• Journal of the Korean Wood Science and Technology
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• v.32 no.4
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• pp.8-17
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• 2004

Antifungal, antibacterial, and antioxidative activities of ethanol extracts from 65 families 263 species were investigated to select tree species for the utilization of natural fungicide or preservative resources. The antifungal activities of extracts from wood, leaf and bark were measured as hyphal growth inhibition rate using four plant pathogenic and five wood rotting fungi. High inhibitory effect on the fungi growth was found in five species of Pinaceae (Pinus koraiensis, P. rigida, P. densiflora, P. banksiana. Cedrus deodara), three species of Cupressaceae (Juniperus rigida, J. chinensis, Chamaecyparis obtusa) and three species of Leguminosae (Albizzia julibrisssin, Sophora japonica, Maackia amurensis), respectively. Antibacterial activities of ethanol extracts were determined by means of disc-agar plate diffusion method using three gram-positive and five gram-negative bacteria. The ethanol extracts, which showed prominent effect on the suppression of bacteria growth, were six species of Betulaceae (Carpinus tschonoskii, C. coreana, C. laxiflora, Alnus hirsuta, A. firma, Betula schmidtii), five species of Fagaceae (Castanopsis cuspidata var. sieboldii, Quercus serrata, Q. mongolica, Q aliena, C crenata), four species of Euphorbiaceae (Aleurites fordii, Sapium sebiferum, S japonicum Mallotus japonicus) and three species of Elaeagnaceae (Elaeagnus umbellata, Elaeagnus glanbra, Elaeagnus macrophylla). According to these results, the extracts from Zelkova serrata, Pinus densiflora, Maackia amurensis, Chamaecyparis obtusa and Juniperus chinensis could be available for natural fungicide or food preservatives, because ethanol extracts from these species indicated excellent antifungal and antibacterial activities. In order to test antioxidative activities of ethanol extracts, free radical scavenging method was adopted with 1,1-diphenyl-2-picrylhydrohydrazyl (DPPH). Free radical scavenging activity was proved very high in the extracts of eight species of Rosaceae (Eriobotrya japonica, Prunus takesimensis, P yedoensis, P padus, P armeniaca var. ansu, Chaenomeles sinensis, Stephanandra incisa, Rosa multiflora) and five species of Ericaceae (Rhododenron mucronulatum, R. scblippenbacbii, R. yedoense var. poukhanense, Vaccinium bracteatum, V oldbami), resvectively. It turned out from this study that only six species among 48 species of Rosaceae showed less than 80% free radical scavenging activity. As a consequences, it could be deduced that the components effective on antioxidative activity commonly exist in Rosaceae plant family.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.41-41
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• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1073-1079
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• 2010

Forty-eight ((Duroc${\times}$Yorkshire)${\times}$Landrace) pigs with an average initial body weight (BW) of $48.47{\pm}1.13\;kg$ were used in a 12-week growth trial to investigate the influence of Anion (silicate) supplementation on growth performance, nutrient digestibility, meat quality and fecal noxious gas content in growing-finishing pigs. Pigs were allotted into three dietary treatments in a randomized complete block design according to sex and initial BW. Each dietary treatment consisted of four replications with four pigs per pen. Dietary treatments included: i) CON (basal diet), ii) HCI (basal diet+3 g/kg Anion), iii) HCII (basal diet+6 g/kg Anion). No significant difference (p>0.05) was detected for average daily gain (ADG), average daily feed intake (ADFI) and gain/feed ratio (G/F) throughout the experiment, although dietary supplementation of Anion numerically increased these characteristics compared with CON. The dietary HCI group significantly (p<0.05) increased the coefficient of total tract apparent digestibility (CTTAD) of dry matter (DM), nitrogen (N) and energy compared with the CON group (p<0.05). No significant difference was observed in meat quality except that meat firmness was linearly (p<0.05) increased by the Anion supplementation, while an increased tendency in meat color and a decreased tendency in 2-thiobarbituric acid reactive substances (TBARS) was also observed (p<0.10). Anion supplementation linearly (p<0.05) decreased the fecal $NH_3$ compared with the CON group. However, dietary Anion supplementation at 3 g/kg decreased the $H_2S$ concentration compared with CON, while no significant difference was detected in the HCII group, although the $H_2S$ emission was numerically decreased compared with CON. In conclusion, supplementation of the diet with 3 g/kg Anion was found to exert a beneficial effect on nutrient digestibility and meat quality of growing-finishing pigs, and concomitantly decreased the noxious gas emission without negative effect on growth performance.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.1
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• pp.86-94
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• 2015

This experiment was conducted to evaluate the chemical composition and amino acid (AA) digestibility of sunflower seed meal (SFSM) and to use this data to develop prediction equations for estimating AA digestibility for growing pigs. Ten SFSM were collected from five provinces in China. Twelve barrows ($38.8{\pm}4.6kg$), fitted with ileal T-cannula were allotted into two $6{\times}6$ Latin square designs. Each of six experimental periods comprised a 5-d adaption period followed by a 2-d collection of ileal digesta. The ten test diets contained 50% SFSM as the sole source of AA. Another nitrogen-free diet was used to measure the basal endogenous losses of crude protein (CP) and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. There was considerable variation (CV>10%) among the ten SFSM in chemical composition (dry matter [DM]). The concentration of CP and ether extract (EE) ranged from 29.33% to 39.09% and 0.88% to 11.33%, respectively. Crude fibre (CF), neutral detergent fibre and acid detergent fibre ranged from 21.46% to 36.42%, 38.15% to 55.40%, and 24.59% to 37.34%, respectively. There was variation among the ten SFSM in apparent ileal digestibility (AID) and standardized ileal digestibility (SID) for lysine and threonine, which ranged from 63.16 to 79.21 and 55.19% to 72.04% for AID and 67.03% to 82.07% and 61.97% to 77.01% for SID, respectively. The variation in CP and methionine ranged from 60.13% to 74.72% and 74.79% to 88.60% for AID and 66.70% to 79.31% and 77.16% to 90.27% for SID, respectively. Methionine was a good indicator to predict AA digestibility. These results indicate that conventional chemical composition of SFSM was variable (CV>10%) among the ten SFSM (DM). The results of AID, SID and prediction equations could be used to evaluate the digestibility of SFSM in growing pigs.

• Fisheries and Aquatic Sciences
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• v.22 no.11
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• pp.25.1-25.9
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• 2019

Background: Due to the continuous demand for fish coupled with decline in capture fisheries, there is the need to increase aquaculture production to meet the demand. Aquaculture is faced with high cost of feeding since fish oil and fish meal are expensive. In view of this, there are calls to explore alternatives that are cheap and reliable. Objectives: This study on Oreochromis niloticus was conducted to evaluate the effects of replacing fish oil (FO) with palm oil (PO) at 0%, 25%, 50%, 75%, and 100% on muscle fatty acid and proximate composition as well as growthrelated enzyme activities and mRNA expression. Methods: Oreochromis niloticus were fed five experimental diets (33% crude protein and 10% crude lipid) for 8 weeks. Feed had variation in fish oil and palm oil contents. After the 8 weeks feeding trial, five fish were sampled from each tank (15 from each treatment) and euthanized using an excess dose of tricaine methane sulfonate (MS-222 at 200 mg/L). Fatty acid and enzyme activities were analyzed using standard protocols. Also, RT-qPCR was used to quantify the expression levels of selected growth-related genes. Results: Fish fed 25% PO recorded the least muscle protein content and was significantly lower than the group fed 100% PO. Paired box protein 7 (Pax-7) enzyme activity was significantly higher in the group fed 50% PO compared to the groups fed 25% PO and 100% PO, while caplain-3 (Capn-3) was significantly lower in the group fed 0% PO compared to all other groups. There was a significant difference among treatments with respect to mRNA expression of Pax-7 and Capn-3. Group fed 25% PO had significantly lower mRNA expression of Pax-7, while the group fed 75% PO recorded significantly higher mRNA expression of Capn-3 compared to groups fed 0% PO, 25% PO, and 100% PO. Pearson's correlation analysis revealed that Igf-I and Igf-II mRNA expression have significant correlation with n-3 polyunsaturated fatty acids content in muscle. Conclusion: The results suggest muscle protein content could be modified if FO is replaced with PO. Also, mRNA expression of Pax-7 and Capn-3 is affected by replacing FO with PO.

• Radiation Oncology Journal
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• v.12 no.1
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• pp.17-25
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• 1994

We evaluated the effect of nicotinamide on cellular $O_{2}$ consumption and metabolic status i.e., adenylate phosphates and $NAD^{+}$in-vitro, and changes in blood flow in-vivo, to determine whether changes in cellular metabolism or increased oxygen availability, was responsible for increased tumor oxygenation. Thirty min, pre-incubation of cells with$\∼$4mM (= 500mg/kg) nicotinamide resulted in no change in cellular $O_{2}$ consumption. Similarly neither the adenylate Phosphates nor the cellular $NAD^{+}$levels were altered in the presence of $\∼$4mM nicontinamide. In-vivo, nicotinamide (500mg/kg) increased $O_{2}$ availability as estimated by changes in relative tumor blood flow (RBC flux). The changes in RBC flux measured by the laser Doppler method, were tumor volume dependent and increased from$\∼$35$ \% $ in$\∼$ 150$mm_{3}$tumors to$\∼$~75$ \% $ in$\∼$500$mm^{3}$ tumors. In conclusion, these observations indicate a reduction in local tissue $O_{2}$ consumption is not a mechanism of improved tumor oxygenation by nicotinamide in FSa II murine tumor model. The primary mechanism of increased $pO_{2}$ appears to be an increased local tumor blood flow.

• Economic and Environmental Geology
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• v.27 no.4
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• pp.375-385
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• 1994

The studied area is composed of Precambrian gneiss complex, middle Jurassic biotite granite, late Cretaceour sediments, volcanics and pink feldspar granite. Characteristic minerals of the biotite granite is plagioclase and hornblende whereas the pink feldspar granite is pink feldspar (perthite) and quartz. Plagioclase compositions of the biotite granite and the pink feldspar granite are oligoclase to calcic andesine ($An_{18-44}$) and sodic albite ($An_{0.5-5.0}$), respectively. In the variation diagrams of the Harker and normative Q-Or-Pl diagram, the biotite granite belongs to the category from granodiorite to granite, the pink feldspar granite from nomal to late granite. The values of D.I. L.I. and alkalinity of the pink feldspar granite are higher than those of the biotite granite. While CaO is enriched in the biotite granite, $K_2O$ is enriched in the pink feldspar granite. The ratio of $K_2O/Na_2O$ which indicates the relative ratio of alkali is 1.06 in the pink feldspar granite, and 0.86 in the biotite granite. In A-M-F and N-C-K diagrams both these granites are plotted in peraluminus granite ($Al_2O_3$>$Na_2O+K_2O+CaO$) region, assigned to calc alkaline series and alkaline series respectively. Put into the form of A-C-F diagram, the biotite granite falls under I-type, and the pink feldspar granite S-type. On the base of whole rock ratios of $Fe^{+3}/Fe^{+2}+Fe^{+3}$ and $^{87}Sr/^{86}Sr$ for the granites in studied area, the biotite granite indicates ilmenite series (0.26) and S-type and/or contaminated I-type ($0.72020{\pm}0.00050$), the pink feldspar granite magnetite series (0.44) and I-type ($0.70826{\pm}0.00020$).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.18 no.1
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• pp.64-80
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• 1992

Considerable interest has been generated in age-related non-enzymatic glycosylation and crosslinking of collagen in view of its extracellular nature, and its long biological half-life. The effects of UVA, which penetrates deep in dermis, and reactive oxygen species (ROS) on age- related changes of dermal collagen were studied. The amount of nonenzymatic glycosylation, fragmentation, and crosslinking of collagen were monitored from the mixtures of Type I collagen from calf skin and glucose, irradiated by UVA, with or without scavengers of ROS. At both high and low glucose dosages, non-enzymatic glycosylation was not affected by UVA irradiation. At high glucose dosage, however, glycosylation was reduced by the scavengers of superoxide radical and singlet oxygen, bolt not by hydroxyl radical scavengers. Fragmentation was increased by UVA and decreased by all ROS scavengers. Crosslinking was also enhanced by UVA, and effectively blocked crosslinking. Superoxide radical and singlet oxygen, which were produced by autoxidation of glucose independently to UVA, may encounter the initial phase of glycation. ROS generated from Amadory compounds by UVA enhanced fragmentation and crosslinking Hydroxyl radical was thought to be a major ROS affecting crosslinking. These results suggest that UVA and ROS are able to enhance age-related structural changes of collagen, as affecting many other tissue and cellular components.

• Applied Chemistry for Engineering
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• v.16 no.2
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• pp.231-237
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• 2005

The sizes and structure of micelles and vesicles formed in ammonium dodecyl sulfate (ADS)/octadecyl trimethyl ammonium chloride (OTAC) mixed aqueous solutions were analyzed by small-angle neutron scattering. In micellar regions of pure ADS and OTAC aqueous solution, the spherical micelles were formed at given concentrations and their sizes were 40 and $61{\AA}$, respectively. The structure transition of pure micelles occurred above 300 mM due to the constancy of the intermicellar distance above 250 mM. The coexisting region of mixed micelles and vesicles in phase diagram of mixed system was also assured. It was investigated that vesicle formed spontaneously took a bilayer structure. The lamellar thickness of vesicles decreased with increasing concentration of vesicle samples. However, the size could not be determined for mixed micelle and vesicle above 100 nm due to limitation of low q ranges. Finally, The 9 mM solution of ADS mole fraction 0.9 (${\alpha}$=0.9) showed bilayer structure compared to phase diagram classified into mixed micelle.

• Korean Journal of Agricultural Science
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• v.3 no.1
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• pp.105-119
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• 1976

Sucrose, glucose, starches hydrolyzates and raw starchy materials hydrolyzates were caramelized using various catalysis and the caramel products were analysed, in order to carry out the basic research for the replacement of caramel coloring. The results obtained were summarized as follows. 1. The caramel which was manufactured by sucrose syrup being pH 3.5 adjusted by sulfuric acid showed strong color intensity and hue as well as good stability in the solutions of table salt, tannin and alcohol. 2. The product caramelized from sucrose syrup being pH 9.5 adjusted by sodium carbonate showed very strong color intensity and black color component, and was quite stable in alcohol solution but not in table salt and tannin solutions. 3. The caramel products made from sucrose syrup using ammonium salts of strong acid like $NH_4Cl$ and $(NH_4)_2SO_4$ as catalyst showed strong color intensity and black color component but hazy apparence in solution of table salt, tannin and alcohol. 4. The product caramelized from glucose syrup being pH 9.5 adjusted by sodium carbonate indicated strong color intensity but weak red color component and was transparent in solution of table salt and alcohol but hazy in tannin solution. 5. In glucose caramel using $NH_4Cl$, $(NH_4)_2SO_4$, $(NH_4)_2CO_3$ and $(NH_4)_2SO_3$ as catalyst, $NH_4Cl$ plot was very weak in color intensity and insufficient in red color component but stable in solution of table salt, tannin and alcohol. In the case of $(NH_4)_2CO_3$, $(NH_4)_2SO_4$ and $(NH_4)_2SO_3$ plots, all products were strong in color intensity but little insufficient in red color component. On the stability in solutions, $(NH_4)_2SO_3$ plot was stable in two solutions expect tannin solution, $(NH_4)_2CO_3$ plot was only stable in alcohol solution and $(NH_4)_2SO_3$ plot was only stable in table salt solution. 6. When the acid hydrolyzed starch syrups without neutralization were caramelized using $(NH_4)_2SO_4$ as catalyst, the potato starch hydrolyzate caramel showed higher in color intensity being similar to its of glucose caramel than sweet potato starch hydrolyzate caramel and corn starch hydrolyzate caramel. 7. Dried sweet potato powder, dried acorns powders, the acorns (from Q. serrata THUNB and Q. acutissima CARR.) powders extracted with water for 7 days and with 50% alcohol solution for 24 hrs were hydrolyzed by sulfuric acid in autoclave at $3.5kg/cm^2$ as pressure for 60 mins, and were caramelized using $(NH_4)_2SO_4$ as catalyst. It was supposed that all of those products were poor quality on color and stability in solutions at the viewpoint of food coloring matter.