• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.3
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• pp.208-220
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• 2019

The seasonality of leaf fall has important implications for understanding the response of trees' phenology to climate change. In this study, we quantified the leaf fall pattern with a model to estimate the timing and speed of leaf litter according to species and considered the nutrient use strategy of canopy species. In the autumns of 2015 and 2016, leaf litter was collected periodically using 36 litter-traps from the deciduous forests in Gwangneung and sorted by species. The seasonal leaf fall pattern was estimated using the non-linear regression model of Dixon. Additionally, the resorption rate was calculated by analyzing the nitrogen concentration of the leaf litter at each collection time. The leaf litter generally began in early October and ended in mid-November depending on the species. At the peak time (T50) of leaf fall, on average, Carpinus laxiflora was first, and Quercus serrata was last. The rate of leaf fall was fastest (18.6 days) for Sorbus alnifolia in 2016 and slowest (40.8 days) for C. cordata in 2015. The nitrogen resorption rates at T50 were 0.45% for Q. serrata and 0.48% for C. laxiflora, and the resorption rate in 2015 with less precipitation was higher than in 2016. Since falling of leaf litter is affected by environmental factors such as temperature, precipitation, photoperiod, and $CO_2$ during the period attached foliage, the leaf fall pattern and nitrogen resorption differed year by year depending on the species. If we quantify the fall phenomena of deciduous trees and analyze them according to various conditions, we can predict whether the changes in leaf fall timing and speed due to climate change will prolong or shorten the growth period of trees. In addition, it may be possible to consider how this affects their nutrient use strategy.

• Journal of Animal Science and Technology
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• v.50 no.5
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• pp.677-686
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• 2008

This study was conducted to evaluate the effects of dietary wild-ginseng adventitious root meal on growth performance, blood characteristics and meat quality characteristics in growing-finishing pigs. Ninety six pigs[(Landrace×Yorkshire)×Duroc] with average initial body weight of 68.29±0.31kg were used in 70d growth trial. Dietary treatments included 1) CON(Basal diet), 2) WGR1(Basal diet+0.5% wild- ginseng adventitious root meal), 3) WGR2(Basal diet+1.0% wild-ginseng adventitious root meal) and 4) WGR3(Basal diet+1.5% wild-ginseng adventitious root meal). The pigs were allotted into four dietary treatments with six replicate pens and four pigs per pen in a completely randomized design. For the whole period, final body weight and ADG were increased in CON treatment compared to WGR3 treatment(Linear effect, P=0.005). In blood characteristics, red blood cell(RBC) was significantly increased in CON and WGR2 treatments compared to WGR1 treatment (Quadratic effect, P=0.019). WGR2 treatment resulted in higher white blood cell(WBC) than CON and WGR1 treatments(Linear effect, P=0.041). WBC difference was significantly improved in WGR2 treatment compared to other treatments (Linear effect, P=0.042). Total protein was increased in WGR2 treatment compared to CON treatment (Quadratic effect, P=0.011). In cholesterol concentration of blood, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglyceride were not significantly different among treatments. In meet quality, pH in WGR1 treatment was higher than WGR3 treatment(Quadratic effect=0.022). Water holding capacity(WHC) was significantly increased in WGR2 treatment compared to WGR3 treatment(Quadratic effect, P=0.050).

• Applied Chemistry for Engineering
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• v.9 no.3
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• pp.457-461
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• 1998

The vesicle system of DPPC(dipalmitoylphosphaticylcholine)/Chol(Cholesterol) has been modified by incorporating various mole fractions of flourinated surfactant($C_8F_{17}(CH_2)_2OCO-CH_2CH(SO_3Na)COO(CH_2)_2C_8F_{17}$. Sodium bis(1H,1H,2H,2H-heptadecaflurododecyl)-2-sulfosuccinate, FS)/fluorinated fatty acid salt ($C_7F_{15}COONH_4$, ammoniumpentadecaflurooctyrate, FFS), and their physicochemical properties have been investigated in an attempt to enhance the stability of phospholipid vesicle system. The ${\zeta}$-potential measurement by use of Zetamaster sub-micron Particle Electrophoresis Analyzer (Malvern Co.) showed that a charged homogeneous DPPC/Chol/FS vesicle has been formed owing to the incorporated FFS effect on the membrane, playing a role as a cosurfactant in the bilayer between DPPC and FS components. With increase in the concentration of FFS, it was found that the particle size and also surface charge of the DPPC/Chol/FS vesicle decreased. The stability of DPPC/Chol/FS/FFS liposome was found to be enhanced significantly compared to that of DPPC/Chol/FS according to the dispersity change as a function of time. The release rate of dye molecule of Methylene Blue from the DPPC/Chol/FS/FFS vesicle was determined to be slower than that of DPPC/Chol/FS system, and it may be attributed to the increase in microviscosity of the hydrophobic region in the bilayer. The affinfinity of DPPC/Chol/FS/FFS vesicles to albumin was found to be slightly lowered compared to that of DPPC/Chol/FS. Based on these findings, it was confirmed that a more stable and homogeneous vesicle system of DPPC/Chol/FS could be prepared by addition of FFS, acting as a cosurfactant in the aggregate formation.

• Journal of Mushroom
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• v.11 no.4
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• pp.254-260
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• 2013

The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanol extracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) and extracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometric methods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidant activities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanol extract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and 5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and 1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinase inhibitory activity of the methanol extract (200 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than those of positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus and stipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6 mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higher proliferation rate than those of 0.04% adenosine.

• Polymer(Korea)
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• v.29 no.6
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• pp.543-548
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• 2005

Double-layered spheres play an important role in controlling drug delivery for pharmaceutical application, because of the low initial burst compared with single-layered spheres and targetable delivery to specific organ. But it has drawback in loading drug and controlling size. In this study, we developed double-layered spheres using relatively simple oil-in-water (O/W) solvent evaporation method witw/without ultrasonication and investigated the size variation of the double-layered microspheres on the contents of poly(lactide- co-glycolide) (PLGA). Double - layered spheres were char-acterized by scanning elecron microscope (SEM), camscope, and confocal fluorescence laser microscope (CFLM). Double-layered spheres showed smooth surfaces and obvious difference between core and corona by SEM observation and camscope. We observed the fluorescent core in the double-walled spheres composed of FlTC-dextran and PLGA using CFLM. It was found that the core of the microsphere was dextran and the corona of the fabricate microsphere was PLGA. Also, the more PLGA concentration, the more the size of the fabricating double-layered sphere observed.

• Journal of Oriental Neuropsychiatry
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• v.25 no.1
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• pp.1-12
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• 2014

Objectives: In this study, the meditation programs (${\alpha}$ version), which are properly coordinated according to the motion, breathing, and relaxation, are evaluated and researched upon to have positive effects on stress and in the area of psychology. Methods: Approved by the Clinical Trials Deliberation Committee in Oriental Medicine, Dongguk University, Ilsan Hospital, this study collected data according to the applicant's consents, demographic information and anthropometry for the Sasang Constitutional diagnosis. Sasang Constitutional diagnosis measured the beta tools by Institute of Oriental Medicine and a decision tree was made for the Sasang Constitutional questionnaires. The STAI, STAXI, BDI, and HRV were measured before and after the meditation in order to compare the effects of meditation according to Sasang Constitution. The HRV was used as a ProComP KM Tech (co). Results: 1) The positive changes available in the Time-domain analysis of heart rate variability assessment showed that the peace of mind is increased. By analyzing the Sasang constitution, So-eum In's peace of mind included a physical stability of the autonomic nervous system. 2) According to the psychological scale evaluation, each depression scale, trait anger, anger-in, state anxiety and trait anxiety index proved significantly positive effects. By analyzing the Sasang constitution, Eun-In which involved So-eum In and Tae-eum In, had positive effects. 3) The psychological scale changed the group of diagnosed depression or anxiety, it did not mean that the psychological scale changes in the depression group, but the index of the anxiety group had been significantly reduced. This program had clinical effects for anxious patients and Eum-In which involved Tae-eum In and So-eum In according to the analysis of Sasang constitution. 4) Correlations between the gender of each psychological scale showed that women have overall low correlations, but, there were no significant changes. Conclusions: The meditation program developed by adequately mixing Action, relaxation and breathing shows that it is effective for overall Eum-in physical and mental relaxation and concentration. In the future, It will have to be developed Meditation program to show the same effect for all people.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.08a
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• pp.386-386
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• 2012

For white light emitting diode (LED) applications, it has been reported that Y3Al5O12:Ce3+ (YAG:Ce) in nano-sized phosphor performs better than it does in micro-sized particles. This is because nano-sized YAG:Ce can reduce internal light scattering when coated onto a blue LED surface. Recently, there have been many reports on the synthesis of nano-sized YAG particles using bottom-up method, such as co-precipitation method, sol-gel process, hydrothermal method, solvothermal method, and glycothermal method. However, there has been no report using top-down method. Top-down method has advantages than bottom-up method, such as large scale production and easy control of doping concentration and particle size. Therefore, in this study, nano-sized YAG:Ce phosphors were synthesized by a high energy beads milling process with varying beads size, milling time and milling steps. The beads milling process was performed by Laboratory Mill MINICER with ZrO2 beads. The phase identity and morphology of nano-sized YAG:Ce were characterized by X-ray powder diffraction (XRD) and field-emission scanning electron microscopy (FESEM), respectively. By controlling beads size, milling time and milling steps, we synthesized a size-tunable and uniform nano-sized YAG:Ce phosphors which average diameters were 100, 85 and 40 nm, respectively. After milling, there was no impurity and all of the peaks were in good agreement with YAG (JCPDS No. 33-0040). Luminescence and quantum efficiency (QE) of nano-sized YAG:Ce phosphors were measured by fluorescence spectrometer and QE measuring instrument, respectively. The synthesized YAG:Ce absorbed light efficiently in the visible region of 400-500 nm, and showed single broadband emission peaked at 550 nm with 50% of QE. As a result, by considering above results, high energy beads milling process could be a facile and reproducible synthesis method for nano-sized YAG:Ce phosphors.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.2
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• pp.199-206
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• 2008

Simultaneous nitrification and denitrification means that nitrification and denitrification occur concurrently in the same reaction vessel under low DO concentration. Some mathematical models developed to simulate simultaneous nitrification and denitrification reaction, but they have the complex model structures or have limitations of model application. To solve these problems, if possible that predict the behavior of simultaneous nitrification and denitrification reaction by activated sludge model, structures of the model is less complex than previous models and applies the various operation conditions. But original activated sludge models have difficulties in representing the denitrification reaction under aerobic condition. So the aim of this study is to interpret simultaneous nitrification and denitrification reaction by modifying activated sludge model. Original activated sludge model No.1(ASM1) was selected and modified. The simulation result in modified ASM1 predicted appropriately for the measured data. This indicates the structures of ASM1 are properly improved for interpretation of simultaneous nitrification and denitrification reaction.

• Journal of Periodontal and Implant Science
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• v.28 no.4
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• pp.769-786
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• 1998

The purpose of the present study is to examine the effect of cell proliferation and alkaline phosphatase activity in osteoblastic cells and to compare the bone healing ability of rat calvarial defects between the control group and the safflower seed extract treated group. Osteoblastic cells were obtained from calvariae of a fetal rat. Cells were cultured containing DMEM and safflower seed extract ($10^{-6}g/ml$, $10^{-3}g/ml$) at $37^{\circ}$ with 5% $CO_2$ in 100% humidity for 3 days. MTT was performed to examine the viability of the cells, and alkaline phosphatase activity was analyzed to examine the mineralization in vitro. Rat calvarial defects($5{\times}5mm$) in 250g Sprague-Dawly were made using round bur. Rats were administrated with safflower seed extract(0.35g/kg/day) for experimental periods. Calvarial defects were studied histopathologically and immunohistochemically at 1,4, and 8 weeks. High concentration group($10^{-3}g/ml$) of safflower seed extract significantly increased in the cell proliferation and alkaline phos phatase synthesis in osteoblastic cells. The infiltration of inflammatory cells and osteoclastic activities were decreased in the safflower seed extract treated group as compared with control group. Bone maturation was accelerated in the safflower seed extract treated group as compared to control group. No difference in osteoinductive process was observed between the control and the safflower seed extract treated group. Immunohistochemical observation revealed that protein expression of TGF-$\beta$and osteonectin during early healing phase in the safflower seed extract treated group was slightly increased as compared to control group. These results indicate that safflower seed extract promotes the healing process in bony defect of rat calvariae, and retains a potential applicability as an adjuvant therapeutic modality for regeneration of periodontal bony defect.

• Korean Journal of Environmental Agriculture
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• v.18 no.2
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• pp.174-178
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• 1999

An anthocyanin-deficient tomato (Lycopersicon esculentum Mill.) strain, H957, shows an unusual tolerance to low phosphorus (P). To investigate whether the tolerance originates from a tissue/cellular strength, plant tissue culture procedure was employed which facilitate to characterize the tolerance independent of morphological features. The tolerance was analyzed by comparing H957 against H883, its maternal wild type, while each explant was co-cultured on minimal P media. Comparisons were made in fresh weight, dry weight, callus and shoot formation, mineral contents, and P utilization ratios at $0-400{\cdot}\bar{I}MP$, . Growth of the two strains was severely impaired at 0 and $12.5{\cdot}\bar{I}MP.\;At\;25-200{\cdot}\bar{I}MP$, however, H957 consistently showed a greater fresh and dry weight than H883. Shoot onset of H957 was less delayed than H883 compared to optimal P conditions. H957 tissue contains an overall lower P concentration than H883. These observations indicate that H957 may tolerate to low P by its tissue or cellular strength in P utilization side from its morphology.