• Applied Biological Chemistry
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• 제47권2호
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• pp.270-273
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• 2004

꾸지뽕나무(Cudrania tricuspidata Bureau)의 클로로포름 추출물로부터 2종의 prenylated flavonoid 항균 물질을 분리하였다. 분리할 화합물에 대하여 항균활성을 실험 한 결과 화합물 1과 2는 $10\;{\mu}g/disk$의 농도에서 Gram 양성균인 Staphylococcus aureus, Bacillus subtilis 그리고 Bacillus cereus에 대하여 활성을 보였다. 이들의 구조를 $^lH\;NMR,\;^{13}C\;NMR$ 및 2D NMR을 포함한 분광학적인 방법을 통하여 분석한 결과, 화합물 1과 2는 euchrestaflavanone B와 euchrestaflavanone C로 각각 동정 되었다.

• Bulletin of the Korean Chemical Society
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• 제7권6호
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• pp.413-421
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• 1986

Hydrocarbon solution of $Cp_2Zr(CH_3)Cl$ react rapidly with $Na_2Fe(CO)_4$ (1/2 equiv.) to yield $[Cp_2Zr(CH_3)]_2Fe(CO)_4$ and NaCl. The more soluble metal-metal bonded complex $[Cp_2ZrC_8H_{17}]_2Fe(CO)_2$ has also been prepared through the reaction of $Cp_2Zr(C_8H_{17})BF_4$ and $Na_2Fe(CO)_4 (1/2 equiv.). The complexes were characterized by IR, $^1H$ NMR, ^{13}C$ NMR, and elemental analysis. The infrared spectrum of $[Cp_2ZrR]_2Fe(CO)_4$ shows four bands, which is indicative of a cis-structure. The $^{13}C$ NMR spectrum provides evidence for the cis-structure.

• Journal of Ginseng Research
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• 제38권3호
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• pp.194-202
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• 2014

Background: Ginsenosides, the major ingredients of Panax ginseng, have been studied for many decades in Asian countries as a result of their wide range of pharmacological properties. The less polar ginsenosides, with one or two sugar residues, are not present in nature and are produced during manufacturing processes by methods such as heating, steaming, acid hydrolysis, and enzyme reactions. $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the identification of the less polar ginsenosides are often unavailable or incomplete. Methods: We isolated 21 compounds, including 10 pairs of 20(S) and 20(R) less polar ginsenosides (1-20), and an oleanane-type triterpene (21) from a processed ginseng preparation and obtained complete $^1H$-NMR and $^{13}C$-NMR spectroscopic data for the following compounds, referred to as compounds 1-21 for rapid identification: 20(S)-ginsenosides Rh2 (1), 20(R)-Rh2 (2), 20(S)-Rg3 (3), 20(R)-Rg3 (4), 6'-O-acetyl-20(S)-Rh2 [20(S)-AcetylRh2] (5), 20(R)-AcetylRh2 (6), 25-hydroxy-20(S)-Rh2 (7), 25-hydroxy-20(S)-Rh2 (8), 20(S)-Rh1 (9), 20(R)-Rh1 (10), 20(S)-Rg2 (11), 20(R)-Rg2 (12), 25-hydroxy-20(S)-Rh1 (13), 25-hydroxy-20(R)-Rh1 (14), 20(S)-AcetylRg2 (15), 20(R)-AcetylRg2 (16), Rh4 (17), Rg5 (18), Rk1 (19), 25-hydroxy-Rh4 (20), and oleanolic acid 28-O-b-D-glucopyranoside (21).

• Bulletin of the Korean Chemical Society
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• 제26권4호
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• pp.603-608
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• 2005

The $^1H\;and\;^{13}C$ chemical shifts of flavone and its five derivatives were determined completely using the basic 1D and 2D NMR experiments and molecular modeling. Of the six compounds used for our experiments, the NMR data of three compounds were published previously, but we found that the data of two compounds included wrong assignments. Therefore, we report the corrected data and the complete assignments of NMR data of the other three compounds.

• Journal of the Korean Wood Science and Technology
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• 제35권6호
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• pp.145-151
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• 2007

산겨릅나무 수피의 성분 연구를 위해 수피를 채취하여 70% 아세톤 용액으로 추출하고 농축한 후 분획깔때기로 헥산, 디클로로메탄, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성 분획에 대하여 Sephadex LH-20 칼럼크로마토그래피를 반복적으로 수행하여 5개의 페놀성 화합물을 분리하였다. 화합물의 구조는 $^1H-NMR$, $^{13}C-NMR$, 2D-NMR 및 MS 스펙트럼을 분석하여, (+)-catechin (1), (-)-epicatechin (2), (-)-epicatechin-3-O-gallate (3), gallic acid (4) 및 6''-O-galloylsalidroside (5)으로 동정하였다. (-)-epicatechin-3-O-gallate (3), gallic acid (4), 6''-O-galloylsalidroside (5)는 산겨릅나무 수피에서 처음 분리되었다.

• 생약학회지
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• 제27권3호
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• pp.207-211
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• 1996

Cumambrin A has been isolated from the dried flowers of Chrysanthemum boreale Makino. The complete $^1H$ and $^{13}C$ NMR assignment of cumambrin A was achieved from two-dimensional $^1H$-$^1H$ COSY and $^{13}C$-$^1H$ COSY spectra with the aid of homonuclear and heteronuclear double resonance experiments. The its structure has been verified by single crystal X-ray diffraction.

• 한국잠사곤충학회지
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• 제38권2호
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• pp.180-185
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• 1996

가잠 실크 피브로인의 아미노산 조성 중 가장 많은 비율을 차지하고 있는 Glycine에 동위체 라벨링([1-13C]-Gly)을 실시하여 5령 1일째부터 고치를 지을 때까지의 생합성의 상태를 NMR법을 이용하여 추적하였다. 그 결과 5령 5일을 기준으로 가잠 실크의 결정부분을 형성하는 아미노산이 형성은 되나 실크의 결정부분을 형성하는 아미노산이 형성은 되나 실크가 되었을 때 결정성의 고배향 구조를 이루지 못하고 결정성의 저배향 구조를 형성하고마는 형태로 된다고 생각되어 졌다. 이 사실은 배향시료의 고체CP-NMR 스펙트럼의 피크 분리로부터 확인할 수 있었다. 그 결과 수소결합에 관여하는 N-H의 방향이 거의 수직이라고 할 수 있는 83˚의 분자축 부분이 18˚의 고배향 분포를 이루면서 82% 존재하고, 또 나는 N-H의 결합방향이 60˚인 분자축 부분이 18% 존재하면서 62˚의 배향분포를 이룬다고 하는 시뮤레이션의 결과로 설명되어졌다.

• Natural Product Sciences
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• 제3권1호
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• pp.11-13
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• 1997

From the methanolic extract of defatted tender leaf of Camellia sinensis a new 4-hydroxy angular furocoumarin $C_{12}H_8O_5$, m.p. $212^{\circ}C$, was isolated using high-speed counter-current chromatographic technique. The structure of the compound was established as 4-hydroxy-2'-methoxy angular furocoumarin on the basis of physical methods viz. $^1H$ NMR, $^{13}C$ NMR and MS.

• Bulletin of the Korean Chemical Society
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• 제19권11호
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• pp.1244-1248
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• 1998

Ozonolysis reactions of cyclic olefins 1a-c and norbomene In in the presence of trifluoroactophenone 6 provided the corresponding cross-ozonides 7a-c and 7n. Further reactions of ozonides 7a-c and 7n with the independently prepared carbonyl oxide 11 gave diozonides of structure l0a-c and 10n. The ozonolysis of 1methylcyclopentene 12a and 1-methylcyclohexene 12b in the presence of trifluoroactophenone 6 provided exclusively ozonide 15 and 16 derived from capture of carbonyl oxide 13. All of the new ozonides have been isolated as pure substances and characterized by their 1H NMR and 13C NMR spectra.

• 한국축산식품학회지
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• 제41권2호
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• pp.312-323
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• 2021

The purpose of this study was to use 1H nuclear magnetic resonance (1H NMR) to quantify taste-active and bioactive compounds in chicken breasts and thighs from Korean native chicken (KNC) [newly developed KNCs (KNC-A, -C, and -D) and commercial KNC-H] and white-semi broiler (WSB) used in Samgye. Further, each breed was differentiated using multivariate analyses, including a machine learning algorithm designed to use metabolic information from each type of chicken obtained using 1H-13C heteronuclear single quantum coherence (2D NMR). Breast meat from KNC-D chickens were superior to those of conventional KNC-H and WSB chickens in terms of both taste-active and bioactive compounds. In the multivariate analysis, meat portions (breast and thigh) and chicken breeds (KNCs and WSB) could be clearly distinguished based on the outcomes of the principal component analysis and partial least square-discriminant analysis (R2=0.945; Q2=0.901). Based on this, we determined the receiver operating characteristic (ROC) curve for each of these components. AUC analysis identified 10 features which could be consistently applied to distinguish between all KNCs and WSB chickens in both breast (0.988) and thigh (1.000) meat without error. Here, both 1H NMR and 2D NMR could successfully quantify various target metabolites which could be used to distinguish between different chicken breeds based on their metabolic profile.