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Analysis of Catechin Contents in Commerical Green Tea By HPLC (시판 녹차중 카테킨의 함량 분석)

  • 최성희;이병호;최홍대
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.4
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    • pp.386-389
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    • 1992
  • The four main tea catechin components (-)-epicatechin (EC), (-)-epigallocatechin (EGC) , (-)-epicatechin gallate (ECg), and (-)-epigallocatechin gallate (EGCg) were analyzed quantitatively from commerical green teas by HPLC. CATechin of the most amounts in steamed and parched teas was EGcg (steamed 1st : 7.54% , parched 1st : 7.88%). Amounts of catechins decreased in the following order : EGCg > EGC>ECg>EC. Almost same tendency of catechin components change of 1st tea and 2nd tea differed to harvesting time being observed in steamed and parched teas. In 2nd tea, amounts of EGCg increased more than in 1st tea. It seems that this change effects on the quality of tea taste.

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Tumorigenesis of Transgenic Mice Induced by Mouse Vasopressin-SV40 T Hybrid Oncogene

  • Lee, Eun-Ju;Kim, Myoung-Ok;Kim, Sung-Hyun;Park, Jun-Hong;Park, Jung-Ok;Cho, Kyong-In;Park, Hum-Dai;Ryoo, Zae-Young
    • Proceedings of the KSAR Conference
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    • 2002.06a
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    • pp.92-92
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    • 2002
  • The neuropeptide vasopressin (VP) is a nine- amino acid hormone synthesized as preprohormone in the cell bodies of hypothalamic magnocellular neurons. The tumor in magnocellular neurons of the hypothalamus is associated with disfunctions of the cell bodies, leading to the diabetes insipidus. In order to produce the disease models with a defect in VP synthesis and its secretion, we have produced the transgenic mice regulated by VP constructs containing 3.8 kbp of the 5'flanking region and all the exons and introns in the mouse VP gene, which was fused at the end of exon 3 to a SV40 Tag. The two VP-transgene constructs differed by the lengths of their VP gene 3' flanking regions (2.1 versus 3.6 kbp). (omitted)

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A New Receptor for site Clonidine in the Eel, Anguilla japonica Intestine (뱀장어(Anguilla japonica)장의 상피세포막에 존재하는 새로운 clonidine 결합 수용체에 관한 연구)

  • Kim, Hung-Tae;Seo, Jung-Soo;Park, Nam-Gyu;Lee, Hyung-Ho;Chung, Joon-Ki
    • Journal of fish pathology
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    • v.14 no.1
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    • pp.31-36
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    • 2001
  • A novel clonidine binding sites were characterized in the intestinal membrane isolated from seawater eels, Anguilla japonica. The specific clonidine binding sites consisted of at least two classes, high affinity ($K_d=1.4{\pm}0.3$ nM n = 5) and low affinity ($K_d=175{\pm}34$ nM n = 5) sites. The specific binding of 2 nM [$^3H$]clonidine was most enhanced at $20^{\circ}C$ and pH 7.5, and reversed by unlabelled clonidine. Such binding was hardly inhibited by adrenaline, yohimbine or rauwolscine, indicating that most binding sites are distinct from $\alpha_2$-adrenoceptor. The specific clonidine binding sites was inhibited by various imidazoline/guanidinium drugs, indicating existence of imidazoline/guanidinium receptive sites (IGRS) or imidazoline receptors in the eel intestine. Competition experiments revealed that rank order to displace 2 nM [$^3H$]clonidine from their binding sites was as follows : guanabenz > cirazoline = naphazoline = UK14,304 = ST587 $\geq$ clonidine $\geq$ idazoxan = RX821002 = tolazoline > ST93 = oxymetazoline = amiloride = ST91 > yohimbine = efaroxan = rauwolscine $\geq$ adrenaline = ST567 = histamine = agmatine. Although physiological role of IGRS is not clear yet even in mammalian cell/tissues, eel intestine may be a good model to elucidate how the IGRS act in the cell and to decide what is the endogenous ligand for the IGRS.

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Species Composition of Benthic Macroinvertebrates and Water Evaluation Using Their Species in the Songji River in Korea (한국 송지천에서 저서성대형무척추동물의 종조성과 이를 이용한 수질 평가)

  • Lee, Byeong Ryong;Huh, Man Kyu
    • Journal of Life Science
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    • v.29 no.5
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    • pp.580-587
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    • 2019
  • Benthic macroinvertebrates were analyzed in March, June, September, and December 2018 to evaluate water quality in the Songji River in Sacheon-ci, Korea. The identified benthic macroinvertebrates included 447 individuals belonging to 20 species, 18 families, 12 orders, 5 classes, and 3 phyla. Various ecological parameters were estimated for evaluation of the river status. The total ecological score of benthic macroinvertebrate community (TESB) varied from 17 (Station D) to 41 (Station A). The saprobic index and ecological score of benthic macroinvertebrate community (ESB) for the evaluation of river status revealed a water quality evaluation at Station A of II (oligosaprobic), indicating some satisfactory water protection. The benthic macroinvertebrate index (BMI) varied from 25.207 (Site C) to 39.348 (Station A). The evaluation of the river status at Stations C and D was polysaprobic, and sensitive taxa were absent. The mean Shannon-Weaver index (H') of diversity varied from 1.288 (Station D) to 2.250 (Station A). The classification of saprobity based on H' was ${\beta}$-mesosaprobic at Station A and ${\alpha}$-mesosaprobic at the other stations. The value of geometric density was varied from 1.229 (Station A) to 2.071 (Station D), with a mean of 1.582. An artificial load is being added to this river. One of load is the rectal river construction which flows straight through the river physics. Thus, the environment of living organisms deteriorates due to insufficient water. In order to secure the quality of the Songji River and a good environmental habitat, several low-height stepped-beam structures are required.

A Study on Low Concentration Substrate Removal by Using the Aerated Submerged Biofilter (호기 생물막법에 의한 저농도 기질제거법에 관한 고찰)

  • 홍성철;정문식
    • Journal of Environmental Health Sciences
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    • v.15 no.2
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    • pp.59-68
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    • 1989
  • This study was performed employing the two stage aerated submerged biofilter of media pore size 1.5cm and 2cm, and infiuent substrate concentrations were 30.25mg COD/l, 50.1mg COD/l respectively. The purpose was to determine the treatment efficiency at the low concentration infiuent, reaction order and substrate flux with application of variable-order model that was presented by Rittmann and McCarty. . The results are as follows. 1. Treatment efficiency of 1st reactor was about BOD 82% and COD 76%, when effluent concentration was BOD 3.9 ~ 6.8, COD 7.1 ~ 12.5 mg/l, and this effluent concentration didn't satisfy the water quality grade I, II of river and lake. But as treated effluent of 1st reactor with 2nd reactor, we could achieve appropriate water quality, since instillation of 2nd reactor was needed. 2. Difference of media pore size between 1.5cm and 2cm didn't effect significantly to treatment efficiency and since this of 2nd reactor was about BOD 60%, COD 50%, an consideration of economic point of view should be carried out in field application. 3. Reaction order and substrate flux was varied 0.9851~0.9956 and 0.0028~0.0405 mg/$cm^{2} \cdot day$, and the substrate flux was increased as infiuent substrate concentration increased.

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Backstepping Control of Output-feedback Systems Using Cascade Observer (축차 관측기를 이용한 출력 피이드백 시스템의 백스테핑 제어)

  • Kim, Eung-Seok;Kim, Dong-Hun;Hyun, Keun-Ho;Kim, Hong-Pil;Yang, Hai-Won
    • Proceedings of the KIEE Conference
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    • 2001.07d
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    • pp.2237-2239
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    • 2001
  • Backstepping procedures for output-feedback of nonlinear systems are considered. For these systems, a sliding mode cascade observer to estimate derivatives of the output is builded. In the 1st step of the observer, the output is estimated, and the 1st order derivative of the output is estimated via the 2nd step of the observer. Also, n-th order derivative of the output is estimated in the n+1th step of the observer. Observer errors and tracking errors converge to a residual set close to zero asymptotically.

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Passenger & Car Carrier's Superstructure Vibration induced by the 1st order Excitation of Whirling Vibration on the Propulsion Shafting System (추진축계 횡진동 1차 기진력에 의한 여객.차도선의 선체 상부진동)

  • Lee, D.C.;Ko, J.Y.;Kim, J.R.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2000.11a
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    • pp.301-306
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    • 2000
  • Small passenger and car carrier ships are mainly used as a connection way between land and small islands. And these ships should be designed to enlarge the capacity of passenger and car loading within limited space and draft. So the resonances of various vibrations exist in normal operation range of engine and propulsion shafting. In this paper, hull's superstructure vibration which was especially induced by the 1st order excitation of whirling vibration on the 2-engines and 2-propulsion shafting systems is introduced. Also these are verified via the theoretical analysis using transfer matrix method and FEA software ANSYS and its vibration measurement.

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Taste Components of Soy Sauce Manufactured by Bacillus Species SSA3-2M1 and Fused ST723-F31

  • Kim, Haeng Ja;Eun Ju Lee;Ok Sun Shin;Myeong Rak Choi;Jong Kyu Kim
    • Journal of Microbiology and Biotechnology
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    • v.6 no.3
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    • pp.202-208
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    • 1996
  • In order to investigate fermenting conditions and the microorganisms necessary for factory production of traditional Korean soy sauce, we manufactured soy sauce made by Bacillus species SSA3-2M1 and fused ST723-F31 with aeration (1/30 vvm, 113 vvm and 2/3 vvm) at $30^{\circ}C$ for 40 days. This method was chosen to investigate the changes of dissolved oxygen, pH, cell number, flavor and the taste components during fermentation. When air was supplied (2/3 vvm) to the fermentor during fermentation, the flavor of the soy sauce and the composition of taste components (free amino acids, free sugars and organic acids) were similar to that of traditional Korean soy sauce after 22 days. The results of our experiments indicates that the mass production of traditional Korean soy sauce is possible using Bacillus species SSA3-2M1 and fused ST723-F31 given sufficient aeration.

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Analytical and Experimental Studies on the Natural Frequency of a Composite Train Carbody (복합재 철도차량 차체 고유진동수에 대한 해석 및 시험적 연구)

  • Jeong Jong-Cheol;Cho Sea-Hyun;Seo Seong-Il;Kim Jung-Seok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.30 no.4 s.247
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    • pp.473-480
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    • 2006
  • This paper explains analytical and experimental studies to evaluate the natural frequency of a composite carbody of Korean tilting train. The composite carbody with length of 23m was manufactured as a sandwich structure composed of 40mm-thick aluminium honeycomb core and 5mm-thick woven fabric carbon/epoxy face. From the finite element analysis, the 1st bending and 1st twisting natural frequency of the composite carbody were 11.67Hz and 14.4Hz, respectively. In order to verify the analytical results, the natural frequency measuring tests were performed. The measured 1st bending and twisting natural frequencies of the composite carbody were 10.25Hz and 11.0Hz, respectively. Both of these results satisfied the design requirement.

Sciatic Neuropathy after Intramuscular Injection at a Site Remote from the Nerve (신경과 먼 부위 근육 내 주사 후 발생한 좌골신경병증)

  • Yun, Soo In;Park, Jisoo;Ko, Yun Dam;Song, Dae-Heon;Park, Jihye
    • Clinical Pain
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    • v.20 no.1
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    • pp.43-48
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    • 2021
  • Sciatic nerve can be injured by various mechanism such as compression, traction during surgery, and direct trauma. This case reports a sciatic neuropathy caused by compression due to hematoma occurring after intramuscular injection in the gluteus medius muscle far from the nerve. In order to avoid occurrence of sciatic neuropathy after buttock injection, the injection was made in the upper outer quadrant of the buttock, but sciatic neuropathy occurred. Sciatic neuropathy can be confused with lumbar radiculopathy, so differential diagnosis is important.