• Title/Summary/Keyword: $^{15}N$ recovery

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Evaluation of Acid Digestion Procedures to Estimate Mineral Contents in Materials from Animal Trials

  • Palma, M.N.N.;Rocha, G.C.;Valadares Filho, S.C.;Detmann, E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.11
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    • pp.1624-1628
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    • 2015
  • Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01) of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p<0.01) interaction between sample type and nitric to perchloric acid ratio. Digestion solution of 2:1 v/v provided greater (p<0.01) recovery of calcium and phosphorus from bone samples than 3:1 and 4:1 v/v ratio. Different acid ratios did not affect (p>0.01) calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01). Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

The Antiemetic Effects of Intravenous Dexamethasone in Preventing Continuously Infused Epidural Morphine-related Nausea and Vomiting (Dexamethasone 정주가 경막외강 내 지속적 투여된 Morphine으로 인한 구역, 구토에 미치는 영향)

  • Koo, Bon-Nyeo;Yoon, Duck-Mi;Kim, Ji-Young;Kim, Jong-Chul;Nam, Yong-Taek
    • The Korean Journal of Pain
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    • v.14 no.2
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    • pp.176-180
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    • 2001
  • Background: The aim of our study was to evaluate the antiemetic effects of intravenous dexamethasone in preventing continuously infused epidural morphine-related nausea and vomiting. Methods: Twenty-seven patients requiring general anesthesia for gastrectomy were enrolled in a randomized, double-blinded, and placebo-controlled study. At the end of surgery, all patients received epidural morphine 3 mg and were connected to an epidural morphine infusion pump for 2 days in order to relieve postoperative pain. Before the morphine injection, the dexamethasone group (n = 12) received IV dexamethasone 10 mg, whereas the saline group (n = 15) received IV saline. The incidence of nausea & vomiting, pruritus, back pain and VAS scores were assessed in the recovery room, and at 24 h and 48 h postoperatively. Results: There was no significant difference in the total incidence of nausea and vomiting, pruritus, back pain or in the VAS scores. However, there was no vomiting and no back pain in the dexamethasone group. Conclusions: Intravenous dexamethasone did not significantly decrease the total incidence of nausea or vomiting in patients receiving continuous epidural morphine for postoperative pain control. However, IV dexamethasone appears to decrease the severity of nausea, vomiting and back pain.

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Effects of Korean red ginseng on T-cell repopulation after autologous hematopoietic stem cell transplantation in childhood cancer patients

  • Kyung Taek Hong;Yeon Jun Kang;Jung Yoon Choi;Young Ju Yun;Il-Moo Chang;Hee Young Shin;Hyoung Jin Kang;Won-Woo Lee
    • Journal of Ginseng Research
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    • v.48 no.1
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    • pp.68-76
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    • 2024
  • Background: Although the survival outcomes of childhood cancer patients have improved, childhood cancer survivors suffer from various degrees of immune dysfunction or delayed immune reconstitution. This study aimed to investigate the effect of Korean Red Ginseng (KRG) on T cell recovery in childhood cancer patients who underwent autologous hematopoietic stem cell transplantation (ASCT) from the perspective of inflammatory and senescent phenotypes. Methods: This was a single-arm exploratory trial. The KRG group (n = 15) received KRG powder from month 1 to month 12 post-ASCT. We compared the results of the KRG group with those of the control group (n = 23). The proportions of T cell populations, senescent phenotypes, and cytokine production profiles were analyzed at 1, 3, 6, and 12 months post-ASCT using peripheral blood samples. Results: All patients in the KRG group completed the treatment without any safety issues and showed a comparable T cell repopulation pattern to that in the control group. In particular, KRG administration influenced the repopulation of CD4+ T cells via T cell expansion and differentiation into effector memory cell re-expressing CD45RA (EMRA) cells. Although the KRG group showed an increase in the number of CD4+ EMRA cells, the expression of senescent and exhausted markers in these cells decreased, and the capacity for senescence-related cytokine production in the senescent CD28- subset was ameliorated. Conclusions: These findings suggest that KRG promotes the repopulation of CD4+ EMRA T cells and regulates phenotypical and functional senescent changes after ASCT in pediatric patients with cancer.

Fixation of Sericin of Silk Fabric by Epoxy Resin (Epoxy수지에 의한 견직물의 Sericin정착)

  • 문영배;남중희
    • Journal of Sericultural and Entomological Science
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    • v.26 no.2
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    • pp.16-25
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    • 1984
  • The sericin fixation of silk fabrics by epoxy resins was studied in the presence of aqueous salt solution in different solvents at the indicated temperature for the desired time. Heavy weight gains were obtained in the reaction with glycerol diglycidyl ether (EX-313) and ethylene glycol diglycidyl ether (EX-810) catalyzed by potassium thiocyanate in such solvents as carbon tetrachloride and p-chloroethylene. The obtained results were summarized as follows; 1) The tested resins were found similar in reaction behavior to silk fabric. The effect of fixation and weight gains was higher in EX-810 than EX-313. 2) The weight gains were increased with reaction time and temperature, and degumming ratio reached a constant value in 90 min at 70$^{\circ}C$. 3) The weight gains and the degumming ratio reached an equilibrium at 3-5% of epoxy concentration. 4) The weight gains were remarkably influenced and increased by the concentration of salt solution. The degumming ratio reached an equilibrium over 0.5N concentration of salt. 5) The weight gains were increased with the dipping time in gently-sloping. The degumming ratio reached an equilibrium over 15 min dipping. 6) The effect of sericin fixation of hydrophobic solvents, such as Carbon Tetrachloride, p-Chloroeth-ylene, Cyclohexane, Xylene and Toluene, was found suitable. 7) The effect of drying temperature was not remarkable on the weight gains and the degumming ratio. 8) There was a slight decrease in the moisture regain of sericin-fixed silk and it may be possible to maintain the moisture regain in the sericin-fixed silk by the epoxy resins. 9) The results on testing physical properties of sericin-fixed silk fabric were as follows; The crease recovery was almost not different from undegummed fabric, but inferior to degummed fabric. The tensile strength was improving in accordance with the effect of sericin fixation, either the elongation did.

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Comparison of Binocular Function in Normal Subjects and Convergence Insufficiency (정상안과 폭주부족안의 양안시기능 검사값 비교)

  • Shim, Hyun-Seog;Shim, Moon-Sik;Kim, Sang-Hyun
    • Journal of Korean Ophthalmic Optics Society
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    • v.15 no.3
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    • pp.287-291
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    • 2010
  • Purpose: The aim of this study was to compare binocular functions such as near point of convergence (NPC), AC/A ratios, heterophoria, accommodation, accommodation lag, positive relative accommodation (PRA), negative relative accommodation (NRA), positive relative convergence (PRC), negative relative convergence (NRC) in normal subjects and convergence insufficiency. Methods: 86 subject (male n=45, female n=41, mean $age{\pm}SD=23.27{\pm}2.85$ years) without amblyopia, strabismus, and ocular pathology were studied. Forty three patients each group were classified as normal subjects and convergence insufficiency group based on AC/A ratio and far and near phoria. Binocular function of the two groups was measured using phoropter. r. Results: The values between normal subjects and convergence insufficiency were 5.71 cm and 7.07 cm for NPC, 5.28 and 2.81 for Heterophria AC/A, 0.92 exo/3.36 exo and 2.17 exo/10.84 exo for far and near phoria, 15.49/23.30/13.30 and 13.50/20.02/9.09 for PRC, respectively. However, there were no significant differences between normal subjects and convergence insufficiency for accommodative amplitude, accommodative lag, PRA, NRA, BI vergence test, BO vergence test, NRC. Conclusions: Both groups were a significant difference for cover test, NPC, Heterophria AC/A, far and near phoria, break point of PRC, and recovery point of PRC.

Effects of Aging and Soil Texture on Composting of Diesel-Contaminated Soil (디젤오염기간 및 토성이 오염토양 콤포스팅 처리에 미치는 영향)

  • Choi, Jung-Young;Namkoong, Wan;Park, Joon-Seok;Hwang, Eui-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.2
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    • pp.132-139
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    • 2002
  • This study was carried out to investigate the effects of aging and soil texture on composting of diesel-contaminated soil. The soils used for this study were silt loam and sand. Target contaminant, diesel oil, was spiked at 10,000mgTPH/kg of dry soil. Aging times of diesel-contaminated soils were 15days and 60days, respectively. Fresh diesel-contaminated soil was also investigated. Moisture content was controlled to 70% of soil field capacity. Mix ratio of soil to sludge was 1:0.3 as wet weight basis. Temperature was maintained at $20^{\circ}C$ Volatilization loss of TPH was below 2% of initial concentration. n-Alkanes lost by volatilization were mainly by the compounds of C10 to C17. Diesel in contaminated soil was mainly removed by biodegradation mechanism. First order degradation rate constant of TPH in sandy soil was ranged from 0.081 to 0.094/day, which is higher than that in silt loam(0.056-0.061/day). From fresh to 60day-aged soils, there was little difference of TPH biodegradation rate between the soils. Carbon recovery ranged from 0.61 to 0.89. TPH degradation rate was highly correlated with $CO_2$ production rate.

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The Bio Gasification technology of organic waste using vertical multistage anaerobic digester (다단 수직형 혐기성 소화조를 이용한 유기성 폐기물 바이오가스화 기술)

  • Lee, Jong Hak;Eum, Young-Kyoung;Kim, Youngnoh
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.3
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    • pp.15-20
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    • 2012
  • Processing of organic waste is converted to anaerobic digestion for environmental friendly and sustainable energy recovery and reduction of sludge. In this study, ECOPAD (ECOdays' Plug-flow Anaerobic Digster)design for a high solids content and high organic matter content were used to investigate an applicability and efficiency of food waste treatment and poultry wastewater treatment. Case-by-case analysis of treatment efficiency of ECOPAD using food waste of city "P" and city "S". Volatile Solids basis organic removal efficiency of city "P" and city "S" was 84% and 88% respectively. And, the content of methane (P City: 70%, S City: 71%) was measured similarly. In the case of poultry waste bio-gas production was measured to $1.6Nm^3/kg$-VSrem, and methane content was measured to 69%.

Analysis of environmental benefit of wood waste recycling processes (폐목재 자원화 방법 환경편익 분석)

  • Kim, Mi Hyung;Hong, Soo Youl;Phae, Chae Gun;Koo, Ja Kong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.2
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    • pp.15-19
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    • 2012
  • Wood wastes could be renewable resources by recycling as particleboard manufacturing or energy production. Particle board is the most common item of wood waste recycling and energy production from wood wastes has highlighted for energy recovery to reduce greenhouse gas generation in recent years. The aim of this study was to evaluate the environmental benefits of the processes for particle board manufacturing and energy production. The functional unit was one ton of wood wastes and the environmental impact was analyzed by life cycle assessment methodology. The result was that 112kg of carbon dioxide equivalent was produced from particle board manufacturing process and 382kg of carbon dioxide equivalent was produced from combined heat and power generation process. The concept of temporary biomass carbon storage was to applied to this study.

Comparison of Mechanical Stability between Fibular Free Flap Reconstruction versus Locking Mandibular Reconstruction Plate Fixation

  • Chung, Jae-Hyun;Yoon, Eul-Sik;Park, Seung-Ha;Lee, Byung-Il;Kim, Hyon-Surk;You, Hi-Jin
    • Archives of Craniofacial Surgery
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    • v.15 no.2
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    • pp.75-81
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    • 2014
  • Background: The fibular free flap has been used as the standard methods of segmental mandibular reconstruction. The objective of mandibular reconstruction not only includes restored continuity of the mandible but also the recovery of optimal function. This paper emphasizes the advantage of the fibular free flap reconstruction over that of locking mandibular reconstruction plate fixation. Methods: The hospital charts of all patients (n=20) who had a mandibular reconstruction between 1994 and 2013 were retrospectively reviewed. Eight patients had plateonly fixation of the mandible, and the remaining 12 had vascularized fibular free flap reconstruction. Complications and outcomes were reviewed and compared between the 2 groups via statistical analysis. Results: Overall complication rates were significantly lower in the fibular flap group (8.3%) than in the plate fixation group (87.5%; p =0.001). Most (7/8) patients in the plate fixation group had experienced plate-related late complications, including plate fracture or exposure. In the fibular flap group, no complications were observed, except for a single case of donor-site wound dehiscence (1/12). Conclusion: The fibular free flap provides a more stable support and additional soft tissue support for the plate, thereby minimizing the risk of plate-related complications. Fibular free flap is the most reliable option for mandibular reconstruction, and we believe that the flap should be performed primarily whenever possible.

Livestock Manure Nutrients Flow Analysis of Integrated Crop-Livestock Farming Model Reflecting the Regional Characteristics (지역특성을 고려한 경축순환농업 모형의 가축분뇨 양분 흐름분석)

  • Lee, Joon Hee;Choi, Hong Lim
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.2
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    • pp.36-46
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    • 2015
  • Integration of crop-livestock farming has been a problem-solving mode for abatement of environmental pollution and recovery of resources in recent years. The objectives of this study were 1) to suggest the customized integration of crop-livestock farming model reflecting the regional characteristics through in-depth analysis of case study and 2) to analyze the livestock nutrients flow in terms of three primary elements as nitrogen(N), phosphorous(P), and potassium(K). The personal interview and survey were carried out in 2012 for a total of 161 farms from four different regions(NS, NW, JJ, YC) in South Korea. The mass balance analysis was used to suggest and evaluate the models for two sites(JJ and YC). The results showed that NS and NW sites produced relatively more livestock manure than the sites of YC and JJ because of the regional differences in livestock numbers and urbanization. The models were suggested for the site JJ and site YC, and 'two track model(energy and resource recovery)' and 'dispersal type model' were assigned respectively. For the nutrient flows, the releasing P and K with new models had increased up to 7%, while N release had decreased down to 15% in both YC and JJ sites compared to the present treatment system. Estimated value showed that there was oversupply of N (719 ton/yr) and $P_2O_5$ (1,269 ton/yr) in YC and deficiency of N (671 ton/yr) and excessive $P_2O_5$ (32 ton/yr) in JJ respectively. Therefore, P runoff has to be considered an eutrophication occurs in rural small stream when an integration of crop-livestock farm system is applied into both sites.