• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.23-23
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• 2022

Rapeseed (Brassica napus L.) is one of the most valuable oilseed crop in the world. It is widely used in various industries, such as food, animal feed, energy and chemical industries. In order to improve the industrial requirements for rapeseed, useful agronomic characteristics (higher yields and disease resistance etc.) and modified oil traits (fatty acid composition and fat content) are important in rapeseed. However, Korea has limiting genetic resources of novel traits in rapeseed. In this research, novel rapeseed mutant genotypes by mutation breeding was developed. The mutant lines were generated by the treatment of the seeds of the original cultivar 'Tamra' with 700 Gy of gamma-ray (⁶⁰Co). Mutants showing varied in flowering time, crude fat content, seed yield and fatty acid content that exhibited stable inheritance of the mutated characteristics from M₅ to M₇ generations were selected. We investigated genetic variation using SNPs identified from GBS analysis in rapeseed mutant lines derived from the gamma-ray, and interactions between the major agronomic and the oil traits. Significantly associated SNP loci were explored along with candidate genes using SNPs obtained by GBS analysis. As a results of association mapping, a total of 322 SNPs were significantly associated with agronomic traits (155 SNPs) and oil traits (167 SNPs). A total of 70 genes were annotated from agronomic characteristics SNPs; among them 7 genes significantly enriched in developmental process, and a total of 70 genes were annotated from crude fat content and fatty acid compositions SNPs; among them, 11genes were significantly enriched in biosynthetic process. These results could be used for the selection of rapeseed cultivar with enhanced qualities and potential economic benefits.

• 한국식품영양과학회지
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• 제33권1호
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• pp.22-27
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• 2004

인진쑥의 방사선에 의한 산화적 손상에 대한 DNA 방어효과를 확인하기 위하여 마우스 림프구에서 단세포전기영동법과 CHO 세포에서 미소핵 형성 시험 그리고 마우스의 간과 흉선 조직의 DNA에서 8-OHdG의 형성정도를 관찰하였다. 그 결과 단세포전기영동법 및 미소핵 형성 시험에서는 50 $\mu\textrm{g}$/mL의 농도에서 가장 높은 DNA 손상 억제 효과를 나타내었으며, 8-OHdG 형성 정도는 간과 흉선 모두 30 mg/kg 농도 투여군에서 높은 방어효과를 나타내었다. 이상의 결과에서 인진쑥 추출물은 DNA 상해를 효과적으로 방어하였고 특히, 기존에 알려진 방사선 방호물질에 비하여 독성이 적은 천연물이라는 관점에서 방사선 방어제로 적용이 가능할 것으로 사료된다.

• 한국식품과학회지
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• 제5권2호
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• pp.101-107
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• 1973

비 식용 해조로부터 식용 및 사료용 효모를 제조하기 위하여 다음의 결과를 얻었다. 1) 해조 탄수화물 자화 능력이 강한 효모 균주 Y-2503, J-4-2 를 해조 퇴적물로부터 분리하였다. 2) 분리된 효모 균주는 galactose, sucrose, glucose 의 발효력은 있었으나 maltose 와 mannit 의 발효력은 없었으며 mannit 를 자화시킬 수 있었다. 3) $1{\sim}2%$ NaCl 농도는 효모 증식에 별 영향이 없었으며 최적 pH 는 $4{\sim}5$ 였다. 4) 해조 분해물에서 pH $4{\sim}5$ 일때 침전물을 제거한 배양액에 있어서는 $(NH_4)_2SO_4$, $NaH_2PO_4$ 의 첨가가 당질의 자화와 효모 수량을 증가시켰다. 5) $Co^{60}$ gamma ray 조사(dose rate : 1 Mrad/hr. BNL shipboard irradiator)는 해조의 탄수화물 및 질소 화합물의 분해를 별로 증가시키지 못하였으나 효모 수량은 상당히 증가되었다. 6) Jar fermentor에서 효모를 배양한 결과 건물 해조에 대하여 $7{\sim}8%$의 건물 효모를 생산할 수 있었다.

• 한국식품과학회지
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• 제30권4호
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• pp.934-940
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• 1998

Kochujang, fermented hot pepper soybean paste, was prepared by traditional method, and irradiated with gamma ray of $^{60}Co{\;}up{\;}to{\;}15{\pm}1.5{\;}kGy$ for confirming main factor of kochujang fermentation. Nonirradiated (control) and irradiated samples kochujang were fermented at $25^{\circ}C$ and analyzed biochemical properties including enzyme activities and viable cell count during fermentation for 60 days. The total viable count in irradiated kochujang decreased to $10^4{\;}CFU/g$ which was $10^8{\;}CFU/g$ in the control. Because of a little changing enzyme activities of ${\alpha}{\cdot}{\beta}{\;}amylase$ and acid neutral protease by irradiation at the above level, amino type nitrogen which is the main quality reference of kochujang was comparable to the control. By irradiation, gas production was completely stopped which is one of biggest problems during distribution of kochujang. Total volume of gas produced at $25^{\circ}C$ from the control kochujang was 453 mL/100 g which was composed of over 90% of $CO_2$. The odor of irradiated kochujang was inferior to the control which seemed to be related to reduced microbial populations.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2001년도 추계종합학술대회
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• pp.644-647
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• 2001

이 연구에서는 P-MOS 커패시터에 Co $u^{60}$-${\gamma}$선을 조사한 후 조사선량 및 산화막 두께에 따른 전하의 거동을 고찰하고자 1[MHz]의 고주파 신호에서 정전용량-전압(C-V) 특성 및 유전손실계수-전압(D-V)특성을 측정하였다. C-V 특성에서 플랫밴드 전압과 문턱전압을 구하여 이들 파라메타와 D-V 특성의 피크와의 관련성을 검토하였다. C-V 특성이 P-MOS 커패시터의 정상상태의 전하의 거동 및 계면 상태특성을 해석하기가 편리하고 D-V 특성은 C-V 특성보다 산화막 내부의 공간전하분포와 계면상태의 밑도 등을 더 명확하게 파악할 수 있으며 산화막내 캐리어의 전도철상에 관한 미시적 전하 거동의 고찰에도 편리함이 확인되었다.

• 식물조직배양학회지
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• 제27권3호
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• pp.203-211
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• 2000

흰독말풀의 tumor조직에서 유도한 tumor callus로부터 scopolamine의 생산을 극대화하기 위한 연구를 수행하였다. Tumor callus의 생장률을 증가시키기 위해서는 초기접종량을 배지의 2%로 하고 배양기간은 4주가 효과적이다. 그래서 만일 이단계배양을 추진하려면 배지교체의 시기는 3주가 가장 적당한 것으로 확인되었다. Scopolamine의 생성을 위한 광의 효과를 알아본 결과, 암상태보다 광상태가 callus생장 및 scopolamine 함량 증가에 효과적이었다. Scopolamine 함량 증가의 최적 광량은 16$\mu$mo1 m$^{-2}$ s$^{-1}$, 광질은 적색파장 그리고 연속광보다 장일조건 (16/8)의 광주기에서 tropane alkaoid인 scopolamine합성이 현저히 촉진되었다. 질소원으로는 산화형 질소인 NO$_{3}$$^{-}$ 를 4 g/L로 증가시키는 것이 가장 효과적인 것으로 확인되었다. Scopolamine의 함량을 증가시키기 위한 elicitor 공급은 10 mg/L chitosan과 15 mg/L yeast추출물이 가장 효과적이었으며, 전구물질로는 0.2 mM tropine과 0.3 mM tropic acid가 가장 우수한 것으로 확인되었다. 이러한 결과를 종합해 보면, 흰독말풀 tumor callus에서 scopolamine 대량생산을 추진할 경우 3주 동안 광상태와 무기이온 조성을 균형 있게 조절하여 생장을 최대로 증가시킨 후, 1주 동안 scopolamine생산을 위해 elicitor, precursors 등이 조합된 배지에서 biotransformation 시키는 것이 가장 효과적일 것으로 판단된다. 또한 tumor callus에 $^{60}$Co${\gamma}$-ray을 조사하여 3K, 4K및 6k tumorcallus를 확보하였으며, 이들로부터 유도한 teratoma는 형태적 변이를 나타내었다. Teratoma로부터 또 다시 새로운 teratoma-callus 세포주를 유도하여 현탁배양을 수행하였던 바, MS 기본 배지에서 1.0 mg/L 2.4-D과 0.5 mg/L kinetin을 조합하여 처리한 callus 의 액체배양에서 cell aggregation이 일어나지 않고 세포가 유리화 되면서 왕성한 생장을 보였다.

• 한국재료학회지
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• 제22권6호
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• pp.275-279
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• 2012

(3-mercaptopropyl)trimethoxysilane (MPTMS) was used as a silylation agent, and modified silica nanoparticles were prepared by solution polymerization. 2.0 g of silica nanoparticles, 150 ml of toluene, and 20 ml of MPTMS were put into a 300 ml flask, and these mixtures were dispersed with ultrasonic vibration for 60 min. 0.2 g of hydroquinone as an inhibitor and 1 to 2 drops of 2,6-dimethylpyridine as a catalyst were added into the mixture. The mixture was then stirred with a magnetic stirrer for 8 hrs. at room temperature. After the reaction, the mixture was centrifuged for 1 hr. at 6000rpm. After precipitation, 150 ml of ethanol was added, and ultrasonic vibration was applied for 30 min. After the ultrasonic vibration, centrifugation was carried out again for 1 hr. at 6000rpm. Organo-modification of silica nanoparticles with a ${\gamma}$-methacryloxypropyl functional group was successfully achieved by solution polymerization in the ethanol solution. The characteristics of the ${\gamma}$-mercaptopropyl modified silica nanoparticles (MPSN) were examined using X-ray photoelectron spectroscopy (XPS, THERMO VG SCIENTIFIC, MultiLab 2000), a laser scattering system (LSS, TOPCON Co., GLS-1000), Fourier transform infrared spectroscopy (FTIR, JASCO INTERNATIONL CO., FT/IR-4200), scanning electron microscopy (SEM, HITACHI, S-2400), an elemental analysis (EA, Elementar, Vario macro/micro) and a thermogravimetric analysis (TGA, Perkin Elmer, TGA 7, Pyris 1). From the analysis results, the content of the methacryloxypropyl group was 0.98 mmol/g and the conversion rate of acrylamide monomer was 93%. SEM analysis results showed that the organo-modification of ultra-fine particles effectively prevented their agglomeration and improved their dispensability.

• Journal of Radiation Protection and Research
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• 제47권1호
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• pp.16-21
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• 2022

Background: Epidemiological observations such as mental retardation, physical deformities, etc., in children besides different types of cancer in the adult population of the Malwa region have been reported. The present study is designed to get insight into the role of naturally occurring radioactive material (NORM) in causing detrimental health effects observed in the general population of this region. Materials and Methods: Deep soil samples were collected from different locations in the Malwa region. Their activity concentrations were determined using low-level background gammaray spectrometry. High efficiency and high purity germanium detector capped in a lead-shielded chamber having a resolution of 1.8 keV at 1,173 keV and 2.0 keV at the 1,332 keV line of ⁶⁰Co was used in the present work. Data were evaluated with Genie-2000 software. Results and Discussion: Mean activity concentrations of ²³⁸U, ²³²Th, and ⁴⁰K in deep soil were found to be 101.3 Bq/kg, 65.8 Bq/kg, and 688.6 Bq/kg, respectively. The mean activity concentration of ²³⁸U was found to be three and half times higher than the global average prescribed by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR). It was further observed that the activity concentration of ²³²Th and ⁴⁰K has a magnitude that is nearly one and half times higher than the global average prescribed by UNSCEAR. In addition, the radioisotope ¹³⁷Cs which is likely to have its origin in radiation fallout was also observed. It is postulated that the NORM present in high quantity in deep soil somehow get mobilized into the water aquifers used by the general population and thereby causing harmful health problems. Conclusion: It can be stated that the present work has been able to demonstrate the use of low background gamma-ray spectrometry to understand the role of NORM in causing health-related effects in a general population of the Malwa region of Punjab, India.

• Journal of Radiation Protection and Research
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• 제40권1호
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• pp.25-35
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• 2015

We analyzed the differential effects of histopathology, apoptosis and expression of radiation response genes after chronic low dose rate (LDR) and acute high dose rate (HDR) radiation exposure in spleen, lung and liver of rats. Female 6-week-old Sprague-Dawley rats were used. For chronic low-dose whole body irradiation, rats were maintained for 14 days in a $^{60}Co$ gamma ray irradiated room and received a cumulative dose of 2 Gy or 5 Gy. Rats in the acute whole body exposure group were exposed to an equal dose of radiation delivered as a single pulse ($^{137}Cs$-gamma). At 24 hours after exposure, spleen, lung and liver tissues were extracted for histopathologic examination, western blotting and RT-PCR analysis. 1. The spleen showed the most dramatic differential response to acute and chronic exposure, with the induction of substantial tissue damage by HDR but not by LDR radiation. Effects of LDR radiation on the lung were only apparent at the higher dose (5 Gy), but not at lower dose (2 Gy). In the liver, HDR and LDR exposure induced a similar damage response at both doses. RT-PCR analysis identified cyclin G1 as a LDR-responsive gene in the spleen of rats exposed to 2 Gy and 5 Gy gamma radiation and in the lung of animals irradiated with 5 Gy. 2. The effects of LDR radiation differed among lung, liver, and spleen tissues. The spleen showed the greatest differential effect between HDR and LDR. The response to LDR radiation may involve expression of cyclin G1.

• 한국정보통신학회논문지
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• 제20권10호
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• pp.1980-1986
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• 2016

본 논문에서는 KrF 레이저를 이용한 격자 공정 후 고온 어닐링 온도조건에 따른 감마방사선 영향을 분석하였다. 제작된 광섬유 브래그 격자는 게르마늄(Ge)이 첨가된 동일한 광섬유에 어닐링 온도를 달리하여 제작하였으며, $Co^{60}$ 감마선원을 이용하여 약 115 Gy/min의 선량률로 총선량 약 31 kGy 감마선을 조사하였다. 격자의 안정화를 위한 고온 어닐링 공정은 광섬유 브래그 격자의 방사선 민감도 변화에 영향을 주는 것으로 나타났다. 실험결과를 통하여, 각각 다른 온도(100, 150, $200^{\circ}C$)로 안정화시킨 광섬유 브래그 격자들은 고온에 노출될수록 방사선 민감도가 증가했으며, 어닐링 온도조건에 따라서 방사선에 의한 브래그 파장 변화는 2배 이상의 차이를 보였다.