• Title/Summary/Keyword: $\delta$-ALAD

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Effects of Polyamines on Chlorophyll and Protein content, and $\delta$-Aminolevulinate Dehydratase Activity in Greening Mung Bean Cotyledons (녹두자엽에서 엽록소 및 단백질함량과 $\delta$-Aminolevulinate Dehydratase활성에 미치는 Polyamine의 영향)

  • Jung-Hee Hong
    • Journal of Environmental Science International
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    • v.2 no.4
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    • pp.255-270
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    • 1993
  • Effects of polyamines on chlorophyll and protein content, and $\delta$-aminolevulinate dehydratase(ALAD) activity were investigated during the greening of mung bean cotyledons. Polyamines stimulated chlorophyll formation in greening cotyledons, and this effect was enhanced by KCl. The changes in protein content were similar to the changes for chlorophyll content. The excision entailed an increase in ALAD activity. Then a decrease appeared after 48 h incubation on water in the dark. It was more precocious in the light, but was accelerated when the cotyledons were illuminated after a dark preincubation. Putrescine had little effect on ALAD activity in the dark. In the light, putrescine prevented the decrease in ALAD activity and enhanced this activity when a dark preincubation preceded illumination. KCl had a slight stimulating effect in the dark, but was uneffective in the light. The combination putrescine+KCl was devoid of stimulating effect. The results obtained suggest that plastid development of mung bean cotyledons during greening was affected by polyamines and light and that polyamines may play a role in the regulation of plastid development.

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Rhizobium meliloti 102F51 Mutants Defective in Heme Synthesis (Heme 합성특성이 다른 Rhizobium meliloti 102F51 Mutant의 선별)

  • 최영주;정원화;김경수;신평균;조무제
    • Korean Journal of Microbiology
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    • v.24 no.2
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    • pp.98-105
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    • 1986
  • Rhizobium meliloti 102 F 51, the symbiotic partner of alfalfa, was mutagenized with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and UV-irradiation. Three group of mutants which form white, white-pink and red nodules were selected. The adetylene reduction activity, nodulation activity, amount of heme synthesis during the nodulation, and ${\delta}-aminolevulinic$ acid synthetase (ALAS) and ${\delta}-aminolevulinic$ acid dehydratase (ALAD) activities in free living rhizobia and bacteroid states of the each group of mutants were compared. The mutants forming white nodules showed lower acetylene reduction activity compared to those of red nodule forming mutants. The two key enzymes for the heme synthetic pathway, ALAS and ALAD activities of the mutants forming red nodules was much higher than those of the mutants forming white nodules in bacteroid state, however no significant difference was observed in free living state. In the nodules the ALAS was detected only in bacteroid fraction, while ALAD was detected both in bacteroid and plant fraction. ALAS was dramatically increased with the heme synthesis during the nodulation, while ALAD was decreased in plant fraction but slight increase was observed in bacteroid fraction.

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Effect of Dietary Selenium on $\delta$-Aminolevulinic Acid Dehydratase Activity in Lead Poisoned Rats (식품 Selenium이 납중독된 흰쥐에 있어서 $\delta$-Aminolevulinic Acid Dehydratase 활성에 미치는 영향)

  • 방진숙
    • Journal of Nutrition and Health
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    • v.24 no.6
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    • pp.526-533
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    • 1991
  • The effect of dietary selenium on the activity of $\delta$-aminolevulinic acid dehydratase (ALAD) inhibited by the administration of lead were investigated in rats. The levels of dietary lead in the acetate form were 0(contro)200, 1, 000, 2, 000 and 5, 000ppm. Except control group four-level of lead diet groups were again subdivided into two depending on with and without 0.5ppm selenium supplementation. Sixty-three 40-day-old male Sprague-Dawley rats weighing 141$\pm$5g were distributed into total of nine diet groups according to RCB design and fed ad libidum for 4 weeks. Lead dietary groups did not show any significnat difference in food intake from the control group. Food efficiency and weight gain were lower in 2.000ppm and 5, 000ppm lead groups but not found in selenium supplemented ones. Hemoglobin contents hematocrit values ALAD activities in blood were significantly decreased and urinary aminolevulinic acid(ALA) excretion increa-sed with increasing dietary lead levels but partly restored by selenium supplementation. however only in 200, 1, 000 and 2, 000ppm dietary lead groups. On the other hand the hepatic ALAD activites of all four lead groups were recovered 19-30% from suppression by selenium supplementation. It was concluded that selenium administration alleviated lead toxicity in rats.

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The Influence of Ascorbic Acid and Methionine on the $\delta$-Aminolevulinic Acid Dehydratase Activity in Lead Poisoning. (납중독에 있어서 $\delta$-Aminolevulinic Acid Dehydratase 활성에 미치는 Ascorbic Acid 및 Methionine의 효과에 관한 연구)

  • 윤혜정
    • YAKHAK HOEJI
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    • v.19 no.1
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    • pp.21-29
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    • 1975
  • The activity of $\delta$-aminolevlinic acid dehydratase (ALAD) in red cell of rabbit inhibited by addition of $Pb(Ac)_{2}$(50mg/kg) to rabbit caused to diminish completely the ALAD activity in blood within shr. Pretreatment of ascorbic acid and methionine decreased the increment of $\delta$-aminolevulinic acid output in urine by lead poisoning.

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Relationship on the lead exposure indices and symptoms by ALAD genotype in lead worker (연 노출 근로자들의 ALAD genotype에 따른 연 노출지표 및 증상과의 관련성)

  • Ahn, Kyu Dong;Lee, Jong Chun;Cho, Kwang Sung;Kim, Jin Ho;Lee, Sung Soo;Lee, Byung Kook
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.11 no.2
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    • pp.111-117
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    • 2001
  • A cross-sectional study was performed to evaluate associations between lead biomarkers, lead-related symptoms, and ${\delta}$-aminolevulinic acid dehydratase (ALAD) genotype among 598 lead workers and 144 control office workers in storage battery industries, secondary smelting and litharge making industries. Lead inhibits the second enzymes, ALAD, in the heme synthesis pathway. ALAD gene, which codes for one of three isozymic proteins (termed ALAD1-1, ALAD1-2, and ALAD2-2), seems to modify the toxicokinetics of lead. The result as follows; The percents of total workers whose genotype of ALAD1-1 and ALAD1-2 were 88.4% and 11.6%, respectively. The zinc protoporphyrin in blood (ZPP) and ${\delta}$-aminolevulinic acid in urine (ALAU) of lead workers with ALAD1-2 were significantly lower than those of lead workers with ALAD1-1, but there were no significant difference between two genotype for blood lead, age, and work duration. The proportion of ALAD1-2 genotype in control office workers was 13.2%. The proportions of ALAD1-2 genotype of lead workers were 14.0%(their mean air lead level below $0.024mg/m^3$), 10.4%($0.025-0.049mg/m^3$), 11.8%($0.050-0.099mg/m^3$), and 9.4%(above $0.100mg/m^3$), respectively. In the logistic analysis of 15 lead related symptoms, 'arthralgia'(S7) symptom of ALAD1-2 was significantly lower (OR=0.481; 95% CI=0.248-0.932) than that of ALAD1-1, but 'feeling of irritation'(S11) of ALAD1-2 was significantly higher(OR=1.636; 95% CI=1.035-2.586) than that of ALAD1-1 after controlling possible confounder (blood lead, work duration, smoking and drinking habit).

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A Study on the Preventive Effect of Kam Doo Decoction on the Subacute Lead Toxicity in Rats (흰쥐에서 아급성 연독성에 대한 감두탕의 예방효과에 관한 연구(II) - 소변 및 혈액에 미치는 영향을 중심으로 -)

  • 이선동;이용욱;방형애
    • Journal of Environmental Health Sciences
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    • v.20 no.1
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    • pp.83-95
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    • 1994
  • This study was performed to investigate the preventive effect of KDD against lead toxicity. KDD of 133, 266, 532 and 1,064 mg/kg were administered twice to the rats of Sprague-Dawley strain and then 300 mg/kg lead acetate was given to times, respectively. 1. The $\delta$-ALAD concentration in the urine showed 10.6 ~16.4 mg/kg in the control group indicated statistical significance for the experimental group II, III, IV, V (p<0.05). Also, the Coproporphyrin concentration had 0.119 ~ 0.226 $\mu$g/ml in the control group indicated statiscial significance for the experimental group V of 10 weeks (p<0.05). 2. The $\delta$-ALAD concentration in the blood showed 13.28 ~ 16.08 ALAD unit in the control group indicated statistical significance for the experimental group I (Pb 300 mg/kg) of 6 and 8 weeks, for the experimental group III, IV of 8 and 10 weeks, and for the experimental group V of 4 weeks (p<0.05). The $\delta$-ALAD concentration of experimental group I (Pb 300 mg/kg) group was inclined to decrease during the experiment period. The $\delta$-ALAD concentration of experimental group I (Pb 300 mg/kg) showed statistical significance for the experimental group II, III, IV, V of 6, 8 and 10 weeks. But, there was no statistical significance in the concentration change of hemoglobin, RBC, WBC, hematocrit, Ca, protein among the experimental groups. In conclusion, this study revealed the preventive effect of KDD against lead toxicity and its mechnism inferred to facilitate lead excretion in urinary following hinderance of lead absorption in the gastric-intestine and organs.

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Iron Nutritional Status by ALAD Genotype and Intervention Study for Rural Area Residents (농촌 여성들의 ALAD 유전형질별 철분영양상태와 철분제 섭취에 따른 영양상태의 변화)

  • Kim, Hee-Seon;Kim, Min-Kyung;Kim, So-Hee;Lee, Sung-Soo;Lee, Byung-Kook
    • Korean Journal of Community Nutrition
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    • v.11 no.6
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    • pp.771-778
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    • 2006
  • Previous studies have suggested that delta-aminolevulinic acid dehydratase (ALAD) phenotype differently affect mineral metabolism. The objective of this study was to determine the effectiveness of 6-month iron supplementation as syrup of NaFeEDTA in improvement of iron status according to ALAD genotype. One hundred thirty adult women living in rural areas of Asan were provided NaFeEDTA syrup once a week for 6 months at the dose of 64mg Fe/week. Three hundred control subjects were observed during the study period. Fasting blood was obtained for analyzing hemoglobin (Hb) and zinc protophorphyrin (ZPP) and serum was analyzed for ferritin, iron and total iron capacity (TIBC) levels before and after iron supplementation. Ninety percent of ALAD 1-1 (ALAD1) and 10% of ALAD 1-2 (ALAD2) genotype were observed in the control group. However in the intervention group, 98% showed ALAD1 while only 2% was ALAD2, which is significantly lower proportions of ALAD2 compared to the control group (p<0.01). The iron status of Intervention group significantly improved except for ferritin and TIBC regardless or ALAD genotype, while the control group did not show any changes in iron status except for ZPP. ZPP concentration of the control group significantly increased in both ALAD1 and 2 while the intervention group showed significantly decreased ZPP after supplementation in ALAD1. Iron supplementation in the form of NaFeEDTA seems to be effective in reduction of ZPP levels although ALAD2 did not show significant changes due to the small number. However, it is difficult to make a conclusion from these results, and more specified further investigation is needed with more participants.

Effects of Repeated Exposure to Pb Acetate on Hematopoietic Function, Testis and Kidney in Male Rats (Pb acetate의 13주 반복투여가 랫드의 조혈기능, 정소 및 신장에 미치는 영향)

  • 홍충만
    • Toxicological Research
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    • v.17 no.4
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    • pp.309-316
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    • 2001
  • Male Sprague Dawley rats were exposed to 0, 0.04, 0.2, and 0.8% Pb acetate in drinking water for 13 weeks and fed a commercial diet. Dose-related adverse effects observed at the end of the Pb acetate exposure in the drinking water were as follows: decrease in body weight gain, decrease in hemoglobin, hematocrit(HCT), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH), increase in serum glucose, decrease in serum testosterone, increase in lead accumulation and $\delta$-ALA release in urine, and decrease in $\delta$-ALAD activities DNA content and histopathlogy (intranuclear inclusion body in kidney proximal tubule cell). Taken together, repeated exposure of lead acetate induced toxicities in hematopoietic system, especially testis and kidney.

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Studies on the Bis (amino-alkyl-oxo) Oxalate Derivatives As a Antidote of Lead poisoning (Bis (\beta$-amino-alkyl-oxo) oxalate 유도체의 납 중독 해독에 관한 연구)

  • 최선아;유미례;노영수;정성현;정세영
    • Environmental Analysis Health and Toxicology
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    • v.4 no.3_4
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    • pp.1-6
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    • 1989
  • The effects of his ($\beta$-amino-ethyl-oxo) oxalate and bis ($\alpha$-amino-methyl-oxo) oxalate on the toxicity of lead acetate in rast were examined. Rats were given intraperitoneally at the dose of lead acetate 45 mg/kg. The exposure of lead acetate showed the 70% decrease of ALAD ($\delta$-amino levulinic acid dehydratase) activity in red blood cell. In vivo 122 mg/kg administration of bis ($\beta$-amino-ethyl-oxo) oxalate and bis ($\alpha$-amino-methyl-oxo) oxalate showed the 50% increase of ALAD activity, whereas 149 mg/kg administeration of Ca-EDTA had no effect. In vitro, the same results were obtained. Both compounds had hemolytic activity at concentrations higher than that required for showing the 50% ALAD activity increase in vivo.

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Study on the Toxicities of Polychlorinated Biphenyls, Phenol and Biphenyl (Polychlorinated Biphenyls, Phenol 및 Biphenyl의 독성학적 연구)

  • 홍사욱;정규혁
    • Environmental Analysis Health and Toxicology
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    • v.1 no.1
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    • pp.61-70
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    • 1986
  • The effect of polychlorinated biphenyls (PCB), phenol and biphenyl on the body, liver and kidney weights, and the activity of $\delta$-aminolevulinic acid dehydratase (ALAD), and the contents of microsomal cytochrome P-450, and hematocrit, TBAvalue. PCB (200 mg/kg), phenol (200 mg/kg), biphenyl (200 mg/kg), and biphenyl (100 mg/kg) added phenol (100 mg/kg) was treated orally to Sprague-Dawley rats for 3 days. In all treated groups, the body weights were decreased, while the weights of liver and kidney were increased in comparison with that of control group. The activity of $\delta$-ALAD was increased and hematocrit was decreased in PCB treated group, on the contrary biphenyl treated group was appeared opposite direction. The contents of microsomal cytochrome P-450 and concentration of protein were increased in all treated group. In biphenyl treated group and phenol treated group, TBA value was increased in both groups.

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