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Optimization for the Process of Ethanol of Persimmon Leaf(Diospyros kaki L. folium) using Response Surface Methodology (반응표면분석법을 이용한 감잎(Diospyros kaki L. folium) 에탄올 추출물의 최적화)

  • Bae, Du-Kyung;Choi, Hee-Jin;Son, Jun-Ho;Park, Mu-Hee;Bae, Jong-Ho;An, Bong-Jeon;Bae, Man-Jong;Choi, Cheong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.218-224
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    • 2000
  • The efforts were made to optimite ethanol extraction from persimmon leaf with the time of extraction$(1.5{\sim}2.5\;hrs)$, the temperature of extraction$(70{\sim}90^{\circ}C)$, and the concentration of ethanol$(0{\sim}40%)$ as three primary variables together with several functional characteristics of persimmon leaf as reaction variables. The conditions of extraction was best fitted by using response surface methodology through the center synthesis plan, and the optimal conditions of extraction were established. The contents of soluble solid and soluble tannin went up as the concentration of ethanol went up and the temperature of extraction went down, and the turbidity went down as the concentration of ethanol went down. Electron donation ability was hardly affected by the extraction temperature and had the tendency to go up as the concentration of ethanol went up. The inhibitory activity of xanthine oxidase(XOase) had the tendency to go up as both the concentration of ethanol and the temperature of extraction went up. The inhibitory activity of angiotensin converting enzyme(ACE), the significance of which still was not recognized, showed the maximum when the concentration of ethanol was 27%. In result, the optimal conditions of extraction was the extraction time of two hours, the extraction temperature of $75{\sim}81^{\circ}C$, and the ethanol concentration of $33{\sim}35%$.

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INFLUENCE OF THREE DIFFERENT PREPARATION DESIGNS ON THE MARGINAL AND INTERNAL GAPS OF CEREC3 CAD/CAM INLAYS (세 가지 다른 인레이 와동 형태가 CEREC3 CAD/CAM의 변연 및 내면 간극에 미치는 영향)

  • Seo, Deog-Gyu;Yi, Young-Ah;Lee, Yoon;Roh, Byoung-Duck
    • Restorative Dentistry and Endodontics
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    • v.34 no.3
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    • pp.177-183
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    • 2009
  • The aim of this study was to evaluate the marginal and internal gaps in CEREC3 CAD/CAM inlays of three different preparation designs. CEREC3 Inlays of three different preparation designs (n=10) were fabricated according to Group I-conventional functional cusp capping/shoulder preparation, Group II-horizontal reduction of cusps and Group III-complete reduction of cusps/shoulder preparation. After cementation of inlays. the bucco-lingual cross section was performed through the center of tooth. Cross section images of 20 magnifications were obtained through the stereomicroscope. The gaps were measured using the Leica application suite software at each reference point. Statistical analysis was performed using one-way ANOVA and Tukey's test (${\alpha}<0.05$). The marginal gaps ranged from 80.0 to $97.8{\mu}m$ for Group I, 42.0 to $194.8{\mu}m$ for Group II, 51.0 to $80.2{\mu}m$ for Group III. The internal gaps ranged from 90.5 to $304.1{\mu}m$ for Group I, 80.0 to $274.8{\mu}m$ for Group II, 79.7 to $296.7{\mu}m$ for Group III. The gaps of each group were the smallest on the margin and the largest on the horizontal wall. For the CEREC3 CAD/CAM inlays, the simplified designs (groups II and III) did not demonstrate superior results compared to the traditional cusp capping design (group I).

Effect of Ethanolic Extract of Schizandra chinensis for the Delayed Ripening Kimchi Preparation (오미자(Schizandra chinensis) 추출물이 김치의 과숙억제에 미치는 영향)

  • Moon, Young-Ja;Park, Sun;Sung, Chang-Keun
    • The Korean Journal of Food And Nutrition
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    • v.16 no.1
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    • pp.7-14
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    • 2003
  • This study mainly focused on to investigate the effects of Schizandra chinensis on the growth of a bacterium, CS6 which was isolated from kimchi. CS6 was final]y identified to lactobacillus plantarum that caused acidification of kimchi. The ethanolic extract of Schizandra chinensis(EES) inhibited the growth of L. plantarum. Minimum inhibition concentration of crude EES on L. plantarum was 62.5mg/$m\ell$. In broth culture, 5$\mu\textrm{g}$/$m\ell$ of EES completely inhibited the growth of L. plantarum during fermentation. The addition of 0.4% of EES has no apparent effect on quality including the taste and color on kimchi. It was expected that EES-containing kimchi could extend the period of preservation. Analysis of organic acids in water fractions of EES was carried out by HPLC. It is apparent that antimicrobial active fractions contained the highest concentration of succinic acid, a little tartaric acid and malic acid. Among these organic acids, succinic acid showed the strong inhibitory effect against L. plantarum CS6 in vitro. Succinic acid-containing kimchi with a concentration of 0.4 and 0.5% had the inhibitory effect on growth of L. plantarum. Inhibitory effect of EES on amylase, cellulase and pectinase was also tested. In conclusion, the present experiment demonstrated that EES inhibited the growth of L. plantarum, and various enzyme activity. EES-containing kimchi was sustained the hardness, and initial acidity during fermentation. EES was considered as the possible additive of kimchi process and EES added in kimchi increase the quality, and storage period of kimchi.

Association between picky eating behaviors and growth in preschool children (유아기 까다로운 식습관과 성장상태 간의 연관성)

  • Shim, Jae Eun;Yoon, Ji Hyun;Kim, Kijoon;Paik, Hee Young
    • Journal of Nutrition and Health
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    • v.46 no.5
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    • pp.418-426
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    • 2013
  • This study was conducted in order to investigate the association between picky eating behaviors of preschool children and growth outcomes. In this study, picky eating behaviors were defined as containing four constructs of 'eating a small amount (ES),' 'neophoic behavior (NB),' 'refusal of specific food groups (RF),' and 'preference for specific food-preparation methods (PP).' A 7-point scale was used for the multi-item questionnaire, which consisted of 21 items (three items for ES, two items for NB, nine items for RF, and seven items for PP), in order to evaluate picky eating behaviors of children. Subjects were recruited among visitors at a medical clinic in Seoul. A total of 150 self-administered survey responses from parents of preschool children were analyzed in order to investigate the association between picky eating behaviors of preschool children and growth outcomes. Height for age (HFA) and weight for height (WFH) z-scores were used for assessment of preschool children's growth. The prevalence of ES, NB, RF, and PP was 44%, 57%, 73%, and 53%, respectively. Children with ES had lower HFA (p < 0.05) and WFH (p < 0.0001) than those without ES, while children with NB, RF, or PP had HFA and WFH were similar to their counterparts. The mean HFA z-score of children with ES was less than 0 (p < 0.05) and the mean WFH z-scores of children with ES, NB, RF, or PP were less than 0 (p < 0.05). According to the study results, related growth outcome differed depending on constructs of picky eating behaviors. In particular, picky eating of ES showed a risk of faltering height growth in preschool children. Further comprehensive studies on the reason for ES and intervention approach is warranted.

Effects of Cornus Officinalis Extract on Blood Fatigue Substance, Muscle Damage and Liver Function during Winter Training in Middle School Male Soccer Players (동계훈련 시 산수유 추출물 섭취가 남중 축구선수의 혈중 피로물질, 근 손상 및 간 기능에 미치는 영향)

  • Park, Sung-Hwan;Ha, Soo-Min;Ha, Min-Seong;Baek, Yeong-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.4
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    • pp.827-838
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    • 2017
  • Twenty male soccer players volunteered as the experimental subjects. The subjects were randomly assigned to the "Winter training with Cornus officinalis extract intake group"(WCI; n=10) and "Winter training group"(WT; n=10). The variables of blood fatigue substance, muscle damage and liver function where measured in all the subjects before the start of the Cornus officinalis intake after at the end of the 4 weeks winter training. Winter training program was proceeded with 6 days a week for 4 weeks. Exercise intensity was measured using the HRR(70~95%). Changes in the heart rate were also measured with the Polar. WCI group were ingested 80 mL of Cornus officinalis extract after every breakfast and dinner for 4 weeks. The test data were analyzed by paired t-test, and independent t-test(%diff), and the alpha level of p<.05 was set for all tests of significance. Lactate in WCI group had significantly decreased, and the changes between the groups were significantly lower in the WCI group than in the WT group. Ammonia in WCI group had significantly increased. CK in WCI group had significantly decreased. LDH in both group had significantly decreased. AST in WCI group had significantly decreased, and the changes between the groups were significantly lower in the WCI group than in the WT group. ALT in WCI group had significantly decreased. In conclusion, the results of this study show that Cornus officinalis extract seem to have a positive influence on blood fatigue substance, muscle damage and liver function. Therefore Cornus officinalis extract is considered to be able to contribute an ergogenic aids.

Relation of polyunsaturated fatty acid, n-3 fatty acid and n-6 fatty acid intakes and atopic dermatitis in the 9~ 11 year old children: KNHANES 2013 ~ 2015 (9 ~ 11세 아동의 불포화지방산, n-3 지방산 및 n-6 지방산의 섭취와 아토피 피부염 과의 관련성 : 2013 ~ 2015년 국민건강영양조사)

  • Kim, Ji-Myung
    • Journal of Nutrition and Health
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    • v.52 no.1
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    • pp.47-57
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    • 2019
  • Purpose: This study was conducted to investigate the relationship between atopic dermatitis and dietary fat and fatty acid (FA) intakes in 9 ~ 11 year old children. Methods: We analyzed data from the combined 2013 ~ 2015 KNHANES (Korean National Health and Nutrition Examination Survey). Subjects were divided into two groups according to atopic dermatitis (AD); with AD and without AD. Data pertaining to macronutrients and FA intakes were obtained by a single 24-h dietary recall. Food sources were identified based on the amounts of total fat and FA consumption according to each food. The associations between each FA intake and atopic dermatitis were analyzed using simple and multiple logistic regression analyses. Age, sex, body mass index (BMI) and income levels were adjusted as covariates. Results: Of the participants, 17.69% suffered from atopic dermatitis. Children with AD had significantly lower fat percentages of total energy and higher carbohydrate percentages of total energy than normal children. Percentages of energy and intakes of polyunsaturated fatty acid (PUFA), n-3 FA and n-6 FA in children with AD were significantly lower than those in normal children. In the FA, linoleic acid, ${\gamma}$-linoleic acid and ${\alpha}$-linolenic acid levels of children with AD were significantly lower than those of normal children. However, the P/S ratio and n-6/n-3 ratio did not differ significantly between children with AD and normal children. Soybean oil was the main contributor to PUFA, n-3 FA and n-6 FA in both groups, while perilla seed oil and mackerel were the major food sources of n-3 FA in children with atopic dermatitis. Atopic dermatitis was significantly correlated with low-fat and high-carbohydrate diets. The adjusted odds ratios were 0.966, 0.776 and 0.963 for PUFA, n-3 FA, and n-6 FA intakes, respectively. Conclusion: The present study provides reliable evidence regarding the relationship between fat and FA intakes and AD in Korean children 9 ~ 11 years of age.

The change of grain quality and starch assimilation of rice under future climate conditions according to RCP 8.5 scenario (RCP 8.5 시나리오에 따른 미래 기후조건에서 벼의 품질 및 전분 동화 특성 변화)

  • Sang, Wan-Gyu;Cho, Hyeoun-Suk;Kim, Jun-Hwan;Shin, Pyong;Baek, Jae-Kyeong;Lee, Yun-Ho;Cho, Jeong-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.4
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    • pp.296-304
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    • 2018
  • The objective of this study was to analyze the impact of climate change on rice yield and quality. Experiments were conducted using SPAR(Soil-Plant-Atmosphere-Research) chambers, which was designed to create virtual future climate conditions, in the National Institute of Crop Science, Jeonju, Korea, in 2016. In the future climate conditions($+2.8^{\circ}C$ temp, 580 ppm $CO_2$) of year 2051~2060 according to RCP 8.5 scenario, elevated temperature and $CO_2$ accelerated the heading date by about five days than the present climate conditions, resulted in a high temperature environment during grain filling stage. Rice yield decreased sharply in the future climate conditions due to the high temperature induced poor ripening. And the spikelet numbers, ripening ratio, and 1000-grain weight of brown rice were significantly decreased compared to control. The rice grain quality was also decreased sharply, especially due to the increased immature grains. In the future climate conditions, expression of starch biosynthesis-related genes such as granule-bound starch synthase(GBSSI, GBSSII, SSIIa, SSIIb, SSIIIa), starch branching enzyme(BEIIb) and ADP-glucose pyrophosphorylase(AGPS1, AGPS2, AGPL2) were repressed in developing seeds, whereas starch degradation related genes such as ${\alpha}-amylase$(Amy1C, Amy3D, Amy3E) were induced. These results suggest that the reduction in yield and quality of rice in the future climate conditions is likely caused mainly by the poor grain filling by high temperature. Therefore, it is suggested to develop tolerant cultivars to high temperature during grain filling period and a new cropping system in order to ensure a high quality of rice in the future climate conditions.

Multi-Category Sentiment Analysis for Social Opinion Related to Artificial Intelligence on Social Media (소셜 미디어 상에서의 인공지능 관련 사회적 여론에 대한 다 범주 감성 분석)

  • Lee, Sang Won;Choi, Chang Wook;Kim, Dong Sung;Yeo, Woon Young;Kim, Jong Woo
    • Journal of Intelligence and Information Systems
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    • v.24 no.4
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    • pp.51-66
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    • 2018
  • As AI (Artificial Intelligence) technologies have been swiftly evolved, a lot of products and services are under development in various fields for better users' experience. On this technology advance, negative effects of AI technologies also have been discussed actively while there exists positive expectation on them at the same time. For instance, many social issues such as trolley dilemma and system security issues are being debated, whereas autonomous vehicles based on artificial intelligence have had attention in terms of stability increase. Therefore, it needs to check and analyse major social issues on artificial intelligence for their development and societal acceptance. In this paper, multi-categorical sentiment analysis is conducted over online public opinion on artificial intelligence after identifying the trending topics related to artificial intelligence for two years from January 2016 to December 2017, which include the event, match between Lee Sedol and AlphaGo. Using the largest web portal in South Korea, online news, news headlines and news comments were crawled. Considering the importance of trending topics, online public opinion was analysed into seven multiple sentimental categories comprised of anger, dislike, fear, happiness, neutrality, sadness, and surprise by topics, not only two simple positive or negative sentiment. As a result, it was found that the top sentiment is "happiness" in most events and yet sentiments on each keyword are different. In addition, when the research period was divided into four periods, the first half of 2016, the second half of the year, the first half of 2017, and the second half of the year, it is confirmed that the sentiment of 'anger' decreases as goes by time. Based on the results of this analysis, it is possible to grasp various topics and trends currently discussed on artificial intelligence, and it can be used to prepare countermeasures. We hope that we can improve to measure public opinion more precisely in the future by integrating empathy level of news comments.

Exploratory Study of Person Centered Care Practice in Korean Long-term Care Facilities using DCM(Dementia Care Mapping) as a tool (DCM(Dementia Care Mapping)을 활용한 한국 요양시설에서의 사람중심케어 실천의 탐색적 연구)

  • Kim, Dongseon
    • 한국노년학
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    • v.41 no.2
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    • pp.197-215
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    • 2021
  • This study aims to evaluate Person Centered Care practice and characteristics of care services in Korean long-term care facilities using Dementia Care Mapping as a tool. DCM, systematic observational evaluation tool for measuring dementia patients' QOL, was transformed into self-report rating scale. The process of transforming DCM into a scale of 34 items involves operationalization of DCM concepts and it's adaptation into Korean long-term care practices. Review by research team of Bradford university was added to maintain DCM concept and meaning in this scale. The scale with Cronbach alpha of .88 was surveyed on 343 care workers. Survey result shows PCC value practiced by them is 3.77(of 5 likert scale) and values on each categories of PCC reveal the characteristics of care in Korean facilities; attachment(4.02), comfort(3.95), inclusion(3.89), identity(3.67) and occupation(3.41). Dementia care in Korean facilities focuses on recipients'safety, comfort but lacks individualistic care and the meaningful and fulfilling occupation for patients. Looking at the organizational and individual factors influencing DCM values, the small facilities showed higher PCC values and there are no significant difference in PCC values between public and private facilities. Managers and care workers with career of 1~2 years showed higher PCC values compared to other career ranks and lengthes. This study suggests care practice should be centered on personhood of patients in long-term care facilities, for which introduction of unit care and education of PCC for service providers including support personnel are needed. DCM and Korean DCM scale developed in this study are suggested for the PCC-based assessment on care quality.

Comparative Analysis of Diversity Characteristics (γ-, α-, and β-diversity) of Biological Communities in the Korean Peninsula Estuaries (하구 순환 유지 여부에 따른 하구 주요 생물 군집별 다양성 특성 연구: 열린하구와 닫힌하구에서의 γ-, α- 및 β-다양성 비교)

  • Oh, Hye-Ji;Jang, Min-Ho;Kim, Jeong-Hui;Kim, Yong-Jae;Lim, Sung-Ho;Won, Doo-Hee;Moon, Jeong-Suk;Kwon, Soonhyun;Chang, Kwang-Hyeon
    • Korean Journal of Ecology and Environment
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    • v.55 no.1
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    • pp.84-98
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    • 2022
  • Estuary is important in terms of biodiversity because it has the characteristics of transition waters, created by the mixing of fresh- and seawater. The estuarine water circulation provides a variety of habitats with different environments by inducing gradients in the chemical and physical environment, such as water quality and river bed structure, which are ultimately the main factors influencing biological community composition. If the water circulation is interrupted, the loss of brackish areas and the interception of migration of biological communities will lead to changes in the spatial distribution of biodiversity. In this study, among the sites covered by the Estuary Aquatic Ecosystem Health Assessment, we selected study sites where changes in biodiversity can be assessed by spatial gradient from the upper reaches of the river to the lower estuarine area. The α-, γ- and β-diversity of diatom, benthic macroinvertebrates, and fish communities were calculated, and they were divided into open and closed estuary data and compared to determine the trends in biodiversity variation due to estuarine circulation. As results, all communities showed higher γ-diversity at open estuary sites. The benthic macroinvertebrate community showed a clear difference between open and closed estuaries in β-diversity, consequently the estuarine transects were considered as a factor that decreases spatial heterogeneity of their diversity among sites. The biodiversity trends analyzed in this study will be used to identify estuaries with low γ- and β-diversity by community, providing a useful resource for further mornitoring and management to maintain estuarine health.