• Korean Journal of Microbiology
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• v.52 no.2
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• pp.230-235
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• 2016

This study was carried out to analyze the differences in enzyme activity and stability between the native Fe superoxide dismutase (FeSOD) and the 6xHis-tagged superoxide dismutase (6xHis-FeSOD) of Streptomyces subrutilus P5. The optimum pHs for both native FeSOD and 6xHis-FeSOD were 7, while the pH range of the activity was narrower for the 6xHis-FeSOD. The native FeSOD was stable at pH 4-9, but the 6xHis-FeSOD lost its stability at pH > 9. The temperatures of the optimum activities were same for both types of enzymes. However, the heat stability of the 6xHis-FeSOD was clearly decreased; even at $20^{\circ}C$ the enzyme lost the activity after 360 min. In contrast, the native FeSOD was stable after 720 min at below $40^{\circ}C$. $H_2O_2$ inhibition was occurred already at 0.5 mM for the 6xHis-tagged enzyme. Therefore, from the results that the 6xHis-FeSOD retained the enzyme activity at pH 6-7 and $20-40^{\circ}C$, it can be assumed that the protein structure became destabilized under different storage conditions and sensitive to the enzyme inhibitor.

• The Journal of the Acoustical Society of Korea
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• v.16 no.4
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• pp.94-100
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• 1997

In order to investigate the radiation of sound from the King Song-Duk bell, we measured the sound pressure around the bell at every 30$^{\circ}$ using a microphone line array which was composed of 30 microphones separated by 15cm;total number of measurement point was 360. The sound field was estimated by using cylindrical acoustic holography. The spectrum of measured sound pressure demonstrates that it is almost like white noise in the very beginning, but in 10 seconds two close frequency components(64.06Hz, 64.38Hz) remain and make a famous beating. This beating sound is often believed to make unique sound of the King Song-Duk bell. The mode shapes of that frequency components are the same except that one is rotated by 45$^{\circ}$ from the other. This phenomenon occurs at the other pairs of components are the same except ones in very high frequency range where the mode shapes are rather complex.

• Journal of Applied Biological Chemistry
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• v.53 no.2
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• pp.71-76
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• 2010

A gene encoding the mannanase of Bacillus licheniformis WL-12, which had been isolated from Korean soybean paste, was cloned into Escherichia coli and nucleotide sequence of the mannanase gene was subsequently determined. The mannanase gene consisted of 1,080 nucleotides encoding a polypeptide of 360 amino acid residues. The deduced amino acid sequence was identical to that of putative mannanase from B. liceniformis DSM13 belonging to GH family 26. The mannanase was partially purified from cell-free extract of the recombinant Escherichia coli carrying a WL-12 mannanase gene by ammonium sulfate fractionation and DEAE-Sepharose column chromatography. Optimal conditions for the partially purified enzyme occurred at pH 6.0 and $65^{\circ}C$. The enzyme showed higher activity on locust bean gum (LBG) galactomannan and konjac glucomannan than on guar gum galactomannan. The predominant products resulting from the mannanase hydrolysis were mannose, mannobiose and mannotriose for LBG or mannooligosaccharides. The enzyme could hydrolyze mannooligosaccharides larger than mannobiose.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2016.05a
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• pp.98-99
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• 2016

The aim of study is to develop a dual driven ship berthing/deberthing system with $360^{\circ}$ steerability. A large ship used bow thruster, side thruster and pod propulsor etc. when approaching to a pier. But as marine leisure boats become large-scaled, the number of accidents is recently increasing what caused a ship to crash into a ship and a ship to crash into mooring facilities during berthing/deberthing on a marina. To solve the problem, the control responsiveness of a joystick connected with two motors and a propeller was checked and torque was increased by the electromagnetic design of magnetic gear. A sea trial test was carried out to investigate a performance of the developed system in the real sea.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.11
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• pp.1433-1439
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• 2012

This paper introduces a sensor system based on indoor locations in order to implement the Building Energy Management System. This system consists of a thermopile sensor and an ultrasonic sensor. The sensor module is rotated by $360^{\circ}$ and yawed up-and-down by two electric motors. Therefore, it can simultaneously detect the number and location of the inhabitants in the room. It uses wireless technology to communicate with the building manager or the smart-home server, and it can save electric energy by controlling the lighting system or heating/air conditioning equipment automatically. We also demonstrate the usefulness of the proposed system by applying it to a real environment.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.5
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• pp.1080-1088
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• 2004

In this paper, the interesting moving objects tracking algorithm using color information on Multi-Video camera against variance of intensity, shape and background is proposed. Moving objects are detected by using difference image method and integral projection method to background image and objects image only with hue area, after converting RGB color coordination of image which is input from multi-video camera into HSI color coordination. Hue information of the detected moving area are segmented to 24 levels from $0^{\circ}$ to $360^{\circ}$. It is used to the feature parameter of the moving objects that are three segmented hue levels with the highest distribution and difference among three segmented hue levels. To examine propriety of the proposed method, human images with variance of intensity and shape and human images with variance of intensity, shape and background are targeted for moving objects. As surveillance results of the interesting human, hue distribution level variation of the detected interesting human at each camera is under 2 level, and it is confirmed that the interesting human is tracked and surveilled by using feature parameters at cameras, automatically.

• Korean Journal of Materials Research
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• v.9 no.4
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• pp.378-385
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• 1999

To evaluate the effect of Sn on the corrosion behavior of Zr alloys for nuclear fuel claddings, the corrosion tests on the binary Zr-xSn and the ternary Zr-0.4Nb-xSn alloys were performed in water at $360^{\circ}C$. The binary alloys containing 0.5, 0.8 and 1.5wt.% Sn showed the transition corrosion rate at 15 days. On the other hand, the binary alloy containing 2.0wt.% Sn showed a good corrosion resistance without the transition of corrosion rate up to 80 days. The corrosion rate of the ternary alloy increased with increasing Sn content. The difference of corrosion behaviors between binary and ternary alloys is considered due to the different solubility of Sn, Nb content and precipitates. The corrosions of Zr-xSn and Zr-0.4Nb-xSn alloys would be controlled by the fraction of tetragonal-$ZrO_2$and the amount of hydrogen pick-up.

• Journal of Convergence for Information Technology
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• v.7 no.3
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• pp.111-116
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• 2017

This study offers the possibility as a new product of souvenirs from cultural tourist sites with virtual reality contents, instead of a simple exhibit. Users can watch augmented reality contents on souvenirs, and use the virtual experience service of cultural tourist sites with VR viewer. It has 4K-class high-quality $360^{\circ}$ image capture to increase the immersion of users in virtual space, implement virtual reality space and intuitive UI interaction with users using IMU (Inertial Measurement Unit). It is also possible to apply various add-on functions, including 3D contents, through technical development to increase its marketability, and apply in other industries. In addition, the prototype of the souvenir and VR viewer will be made by using a 3D printer and such in order to apply the contents which will be introduced in this study. Various product expansions can be considered through consultation with the relevant companies for the use of ready-made products.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.909-916
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• 2013

Concrete containing lightly burnt MgO has long term expansibility. It also could compensate for the thermal shrinkage of mass concrete, because the hydration of MgO proceeds at a slow pace to long-term age. Thus, lightly burnt MgO has been applied to the construction of mass concrete such as dams. Recently, the expansion characteristics of MgO concrete with fly ash that could be applied to mass concrete for the reduction of hydration heat have been studied and however, limited studies on its durability. This study investigates the long-term durability characteristics of fly ash concrete with lightly burnt MgO. The durability tests on carbonation, freezing-thawing, diffusion of chloride, and resistance to sulfate attack were carried out for MgO concrete with curing for 360 days in submerged condition with different temperature of 20 and $50^{\circ}C$. The results reveal that MgO concrete shows a greater resistance of carbonation, diffusion of chloride, and resistance to sulfate attack. On the other hand the resistance of freezing-thawing was little influenced by MgO powder.

• Korean Journal of Materials Research
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• v.9 no.5
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• pp.452-459
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• 1999

To develop advanced cladding materials, the effect of Nb addition on the microstructure and corrosion characteristics of Zr-0.8Sn-xNb alloys was investigated. As the Nb content increased, the grain size decreased and the volume fraction of precipitates increased. It was observed from the corrosion test at $360^{\circ}C$ that the corrosion resistance increased with decreasing Nb content. The best corrosion resistance was obtained in Zr-0.8Sn-0.2Nb alloy with high volume fraction of tetra-$ZrO_2$in the oxide. Therefore, it is suggested that Nb in theZr-0.85Sn-xNb system should be added within the solubility limit of Nb from the viewpoint of alloy design.