motion was found for T1C7 and C1Head while carrying a self-contained breathing apparatus, which indicated an increase in the extension of the trunk and neck backward to stand upright and look squarely. A significant difference in range of motion was also found for L5S1 and L4L3 during walking trials.

Changes of Lactulose Content during Heat Treatment of Milk (우유의 열처리 및 저장 조건에 따른 Lactulose의 함량 변화)

  • 김철현;백승천;정운현
    • Food Science of Animal Resources
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    • v.22 no.1
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    • pp.50-54
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    • 2002
  • The aim of this work to determine the formation of lactulose during heat treatment process as a contribution to the estabilishment of limits of chemical indicators for different types of heat processed milk and analyze of lactulose for the reconstituted milk added samples. The HTST(75$\^{C}$/15s) and UHT(130$\^{C}$/2∼3s) treatment realized with a pilot plant and heat-treated samples were stroed at 4, 10, 30$\^{C}$ for 4 weeks. Changes in lactulose was evaluated at 7 days intervals. The other heat treatment was sealed in glass tube and heated at 75$\^{C}$ for 10 to 120s and heated at 130$\^{C}$ for 2 to 60s in a thermostatically controlled constant temperature bath of glycerol. The reconstituted milk was made with full fat dry milk that reconstituted with deionized water to 10% total solid, and was added to milk at 10, 20, 30% respectively. The samples processed with a HTST pilot plant showed that lactulose was contained at 1.47∼1.52mg/10()ml and 8.19 ∼8.32mg/100ml for UHT-treated samples. Changes in the lactulose content of heat-treated samples during storage at 4 and 10$\^{C}$ for 4 weeks caused a slight increase, however a noticeable increase was observed at 30$\^{C}$ for 4 week. The glass tube samples showed that high correlations between relative increase in content of lactulose and increasing processing times(75$\^{C}$ : r = 0.986, 130$\^{C}$ : r = 0.987, respectively). Added with reconstituted milk would cause a increase of the lactulose content linear with increasing addition amount(r = 0.982). This results observed for lactulose in commercial milk samples would applied to the detection of chemical changes during heat treatment and illegal use of reconstituted milk.

Exploration of structural, thermal and spectroscopic properties of self-activated sulfate Eu2(SO4)3 with isolated SO4 groups

  • Denisenko, Yu.G.;Aleksandrovsky, A.S.;Atuchin, V.V.;Krylov, A.S.;Molokeev, M.S.;Oreshonkov, A.S.;Shestakov, N.P.;Andreev, O.V.
    • Journal of Industrial and Engineering Chemistry
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    • v.68
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    • pp.109-116
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    • 2018
  • $Eu_2(SO_4)_3$ was synthesized by chemical precipitation method and the crystal structure was determined by Rietveld analysis. The compound crystallizes in monoclinic space group C2/c. In the air environment, $Eu_2(SO_4)_3$ is stable up to $670^{\circ}C$. The sample of $Eu_2(SO_4)_3$ was examined by Raman, Fourier-transform infrared absorption and luminescence spectroscopy methods. The low site symmetry of $SO_4$ tetrahedra results in the appearance of the IR inactive ${\nu}_1$ mode around $1000cm^{-1}$ and ${\nu}_2$ modes below $500cm^{-1}$. The band intensities redistribution in the luminescent spectra of $Eu^{3+}$ ions is analyzed in terms of the peculiarities of its local environment.

Optical Preperties of HgS and HgS : Co Crystals and Films (HgS 및 HgS : Co 결정과 박막의 광학적 특성)

  • 박복남;방태환;김종룡;장우선;최성휴
    • Journal of the Korean Vacuum Society
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    • v.5 no.3
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    • pp.213-217
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    • 1996
  • HgS and HgS: Co crystals and films grown by the slow cooling and the chemical bath deposition method were used to measure their crystal structure and their optical absorption spectra. HgS and HgS: Co crystals are hexagonal structure with the lattice constant $a_0=4.155{\AA}$, $c_0=9.505{\AA}$ for HgS and $a_0=4.148{\AA}$, $c_0=9.462{\AA}$ for HgS and $a_0=4.135{\AA}$, $c_0=9.442{\AA}$ for HgS: Co, respectively. The optical energy gap of these crystals are given as 2.040 eV for HgS and 1.900 eV for HgS: Co, and the optical energy gap of these films were 2.440 eV for HgS and 1.940 eV for HgS: Co at room temperature, respectively.

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Fabrication of $Cu_2/CdS$ solar cell and its characteristics ($Cu_2/CdS$ 태양전지 제작 및 그 특성연구)

  • 유평렬;김현숙;이재윤;강창훈;박은옥;정태수;김택성;양동익;신영진
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.2
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    • pp.315-323
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    • 1997
  • The sing1e crystal of cadmium sulfide was grown by vertical sublimation method. The lattice constants of CdS single crystal by extrapolation method are $a_0=4.139\AA$ and $c_0=6.719\AA$, respectively. The $Cu_2$S/CdS solar cell was fabricated using the single crystal of cadmium sulfide and the CuCl solution. The light- to- dark JV cross over effect of the $Cu_2$S/CdS solar cell was measured after annealing for 2 minutes at $250^{\circ}C$ in air atmosphere. The values of Voc, Jsc, Vop, FF, and efficiency are 0.40 volt, $4.2mA/\textrm{cm}^2$, 0.31 volt, $3.8mA/\textrm{cm}^2$, 0.68 and 3.8 %, respectively. The spectral response of the solar cell shows the peaks at 498 nm (2.49 eV) and 585 nm (2.12 eV).

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