• Title/Summary/Keyword: $C_3$-plant

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A Study on Fractionation and Characterization of Water-Soluble Natural Fe-Chelates From Garbage Compost and Activated Sewage Sludge (활성오니(活性汚泥) 및 진개퇴비중(塵芥堆肥中) 수용성(水溶性) 철(鐵) 킬레이트의 분리(分離)와 특성(特性)에 관(關)한 연구(硏究))

  • Park, Nae-Joung;Lindsay, W.L.
    • Applied Biological Chemistry
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    • v.18 no.4
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    • pp.194-202
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    • 1975
  • This study was conducted to study the properties of the water-soluble natural chelating agents from garbage compost and activated sewage sludge responsible for Fe chelation, which is closely associated with the effectiveness in correcting iron chlorosis in plant. The water-soluble fraction of these materials was fractionated by menas of Sephadex gel filtration and the fractions of Fe chehates were traced by radioactive $^{59}Fe$. The fractions were examined by ultraviolet and infrared. spectroscopy and stability constants for Fe. The water-soluble fraction from garbage compost was separated by Sephadex G-25 into approximately four fractions. Most of the added $^{59}Fe$ was associated with fraction I, which appeared at the void volume. Further fractionation by Sephadex G-50 indicated that the molecular weight of water-soluble chelating agents is in the approximate range of 5000 to 10,000. The water-soluble fraction from activated sewage sludge gave six fractions by Sephadex G-25. Most of the added $^{59}Fe$ was found in the fraction I,II, and III, The molecular weights of most chelating agents associated with $^{59}Fe$ appeared to be less than 5,000 and those of fraction I that appeared at the void volume was in the range of 5,000 to 1,000. Discrepancy between radio activity count and UV absorption indicated the heterogeneity of the fractions obtained by Sephadex gel filtration. Ultraviolet absorption spectra of all fractions separated by Sephadex G-25 and containing chelating agents showed no differences. Fraction IV and V of sewage extract showed absorption maxima and shifting similar to nucleic acid components suggesting the presence of decomposition products of nucleic acid. Similarity fraction VI contained phenolic type amino acid groups. Fraction I of compost extract contained most of the added $^{59}Fe$ and showed weak but extra definite absorption in the 1230, and $1270cm^{-1}$ region, suggesting that extra oxygen groups in polyphenolic structure were probably involved in Fe chelation. In sewage extract, fraction I,II, and III in which most of the $^{59}Fe$ was found, showed strong definite polypeptide absorption in the region of $1540cm^{-1}$ due to NH deformation and C-N stretching of amide groups in the peptidebond. These extra functional groups in fraction I, II, and III appeared to be associated with Fe chelation. The other fractions, not associated with $^{59}Fe$, still have carboxyl and hydroxyl groups, suggesting that these functional groups in these water extracts may not independently form the Fe chelates. Precipitation of ferric hydroxide precluded measuring the stability constants for Fe-chelates. However, the formation constants for Zn chelates as log K values for compost extract and sewage extract at pH 4.0 from which the strength of chelation with Fe could be presumed, were 8.23, and 9.75, respectively, indicating strong complexation with metals. The chelating capacity of compost extract containing 6.5 g organic matter per liter was 0.82 mM, and that of sewage extract containing 5.3 g per liter was 0. 64 mM.

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Genesis and Classification of the Red-Yellow Soils derived from Residuum on Acidic and Intermediate Rocks -II. Songjeong series (산성암(酸性岩) 및 중성암(中性岩)의 잔적층(殘積層)에 발달(發達)한 적황색토(赤黃色土)의 생성(生成) 및 분류(分類) -제(第)II보(報) 송정통(松汀統)에 관(關)하여)

  • Um, Ki Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.2
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    • pp.75-81
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    • 1973
  • The morphological, physical, and chemical properties of Sonjeong series derived from acidic crystalline rocks are presented. Also it deals with the genesis and classification of the Songjeong series. Morphologically these soils have brown to dark brown loam A horizons and yellowish red to red clay loam Bt horizons with moderate, medium subangular blocky structure and thin patchy clay cutans on the ped faces. C horizons are very deep, yellowish red to yellowish brown fine sandy loam or sandy loam with original rock structure. Physically distribution of particle size indicates that clay increases with depth up to argillic horizons but below the argillic horizons clay content decrease. The moisture holding capacity is fairly good in Songjeong soils. Chemically soil reaction is strongly to very strongly acid throughout the profile and content of organic matter is less than 1 per cent except A horizons. Cation exchange capacity ranges from 5 to 9 me/100g of soils and base saturation is less than 35 per cent throughout the profile. The natural fertility of Songjeong soils are usually low. It needs lime, organic matter, and heavy application of fertilizer for the crop land. These soils occur temperate and humid climate under coniferous, deciduous, and mixed forest vegetation. Songjeong soils are classified as Red-Yellow Soils. Characteristically Songjeong soils are similar to Red-Yellow Podzolic soils in the United States but lack of A2 horizons and are quite liket Red-Yellow Soils of the Japan. According to new classification system which is 7th approximation of USDA Songjeong soils can be classified as fine loamy, mesic family of Typic Hapludults and in the FAO/UNESCO project World Soil Map as Orthic Acrisols.

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Studies on the Desertification Combating and Sand Industry Development(III) - Revegetation and Soil Conservation Technology in Desertification-affected Sandy Land - (사막화방지(沙漠化防止) 및 방사기술개발(防沙技術開發)에 관한 연구(硏究)(III) - 중국(中國)의 황막사지(荒漠沙地) 녹화기술분석(綠化技術分析) -)

  • Woo, Bo-Myeong;Lee, Kyung-Joon;Choi, Hyung-Tae;Lee, Sang-Ho;Park, Joo-Won;Wang, Lixian;Zhang, Kebin;Sun, Baoping
    • Journal of Korean Society of Forest Science
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    • v.90 no.1
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    • pp.90-104
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    • 2001
  • This study is aimed to analyze and to evaluate the revegetation and soil conservation technology in desertification-affected sandy land, resulting from the project of "Studies on the desertification combating and sand industry development". Main native plants for combating desertification : The general characteristics of vegetation distribution in desertified regions are partially concentrated vegetation distribution types including the a) desert plants in low zone of desert or sanddune of depressed basin, b) salt-resistant plants around saline lakes, c) grouped vegetation with Poplar and Chinese Tamarix of freshwater-lakes, saline-lakes and river-banks, d) gobi vegetation of gravel desert and e) grassland and oasis-woods around the alluvial fan of rivers, etc. Generally, Tamarix ehinensis Lour., Haloxylon ammodendron Bunge., Calligonum spp., Populus euphratica Oliver., Elaeagnus angustifolia L., Ulmus pumila L., Salix spp., Hedysarum spp., Caragana spp., Xanthoceras sorbifolia Bunge., Nitraria tangutorum Bobr., Lespedeza bicolor, Alhagi sparsifolia Shap., Capparis spinosa L., Artemisia arenaria DC., etc. are widely distributed in desertified regions. It is necessary for conducting research in the native plants in desertified regions. Analysis of intensive revegetation technology system for combating desertification : In the wind erosion region, the experimental research projects of rational farming systems (regional planning, shelterbelts system, protection system of oasis, establishment of irrigation-channel networks and management technology of enormous farmlands, etc.), rational utilization technology of plant resources (fuelwood, medicinal plants, grazing and grassland management, etc.), utilization technology of water resources (management and planning of watershed, construction of channel and technology of water saving and irrigation, etc.), establishment of sheltetbelts, control of population increase and increased production technology of agricultural forest, fuelwood and feed, etc. are preponderantly being promoted. And in water erosion region, the experimental research projects of development of rational utilization technology of land and vegetation, engineering technology and protection technology of crops, etc. are being promoted in priority. And also, the experimental researches on the methods of utilization of water (irrigation, drainage, washing and rice cultivation, etc.), agricultural methods (reclamation of land, agronomy, fertilization, seeding, crop rotation, mixed-cultivation and soil dressing works, etc.) and biological methods (cultivation of salt-resistant crops and green manure and tree plantation, etc.) for improvement of saline soil and alkaline soil in desertified-lands are actively being promoted. And the international cooperations on the revegetation technology development projects of desertified-lands are sincerely being required.

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Development of Late Bolting and New Deep Red Leaf with Wrinkled Lettuce 'Chunpungjeokchukmyeon' (추대가 늦고 색깔이 진한 새로운 잎상추 '춘풍적축면' 육성)

  • Jang, Suk-Woo;Hur, Youn-Young;Choi, Mi-Ja;Kwon, Young-Seok;Kim, Jeom-Sun;Lee, Jong-Nam;Lee, Eung-Ho;Seo, Myeong-Hun;Park, Jae-Ho;Jang, Ik;Jang, Mi-Hyang;Hwang, Hae-June;Ko, Sun-Bo
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.627-631
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    • 2010
  • A new cultivar of lettuce (Lactuca sativa L.) with wrinkled traverse elliptic and deep red leaf, 'Chunpungjeokchukmyeon' which has late bolting and deep red expression leaf color was developed from a cross between 'Pojabijeokchukmyeon' (red leaf color and late bolting) and 'Meokchima' (Deep red and low yield). The cross and selection for advanced lines had been done by the pedigree method during 2000-2007. The advanced lines were evaluated for yield and adaptability at several locations in Korea (Gangwon-do, Gyeonggi-do, Chungcheongbuk-do, Jeollabuk-do, Gyeongnam-do, and Jeju-do) from 2008 to 2009. The 'Chunpungjeokchukmyeon' has gray seed color and traverse elliptic leaves. The type of matured stage is medium shape between 'chukmyeon' and 'chima' leaf lettuce. Compared to 'Dukseomjeokchukmyeon', marketable yield of 'Chunpungjeokchukmyeon' was higher by 6% (at 372 g per plant) and 'Chunpungjeokchukmyeon' has particularly improved expression of deep red leaf color in high temperature cultivation in the field. The shelf-life of 'Chunpungjeokchukmyeon' was three weeks longer than 'Dukseomjeokchukmyeon' at 4$^{\circ}C$. The anthocyanin content of 'Chunpungjeokchukmyeon' was higher than that of 'Dukseomjeokchukmyeon' with 17.5 mg/100g. The BSL (latucin+8-deoxylactucin+lactucopicrin) content of 'Chunpungjeokchukmyeon' is lower than that of 'Dukseomjeokchukmyeon'. Furthermore, its taste is better, more crispy, and sweeter than those of 'Dukseomjeokchukmyeon'. So we recommend that new cultivar 'Chunpungjeokchukmyeon' can be suitable for cultivation in spring season than summer season.

Physiochemical properties and anti-proliferative activity against human lung cancer cells of kombucha using sugar extracts of Cudrania tricuspidata fruits (꾸지뽕나무 열매 당침액 이용 콤부차의 이화학적 품질 특성 및 인체 폐암세포 항증식 활성)

  • MiAe Cho;Kyoung Ju Song;Jinwook Lee;Chang Moo Lee;Ho Chil Choi;Jong Cheol Park
    • Food Science and Preservation
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    • v.31 no.4
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    • pp.673-681
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    • 2024
  • This study was conducted to develop kombucha with better functionality. The developed kombucha (CK) was prepared using the sugar extracts from fruits of Cudrania tricuspidata (Carrière) Bureau ex Lavallée instead of the sugar, which is used as a substrate for SCOBY in conventional kombucha (K). During fermentation, the soluble solids content significantly decreased in CK compared to K, and the pH change decreased rapidly in CK compared to K. On the 14th day of fermentation, the weight of the SCOBY in CK was higher than that in K. Immediately after preparation, K contained only sucrose, but CK contained sucrose, glucose, and fructose. SCOBY appears to use glucose and fructose preferentially during fermentation. K contained acetic acid and citric acid right after preparation. However, as fermentation progressed, the composition changed to acetic acid, citric acid, and lactic acid. At the same time, CK initially consisted of citric acid, lactic acid, and acetic acid. However, acetic acid and citric acid increased but lactic acid decreased significantly on the 14th day of fermentation. In the cytotoxicity studies, the CK showed a proliferation-promoting effect on normal lung cells (MRC-5) and strong cytotoxicity against human lung cancer cells (A549). These results suggest that the kombucha made from sugar extracts of C. tricuspidata fruits can be used as a more functional beverage than regular kombucha.

Relationships between Micronutrient Contents in Soils and Crops of Plastic Film House (시설재배 토양과 작물 잎 중의 미량원소 함량 관계)

  • Chung, Jong-Bae;Kim, Bok-Jin;Ryu, Kwan-Sig;Lee, Seung-Ho;Shin, Hyun-Jin;Hwang, Tae-Kyung;Choi, Hee-Youl;Lee, Yong-Woo;Lee, Yoon-Jeong;Kim, Jong-Jib
    • Korean Journal of Environmental Agriculture
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    • v.25 no.3
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    • pp.217-227
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    • 2006
  • Micronutrient status in soils and crops of plastic film house and their relationship were investigated. Total 203 plastic film houses were selected (red pepper, 66; cucumber, 63; tomato, 74) in Yeongnam region and soil and leaf samples were collected. Hot-water extractable B and 0.1 N HCl extractable Cu, Zn, Fe, and Mn in soil samples and total micronutrients in leaf samples were analyzed. Contents Zn, Fe, and Mn in most of the investigated soils were higher than the upper limits of optimum level for general crop cultivation. Contents of Cu in most soils of cucumber and tomato cultivation were higher than the upper limit of optimum level, but Cu contents in about 30% of red pepper cultivation soils were below the sufficient level. Contents of B in most soils of cucumber and tomato were above the sufficient level but in 48% of red pepper cultivation soils B were found to be deficient. Micronutrient contents in leaf of investigated crops were much variable. Contents of B, Fe, and Mn were mostly within the sufficient levels, while in 71% of red pepper samples Cu was under deficient level and in 44% of cucumber samples Cu contents were higher than the upper limit of sufficient level. Contents of Zn in red pepper and cucumber samples were mostly within the sufficient level but in 62% of tomato samples Zn contents were under deficient condition. However, any visible deficiency or toxicity symptoms of micronutrients were not found in the crops. No consistent relationships were found between micronutrient contents in soil and leaf, and this indicates that growth and absorption activity of root and interactions among the nutrients in soil might be important factors in overall micronutrient uptake of crops. For best management of micronutrients in plastic film house, much attention should be focused on the management of soil and plant characteristics which control the micronutrient uptake of crops.

The Effects of Nitrogen Type and Fertilized Depth on Leaching and Absorption of Nitrogen in Paddy Soil and Growth and Yields of Rice (질소비료(窒素肥料)의 형태(形態)와 시용심도(施用深度)가 질소(窒素)의 용탈흡수(溶脫吸收) 및 수도(水滔) 생육수량(生育收量)에 미치는 영향(影響))

  • Maeng, D.W.;Cho, C.Y.;Lee, D.S.
    • Korean Journal of Soil Science and Fertilizer
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    • v.1 no.1
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    • pp.43-60
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    • 1968
  • In order to find the effects of nitrogen type (ammonium sulfate and urea fertilizer) and fertilized depth, (0~10cm, 0cm, 5~10cm, 10~15cm, 15~20cm, and 20cm below) on leaching and absorption of nitrogen in paddy soil, and growth and yields of rice, the pot culture experiment was carried out, using the variety Jaekun, one of the Korean leading variety. Experimental results were Summarized as follows: 1. No variations of the pH of percolating water were induced by the differences of nitrogen types and their fertilized depth (Table. 2). 2. The leaching of nitrogen was less in ammonium sulfate and top soil fertilizing plots than in urea and subsoil fertilizing plot, and the growth of rice in early stage was more promoted in ammonium sulfate and topsoil fertilizing plots (Table. 1, 7 and 8). 3. Leachng of nitrogen through the percolating water almost came to an end at the most numerous tiller stage (Table 1). 4. The absorption of nitrogen of each part of the rice plant in the harvesting stage correlated closely with the yields of each part (Table 5, 6, 9 and 10) and the leaching of nitrogen in the early stage was inversely proportion to the absorption of nitrogen of rice plant in the harvesting time (Table 1, 5, 6, 9 and 10). 5. The number of spikes was more numerous in ammonium sulfate plots than in urea plots on an average, so that the yields were higher in the ammonium sulfate plots than in urea plots although no differences in the grain number per spike were found in above two plots. The number of spikes was more numerous in topsoil fertilizing plots than in subsoil fertilizing plots, but the grain number per spike was less in former than in latter, so that no difference in yields was found. The absorption of nitrogen correlated closely with the yields in complete paddy grains (Table 5, 9, and 10). 6. At the ammonium sulfate fertilizing plots, the number of spikes was more numerous in topsoil fertilizing plots than in subsoil fertilizing plots, (among the each of the topsoil plots, 0~10cm and 5~10cm fertilizing plots kept more spikes than the 0cm fertilizing plots), but the grain number per spike was less in former than in latter (among the each of topsoil plots, no differences were found), so that no significant difference in yields was showed between the topsoil and subsoil fertilizing plots, but the results showed the tendency that the yields were highest in 0~10cm plots and the lowest in 20cm below plots. At the urea fertilizing plots, the number of spikes decreased in proportion to the increasing of fertilized depth, but no variations were found in the grain number per spike, so that the yields decreased in proportion to the increasing of fertilized depth. The absorption of nitrogen correlated closely with the yields in complete paddy grains (Table 5, 6, 9, and 10). 7. When fertilized in topsoil, the number of spikes was more numerous in ammonium sulfate plot than in urea plot, but the grain number per spike variated reversely, so that no differences were found in the yields between the ammonium sulfate and the urea plots, when fertilized in subsoil, both the number of spikes and the grain number per spike were larger in ammonium sulfate than in urea plot, so that the yields were also higher in ammonium sulfate plots (Table 5, 6, 9 and 10). 8. The weight of straw and its nitrogen absorption were higher in ammonium sulfate plot than in urea plot and decreased in proportion to the increasing of fertilized depth. Among the each of topsoil fertilizing plots, the 0~10cm and the 5~10cm fertilizing plots excelled the 0cm plot (Table 5, 6, 9 and 10). 9. No significant variations in the fertilizer treatments were found in the characters of heading date, maturing date, length of culm, length of spike, weight of empty grain, 1,000 grain weight, and one liter weight.

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Analytical Studies on Yield and Yield Components in Barley (대맥의 수량 및 수량구성요소에 관한 해석적 연구)

  • Chung-Yun Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.18
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    • pp.88-123
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    • 1975
  • To obtain useful fundamental informations for improving cultural practices of barley, an investigation was made on the influences of different fertilizer level and seeding rate as well as seeding date on yield and yield components and their balancing procedure using barley variety Suwon # 18, and at the same time, 8 varieties including Suwon # 18 were also tested to clarify the varietal responses in terms of their yield and yield components under different seeding date at Crop Experiment Station, Suwon, during the period of 1969 and 1970. The results obtained were summarized as follows; 1. Days to emergence of barley variety Suwon # 18 at Suwon, took 8 to 19 days in accordance with given different seeding date (from Sept. 21 to Oct. 31). Earlier emergence was observed by early seeding and most of the seeds were emerged at 15$0^{\circ}C$ cumulated soil temperature at 5cm depth from surface under the favorable condition. 2. Degree of cold injury in different seeding date was seemed to be affected by the growth rate of seedlings and climatic condition during the wintering period. Over growth and number of leaves less than 5 to 6 on the main stem before wintering were brought in severe cold damage during the wintering period. 3. Even though the number of leaves on the main stem were variable from 11 to 16 depending upon the seeding date. this differences were occurred before wintering and less variation was observed after wintering. Particularly, differences of the number of main stem leaves from September 21 to October 11 seeding date were occurred due to the differences of number of main stem leaves before wintering. 4. Dry matter accumulation before wintering was high in early seeded plot and gradually decreased in accordance with delayed seeding date and less different in dry matter weight was observed after wintering. However, the increment rate of this dry matter was high from regrowth to heading time and became low during the ripening period. 5. Number of tillers per $\m^2$ was higher in early seeding than late one and dense planting was higher in the number of tillers than sparse planting. Number of tillers per plant was lower in number and variation in dense planting, and reverse tendency was observed in sparse planting. By increasing seedling rate in early seeding date the number of tiller per plant was remarkably decreased, but the seeding rate didn't affect the individual tillering capacity in the late seeding date. 6. Seedlings were from early planting reached maximum tillering stage earlier than those from the late planting and no remarkable changes was observed due to increased seeding rate. However. increased seeding rate tends to make it earlier the maximum tillering stage early. 7. Stage of maximum tillering was coincided with stage of 4-5 main stem leaves regardless the seeding date. 8. Number of heads per $\m^2$ was increased with increased seeding rate but considerable year variation in number of heads was observed by increased fertilizer level. Therefore, it was clear that there is no difficulties in increasing number of heads per $\m^2$ through increasing both fertilizer level and seeding rate. This type of tendency was more remarkable at optimum seeding time. In the other hand, seeding at optimum time is more important than increasing seeding rate, but increasing seeding rate was more effective in late seeding for obtaining desirable number of heads per $\m^2$. 9. Number of heads per $\m^2$ was decreased generally in all varieties tested in late seeding, but the degree of decrease by late seeding was lower in Suwon # 18. Yuegi, Hangmi and Buheung compared with Suwon # 4, Suwon # 6, Chilbo and Yungwolyukak. 10. Highly significant positive correlations were obtained between number of head and tillers per $\m^2$ from heading date in September 21 seeding, from before-wintering in October 1 seeding and in all growth period from October 11 to October 31 seeding. However, relatively low correlation coefficient was estimated between number of heads and tillers counted around late March to early April in any seeding date. 11. Valid tiller ratio varied from 33% to 76% and highest yield was obtained when valid tiller ratio was about 50%. Therefore, variation of valid tiller ratio was greater due to seeding date differences than due to seeding rate. Early seeding decreased the valid tiller ratio and gradually increased by delaying seeding date but decreased by increasing seeding rate. Among the varieties tested Suwon # 18, Hangmi, Yuegi as well as Buheung should be high valid tiller ratio not only in late seeding but also in early seeding. In contrast to this phenomena, Chilbo, Suwon # 4, Suwon # 6 and Yungwolyukak expressed low valid tiller ratio in general, and also exhibited the same tendency in late seeding date. 12. Number of grains per spike was increased by increasing fertilizer level and decreased by increasing seeding rate. Among the seeding date tested. October 21 (1969) and October 11 (1970) showed lowest number of grains per spike which was increased in both early seeding and late seeding date. There were no definite tendencies observed along with seeding date differences in respective varieties tested. 13. Variation of 1000 grain weight due to fertilizer level applied, seeding date and seeding rate was not so high as number of grains per spike and number of heads per $\m^2$, but exhibited high year variation. Increased seeding rate decreased the 1000 grain weight. Among the varieties tested Chilbo and Buheung expressed heavy grain weight, while Suwon # 18, Hangmi and Yuegi showed comparatively light grain weight. 14. Optimum seeding date in Suwon area was around October 1 to October 11. Yield was generally increased by increasing fertilizer level. Yield decrease due to early seeding was compensated in certain extent by increased fertilizer application. 15. Yield variations due to seeding rate differences were almost negligible compare to the variations due to fertilizer level and seeding date. In either early seeding or law fertilizer level yield variation due to seeding rate was not so remarkable. Increment of fertilizer application was more effective for yield increase especially at increased seeding rate. And also increased seeding rate fairly compensated the decrease of yield in late seeding date. 16. Optimum seeding rate was considered to be around 18-26 liters per 10a at N-P-K=10.5-6-6 kg/10a fertilizer level considering yield stabilization. 17. Varietal differences in optimum seeding date was quite remarkable Suwon # 6, Suwon # 4. Buheung noted high yield at early seeding and Suwon # 18, Yuegi and Hangmi yielded higher in seeding date of October 10. However, Buheung showed late seeding adaptability. 18. Highly significant positive correlations were observed between yield and yield components in all treatments. However, this correlation coefficient was increased positively by increased fertilizer level and decreased by increased seeding rate. Significant negative correlation coefficients were estimated between yield and number of grains per spike, since increased number of heads per m2 at the same level of fertilizer tends to decrease the number of grains per spike. Comparatively low correlation coefficients were estimated between 1000 grain weight and yield. 19. No significant relations in terms of correlation coefficients was observed between number of heads per $\m^2$ and 1000 grain weight or number of grains per head.

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Tissue Culture Method as a Possible Tool to Study Herbicidal Behaviour and Herbicide Tolerance Screening (조직배양(組織培養) 방법(方法)을 이용(利用)한 제초제(除草劑) 작용성(作用性) 및 제초제(除草劑) 저항성(抵抗性) 검정방법(檢定方法) 연구(硏究))

  • Kim, S.C.;Lee, S.K.;Chung, G.S.
    • Korean Journal of Weed Science
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    • v.6 no.2
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    • pp.174-190
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    • 1986
  • A series of laboratory and greenhouse experiments were conducted to find out the possibility of tissue culture and cell culture methods as a tool to study herbicidal behaviour and herbicide tolerance screening from 1985 to 1986 at the Yeongnam Crop Experiment Station. For dehulled-rice culture, pure agar medium was the most appropriate in rice growth campared to other media used for plant tissue culture method. All the media but the pure agar medium resulted in growth retardance by approximately 50% and this effect was more pronounced to root growth than shoot growth. Herbicidal phytotoxicity was enhanced under light condition for butachlor, 2.4-D, and propanil while this effect was reversed for DPX F-5384 and CGA 142464, respectively. And also, herbicides of butachlor, chlornitrofen, oxadiazon, and BAS-514 resulted in more phytotoxic effect when shoot and root of rice were exposed to herbicide than root exposure only while other used herbicides exhibited no significant difference between two exposure regimes. Similar response was obtained from Echinochloa crusgalli even though the degree of growth retardance was much greater. Particularly, butachlor, 2.4-D, chlornitrofen, oxadiaxon, pyrazolate and BAS-514 totally inhibited chlorophyll biosynthesis even at the single contact of root. Apparent cultivar differences to herbicide were observed at the young seedling culture method and dehulled rice cultivars were more tolerant in DPX F-5384, NC-311, pyrazolate and pyrazoxyfen, respectively. For derant than other types or rice cultivar in butachlor, pretilachlor, perfluidone and oxadiazon while Tongil-type rice cultivars were more tolerant in DPXF-5384, NC-311, Pyrazolate and Pyrazoxyfen, respectively. For dehulled rice culture, on the other hand, Japonica-type rice cultivar was less tolerant to herbicides of butachlor, propanil, chlornitrofen and oxadiazon that was reversed trend to young seedling culture test. Cultivar differences were also exhibited within same cultivar type. In general, relatively higher tolerant cultivars were Milyang 42, Cheongcheongbyeo, Samgangbyeo, Chilseoungbyeo for Tongil-type, Somjinbyeo for Japonica-type and IR50 for Indica-type, respectively. The response of callus growth showed similar to dehulled rice culture method in all herbicides regardless of property variables. However, concentration response was much sensitive in callus response. The concentration ranges of $10^{-9}M-10^(-8)M$ were appropriate to distinguish the difference between herbicides for E. crusgalli callus growth. Among used herbicides, BAS-514 was the most effective to E. crusgalli callus growth. Based on the above results, tissue culture method could be successfully used as a tool for studying herbicidal behaviour and tolerance screening to herbicide.

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Characteristics and classification of paddy soils on the Gimje-Mangyeong plains (김제만경평야(金堤萬頃平野)의 답토양특성(沓土壤特性)과 그 분류(分類)에 관(關)한 연구(硏究))

  • Shin, Yong Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.5 no.2
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    • pp.1-38
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    • 1972
  • This study, designed to establish a classification system of paddy soils and suitability groups on productivity and management of paddy land based on soil characteristics, has been made for the paddy soils on the Gimje-Mangyeong plains. The morphological, physical and chemical properties of the 15 paddy soil series found on these plains are briefly as follows: Ten soil series (Baeggu, Bongnam, Buyong, Gimje, Gongdeog, Honam, Jeonbug, Jisan, Mangyeong and Suam) have a B horizon (cambic B), two soil series (Geugrag and Hwadong) have a Bt horizon (argillic B), and three soil series (Gwanghwal, Hwagye and Sindab) have no B or Bt horizons. Uniquely, both the Bongnam and Gongdeog series contain a muck layer in the lower part of subsoil. Four soil series (Baeggu, Gongdeog, Gwanghwal and Sindab) generally are bluish gray and dark gray, and eight soil series (Bongnam, Buyong, Gimje, Honam, Jeonbug, Jisan, Mangyeong and Suam) are either gray or grayish brown. Three soil series (Geugrag, Hwadong and Hwagye), however, are partially gleyed in the surface and subsurface, but have a yellowish brown to brown subsoil or substrata. Seven soil series (Bongnam, Buyong, Geugrag, Gimje, Gongdeog, Honam and Hwadong) are of fine clayey texture, three soil series (Baeggu, Jeonbug and Jisan) belong to fine loamy and fine silty, three soil series (Gwanghwal, Mangyeong and Suam) to coarse loamy and coarse silty, and two soil series (Hwagye and Sindab) to sandy and sandy skeletal texture classes. The carbon content of the surface soil ranges from 0.29 to 2.18 percent, mostly 1.0 to 2.0 percent. The total nitrogen content of the surface soil ranges from 0.03 to 0.25 percent, showing a tendency to decrease irregularly with depth. The C/N ratio in the surface soil ranges from 4.6 to 15.5, dominantly from 8 to 10. The C/N ratio in the subsoil and substrata, however, has a wide range from 3.0 to 20.25. The soil reaction ranges from 4.5 to 8.0. All soil series except the Gwanghwal and Mangyeong series belong to the acid reaction class. The cation exchange cpacity in the surface soil ranges from 5 to 13 milliequivalents per 100 grams of soil, and in all the subsoil and substrata except those of a sandy texture, from 10 to 20 milliequivalents per 100 grams of soil. The base saturation of the soil series except Baeggu and Gongdeog is more than 60 percent. The active iron content of the surface soil ranges from 0.45 to 1.81 ppm, easily-reduceable manganese from 15 to 148 ppm, and available silica from 36 to 366 ppm. The iron and manganese are generally accumulated in a similar position (10 to 70cm. depth), and silica occurs in the same horizon with that of iron and manganese, or in the deeper horizons in the soil profile. The properties of each soil series extending from the sea shore towards the continental plains change with distance and they are related with distance (x) as follows: y(surface soil, clay content) = $$-0.2491x^2+6.0388x-1.1251$$ y(subsoil or subsurface soil, clay content) = $$-0.31646x^2+7.84818x-2.50008$$ y(surface soil, organic carbon content) = $$-0.0089x^2+0.2192x+0.1366$$ y(subsoil or subsurface soil, pH) = $$-0.0178x^2-0.04534x+8.3531$$ Soil profile development, soil color, depositional and organic layers, soil texture and soil reaction etc. are thought to be the major items that should be considered in a paddy soil classification. It was found that most of the soils belonging to the moderately well, somewhat poorly and poorly drained fine and medium textured soils and moderately deep fine textured soils over coarse materials, produce higher paddy yields in excess of 3,750 kg/ha. and most of the soils belonging to the coarse textured soils, well drained fine textured soils, moderately deep medium textured soils over coarse materials and saline soils, produce yields less than 3,750kg/ha. Soil texture of the profile, available soil depth, salinity and gleying of the surface and subsurface soils etc. seem to be the major factors determining rice yields, and these factors are considered when establishing suitability groups for paddy land. The great group, group, subgroup, family and series are proposed for the classification categories of paddy soils. The soil series is the basic category of the classification. The argillic horizon (Bt horizon) and cambic horizon (B horizon) are proposed as two diagnostic horizons of great group level for the determination of the morphological properties of soils in the classification. The specific soil characteristics considered in the group and subgroup levels are soil color of the profile (bluish gray, gray or yellowish brown), salinity (salic), depositonal (fluvic) and muck layers (mucky), and gleying of surface and subsurface soils (gleyic). The family levels are classified on the basis of soil reaction, soil texture and gravel content of the profile. The definitions are given on each classification category, diagnostic horizons and specific soil characteristics respectively. The soils on these plains are classified in eight subgroups and examined under the existing classification system. Further, the suitability group, can be divided into two major categories, suitability class and subclass. The soils within a suitability class are similar in potential productivity and limitation on use and management. Class 1 through 4 are distinguished from each other by combination of soil characteristics. Subclasses are divided from classes that have the same kind of dominant limitations such as slope(e), wettness(w), sandy(s), gravels(g), salinity(t) and non-gleying of the surface and subsurface soils(n). The above suitability classes and subclasses are examined, and the definitions are given. Seven subclasses are found on these plains for paddy soils. The classification and suitability group of 15 paddy soil series on the Gimje-Mangyeong plains may now be tabulated as follows.

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