• 제목/요약/키워드: $\beta-glucuronidase$

검색결과 254건 처리시간 0.028초

Porcine Leukocyte Lysosomal Hydrolase의 정제에 관(關)한 연구(硏究) (Purification of Porcine Leukocyte Lysosomal Hydrolases)

  • 조무제
    • Applied Biological Chemistry
    • /
    • 제20권1호
    • /
    • pp.136-141
    • /
    • 1977
  • 돼지 백혈구(白血球) Iysosome의 n-butanol 추출물(抽出物)로 부터 DEAE-cellulose, Sephadex c-200 column chromatography 및 Polyacrylamide gel electrophoresis 로서 acid phosphatase, aryl sulfatase, ${\beta}-glucuronidase$로서 및 cathepsin D를 분리(分離)정제하고 그 성질을 조사하였다.

  • PDF

Effects of Lactic Acid Bacteria on intestinal Microbial Enzyme Activity and Composition in Rats Treated with Azoxymethane

  • Sang-Myeong;Lee, Wan-Kyu
    • Journal of Microbiology
    • /
    • 제39권3호
    • /
    • pp.154-161
    • /
    • 2001
  • In recent years, colon cancer has been reported to be one of the most important causes of cancer morbidity and mortality in Korea. Epidemiological and experimental studies suggest that lactic acid bacteria (LAB) used to ferment dairy products inhibits colon carcinogenesis. The present study was designed to determine whether the colon cancer inhibitory effect of LAB (Bifidobacterium longum Hy8001; Bif and Lactobacillus acidophilus HY2l04; Lac) of Korean origin, is associated with intestinal microflora composition and certain enzyme activity in rats treated with azoxymethane (AOM). At five weeks of age, SD rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Oral administration of lactic acid bacteria cultures were performed daily until the termination of the study. Two weeks later all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once weekly for 2 weeks. Every two weeks for 10 weeks, five of the rats in each group were randomly chosen for fecal specimen collection. The fecal specimens were used for assay of $\beta$-glucuronidase and nitroreductase, and analysis of intestinal microflora composition. The activity of $\beta$-glucuronidase which plays an important role in the production of the carcinogenic metabolite of azoxymethane was remarkably increased in the AOM alone group after AOM injection and maintained the high level during the experiment. However, LAB inhibited the AOM-induced increase in $\beta$-glucuronidase activity. Nitroreductase activity decreased by 30-40% in LAB treated groups in comparison with that of the AOM alone group. The results of the present study suggest that LAB inhibits colon carcinogenesis by modulating the metabolic activity of intestinal micro-flora and improving the composition of intestinal microflora.

  • PDF

생약복합제제중 보기제(補氣劑)의 혈청성분 및 장내미생물에 대한 작용 (Effect of Combined Preparations of Crude Drugs used as Energy Invigorator on Serum Components and Intestinal Bacteria in Mice)

  • 김남재;김동현;박종백;홍남두
    • 생약학회지
    • /
    • 제24권3호
    • /
    • pp.235-243
    • /
    • 1993
  • We have concerned the action of traditional herbal medicine (THM) to relate with longevity, aging and immune, etc. Espically, we have studied on the role of intestinal bacteria on THM such as Nokyongdaebo-Tang, Bojungikgi-Tang, Ikgibohyul-Tang, Bobyulansin-Tang and Bojanggunbi-Tang. Samples of the p.o. administration of 2 g/kg for 2 weeks did not find the change transaminase activities in mice. But, Nokyongdaebo-Tang and Bojanggunbi-Tang elevated the serum glucose levels and Bojungikgi-Tang elevated the total cholesterol levels in mice. ${\beta}-Glucuronidase$ activities in mice faeces are decreased by the treatment of Nokyongdaebo-Tang, Bojungikgi-Tang, Ikgibohyul-Tang and Bojanggunbi-Tang, but increased by the treatment of Bohyulansin-Tang. Number of lactic acid bacteria in mice faeces are increased by the treatment of Nokyongdaebo-Tang, Bojungikgi-Tang, lkgibohyul-Tang, Bohyulansin-Tang and Bojanggunbi-Tang. We recognized that ${\beta}-glucuronidase$ activities and the number of lactic acid bacteria were remarkably decreased by the pretreatment of antibiotics in mice. Nokyongdaebo-Tang and Ikgibohyul-Tang at the p.o. administration of 2 g/kg significantly increased the ${\beta}-glucuronidase$ activities and Bojungikgi-Tang and Bohyulansin-Tang remarkably increased the number of lactic acid bacteria in antibiotics pretreated mice.

  • PDF

식이섬유 급원 첨가하에서의 식이제한이 흰주의 장내 환경에 미치는 영향 (The Influence of Dietary Fiber on Intestinal Environment in Rats Controlled by Food Restriction)

  • 강어진
    • 한국식품영양학회지
    • /
    • 제11권4호
    • /
    • pp.460-465
    • /
    • 1998
  • The effects of food restriction on the fecal microflora, moisture, pH, indole, ${\beta}$-glucosidas, and ${\beta}$-glucuronidase in rate were studied for 4 weeks. Four groups of rats for feeding was allocated to the following experimental trials : (1) control containing 1% cellulose, (2) control with food restriction, (3) treatment of diet containing butterbur, (4) treatment of butterbur combined with diet restriction. Treatment of butterbur combined food restriction significantly (p<0.05) reduced the growth of Bacteroides, Peptococus, Streptococcus, Staphylococcus, and Escherchia coli, respectively. No remarkable changes in the ${\beta}$-glucosidase and ${\beta}$-glucuronidase activities were observed but indole content significanlty decreased. Based on these results, treatment of butterbur combined with diet restriction in rats had a significantly effect for preventing the growth of those pathogenic microorganims.

  • PDF

주요 식이섬유질원이 첨가된 식이가 노화 흰쥐의 장내효소 및 유해산물에 미치는 영향 (Effects of Dietary Fiber on the Bacterial Enzymes and Putrefactive Metabolite in Aged Rats)

  • 강어진;이상선;양차범;신현경
    • 한국식품영양학회지
    • /
    • 제11권5호
    • /
    • pp.488-492
    • /
    • 1998
  • This study was performed to investigate the influcence of various dietary fiber sources in Korea for activities of bacterial enzymes (${\beta}$-glucosidase, ${\beta}$-glucuronidase) and amounts of putrefactive product (indole) in aged rats. ${\beta}$-Glucosidase activity in the intestinal content was significantly lower in the seamustard 15% group than in other groups whereas the activity of ${\beta}$-glucuronidase was higher in the mugwort 15% group than other experimental groups. The amount of indole and pH in the intestinal content of aged rats were significantly lower in mugwort groups than in other groups.

  • PDF

상처처리와 접종시간이 Agrobacterium에 의한 고추 형질전환에 미치는 영향 (Effects of Wounding and Inoculation Time on Agrobacterium -mediated Transformation in Capsicum annuum L.)

  • 전영주;박영두;최근원
    • 원예과학기술지
    • /
    • 제18권6호
    • /
    • pp.797-801
    • /
    • 2000
  • 본 연구는 고추에서 Agrobacterium tumefaciens를 이용한 형질 전환시 형질전환율을 높이기 위하여 A. tumefaciens와의 접종시간과 인위적인 상처처리에 따른 효과를 조사하였던 바 고추 형질전환시 자엽절편체의 재분화는 인위적 상처처리 및 A. tumefaciens 접종시간에 의해 영향을 받았다. 접종시간을 3600초로 한 경우의 재분화는 상처처리에 의해 급격히 감소하였으며 자엽 절편체에 대한 인위적인 상처처리가 20초 또는 120초간의 접종시간처리와 병행됨으로써 형질 전환율을 58-72% 이상으로 높이는 효과를 보였다. 형질전환여부의 확인을 ${\beta}$-glucuronidase 염색법과 npt II primer를 이용한 polymerase chain reaction 분석에 의해 수행 한 바 효과적인 것으로 판단되었다.

  • PDF

Inhibitory Effects of Bifidobacterium spp. Isolated from a Healthy Korean on Harmful Enzymes of Human Intestinal Microflora

  • Park, Hye-Young;Bae, Eun-Ah;Han, Myung-Joo;Choi, Eung-Chil;Kim, Dong-Hyun
    • Archives of Pharmacal Research
    • /
    • 제21권1호
    • /
    • pp.54-61
    • /
    • 1998
  • Five hundreds of bifidobacteria were isolated from a healthy Korean and the inhibitory effects of these isloated bacteria on harmful enzymes of human intestinal microflora were examined by cocultivation of the isolated bifidobacteria with E. coli or total human intestinal microflora. In comparison with the results of E. coli or intestinal microflora cultivation, Bifidobacterium breve K-110, B. breve K-111 and B. infantis K-525 effectively inhibited harmful enzymes ($\beta$-glucuronidase and tryptophanase) of E. coli and lowered the pH of the culture media. Also they inhibited the harmful enzymes ($\beta$-glucosidase, $\beta$-glucuronidase, tryptophanase and urease) and ammonia production of intestinal microflora, and lowered pH of the culture media by increasing lactic acid bacteria of intestinal microflora. When these isolated bifidobacteria were administered on mice, fecal harmful enzymes were also inhibited. Among tested bifidobacteria, B. breve K-110 had the highest inhibitory effect of fecal harmful enzymes.

  • PDF

근채류의 기호도와 장내세균의 유해효소 억제효과 (The Preference and Inhibitory Effect of Root Vegetables on β-Glucuronidase and Tryptophanase of Human Intestinal Bacteria)

  • 한명주;김나영
    • 한국식품조리과학회지
    • /
    • 제15권6호
    • /
    • pp.555-564
    • /
    • 1999
  • 식이섬유를 함유한 근채류의 기호도를 조사하고 장내유산균 증식효과와 장내유해균이 생산하는 ${\beta}$-glucuronidase와 tryptophanase 저해효과를 측정한 결과는 다음과 같다. 1. 조사대상자가 가장 선호하는 근채류는 고구마(4.05)였으며 남자의 선호도는 감자(3.88), 고구마(3.68), 더덕(3.77), 무(3.59), 양파(3.56)의 순으로 나타났으며 여자는 고구마(4.17), 감자(4.03), 양파(3.76), 무(3.61), 더덕(3.55)의 순으로 나타났다. 2. 여자가 남자보다 '근채류가 건강에 좋다'고 인식하고 있었으며 건강에 좋다고 생각하는 근채류는 남자의 경우 마늘 26.4%, 당근 16.7%, 감자 15.3%, 더덕 12.5%의 순으로 나타났고 여자의 경우 마늘 26.7%, 더덕 17.3%, 감자 13.9%, 당근 10.4%의 순으로 나타났다. 3. 근채류 추출물 0.5%를 첨가한 배지에서 장내유익균인 B. breve K-110의 증식효과는 감자(139%), 더덕(145%), 마(164%), 토란(144%), 연근(147%)에서 높았다. 장내유익균(B. breve K-110)과 유해균(E. coli HGU-3와 Bacteroides JY-6)을 혼합배양시 근채류 추출물 0.5% 첨가한 배지에서 장내유해균이 생산하는 유해효소인 ${\beta}$-glucuronidase와 tryptophanase 활성저해 효과는 감자, 더덕, 마, 토란에서 높게 나타났다. 감자, 더덕, 마, 토란추출물을 0.5% 첨가한 배지에 사람의 장내세균총을 배양하였을 때 이들 유해효소의 활성은 50%이상의 억제효과가 나타났다. 4. Sephadex G-100 column chromatography에 의하여 분리한 감자, 마, 더덕, 토란추출물의 고분자 분획에서 장내유익균인 B. breve K-110의 증식효과와 ${\beta}$-glucuronidase와 tryptophanase 활성저해 효과가 높게 나타났다. 5. 분리한 고분자 분획의 ninhydrin 발색반응은 감자, 마, 더덕, 토란에서 음성을 나타내어 아미노산이 아닌 것을 확인하였고 요오드반응은 마, 더덕, 토란에서 음성을 나타내었고 감자에서는 양성을 나타내었다. 이상과 같은 결과에 의하면 근채류중 감자, 마, 더덕, 토란이 유산균을 효과적으로 증식시켜 장내 pH를 저하시킴에 의해 ${\beta}$-glucuronidase, tryptophanase와 같은 장내의 유해효소를 억제할 것이라고 기대되어진다. 그러므로 이들 근채류는 장내의 유해효소에 의해 발생하는 대장암등의 질병예방과 장내균총의 불균형에 의해 야기될 수 있는 설사와 변비같은 질병의 예방에도 효과를 나타낼 것으로 생각된다.

  • PDF