• Title/Summary/Keyword: $C^*$-envelopes

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Isolation and Characterization of PERV-C env from Domestic Pig in Korea

  • Park, Sung-Han;Bae, Eun-Hye;Park, Sang-Min;Park, Jin-Woo;Lim, Mi-Suk;Jung, Yong-Tae
    • Journal of Microbiology and Biotechnology
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    • v.18 no.10
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    • pp.1735-1740
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    • 2008
  • Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.

Fine Structure of Oocyte Envelopes of Diploid and Triploid Biotypes in Cobitis hankugensis-Iksookimia longicorpa Complex (Cobitis hankugensis-Iksookimia longicorpa Complex의 2배체, 3배체집단의 난막 미세구조)

  • Ko, Meong-Hun;Park, Jong-Young
    • Korean Journal of Ichthyology
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    • v.22 no.1
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    • pp.56-60
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    • 2010
  • The Cobitis hankugensis-Iksookimia longicorpa complex from Korea has been presumed to be a unisexual lineage, originating from C. hankugensis and I. longicorpa and having almost all females. Recently, it was confirmed that the complex consisted of one diploid and two triploid complexes. From observation of their oocyte envelopes, three forms could be classified: a villous projection in I. longicorpa, a granular one in C. hankugensis and two triploid complexes, and a granule with villous one in the diploid complex. Even within the same granular projection, they showed specific features in length and density number from each other. These architectures are first observed in cobitid complexes and may playa role in identification of diploids and triploids.

An Experimental Study on Ventilation and Thermal Performance of Passive Ventilation Building Envelopes (패시브환기외피의 통기 및 열성능에 관한 실험적 연구)

  • Yoon, Seong-Hwan;Lee, Tae-Cheol;Kang, Jung-Sik
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.23 no.11
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    • pp.711-717
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    • 2011
  • In this study, 5 types of PVS(Passive ventilation system) units are made and experimented its ventilation performance, thermal performance according to open rate and hole diameter of perforated aluminum plane. Results are as follows. 1) The ventilation performance increases approximately 50~70% according by the open rate of PVS increasing. Also, the ventilation performance increases about 2%~12% according by the hole diameter of PVS increasing. 2) In winter temperature/pressure condition(in : $20^{\circ}C$, out : $-2^{\circ}C/{\Delta}P$ : 0.2~5.0Pa) the temperature of inflow air decreases according by the open rate of PVS increasing. Heat gain performance decreases 10.1%, 25.6% when open rate increases 3) In the same condition, Heat gain performance decreases 18.3%, 18.8% according by the hole diameter of PVS increasing.

Isotopic Compositions of Ruthenium Predicted from Stellar Evolution Using the NuGrid Project

  • Kim, Seonho;Sung, Kwang Hyun;Kwak, Kyujin
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.46.2-46.2
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    • 2021
  • Presolar silicon carbide (SiC) grains form around in the envelopes of asymptotic giant branch (AGB) stars by satisfying C/O>1 which is an optimal condition for SiC grains to condense in the stellar outflows. Ruthenium (Ru) isotopes are locked into the SiC grains during the condensation of SiC grains. We investigate the isotopic compositions of Ru in the stellar winds by using the NuGrid data, which are obtained by nucleosynthesis calculations during the stellar evolution. We compare the isotopic compositions of Ru obtained from the NuGrid data with measurements and the predictions obtained from different codes. Our results present a piece of evidence that SiC grains in the presolar system came from low-mass and low-metallicity AGB stars, also confirming that they were not from massive stars. We also suggest a new scenario in which the total stellar yields are also considered because SiC grains can condense during the collapse of molecular clouds.

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Micromechanical failure analysis of composite materials subjected to biaxial and off-axis loading

  • Ahmadi, Isa
    • Structural Engineering and Mechanics
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    • v.62 no.1
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    • pp.43-54
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    • 2017
  • In this study, the failure behavior of composite material in the biaxial and off-axis loading is studied based on a computational micromechanical model. The model is developed so that the combination of mechanical and thermal loading conditions can be considered in the analysis. The modified generalized plane strain assumption of the theory of elasticity is used for formulation of the micromechanical modeling of the problem. A truly meshless method is employed to solve the governing equation and predict the distribution of micro-stresses in the selected RVE of composite. The fiber matrix interface is assumed to be perfect until the interface failure occurs. The biaxial and off-axis loading of the SiC/Ti and Kevlar/Epoxy composite is studied. The failure envelopes of SiC/Ti and Kevlar/Epoxy composite in off-axis loading, biaxial transverse-transverse and axial-transverse loading are predicted based on the micromechanical approach. Various failure criteria are considered for fiber, matrix and fiber-matrix interface. Comparison of results with the available results in the litreture shows excellent agreement with experimental studies.

A Simple Method for Assessing Severity of Common Root Rot on Barley

  • Arabi, Mohammad Imad Eddin;Jawhar, Mohammad
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.451-453
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    • 2013
  • Common root rot caused by Cochliobolus sativus is a serious disease of barley. A simple and reliable method for assessing this disease would enhance our capacity in identifying resistance sources and developing resistant barley cultivars. In searching for such a method, a conidial suspension of C. sativus was dropped onto sterilized elongated subcrown internodes and incubated in sandwich filter paper using polyethylene transparent envelopes. Initial disease symptoms were easily detected after 48h of inoculation. Highly significant correlation coefficients were found in each experiment (A, B and C) between sandwich filter paper and seedling assays, indicating that this testing procedure was reliable. The method presented facilitates a rapid pre-selection under uniform conditions which is of importance from a breeder's point of view.

Strengthening of deficient RC joints with diagonally placed external C-FRP ropes

  • Karayannis, Chris G.;Golias, Emmanouil
    • Earthquakes and Structures
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    • v.20 no.1
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    • pp.123-132
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    • 2021
  • Deficient beam-column joints of reinforced concrete (RC) structures designed to older practices and codes often lead to destructive local or global failures. A strengthening technique for these joints based on the use of the new and innovative Carbon-FRP (C-FRP) ropes is presented and investigated. The C-FRP ropes are diagonally placed in superficial notches on the two sides of the joint. Two full scale external substandard joint subassemblages with the same characteristics, one unstrengthened and one strengthened with diagonally applied C-FRP ropes, are constructed and tested in cyclic loading. Special attention has been given to the elaboration of the acquired test measurements. The extracted conclusions are based on the comparative study of the hysteretic responses of the specimens, the observed maximum load envelopes, the comparisons of the joint body shear deformations as measured using diagonally mounted LVTDs, the calculated nominal principal stresses developed in the joint regions, the assessed joint damage as expressed by the damage index by Park and Ang and finally the calculated values of the equivalent damping ratio. From these comparisons it is concluded that application of diagonally mounted C-FRP ropes on the two sides of the joint body of exterior connections is an efficient easy-to-apply technique for the strengthening of substandard RC joints.

Study on Morphological Changes and TUNEL Reaction of Apoptotic Cells in Mouse Liver by Apoptosis Induction

  • Kwak, Soo-Dong;Yang, Je-Hoon;Koh, Phil-Ok;Seo, Deuk-Lok;Kang, Chung-Boo
    • Biomedical Science Letters
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    • v.8 no.3
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    • pp.179-184
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    • 2002
  • We investigated the morphological changes and TUNEL reaction of apoptotic cells in the liver of D-galactosamine (20 mg/mouse) and lipopolysaccharide (5 $\mu\textrm{g}$/mouse)-treated 30 mice (BALB/c), and in additioa also of apoptotic cells in kidney and spleen. The livers and other some organs of mice at 6, 12, 24, 48 and 72 hrs after treatment were collected and were fixed with 10% neutral formalin and paraffin sections were stained with hematoxylin-eosin or terminal deoxynucleotidly transferase-mediated dUTP nick end labeling (TUNEL) method. Morphological changes in apoptotic hepatocytes were chondensation of nuclei and density of cytoplasms, then the margination and pyknosis of chromatin, the formation of half-moon- or horse-shoe- or ship-like shapes of condensed chromatin mass, lastly formation of apoptotic bodies, disappearance of nuclear envelopes, decrease of stainability, then lysis and disappearance of apoptotic bodies. TUNEL positive reactions of hepatocytes were appeared first moderate in uncondensed hepatocytes, severe in condensed hepatocytes, moderate in chromatin-marginated hepatocytes. These reactions also were appeared moderate in hepatocytes with half-moon- or horse-shoe- or ship-like pyknotic chromatin mass or apoptotic bodies, and mild or negative in hepatocytes with lysed apoptotic bodies or with disappeared nuclear envelopes. Consequently these results suggested that TUNEL positive reactions of hepatocytes appeared at more early stages than appearance of chromatin condensation and disappeared at more early stage than disappearance of histological findings of apoptosis. We also confirmed that the differentiation of apoptotic cells from normal healthy cells of Kupffer cells and vascular endothelial cells in liver, reticular cells and lymphocytes in spleen and epithelial cells of tubules and ducts in kidney was impossible in H-E preparations but was possible in TUNEL preparations.

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New records of the genus Cyanobium and Cyanobium gracile (Synechococcales, Cyanophyceae) in Korean freshwater

  • Kwon, Dae Ryul;Jo, Bok Yeon;Jang, Seok Won;Lee, Chang Soo;Nam, Seung Won
    • Korean Journal of Environmental Biology
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    • v.39 no.1
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    • pp.32-38
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    • 2021
  • Cyanobium is a genus of picoprokaryotic cyanophytes, which includes species worldwide. The present study investigated the morphology, ultrastructure, and molecular phylogeny of the unrecorded genus Cyanobium Rippka & Cohen-Bazire 1983 and species Cyanobium gracile Rippka & Cohen-Bazire 1983. A C. gracile culture from a freshwater sample collected from the Adongji pond was established by single-cell isolation. Morphological data were analyzed using light and transmission electron microscopy. C. gracile lives as solitary cells without gelatinous envelopes and is ovate, oval, or shortly rod-shaped. Thylakoids are laid along the cell walls, with three thylakoid membranes parallel to each other. Nucleoplasm was observed in the center of the cell. Molecular phylogeny performed with data from 16S small subunit ribosomal DNA gene (SSU rDNA) sequences showed that the three strains of C. gracile, including the type strain (PCC6307) and a newly recorded strain (Adong101619), formed a distinct clade with a high supporting value (maximum-likelihood=100, pp=1.00). Based on morphology and molecular data, we report the newly recorded C. gracile in Korea.