• Journal of Food Hygiene and Safety
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• v.13 no.4
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• pp.383-387
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• 1998

This study was undertaken with packed tofu products to serve as a basic source for sanitary control of tofu production by detecting spoilage bacteria in tofu from which are isolated, investigating heat-resistance and growth characteristics of spoilage bacteria. Isolated strains were confirmed as relevant strains in tofu spoilage, and Strain No. Tl, T2 show 92% probability to be Enterobacter amnigenus, and 96% to be Flavobacterium indologenes according to the result of identifYing strains by using Vitek system. Both strains had high viability at $35^{\circ}C$, pH 6.5. In the heat-resistance test of isolated strains, Enterobacter amnigenus Tl was treated for 2 mins, the number remained 56.3% of the initial number at $60^{\circ}C$, 37.8% at $70^{\circ}C$, 34.0% at $80^{\circ}C$ and 22.2% at $90^{\circ}C$, and Flavobacterium indologenes T2 was treated for 2 mins, the number remained 74.8% of the initial number at $60^{\circ}C$, 65.7% at $70^{\circ}C$, 37.8% at $80^{\circ}C$ and 9.3% at 90^{\circ}C$.

• Journal of Life Science
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• v.28 no.6
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• pp.639-647
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• 2018

A new heat shock protein 70 was identified in red-spotted grouper (Epinephelus akaara) based on an expression analysis. The cDNA of red-spotted grouper Hsp70 (designated RgHsp70) was cloned by the rapid amplification of cDNA ends (RACE) techniques. The full-length of RgHsp70 cDNA was 2,152 bp, consisting of a 5'-terminal untranslated region (UTR) of 105 bp, a 3'-terminal UTR of 274 bp, and an open reading frame (ORF) of 1,773 bp that encode a polypeptide of 590 amino acids with a theoretical molecular weight of 64.9 kDa and an estimated isoelectric point of 5.2. Multiple alignment and phylogenetic analyses revealed that the RgHsp70 gene shares a high similarity with other Hsp70 fish genes. RgHsp70 contained all three classical Hsp70 family signatures. The results indicated the RgHsp70 is a member of the heat shock protein 70 family. RgHsp70 mRNA was predominately expressed in the liver, with reduced expression noted in the head-kidney tissues. The expression analysis of different water temperatures (21, 18, 15 and $12^{\circ}C$) for sampled livers revealed that expression gradually increased at $12^{\circ}C$ compared to $21^{\circ}C$. In this study, the effects of water temperature lowering on the physiological conditions were investigated, and the results revealed that novel RgHsp70 may be an important molecule involved in stress responses.

• Solar Energy
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• v.9 no.1
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• pp.70-77
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• 1989

Sodium pyrophosphate that melting point is $79-80^{\circ}C$ have been Studied on heat storage and heat discharge. In heat storage process, sodium pyrophosphate was kept up initial temperature $50^{\circ}C,\;60^{\circ}C,\;70^{\circ}C$ which melt by heated water at temperature $85^{\circ}C,\;90^{\circ}C,\;95^{\circ}C$. In heat discharge process, initial temperature of sodium pyrophosphate was maintained at temperature $85^{\circ}C,\;90^{\circ}C,\;95^{\circ}C$ which varied cooling temperature $50^{\circ}C,\;60^{\circ}C,\;70^{\circ}C$. The experiment has been reached conclusions as follows. 1) Heat transfer properties of phase change material is controlled by conduction during heating and cooling process. 2) The temperature increased rapidly at initial stage and transient region increase slowly because of characteristic of latent heat. 3) The lower cooling water temperature is the less the time that get to thermal equivalent state take during discharge process. 4) The higher cooling water temperature is the less temperature difference between top and bottom in P.C.M during discharge process.

• Korean Journal of Environmental Biology
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• v.33 no.4
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• pp.450-458
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• 2015

The heat shock proteins (Hsps), one of the most highly conserved groups of proteins, play crucial roles in protecting cells against environmental stressors, such as temperature, salinity, heavy metals and pathogenic bacteria. The glutathione S-transferases (GST) have important role in detoxification of oxidative damage, environmental chemicals and environmental stress. The purpose of this study is to investigate the gene expression of Hsp70 and GST on change of temperature and salinity in Mytilus coruscus. The M. coruscus was cultured in incubator of separate temperature and salinity (8, 20, $30^{\circ}C{\times}20$‰, 25‰, 30‰) for 28 days. Ten individuals in each group were selected after each 14 and 28 days exposure. Results that the expression of Hsp70 mRNA was no significant changed in M. coruscus exposed to temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$) and salinity (20‰, 25‰, 30‰) for 14 days. Whereas the expression of Hsp70 mRNA was increased in exposure to temperature $30^{\circ}C$ and salinity (20‰, 25‰, 30‰) for 28 days. The expression of GST mRNA was increased in exposure to temperature $30^{\circ}C$, salinity (25‰, 30‰) for 14 days and temperature ($8^{\circ}C$, $20^{\circ}C$, $30^{\circ}C$), salinity (20‰, 25‰, 30‰) for 28 days. These results suggest that Hsp70 and GST were played roles in biomarker gene on the thermal and salinity stress.

• Journal of Power System Engineering
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• v.3 no.2
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• pp.51-56
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• 1999

Fatigue crack propagation rates and characteristics of the SA516-70 steel which is used for the low temperature pressure vessels, were studied in the room temperature of $10^{\circ}C$ and low temperature ranges of $-10^{\circ}C,\;-30^{\circ}C,\;-50^{\circ}C,\;and\;-70^{\circ}C$ with stress ratio of R=0.05. The obtained experimental results are as follows; 1) In the logarithmic relationship between the fatigue crack propagation rate(da/dN) and stress intensity factor K, the linear relationship was obtained up to da/dN > $8{\times}10^3$ mm/cycle in the same of room temperature, but in low temperature case, the relationship was extended to the range of low crack propagation rate. 2) The lower limit stress intensity factor of SA516-70 ${\Delta}K_{th}\;was\;23MPa\sqrt{m}$ and in the case of low temperature $-50^{\circ}C\;and\;-70^{\circ}C$, the crack propagation rate da/dN which showed a linear relation, reached rapidly to the ${\Delta}K_{th}$. As the results, the crack propagation rates of $-50^{\circ}C\;and\;-70^{\circ}C$ were lower than that of room temperature and according to the testing temperature the rates were decreased rapidly to the ${\Delta}K_{th}$. 3) On the relationship between the stress intensity factor ${\Delta}K$ and the track propagation cycle, the stress intensity factors of low cycle region was rapidly increased at low temperature, but ${\Delta}K$ was increased rapidly at room temperature of high cycle. 4) On the relationship between the fatigue crack propagation rate and cycle, the fatigue crack propagation rate showed higher gradient in the room temperature than the low temperature due to the increment in ductility at low temperature.

• Physical Therapy Korea
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• v.17 no.1
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• pp.1-8
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• 2010

The purpose of this study was to compare EMG activity for pectoralis major muscle during shoulder movement with various abduction angle and rotation position in supine position. Fifteen healthy subjects were recruited for this study. All subjects performed shoulder horizontal adduction holding a 2 kg dumbbell in shoulder abduction $40^{\circ}C$, $70^{\circ}C$, $90^{\circ}C$, $130^{\circ}C$, $160^{\circ}C$ with shoulder neutral, internal rotation (IR), and external rotation (ER). Surface EMG activity was recorded from pectoralis major clavicle part and pectoralis major sternum part for 5 seconds and EMG activity was normalized to the value of maximal voluntary isometric contraction (%MVIC). Dependent variables were examined with 3 (Neutral, IR, ER) ${\times}$ 5 ($40^{\circ}C$, $70^{\circ}C$, $90^{\circ}C$, $130^{\circ}C$, $160^{\circ}C$) analysis of variance with repeated measures. The EMG activity of pectoralis major muscle was significantly different between shoulder abduction angles and between shoulder rotation positions (p<.05). The highest value of EMG activity of pectoralis major clavicle part among shoulder abduction angles was in $70^{\circ}C$ and, $90^{\circ}C$ in that order. The highest value of EMG activity of pectoralis major sternum part among shoulder abduction angles was in $130^{\circ}C$ and, $90^{\circ}C$ in that order. According to the rotation degree, shoulder ER showed the highest value and IR showed the lowest value in both muscle parts. These results suggest that shoulder abduction $70^{\circ}C$, $90^{\circ}C$, $130^{\circ}C$ will be effective during manual muscle testing (MMT) and strengthening exercise for pectoralis major muscle. It is also supposed that shoulder ER is the efficient posture for strengthening of pectoralis major muscle.

• KSBB Journal
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• v.14 no.1
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• pp.91-95
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• 1999

The objective of this work was to improve bioluminescence stability of Photobacterium phosphoreum when it stored in view of developing continuous on-line monitoring system for pullutants. Long-term experiments were made to determine the effect of immobilization and storage temperature on the maintenance and stability of bioluminescence from luminescent bacteria. The immobilized cells of P. phosphoreum were compared with free cells in terms of maintenance of bioluminescence at room temperature. The bioluminescence of cells immobilized showed higher bioluminescence intensity that free and strontium bioluminescence stability was investigated with free and immobilized cells stored at $20^{\circ}C,\; 4^{\circ}C,\; -20^{\circ}C\;and\;-70^{\circ}C$for 20 days. Both free and immobilized cells stored at $4^{\circ}C$ emitted a stable bioluminescence while the bioluminescence markedly decreased with those stored at $20^{\circ}C,\;-20^{\circ}C\;and\; -70^{\circ}C$.

• The Journal of Natural Sciences
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• v.14 no.1
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• pp.139-149
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• 2004

It was aimed to find out an efficient method of drying hot pepper, of which powder is used as one of the main ingredients in Korean dish preparation including Kimchi. Before sun or heat drying fruits harvested were horizontally cut into three pieces punched with an auger of 4 mm in diameter, or used as the whole fruit. Various drying methods were used including sun drying, drying under a plastic house, sun drying after heat drying for 5 hours at $70^{\circ}C$, $80^{\circ}C$, or $90^{\circ}C$, heat drying only at $50^{\circ}C$, $60^{\circ}C$, or $70^{\circ}C$, and changing temperature of $70^{\circ}C$, $5hours+60^{\circ}C$ until coplete dry, $80^{\circ}C+60^{\circ}C$, or $90^{\circ}C+60^{\circ}C$. The results are summarized as follows. With sun drying of the whole fruit, in required about 10days to reach the satisfactory level of dryness as indicated by its moisture content of below 10% in general. In case of heat drying combined with sun drying, it required 5 to 7 days. For cut fruits, it required 1 or 2 days less than for whole or pinholed fruits until drying. Under heat of alternation temperature drying, cut fruits also dried 15-20 hours earlier compared with whole of pinholed fruits. There were not significant differences for the recovery rate of the fruit with various drying methods. The occurrence of white fruits, which are caused by one of physiological disorders during drying, and rotten fruits was reduced with the cut fruit drying. The damages were very high with the PE house drying, but very little with the heat drying.

• Korean Journal of Food Science and Technology
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• v.21 no.6
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• pp.815-819
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• 1989

Rheological properties of chestnut starch suspensions (3 and 4%, db) and gelatinized starch (4%, db) were investigated with a capillary and rotational viscometer, respectively. Starch suspensions had no yield stress and showed dilatant flow behavior in the temperature ranges of $30-65^{\circ}C$. However, starch suspension showed pseudoplastic flow behavior at $70^{\circ}C\; and\;above\; 65^{\circ}C$ for 3 and 4% concentration, respectively Flow activation energy below $50^{\circ}C$ was 0.56 kcal/mole but increased to 51.9-80.8 kcal/mole at $60-70^{\circ}C$. The behavior of gelatinized starch (4%) was pseudoplastic regardless of heating temperature $(65-80^{\circ}C)$ and time (15-60 min). The apparent viscosity of the starch remained constant after heating at $80^{\circ}C$ for 45 min. The swelling power and log apparent viscosity showed similar pattern. The activation energy of the apparent viscosity of the geletinized starch at $70-80^{\circ}C$ was 13.09kcal/mole. The apparent viscosity of thermal-gelatinized $(90^{\circ}C)$ starch was lower than that of 15 psi-gelatinized starch.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.1
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• pp.20-28
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• 2012

The monofilament with 0.304mm of diameter was produced using a polybutylene succinate (PBS) resin, and a gill net was made by it. We investigated the impact of heat setting temperature on the mechanical properties, knot state and height of gill net. Heat treatment was carried out using the high pressure steam machine for 20 minutes at temperature of $55^{\circ}C$, $60^{\circ}C$, $70^{\circ}C$ and $75^{\circ}C$. Before heat treatment, the strength and elongation of PBS monofilament were estimated to be $48.1kg/mm^2$, 23.8% at unknot, $37.6kg/mm^2$, 18.8% at single knot, $26.6kg/mm^2$, 22.9% at double knot in dry condition, respectively. The strength and elongation of PBS monofilament with double knot were decreased as heat setting temperature increased, and the decreasing rate of strength was showed to be higher than that of elongation. It was not found any differences in strength and elongation of PBS monofilament yarn with double knot at the $65^{\circ}C$ and $70^{\circ}C$ of heat setting temperature by 5% significance of T-test, but there was a significant difference at the $70^{\circ}C$ and $75^{\circ}C$ of heat setting temperature. The net's height and length from leg to leg appeared no differences at the $70^{\circ}C$ and $75^{\circ}C$ of heat setting temperature. In results, it was investigated that the PBS monofilament gill net with the maximized physical properties could be manufactured at $70^{\circ}C$ of heat setting temperature using a high pressure steam machine for 20 minutes.