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http://dx.doi.org/10.14405/kjvr.2020.60.3.173

Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal  

Son, Se Hyun (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Seo, Kwang Won (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Kim, Yeong Bin (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Noh, Eun Bi (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Lee, Keun-Woo (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Oh, Tae-Ho (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Kim, Seung-Joon (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Song, Jae-Chan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Kim, Tae-Wan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Lee, Young Ju (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Research / v.60, no.3, 2020 , pp. 173-177 More about this Journal
Abstract
Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.
Keywords
Escherichia coli; edible offal; fluoroquinolones; quinolone resistance-determining region; molecular typing;
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