• Title/Summary/Keyword: ${\L}C$

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Effects of sodium bicarbonate as an inorganic carbon source on the growth of scenedesmus dimorphus (무기탄소원으로서의 NaHCO3가 미세조류 Scenedesmus dimorphus의 성장에 미치는 영향 평가)

  • Joo, Sung-Jin;Zhang, Shan;Choi, Kyoung Jin;Lee, SeokMin;Hwang, Sun-Jin
    • Journal of Korean Society of Water and Wastewater
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    • v.28 no.5
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    • pp.555-560
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    • 2014
  • This study investigates the effect of sodium bicarbonate ($NaHCO_3$) on growth of S.dimorphus. $NaHCO_3$ concentration was varied from 0 to 2 g-C/L. As a result, the increase in concentration of $NaHCO_3$ up to 1.5 g-C/L increased dry weight of algae. The highest specific growth rate of S. dimorphus was $0.36day^{-1}$ which was obtained at concentration of 0.5 g-C/L $NaHCO_3$. pH showed a large variation range at the concentrations lower than 0.5 g-C/L $NaHCO_3$ whereas inorganic carbon, nitrate and phosphorus removal rates were almost same at the concentrations higher than 0.5 g-C/L $NaHCO_3$ (0.75, 1, 1.25, 1.5, 2 g-C/L $NaHCO_3$). Their average inorganic carbon, nitrate and phosphorus removal rate were 70 mg-C/L/d, 11.3 mg-N/L/d, and 1.6 mg-P/L/d, respectively. Thus, $NaHCO_3$ didn't effect on inorganic carbon, nitrate and phosphorus removal rate of S. dimorphus.

Decision Level Fusion of Multifrequency Polarimetric SAR Data Using Target Decomposition based Features and a Probabilistic Ratio Model (타겟 분해 기반 특징과 확률비 모델을 이용한 다중 주파수 편광 SAR 자료의 결정 수준 융합)

  • Chi, Kwang-Hoon;Park, No-Wook
    • Korean Journal of Remote Sensing
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    • v.23 no.2
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    • pp.89-101
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    • 2007
  • This paper investigates the effects of the fusion of multifrequency (C and L bands) polarimetric SAR data in land-cover classification. NASA JPL AIRSAR C and L bands data were used to supervised classification in an agricultural area to simulate the integration of ALOS PALSAR and Radarsat-2 SAR data to be available. Several scattering features derived from target decomposition based on eigen value/vector analysis were used as input for a support vector machines classifier and then the posteriori probabilities for each frequency SAR data were integrated by applying a probabilistic ratio model as a decision level fusion methodology. From the case study results, L band data had the proper amount of penetration power and showed better classification accuracy improvement (about 22%) over C band data which did not have enough penetration. When all frequency data were fused for the classification, a significant improvement of about 10% in overall classification accuracy was achieved thanks to an increase of discrimination capability for each class, compared with the case of L band Shh data.

Overproduction of Bacterial Trypsin in Streptomyces - Optimization for Streptomyces griseus Trypsin Production by Recombinant Streptomyces (미생물을 이용한 트립신 과대 생산 연구 - Streptomyces용 숙주-벡터계를 이용한 트립신 유전자의 대량발현 최적화 -)

  • Kim, Jong-Hee;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.28-33
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    • 2008
  • The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.

A Fundamental Study on Utilization of Photosynthetic Bacteria Metabolites (광합성세균 균체대사산물의 자원화에 대한 기초적 연구)

  • Choi, Kyung-Min;Yang, Jae-Kyung;Park, Eung-Roh;Bae, Jin-Woo;Seo, Yong-Ki;Lee, Sung-Taik
    • Journal of the Korea Organic Resources Recycling Association
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    • v.5 no.1
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    • pp.63-69
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    • 1997
  • The role of L-glutamic acid, a precursor of $C_5$ ALA biosynthetic pathway, on the production of 5-aminolevulinic acid (ALA) has been described in cells of Rhodospirillum rubrum N-1. To the Lascelles basal medium the addition of both 30 mM L-glutamicacid and 20 mM levulinic acid (LA) provided to increase the extracellular ALA yield up to 40 fold (76 mg/l). By the addition of both 60 mM glycine and succinic acid, precursorsof $C_4$ ALA biosynthetic pathway, at middle log phase of cell growth ALA yield was increased 27 fold (52 mg/l) although the celt growth was inhibited to a certain extent.

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Analysis on Effects of Permeability in Contaminated Area on Extraction of Contaminants from Soil Using Vertical Drains (연직배수재에 의한 토양오염물질 추출에 지반의 투수계수가 미치는 영향분석)

  • Lee, Haeng-Woo;Chang, Pyoung-Wuck;Kang, Byung-Yoon
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.10a
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    • pp.373-381
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    • 2005
  • The permeability of contaminated soil and elapsed time are important considering factors to in-situ soil remadiation. Gabr et. al. (1996) derived the solution equation of contaminant concentration ratio as initial one (C/$C_0$) with time and spatial changes in contaminated area which embedded with vertical drains. The contaminant concentration ratio (C/$C_0$) is analyzed with time and spatial changes in three different permeability areas which are $k=l.0{\times}10^{-5,}$ $l.0{\times}l0^{-6,}$ $l.0{\times}l0^{-7}\;_{m/s}$ by using the Gabr's equation. Results from numerical analysis indicate that the ratio (C/$C_0$) decreases as the elapsed time increases in every point, however, remediation efficiency decreases as the analyzing point is far from injection well to extraction one and is deeper from top level of contaminated area. And also it decreases as the permeability of contaminated area decreases. Especially, the lower permeability of contaminated area effects directly on the soil remediation, in this research, under condition which the permeability of contaminated area is $l.0{\times}l0^{-7}\;_{m/s}$, the maximum time needed to attain 90% clean up level ($t_{90}$) is 65,690 hours(7.5 years), it takes so much time to clean the low permeability contaminated soil.

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A Study on the Application of Principle of Good Faith in L/C Base Transaction (신용장(信用狀) 거래(去來)에 있어 신의성실(信義誠實) 원칙(原則)의 적용(適用)에 관한 고찰(考察))

  • Shin, Koon-Jae;Kim, Kyung-Bae
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.22
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    • pp.173-197
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    • 2004
  • Letter of Credit between buyer and seller in International Trade Transaction is the means of payment which makes International Trade operate smoothly by guaranteeing an exporter against non-payment and an importer against non-delivery. Therefore, the parties to a sale apply UCP500 established by the International Chamber of Commerce, in accordance with principle of the freedom of contract among the parties concerned, to look to their own legal stability. However, we may recognize some cases to have been applied principle of faith and trust, one of the dominant principles of the civil law, by the Korean Supreme Court and other cases to have not been applied that principle by the Korean Supreme Court. The Court shall apply UCP500 strictly as long as the parties concerned adopt UCP500 in view of the legal stability. In other words, in case that the Court applies principle of faith and trust to the case related to L/C, this rule - principle of faith and trust - should apply to the subject matter which have not stipulated in UCP500 under certain restriction. We suggest keeping in mind points to korean companies as follows; First, the parties to a sale shall understand L/C basis transaction and principles related to L/C deeply. Second, the exporter shall prepare documents in compliance with L/C and fulfil his or her obligation according to UCP500 and L/C related to the contract. Third, as buyer or importer, when he or she receive the shipping documents with discrepancies from the notifying bank, he or she makes him or herself clear to all the parties concerned. Fourth, as bank, she shall examine all the documents according to UCP500 and L/C related to the contract, and if any document with discrepancies, the bank, by all means, shall approach applicant first, and then decide whether to pay the credit amount to beneficiary or not to.

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The Effect of Bacteriocin Produced by Lactobacillus plantarum on the Growth of Listeria monocytogenes

  • Kim Sang-Hyun;Lee Jong-Gab;Lee Myung-Suk
    • Fisheries and Aquatic Sciences
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    • v.1 no.1
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    • pp.35-41
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    • 1998
  • The inhibitory effect of Lactobacillus plantarum (Lb. plantarum) which is bacteriocin­producing strain against the growth of Listeria monocytogenes (L. monocytogenes) was examined in trypticase soy broth (TSB). TSB was inoculated with 104 cells/me L. monocytogenes and then with different numbers $(10^6\;10^4\;and\;10^2\;cells/ml)$ of Lb. plantarum. The mixed cultures were incubated at 37, 25 and $4^{\circ}C$. The most effective inhibition of was found at $37^{\circ}C$ and a less inhibition at $25^{\circ}C$. However, there was no significant change in the cell numbers of both L. monocytogenes and Lb. plantarum at $4^{\circ}C$. At same incubation temperature, the higher initial inoculum level of Lb. plantarum, the better inhibitory effect against L. monocytogenes. In addition, TSB was inoculated with L. monocytogenes at different initial inoculum levels of $10^6,\;10^4$ and $10^2$ cells/me and then supplemented with 0, 30, 60 and 100 AU/ml of bacteriocin produced by Lb. plantarum. The mixed cultures were incubated at 37, 25 and $4^{\circ}C$. L. monocytogenes of three different initial inoculum levels began to be inhibited in the presence of more than 60 AU/ml of bacteriocin at $37^{\circ}C$. In TSB containing more than 60 AU/me of bacteriocin and incubated at $25^{\circ}C$, L. monocytogenes decreased by 2 log-units during the period of 12 hrs incubation and thereafter remained steady. At $4^{\circ}C$, L. monocytogenes decreased by 1.5 log-units in the presence of 60 AU/ml bacteriocin during the period of 4 days incubation and dropped to the non-detectable level in TSB with 100 AU/ml bacteriocin.

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GPS-X Based Modeling on the Process of Gang-byeon Sewage Treatment Plant and Design of Recycle Water Treatment Process (GPS-X 기반 모델링에 의한 강변사업소 처리효율 분석 및 반류수 처리 공정 설계)

  • Shin, Choon Hwan
    • Journal of Environmental Science International
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    • v.25 no.11
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    • pp.1493-1498
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    • 2016
  • The efficiencies of Gang-Byeon sewage treatment facilities, which are based on GPS-X modelling, were analysed and used to design recycle water treatment processes. The effluent of an aeration tank contained total kjeldahl nitrogen (TKN) of 1.8 mg/L with both C-1 and C-2 conditions, confirming that most ammonia nitrogen ($NH_3{^+}-N$) was converted to nitrate nitrogen ($NO_3{^-}-N$). The concentrations of $NH_3{^+}-N$ and $NO_3{^-}-N$ were found to be 222.5 and 227.2 mg/L, respectively, with C-1 conditions and 212.2 and 80.4 mg/L with C-2 conditions. Although C-2 conditions with higher organic matter yielded a slightly higher nitrogen removal efficiency, sufficient denitrification was not observed to meet the discharge standards. For the total nitrogen (T-N) removal efficiency, the final effluent concentrations of T-N were 293.8 mg/L with biochemical oxygen demand (BOD) of 2,500 mg/L, being about 1.5 times lower than that (445.3 mg/L) with BOD of 2,000 mg/L. Therefore, an external carbon source to increase the C/N ratio was required to get sufficient denitrification. During the winter period with temperature less than $10^{\circ}C$, the denitrification efficiency was dropped rapidly even with a high TKN concentration (1,500 mg/L). This indicates that unit reactors (anoxic/aerobic tanks) for winter need to be installed to increase the hydraulic retention time. Thus, to enhance nitrification and denitrification efficiencies, flexible operations with seasons are recommended for nitrification/anoxic/denitrification tanks.

Outcomes and Use of Therapeutic Drug Monitoring in Multidrug-Resistant Tuberculosis Patients Treated in Virginia, 2009-2014

  • Heysell, Scott K.;Moore, Jane L.;Peloquin, Charles A.;Ashkin, David;Houpt, Eric R.
    • Tuberculosis and Respiratory Diseases
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    • v.78 no.2
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    • pp.78-84
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    • 2015
  • Background: Reports of therapeutic drug monitoring (TDM) for second-line medications to treat multidrug-resistant tuberculosis (MDR-TB) remain limited. Methods: A retrospective cohort from the Virginia state tuberculosis (TB) registry, 2009-2014, was analyzed for TDM usage in MDR-TB. Drug concentrations, measured at time of estimated peak ($C_{max}$), were compared to expected ranges. Results: Of 10 patients with MDR-TB, 8 (80%) had TDM for at least one drug (maximum 6 drugs). Second-line drugs tested were cycloserine in seven patients (mean $C_{2hr}$, $16.6{\pm}10.2{\mu}g/mL$; 4 [57%] below expected range); moxifloxacin in five (mean $C_{2hr}$, $3.2{\pm}1.5{\mu}g/mL$; 1 [20%] below); capreomycin in five (mean $C_{2hr}$, $21.5{\pm}14.0{\mu}g/mL$; 3 [60%] below); para-aminosalicylic acid in five (mean $C_{6hr}$, $65.0{\pm}29.1{\mu}g/mL$; all within or above); linezolid in three (mean $C_{2hr}$, $11.4{\pm}4.1{\mu}g/mL$, 1 [33%] below); amikacin in two (mean $C_{2hr}$, $35.3{\pm}3.7{\mu}g/mL$; 1 [50%] below); ethionamide in one ($C_{2hr}$, $1.49{\mu}g/mL$, within expected). Two patients died: a 38-year-old woman with human immunodeficiency virus/acquired immune deficiency syndrome and TB meningitis without TDM, and a 76-year-old man with fluoroquinolone-resistant (pre-extensively drug-resistant) pulmonary TB and low linezolid and capreomycin concentrations. Conclusion: Individual pharmacokinetic variability was common. A more standardized approach to TDM for MDR-TB may limit over-testing and maximize therapeutic gain.