• Journal of Plant Biotechnology
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• 제40권1호
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• pp.49-54
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• 2013

C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.

• The Korean Journal of Ecology
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• 제13권4호
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• pp.331-342
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• 1990

Analysis of vegetation on the pavements and under the trees of streets in Seoul city were investigated from July to November 1989. All of the vascular herbs were 9 orders, 13 families, 30 genera, 37 species and 1 forma. Species diversity and evenness index were higher on the pavements than under the trees of streets. Eragrostis multicaulis with C-4 pathway was a dominant species, and next orders of importace values were Eleusine indica(C-4), Digitaria sanguinalis(C-4), Taraxacum officinale(C-3) and Erigeron annus(C-3). On the pavements and under the trees of streets, light intensity and surface temperature were very high, Also, these sites were affected by many kinds of human interferences. Therefore, it was an example of adaptation to specific ecological environments that C-4 plats were more dominant than C-3, growth forms of C-3 plants were rosette or prostrate, and annual or biennial plants were distributed in these studied sites. Generally, fruits types were grains, achens and capsules. Seeds were very small and some had appendages such as pappus.

• Journal of Plant Biotechnology
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• 제30권4호
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• pp.399-403
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• 2003

환경스트레스에 대한 항산화효소의 보호효과를 이해하고 효과적인 스트레스내성 식물을 개발하기 위하여 CuZnSOD와 APX를 엽록체에 발현시킨 형질전환식물체 (CA식물체)에 강한 빛 (highlight, 1,100$\mu$mol m$^{-2}$ sec$^{-1}$)과 4$^{\circ}C$ chilling을 처리하였다. CA식물체 잎절편에서의 보호효과를 CuZnSOD을 도입한 SOD식물체, APX을 도입된 APX식물체 및 비형질전환식물체 (NT식물체)의 것과 비교하였다. CA식물체의 광계2에서의 광합성 효율 (Fv/Fm)은 highlight와 4$^{\circ}C$ chilling복합처리 1시간째에서 NT식물체에 비해 약 15%의 보호효과를 나타내었고, 광계1에서의 P700 redox state는 처리 후 3시간째에 약 23%의 보호효과를 나타내었다. SOD식물체와 APX식물체의 복합처리에 대한 보호효과는 CA식물체와 NT식물체의 중간 효과를 나타내었다. 이러한 결과는 엽록체에서 CuZnSOD와 APX의 동시발현이 highlight와 chilling에 의한 산화스트레스를 극복하는 데 매우 중요하게 관여함을 직접적으로 제시하는 것이다.

• The Plant Pathology Journal
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• 제36권5호
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• pp.450-458
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• 2020

Pepper and tomato plants infected with two Clavibacter species, C. capsici and C. michiganensis have shown different patterns of disease development depending on their virulence. Here, we investigated how pepper and tomato plants respond to infection by the high-virulent or low-virulent Clavibacter strains. For this, we chose two strains of each Clavibacter species to show different virulence level in the host plants. Although low-virulent strains showed less disease symptoms, they grew almost the same level as the high-virulent strains in both plants. To further examine the response of host plants to Clavibacter infection, we analyzed the expression patterns of plant defense-related genes in the leaves inoculated with different strains of C. capsici and C. michiganensis. Pepper plants infected with high-virulent C. capsici strain highly induced the expression of CaPR1, CaDEF, CaPR4b, CaPR10, and CaLOX1 at 5 days after inoculation (dai), but their expression was much less in low-virulent Clavibacter infection. Expression of CaSAR8.2 was induced at 2 dai, regardless of virulence level. Expression of GluA, Pin2, and PR2 in tomato plants infected with high-virulent C. michiganensis were much higher at 5 dai, compared with mock or low-virulent strain. Expression of PR1a, Osmotin-like, Chitinase, and Chitinase class 2 was increased, regardless of virulence level. Expression of LoxA gene was not affected by Clavibacter inoculation. These results suggested that Clavibacter infection promotes induction of certain defense-related genes in host plants and that differential expression of those genes by low-virulent Clavibacter infection might be affected by their endophytic lifestyle in plants.

• Journal of Plant Biotechnology
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• 제4권3호
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• pp.117-122
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• 2002

Cotton Glutathione S-Transferase(GST: EC 2.5.1.18) was cloned and Gh-5 cDNA was overexpressed in tobacco (Nicotiana tabacum) plants. The transformation of cotton GST in tobacco plant was confirmed by northern blot analysis. Type I and Type II transcript patterns were identified in Gh-5 transgenic tobacco plants. Type I transcripts was only discussed in this paper. Glutathione and 1-chloro-2,4-dinitrobenzene (CDNB) were used as the substrates, and the activity of GST in the type I transgenic plants was about 2.5-fold higher than the non-expressers and wild type tobacco plants. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type I transgenic plants produced functional GST in the cells. Type I showed higher GST specific activity than Type II in the transgenic plants. Control and transgenic seedlings were grown in the growth chamber and under the light at 15$^{\circ}C$, and the effects of cotton GST in the seedlings was evaluated. The growth rate of Gh-5 overexpressors was better than the control and non-transgenic tobacco plants. Salinity tolerance was also analyzed on the seeds of transgenic plants. Seeds of Gh-5 overexpressors and the wild type tobacco seedlings were germinated and grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings at both 50 and 100 mM NaCl solution. But at 0, 150, and 200 mM NaCl concentration, the difference in growth rate was not detected.

• 한국조경학회지
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• 제40권4호
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• pp.127-134
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• 2012

이 연구는 저관리 경량형 옥상녹화시스템을 위한 내한성 식물 선발을 목적으로 6종 식물을 대상으로 저온 처리시간과 온도에 따라 전해질 용출 평가와 재생 검사를 연구하였다. Logistic 비선형 회귀분석을 이용하여 전해질 용출 평가를 통하여 치사 온도는 $-16.1{\sim}-24.4^{\circ}C$ 범위로 예측되었으며, 저온에 대한 저항성은 Sedum reflexum > S. spurium > Ophiopogon japonicus > S. album > S. takevimense > Dianthus chinensis의 순으로 나타났다. 2012년 2월 최저 온도인 $-13.4^{\circ}C$에 모든 식물의 전해질 용출은 50% 이하로 나타났으며, 겨울철 최적기온 $-13.5^{\circ}C$ 이상의 지역에서 식물들을 옥상에서 안정적으로 생육될 수 있을 것으로 예측되었다.

• 한국응용곤충학회지
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• 제25권4호
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• pp.201-207
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• 1986

침입해충(侵入害蟲)인 온실가루이의 분포현황(分布現況), 온도(溫度)가 발육(發育)에 미치는 영향(影響) 및 주내분포(株內分布)에 관(關)한 조사결과(調査結果)는 다음과 같다. 1. 온실가루이는 영구온실내(永久溫室內)의 철쭉, 란타나, 포인세치아 등(等) 27과(科) 39종(種)의 관상식물(觀賞植物)에서 기생(寄生)하였다. 2. 산란전기간(産卵前期間)은 $25^{\circ}C$에서 1.1일(日), 산란기간(産卵期間)은 28.8일(日), 산란후기간(産卵後期間)은 0.5일(日) 성충수명(成蟲壽命)은 26.8일(日)로 $25^{\circ}C$가 적온(適溫)이었고, 숫컷의 성충수명(成蟲壽命)이 약(約) 6일(日) 짧았다. 3. 일당(日當) 산란수(産卵數)는 $25^{\circ}C$에서 9.8개(個), 총산란수(總産卵數)는 305개(個) 였다. 4. 난기간(卵期間)은 $25^{\circ}C$에서 8.2일(日), 유충기간(幼蟲期間)은 8.3일(日), 용기간은 7.5일(日)이었고, 한세대(世代)를 거치는데는 $22^{\circ}C,\;25^{\circ}C,\;30^{\circ}C$에서 각각(各各) 27.5일(日), 23.8일(日), 18.9일(日)로 온도(溫度)가 증가(增加)할수록 짧아지는 경향(傾向)이였다. 5. 난(卵)에서 유충(幼蟲)까지의 발육영점온도(發育零點溫度)는 $8.8^{\circ}C$였다. 6. 난(卵)에서 성충(成蟲)까지의 성공율(成功率)은 $22^{\circ}C,\;25^{\circ}C,\;30^{\circ}C$에 각각(各各) 70.3%, 58.0%, 66.4%였다. 7. 온실가루이 한세대기간(世代期間)동안의 성충(成蟲)과 난(卵)은 상위엽(上位葉)에 유충(幼蟲)은 중위엽(中位葉)에 용은 하위엽(下位葉)에 집중분포(集中分布)하였다.

• The Plant Pathology Journal
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• 제31권4호
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• pp.363-370
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• 2015

Using the Chinese cabbage (Brassica campestris) cultivar 'Chun-goang' as a host and turnip mosaic virus (TuMV) as a pathogen, we studied the effects of ambient temperature ($13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$ and $33^{\circ}C$) on disease intensity and the speed of systemic infection. The optimal temperature for symptom expression of TuMV was $18-28^{\circ}C$. However, symptoms of viral infection were initiated at $23-28^{\circ}C$ and 6 days post infection (dpi). Plants maintained at $33^{\circ}C$ were systemically infected as early as 6 dpi and remained symptomless until 12 or 22 dpi, depending on growth stage at the time of inoculation. It took 45 days for infection of plants grown at $13^{\circ}C$. Quantitative realtime polymerase chain reaction (q-PCR) results showed that the accumulation of virus coat protein was greater in plants grown at $23-28^{\circ}C$. The speed of systemic infection increased linearly with rising ambient temperature, up to $23^{\circ}C$. The zero-infection temperature was $10.1^{\circ}C$. To study the effects of abruptly elevated temperatures on systemic infection, plants inoculated with TuMV were maintained at $10^{\circ}C$ for 20 d; transferred to a growth chamber at temperatures of $13^{\circ}C$, $18^{\circ}C$, $23^{\circ}C$, $28^{\circ}C$, or $33^{\circ}C$ for 1, 2, or 3 d; and then moved back to $10^{\circ}C$. The numbers of plants infected increased as duration of exposure to higher temperatures and dpi increased.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 2005년도 제36차 춘계학술대회 및 제3회 HVEM 이용자 워크샵
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• pp.36-40
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• 2005

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.189-189
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• 2022

Soil salinity negatively affects plant growth, productivity, and metabolism. Rice is known to have more sensitive phenotypes than other cereal crops, such as wheat, sorghum, and barley. We characterized the molecular function of rice C3HC4 as a really interesting new gene (RING). Oryza sativa RING finger protein H2-3 (OsRFPH2-3) was highly expressed in 100 mM NaCl. To identify the localization of OsRFPH2-3, we fused vectors that include C-terminal GFP protein (35S;;OsRFPH2-3-GFP). OsRFPH2-3 was expressed in the nucleus in rice protoplasts. An in vitro ubiquitin assay demonstrated that OsRFPH2-3 possessed E3-ubiquitin ligase activity. However, the mutated OsRFPH2-3 were not possessed any E3-ubiquitin ligase activity. Under normal conditions, there is no significant phenotypic difference between transgenic plants and WT plants. However, OsRFPH2-3-overexpressing plants exhibited higher fresh weight and length under saline conditions. Also, transgenic plants maintain higher chlorophyll, proline, and soluble sugar contents and lower H₂O₂ and MDA contents than the wild type; these results support transgenic plants with enhanced salinity tolerance phenotypes.