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Comparison of Antiplatelet Activities of Green Tea Catechins

  • Cho, Mi-Ra;Jin, Yong-Ri;Lee, Jung-Jin;Lim, Yong;Kim, Tack-Joong;Oh, Ki-Wan;Yoo, Hwan-Soo;Yun, Yeo-Pyo
    • Journal of Food Hygiene and Safety
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    • v.22 no.3
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    • pp.223-230
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    • 2007
  • We have previously reported that green tea catechins(GTC) displayed potent antithrombotic effect, which was due to the antiplatelet activity. In the present study, the antiplatelet activity of each green tea catechin components was compared in vitro. Galloylated catechins including (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), (-)-epicatechin gallate (ECG) and (-)-catechin gallate (CG), significantly inhibited collagen $(5{\mu}g/mL)-induced$ rabbit platelet aggregation with $IC_{50}$ values of 79.8, 63.0, 168.2 and $67.3{\mu}M$, respectively. EGCC GCG and CG also significantly inhibited arachidonic acid (AA, $100{\mu}M$)-induced rabbit platelet aggregation with $IC_{50}$ values of 98.9, 200.0 and $174.3{\mu}M$, respectively. However catechins without gallate moiety showed little inhibitory effects against rabbit platelet aggregation induced by collagen or AA compared with galloylated catechins. These observations suggest that the presence of gallate moiety at C-3 position may be essential to the antiplatelet activity of catechins and the presence of B ring galloyl structure may also contribute to the antiplatelet activity of GTC. In line with the inhibition of collagen-induced platelet aggregation, EGCG caused concentration-dependent decreases of cytosolic calcium mobilization, AA liberation and serotonin secretion. In contrast, epigallocatechin (EGC), a structural analogue of EGCG lacking a galloyl group in the 3' position, although slightly inhibited collagen-stimulated cytosolic calcium mobilization, failed to affect other signal transductions as EGCG in activated platelets. Taken together, these observations suggest that the antiplatelet activity of EGCG may be due to inhibition of arachidonic acid liberation and inhibition of $Ca^{2+}$ mobilization and that the antiplatelet of EGCG is enhanced by the presence of a gallate moiety esterified at carbon 3 on the C ring.

The Effect of Rubus coreanum Miquel Against Lipopolysaccharide-induced Oxidative Stress and Lipid Metabolism (복분자 추출물이 LPS로 유도된 산화적 스트레스와 지질대사에 미치는 영향)

  • Kim, In-Deok;Kang, Kum-Suk;Kwon, Ryun-Hee;Yang, Jeong-Ok;Lee, Joong-Sook;Ha, Bae-Jin
    • Journal of Food Hygiene and Safety
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    • v.22 no.3
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    • pp.213-217
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    • 2007
  • LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.

Soil Physical and Chemical Properties with Plantation Regions and Stand Age in Pinus rigida and Larix kaempferi Plantations (리기다소나무와 낙엽송 인공림의 지역 및 임령에 따른 토양 특성)

  • Yang, A-Ram;Hwang, Jaehong;Cho, Minseok;Song, Sun-Wha
    • Journal of Korean Society of Forest Science
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    • v.102 no.4
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    • pp.578-586
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    • 2013
  • This study was performed in Pinus rigida and Larix kaempferi plantations which occupy approximately 60% of artificial forest area in Korea. The objective of this study was to know the differences in soil physical and chemical properties between both plantations. Soil physical and chemical properties from published literature and analyzed soil data in national forest in 2010 and 2011 were analyzed by plantation regions and stand age of 5 years unit. Jeollanamdo in Pinus rigida plantations and Gyeongsangbuk-do in Larix kaempferi plantations showed higher soil chemical properties than those of other regions. Soil texture in both plantations was almost loam and sandy loam. Mean soil pH in Pinus rigida and Larix kaempferi plantations were 4.86 and 4.87, respectively and there was no relationship between soil pH and stand age. The mean concentrations of total nitrogen (%) and available phosphorus (mg $kg^{-1}$) were 0.21 and 11.00 for Pinus rigida plantation and 0.28 and 13.32 for Larix kaempferi plantation. In Larix kaempferi plantation, total nitrogen, available phosphorus and organic matter concentrations and C.E.C. were higher than those in Pinus rigida plantation and showed positive relationship with stand age. This positive relationship was also revealed between the exchangeable cations and soil pH. The results of this study provide an informative data in selecting tree species for planting and contribute to the establishing forest management plan for the maintenance of sustainable forests resources.

The Selection of Appropriate Sampler for the Assessment of Macrobenthos Community in Saemangeum, the West Coast of Korea (새만금 외해역에서 대형 저서동물 군집 조사를 위한 적정 채집기의 선택)

  • 유재원;김창수;박미라;이형곤;이재학;홍재상
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.3
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    • pp.285-294
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    • 2003
  • To select an appropriate sampler for the environmental monitoring survey in coastal waters of Saemangeum, Jeollabuk-do, a macrobenthic sampling was conducted in April 2002. Employed samplers were dredge (type Charcot), a semi-quantitative sampler and Smith-McIntyre (SM) and van Veen grab (VV) as quantitative ones. One haul was tried for dredge and 3 replicates (0.1 ㎡${\times}$3) for SM and W at each of 11 stations. Comparisons of sediment volume in sampler bucket and of precision of biological parameters (i.e., density, biomass, species number and diversity index, H') were made between SM and VV. Sediment volume was significantly different (SM > VV) at p-value of 0.0050 (paired t-test) and, in average, 3 replicate samples of SM and VV satisfied a precision level of 0.2 by applying 4th root transformation. Patterns of observed and expected species numbers and H' were compared. Dredge-VV samples showed higher affinity than any other pair. Several dominant species in the area were underestimated in dredge samples (e.g., polychaete Heteromastus filiformis. Aricidea assimilis etc.). Quantifying the agreement pattern of multi-species responses was accomplished by estimating correlations between similarity matrices. Correlation between dredge and VV was slightly higher, but near-per-fect matches were found in general. Different ranks and composition among principal species lists were presumably linked to the effect of penetration depth that differs among samplers. Lower level of some species' abundance in VV samples (ca. 50% compared with those of SM) was explained in this context. It seem appropriate to regard the effect as a probable cause of relatively higher correlations in dredge-VV, Overall bio-logica1 features indicated that a better choice could be SM in situations of requiring high data quality. The others work well, however, on observing and defining faunal characteristics and their capability cannot be questionted if we do not expect a first-order quality.

Vegetation structure and environmental factors in Paksil-nup Wetland, Hapcheon (박실늪의 식생 구조와 환경 요인)

  • Kim, Cheol-Soo;Lee, Pal-Hong;Son, Sung-Gon;Oh, Kyung-Hwan
    • Journal of Wetlands Research
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    • v.2 no.1
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    • pp.31-40
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    • 2000
  • The flora, vegetation structure and physico-chemical characteristics of surface sediment were investigated in Paksil-nup wetland of Hapcheon-gun, Gyeongsangnam-do, Korea in 1990~1998. Ranges of the surface sediment characteristics such as pH, conductivity, organic matter, total nitrogen, available phosphorus, exchangeable K, exchangeable Ca, exchangeable Mg, and exchangeable Na were 4.36~4.34, $19.0{\sim}1260.0\;{\mu}mho\;cm^{-1}$, 0.01~6.35%, 0.001~0.14%, 0.01~0.31 mg/l00g, 1.01~13.98 ppm, 16.75~143.80 ppm, 0.93~14.85 ppm and 0.21~3.86 ppm, respectively. Percentages of the particle size such as sand, silt, and clay were 13.0~93.3%, 5.4~71.7%, and 0.5~37.5%, respectively. The flora of the study area was composed of 72 families, 182 genera, 223 species, 36 varieties and 2 form or total 261 kinds, and those were 45 kinds of vascular hydrophytes and 216 kinds of vascular hygrophytes. The life form of vascular hydrophytes was classified as 27 kinds (60%) of emergent plants, 8 kinds (18%) of submerged plants, 6 kinds (13%) of free-floating plants and 4 kinds (9%) of floating-leaved plants, respectively. The importance value of Salix nipponica was highest as 123.78 in the shrub and tree layers, and that of Trapa japonica was highest as 16.69 in the herb layer. The vegetation type was divided into two groups according to the association analysis. The vegetation of the littoral zone was classified into 7 associations according to the cluster analysis based on the coverage data. These results showed significant differences with those of stand ordination by correspondence analysis based on the species composition and by PCA based on the sediment properties. Factors affecting the distribution of the vascular hydrophytes and hygrophytes were the gradient of particle size, altitude, and water depth.

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Effect of Organic Fertilizers on Growth and Yield of Achyranthes japonica N. (유기질(有機質) 비종(肥種)이 쇠무릎 생육(生育) 및 수량(收量)에 미치는 영향(影響))

  • Kim, Myeong-Seok;Chung, Byeong-Jun;Park, Gyu-Chul;Park, Tae-Dong;Kim, Sang-Chul;Shim, Jae-Han
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.2
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    • pp.131-136
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    • 1998
  • This experiment was carried out to investigate the effect of several organic fertilizers on the growth and root yield of Achyranthes japonica N. from 1995 to 1996. Four organic fertilizers were applied: rice straw manure (RSM), fermented rice straw manure (FRSM), mixed oil cake manure (MOCM) and mighty soil manure (MSM). Organic matter, available phosphate, $K_2O$, CaO, and MgO of soil increased in all plots with the addition of organic fertilizers, specifically with MOCM application. The highest contents of total nitrogen were 6.16% for MOCM and the available phosphate contents were very high in all of organic fertilizers except MSM. RSM and MSM with C/N ratios of 22.5, 17.7, respectively, were easily decomposed but C/N ratios of FRSM and MOCM, which were considered as irresolvable organic fertilizers, were 40.9 and 8.4, respectively. FRSM and MOCM applications increased emergence rate and improved the growth characters of shoot and root parts of plants compared to those of N - P - K fertilization. The highest dry root yield resulted from FRSM 20% and MOCM 26% treatment. There were significantly positive correlations between the growth characters of shoot, root parts of plants and dry root yield in A. japonica grown under organic fertilizer applications.

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A Study of the Analysis of Citizen's Awareness of the Transforming of a Former Military Site into Urban Park - With Special Reference to the City of Euijungbu - (도시 군부대 이전 적지의 공원화 방향에 대한 시민인식 분석 - 의정부시 사례연구 -)

  • Maeng, Chi-Young;Cho, Se-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.2
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    • pp.62-69
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    • 2009
  • This study aimed to analyze citizen awareness of the transforming of a former military site in the city into use as an urban park, and to determine the direction of park creation in the context of urban regeneration. The study focused on the city of Uijungbu, so-called "representative military city" of Korea, which has over eight US military army corps in CBD and which islocated on the northside of Seoul. The questionnaire survey was carried out in the year of 2006, during two months from 1 June till 30 July. The research was carried out by book review as a theoretical base and a questionnaire survey to analyze citizen awareness. The conclusions of this study were as follows. 1. Areas that have been transformed from a former military site to large urban parks for the promotion of economic, social, environmental, and aesthetical value in the context of urban regeneration include Downsview Park(Toronto, Canada), and Great Park(Irvine, CA U.S.A). 2. The citizens of Euijungbu emphasized having casual rest and recreational functions in an urban park, but were also concerned with the promotion of cultural image, activation of the city's economy, etc. 3. The citizens preferred to use the former military site for a park instead of for residential and commercial purposes to introduce cultural facilities and encourage economic activation. 4. All results of this study proposed to use the former military site for encouraging and activating the urban economy, cultural promotion, social reconciliation and aesthetic value by the transforming the site into a large, multi-use park in the context of urban regeneration.

Heavy Metal Contamination of Soils and Stream Sediments at the Sanggok Mine Drainage, Upper Chungju Lake, Korea (충주호 상류, 상곡광산 수계에 분포하는 토양과 하상퇴적물의 중금속 오염)

  • 이현구;이찬희
    • Journal of the Korean Society of Groundwater Environment
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    • v.5 no.1
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    • pp.10-20
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    • 1998
  • Heavy metal contamination in subsurface soils and stream sediments at the Suggok mine area were investigated on the basis of major, trace and rare earth elements geochemistry and mineralogy. The Sanggok mine area is mainly composed of Cambro-Ordovician carbonate rocks. The mine had been mined for Pb-Zn-Fe and Au- Ag, but already closed in past. For major elements, especially Fe (mean value=18.58 wt.%) and Mn (mean value=4. 18 wt.%) are enriched in soils, and the average enrichment indices of soils and sediments are 6.84 and 1.54, respectively. The average enrichment index of rare earth elements are 0.92 of mining drainage sediments and 0.52 of subsurface soils on the tailing dam. Concentrations of minor and/or environmental toxic elements in those samples range from 29 to 3400 for As,1 to 11 for Cd, 35 to 292 for Cu, 50 to 1827 for Pb, 1 to 22 for Sb and 112 to 2644 for Zn. Extremely high concentrations (mean values) are found in subsurface soils on the tailing dam (As=2278, Cd=7, Cu=206, Pb=1372, Sb=14 and Zn=2231 ppm, respectively). Average enrichment index normalized by composition of non-mining drainage sediments is 2.42 in mining drainage sediments and 25.47 in subsurface soils on the tailing dam. Based on EPA value, enrichment index of toxic elements is 0.53 in non-mining drainage sediments, 1.84 in mining drainage sediments and 23.71 in subsurface soils on the tailing dam. As a results from X-ray powder diffraction method, mineral composition of soils and sediments near the mine area varied in part, and are calcite, dolomite, magnesite, quartz, mica, chlorite and clay minerals. With the separation of heavy minerals, soils and sediments of highly concentrated toxic elements included some pyrite, arsenopyrite, sphalerite, galena, goethite and hydroxide minerals on the polished sections.

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Chemical Compositions of the Highway Side Fogwater in Shingal, Kyunggi-Province (경기도 신갈지역 고속도로변 안개의 화학적 조성)

  • 김홍률;주영특;정동준
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.1
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    • pp.11-17
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    • 2003
  • pH value of sampled fogwater at source regions (above highway and road) in Yongin sites showed the lowest value and was increased after passing the forest stands. Changes of ion concentrations through the forest stands showed a lowering tendency at sampling sites. The fogwater passing the forest stands (Quercus mangolica and Pinus rigida) surrendered acid pollutants to crown and stem from the atmosphere. It was concluded that environmental moisture in the atmosphere is acidified in fogwater. The influence was extended to the pure zone, and the frequency of acid rain has increased. The forests are assumed to remove air pollutants because ion concentrations in fogwater decreased after passing the forests. The fogwater which functions as a local sink for pollutants (H$_2$SO$_4$, HNO$_3$, etc.) falling on plant surfaces is considered to effectively remove acid pollutants. But if the deposition of pollutants exceeds the capacity of purification, it would damage the forest ecosystem. Further investigation is necessary to identify tree species tolerant to acid pollutants.

Zooplankton and Neustonic Microplastics in the Surface Layer of Yeosu Coastal Areas (여수 연안 표층에 출현하는 동물플랑크톤과 미세플라스틱)

  • Kang, Hui Seung;Seo, Min Ho;Yang, Yun Seok;Park, Eun-Ok;Yoon, Yang Ho;Kim, Daejin;Jeong, Hyeon Gyeong;Soh, Ho Young
    • Korean Journal of Environmental Biology
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    • v.36 no.1
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    • pp.11-20
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    • 2018
  • In planktonic ecosystems, the microplastics are considered as a potential food source for the zooplankton. To study a relationship between the zooplankton and the neustonic microplastics, a research experiment was carried out during May in the surface layers of the Yeosu coastal areas including Yeoja Bay, Gamak Bay, Yeosuhae Bay, and Botdol Sea. A neustonic zooplankton net (mesh size $300{\mu}m$; mouth area $30cm{\times}18cm$) was towed from the side of the ship in the event that it would not be affected by waves crashing by the ship at a speed of ca. 2.5 knots. All of the microplastic particles were separated from the zooplankton. The zooplankton and microplastics were appearing in a range of 61 to $763indiv.m^{-3}$ and 0.0047 to $0.3471particle\;m^{-2}$, respectively. It was noted that the Acartia omorii, Paracalanus parvus s. l., Labidocera euchaeta, A. hongi, decapod larvae, and cirriped larvae were predominantly seen in the experiment. For verifying relationships between zooplankton and environmental factors in addition to microplastics, a model redundancy analysis (RDA) was performed. The zooplankton were divided into two groups on the basis of feeding types (i.e. particle feeders, and carnivores), and the associated zooplankton larvae were also separately considered. A review of the additional environmental factors such as water temperature, salinity, turbidity, chlorophyll-${\alpha}$ concentration, diatom density, and dinoflagellate density were also contained in the analysis. The results showed that a noted zooplankton abundance had no close relation with the occurring number of microplastic particles, but rather was significantly related with other noted environmental factors such as temperature, salinity, turbidity, and chlorophyll-${\alpha}$ concentration. This fact implies that most zooplankton can feed themselves as a unit, by selecting the most likely available nutritious foods, rather than microplastics under the circumstance of food-richness areas, such what food resources are available as in the location of coastal waters.