• The Korean Journal of Mycology
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• v.14 no.2
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• pp.121-130
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• 1986

Factors affecting the productivity of cellulolytic enzymes and the mycelial growth of Ganoderma lucidum CAFM 9065 were examined in synthetic media. Among the carbon sources tested, Na-CMC was the best for the production of avicelase CMC ase, and cellobiose for ${\beta}-glucosidase$. Soluble starch and cellobiose were the best for the mycelial growth. The optimum concentration of Na-CMC for the production of the enzymes was 1.0 %, and mycelial growth increased remarkably with the higher concentration of Na-CMC. Glucose inhibited the production of the enzymes, but stimulated the mycelial growth. Among the nitrogen sources used, peptone was the most effective for the production of the enzymes, and the appropriate concentration of peptone was 0.2%. The mycelial growth was stimulated with the increase of the concentration of peptone up to 0.5%. The optimum concentration of $KH_2PO_4$ for the production of the enzymes and mycelial growth was 0.3 and 0.2%, respectively. The optimum concentration of $MgSO_4{\cdot}7H_2O$ for the production of the enzymes and mycelial growth was 0.02%. The production of the enzymes was facilitated by folic acid at a low concentration (0.03 mg/l), and mycelial growth by inositol. The optimum temperature for the production of the enzymes and mycelial growth was $30^{\circ}C$. The optimum pH for the production of avicelase and ${\beta}-glucosidase$ was 5.0 equally and CMCase 5.5. The activities of avicelase and CMCase were the highest at 8 and 10 days of culture, respectively and that of ${\beta}-glucosidase$ at 16 day culture. The growth of mycelium was the highest at 12 days of culture at pH 5.0.