• 제목/요약/키워드: ${\beta}$-Strength

검색결과 532건 처리시간 0.026초

Analysis of Promoter Strength of Autographa californica Nuclear Polyhedrosis Virus IE1 Gene by Using Rreconmbinant Baculovirus

  • Cho, Eun-Sook;Park, Hae-Jin;Jin, Byung-Rae;Sohn, Hung-Dae;Kang, Seok-Woo;Yun, Eun-Young;Kim, Keun-Young;Je, Yeon-Ho;Kang, Seok-Kwon
    • 한국잠사곤충학회지
    • /
    • 제41권2호
    • /
    • pp.102-107
    • /
    • 1999
  • To analysis a promoter strength of Atographa californica nucler polyhedrosis virus (AcNPV) IE1 gene, an immediate viral gene, ${\beta}$-glactosidase gene as a reporter gene was introduced under the control of the IE1 promoter. The restriction fragment containing IE1 promoter and ${\beta}$-galctosidase gene from pAcIE1-gal were inserter into pBacPAK9 to yield transfer vector pAcNPV-IE1-gal. The pAcNPV-IE1-gal was cotransfected with AcNPV genomic DNA BacPAK6 into Sf9 cells to produce recombinant baculovirus AcNPV-IE1-gal. In addition, recombinant bacvulovirus AcNPV-gal, which express ${\beta}$-galac-tosidase under the control of the polyhedrin promoter, was constrer, was constructed to compared with AcNPV-IE1-gal. The recombinant viruses were respectively infected into Sf9 cells and characterized by the virus titer and expression of ${\beta}$-galactoxidase in Sf9 cells. The promoter strength of IE1 and polyhedrin promoters was determined by the amount of ${\beta}$-galactosidase secreted into medium by viral infection. The titer of AcNPV-IE1-Gal determined by plaque assays in Sf9 cells was similar to that of AcNPV-gal. However, expression level of ${\beta}$-galactosidase by AcNPV-IE1-gal was significantly lower than that by AcNPV-gal. In conclusion, promoter strength of IE1 was approximately 25-fold lower than that of polyhedrin.

  • PDF

질화규소의 기계적 성질 및 접촉 손상: I. ${\alpha}/{\beta}$ 상분율의 영향 (A Study on the Mechanical Properties and Contact Damage of Silicon Nitrides : 1. Effect of ${\alpha}/{\beta}$ Phase Fraction)

  • 이승건
    • 한국분말재료학회지
    • /
    • 제5권1호
    • /
    • pp.15-21
    • /
    • 1998
  • The effect of $\alpha$/$\beta$ phase on the mechanical properties and contact damage of silicon nitrides $Si_3N_4$) was investigated. Silicon nitride materials were prepared from two starting powders, at selective increasing hot-pressing temperatures to coarsen the microstructures: (i) from relatively coarse $\alpha$-phase powder, essentially equiaxed $\alpha$-$Si_3N_4$ grains, with limited, slow transformation to $\beta$-$Si_3N_4$ grain; (ii) from relatively fine $\alpha$-phase powder, a more rapid transformation to $\beta$-$Si_3N_4$, with attendant grain elongation. The resulting micro-structure thereby provided a spectrum of $\alpha$/$\beta$ phase ratios, grain sizes, and grain shapes. Fracture strength, hardness, and toughness were measured, and contact damage and strength degradation after indentation were investigated by Hertzian indentation using spherical indenter. A brittle to ductile transition in $Si_3N_4$ depended on $\alpha$/$\beta$ phase ratio as well as grain size. Silicon nitride with elongated $\beta$ grains showed a superior, contact damage resistance.

  • PDF

Influence of Various Cyclodextrins on the Stability of Hydrocortisone 17-Butyrate in Aqueous Solution

  • Chun, In-Koo;Kim, Bo-Young
    • Archives of Pharmacal Research
    • /
    • 제15권2호
    • /
    • pp.176-183
    • /
    • 1992
  • The stabilizing effects of $\alpha$-$\beta$-$\gamma$- and dimethyl-$\beta$-cyclodextrins $(\alpha$-, $\beta$-, $\gamma$- and DM-$\beta-$-CyDs) on the degradation of hydrocortisone 17-butyrate (HC-17B) in aqueous solution was investigated. Hc-17B underwent a facile hydroxide ion-catalyzed rearrangement to the less active 21-butyrate ester by the apparent first-order kinetics, and maximum stability of HC-17B was obtained at around pH 4.0. The stability of HC-17B was increased by inclusion complexation with $\alpha$-, $\gamma$- and DM-$\beta$-CyD in the pH range of 2.0-8.0 examined, whereas $\beta$-CyD accelerated the degradation of HC-17B at the pH higher than 5.0. The effects of ionic strength, solvent, temperature and CyD concentration were also investigated. Stability constants and apparent degradation rate constants of HC-17B-$\gamma$-CyD and HC-17B-DM-$\beta$-CyD complexes were determined kinetically on the basis of 1:1 complexation. The results suggested that the inclusion complexation with $\gamma$-CyD or DM-$\beta$-CyD was most useful means to enhance the stability of the steroid.

  • PDF

TTCP-DCPA-β-TCP-PHA계 골 시멘트 (Bone Cements in TTCP, DCPA, β-TCP and PHA System)

  • 김형준;최성철;석준원
    • 한국세라믹학회지
    • /
    • 제39권1호
    • /
    • pp.57-67
    • /
    • 2002
  • 본 연구에서는 Tetracalcium Phosphate(TTCP), Dicalcium Phosphate Anhydrate(DCPA)계 골 시멘트에 $\beta$-tricalcium Phosphate (TCP)와 Precipitated Hydroxy Apatite(PHA)를 첨가하였을 때, 골시멘트의 초기 응결시간과 강도에 미치는 영향 및 in vitro test후의 표면 미세구조의 변화를 관찰하고자 하였다. 골 시멘트에 사용된 TTCP와 $\beta$-TCP는 약 3-5$mu extrm{m}$으로 합성후 분쇄하였으며, DCPA는 0.9$\mu\textrm{m}$, 그리고 PHA는 4$\mu\textrm{m}$의 평균 입경을 가졌다. 각각의 조성으로 배합된 시멘트는 Vicat건에 의한 초기응결시간 측정과 압축강도 시험을 행하였고, 의사체액내에 침적 후 침전 생성물을 x-선 회절 분석과 주사전자현미경을 이용하여 분석, 관찰 하였다. 초기 응결시간은 $\beta$-TCP나 PHA의 존재 유무와 함량의 증가에 따라 크게 좌우되지 않았으나, 분말 : 액상의 비에 영향을 받았으며, 특히 PHA가 함유되는 경우 PHA의 비표면적으로 인하여 응결에 요구되는 액상의 양은 PHA가 없는 경우에 비하여 2배 이상 되었다. $\beta$-TCP PHA의 첨가로 인해 압축강도는 낮아졌고, 이는 수화 생성물인 HAP의 생성 정도가 낮았기 때문이었다. 이는 x-선 회절 분석과 주사전자 현미경 관찰을 통하여 확인할 수 있었다. 본 연구로부터 TTCP-DCPA계 골 시멘트에 $\beta$-TCP나 PHA의 첨가는 기계적 물성과 생체 반응성 향상에 효과적이지 못하다는 것을 확인 할 수 있었다.

거대기공 다공질 탄화규소 세라믹스의 꺾임강도 (Flexural Strength of Macroporous Silicon Carbide Ceramics)

  • 임광영;김영욱;송인혁;배지수
    • 한국세라믹학회지
    • /
    • 제48권5호
    • /
    • pp.360-367
    • /
    • 2011
  • Macroporous silicon carbide (SiC) ceramics were fabricated by powder processing and polymer processing using carbon-filled polysiloxane as a precursor. The effects of the starting SiC polytype, template type, and template content on porosity and flexural strength of macroporous SiC ceramics were investigated. The ${\beta}$-SiC powder as a starting material or a filler led to higher porosity than ${\alpha}$-SiC powder, owing to the impingement of growing ${\alpha}$-SiC grains, which were transformed from ${\beta}$-SiC during sintering. Typical flexural strength of powder-processed macroporous SiC ceramics fabricated from ${\alpha}$-SiC starting powder and polymer microbeads was 127 MPa at 29% porosity. In contrast, that of polymer-processed macroporous SiC ceramics fabricated from carbon-filled polysiloxane, ${\beta}$-SiC fillers, and hollow microspheres was 116MPa at 29% porosity. The combination of ${\alpha}$-SiC starting powder and a fairly large amount (10 wt%) of $Al_2O_3-Y_2O_3$ additives led to macroporous SiC ceramics with excellent flexural strength.

DV-Xα 분자 궤도법을 이용한 고강도 타이타늄 합금 설계 (A Study on the Design of High-Stength Titanium Alloys Using DV-Xα Molecular Orbital Method)

  • 백민숙;윤동주;원대희;김병일
    • 대한금속재료학회지
    • /
    • 제49권9호
    • /
    • pp.739-745
    • /
    • 2011
  • Beta-type alloys are the most versatile class of titanium alloys. They offer the highest strength to weight ratios and very attractive combinations of strength, toughness, and fatigue resistance inlarge cross sections [1]. The present study was made to obtain useful information for the design of ${\beta}$-type titanium alloys with high-strength properties by using the $DV-X{\alpha}$ method. Employing two calculated parameters, the bond order (Bo) and the d-orbital energy level (Md) of alloying elements in ${\beta}$-type titanium alloy was introduced and used for prediction of mechanical properties. Thus, high-strength titanium alloys were designed by calculating the Md and Bo values of the previous and present titanium alloys.

Hot Pressing of the Silicon Nitride Based Ceramics and Their Mechanical Behavior

  • Park, D.S.;Lee, S.Y.;Kim, H.D.;Park, W.S.;D.S. Lim;B.D. Han
    • The Korean Journal of Ceramics
    • /
    • 제1권1호
    • /
    • pp.45-54
    • /
    • 1995
  • Four kinds of silicon nitride based ceramic materials have been hot pressed. Effect of the sintering additives on the phase transformation, microstructural development and mechanical properties was investigated. While sintering under the same condition a big difference among the microstructures of the specimens, they appeared alike if sintered to have a similiar $\alpha$-$\beta$ phase ratio. The specimen of the stoichiometric $\alpha$-$\beta$ sialon composition showed very limited amount of the intergranular glassy phase and a significant degree of the residual stress. It exhibited almost no strength degradation up to $1300^{\circ}C$, and the strength of the specimen degraded more as its composition deviated from the stoichiometry.

  • PDF

플라이애시 및 고로슬래그 미분말을 사용한 콘크리트의 강도 발현 예측 모델식 연구 (A Study on Estimation Model of Strength Development of Concrete Using Fly Ash and Ground Granulated Blast-Furnace Slag)

  • 최연왕;박만석;정재권;최병걸;김경환
    • 한국구조물진단유지관리공학회 논문집
    • /
    • 제17권5호
    • /
    • pp.87-93
    • /
    • 2013
  • 최근 콘크리트 산업은 $CO_2$ 가스 배출 저감을 목적으로 플라이애시 및 고로슬래그 미분말 등의 혼화재 사용량이 증가되고 있다. 그러나 국내의 경우 구조설계기준에서 사용되고 있는 강도 발현 예측 모델은 시멘트 종류 및 양생방법에 따른 상수값 ${\beta}_{sc}$를 포틀랜드시멘트에 관해서만 규정함에 따라 플라이애시 및 고로슬래그 미분말을 혼합 사용한 콘크리트의 시간에 따른 강도 발현 특성을 반영하지 못하는 실정이다. 따라서 본 논문에서는 플라이애시 및 고로스래그 미분말을 혼합한 보통강도 및 고강도 영역의 콘크리트의 시간에 따른 강도를 평가하였으며, 혼화재 종류 및 혼합률에 따른 콘크리트 강도발현 상수 ${\beta}_{sc}$ 값을 제안하였다.

충남 청양군 운곡면 일원에 분포하는 편마암의 강도이방성 특성 - 점재하강도지수와 일축압축강도의 관계를 중심으로 - (The Anisotropic Mechanical Characteristics of the Metamorphic Rocks Distributed in the Samkwang-Mine Area, Cheongyang, Chungnam)

  • 배대석;송무영;김경수
    • 지질공학
    • /
    • 제1권1호
    • /
    • pp.54-67
    • /
    • 1991
  • 일반적으로 모든 암석은 외경상의 특징과 무관하게 어느 정도의 구조적 및 역학적 이방성을 갖고 있다. 점재하강도지수(point-load strength index : I$_s$)를 이용한 일축압축강도(uniaxial compressive strength, UCS : ${\sigma}_c$)의 추정시, 이들 두 값은 암석이 갖는 취약면구조의 방향에 따라서 그 변화 양상에 차이가 있다. 일반적으로 재하의 방향과 취약면이 이루는 각이 직교 및 평행일 때 ${\sigma}_c$는 최대치를 나타내며, 약 30$^{\circ}$~60$^{\circ}$ 범위에서 값의 변화가 심하다. 이에 대하여, I$_s$의 경우 취약면이 방향에 따라 강도이방성 현상은 ${\sigma}_c$의 경우와 구별된다. 수직 방향으로 채취한 시추 코아에서 수평 방향으로 측정한 직경점재하강도지수(diametral point-load strength index : I$_{sd}$)는 코아의 축방향과 취약면에대한 법선과이루는 각($\beta$)이 0$^{\circ}$에서 90$^{\circ}$로 증가함에 따라 비례하여 증가하는데 반해서, 축점재하강도지수(axial point-load strength index : I$_{sa}$)는 감소하는 경향을 보인다. 청양군 운곡면 일원에 분포하는 호상편마암에서 ${\sigma}_c$와 I$_s$의 관계는 $\beta$가 0$^{\circ}$~40$^{\circ}$ 구간에서는 ${\sigma}_c$와 I$_{sa}$, 40$^{\circ}$~90$^{\circ}$ 구간에서는 ${\sigma}_c$와 I$_{sd}$가 각각 양호한 상관관계를 보여준다. 또한 상관비(K=${\sigma}_c$/I$_s$)는 약 13정도로서 일반적으로 적용되는 비, 24와 상당한 차이가 있다. 이러한 현상은 호상편마암의 구조적 및 역학적 이방성 특성으로 인한 결과라고 판단된다. 한편 맥암류에서 K가 약 23정도로서 일반적인 비 24에 상당히 접근한다. 따라서, 이방성 구조가 뚜렷한 암석에서 상관비 24는 항상 적용할 수는 없으며 일축압축강도시험과 병행 실시하여 적용하는 것이 바람직하다.

  • PDF

Aluminoborate계 유리질을 사용한 $\textrm{ZrO}_2$/Na $\beta$"-알루미나 복합재와 $\alpha$-알루미나간의 접합 (Joining of $\textrm{ZrO}_2$/Na $\beta$"-Alumina to $\alpha$-Alumina using Aluminoborate Glass Sealant)

  • 박상면;최기용;박정용;김경흠
    • 한국재료학회지
    • /
    • 제9권1호
    • /
    • pp.35-41
    • /
    • 1999
  • In this study we investigated the effects of process variables on the bond strength, and its dependency upon the interfacial chemistry when the joined $ZrO_2$ toughened $Na\beta$"-alumina to $\alpha$-alumina using B$_2$$O_3$-$SiO_2$-Al$_2$$O_3$-CaO glass sealant. We observed that bond strength is mainly determined by the strength of the glass, which, in turn, depends on the glass composition established after joining reaction. Joining at $950^{\circ}C$ for 15min yielded the highest average bond strength of 66MPa. Different types of interfacial reaction seem to occur at each interface. After joining at $950^{\circ}C$ for 15min we found that Ca and Si diffuse much deeper(~15$\mu\textrm{m}$) into the $\beta$"-alumina composite than into the $\alpha$-alumina(<1$\mu\textrm{m}$) as a result of ion exchange reaction and more effective grain boundary diffusion. Thermal expansion coefficient of the glass was found to have changed more closely to those of the $\beta$"-alumina composite and $\alpha$-alumina, which put the glass under a slight compressive stress.ressive stress.

  • PDF