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http://dx.doi.org/10.12989/mwt.2018.9.3.195

Effects of N-acetylcysteine on biofilm formation by MBR sludge  

Song, WonJung (Department of Environmental Engineering, Konkuk University)
Lade, Harshad (Department of Environmental Engineering, Konkuk University)
Yu, YoungJae (Department of Environmental Engineering, Konkuk University)
Kweon, JiHyang (Department of Environmental Engineering, Konkuk University)
Publication Information
Membrane and Water Treatment / v.9, no.3, 2018 , pp. 195-203 More about this Journal
Abstract
N-acetylcysteine (NAC) has been widely used as an initial mucolytic agent and is generally used as an antioxidant to help alleviate various inflammatory symptoms. NAC reduces bacterial extracellular polymeric substances (EPS) production, bacterial adhesion to the surface and strength of mature biofilm. The efficacy has been shown to inhibit proliferation of gram-positive and gram-negative bacteria. In membrane bioreactor (MBR) processes, which contain a variety of gram negative bacteria, biofilm formation has become a serious problem in stable operation. In this study, use of NAC as an inhibitor of biofilm contamination was investigated using the center for disease control (CDC) reactors with MBR sludge. Biomass reduction was confirmed with CLSM images of membrane surfaces by addition of NAC, which was more efficient as the concentration of NAC was increased to 1.5 mg/mL. NAC addition also showed decreases in EPS concentrations of the preformed biofilm, indicating that NAC was able to degrade EPS in the mature biofilm. NAC addition was also effective to inhibit biofilm formation by MBR sludge, which consisted of various microorganisms in consortia.
Keywords
Extracellular polymeric substance; N-acetyl cysteine; MBR sludge; CDC reactor; biofilm formation;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Al-Halbouni, D., Traber, J., Lyko, S., Wintgens, T., Melin, T., Tacke, D., Janot, A., Dott, W. and Hollender, J. (2008), "Correlation of EPS content in activated sludge at different sludge retention times with membrane fouling phenomena", Water Res., 42(6), 1475-1488.   DOI
2 Domenech, M. and Garcia, E., (2017) "N-Acetyl-L-Cysteine and cysteamine as new strategies against mixed biofilms of nonencapsulated streptococcus pneumoniae and nontypeable haemophilus influenza", Antimicrobial Agent. Chemotherapy, 61(2) 1-10
3 Gogate, P.R. (2007), "Application of cavitational reactors for water disinfection: Current status and path forward", J. Environ. Manage., 85(4), 801-815.   DOI
4 Kappachery, S., Paul, D. and Kweon, J.H. (2012), "Effect of Nacetylcysteine against biofouling of reverse osmosis membrane", Desalination, 285, 184-187.   DOI
5 Lade, H., Paul, D. and Kweon, J.H. (2015), "Combined effects of curcumin and (-)-epigallocatechin gallate on inhibition of NAcylhomoserine lactone-mediated biofilm formation in wastewater bacteria from membrane bioreactor", J. Microbiol. Biotechnol, 25(11), 1908-1919.   DOI
6 Nam, A., Kweon, J., Ryu, J., Lade, H. and Lee, C. (2015), "Reduction of biofouling using vanillin as a quorum sensing inhibitory agent in membrane bioreactors for wastewater treatment", Membr. Water Treat., 6(3), 189-203.   DOI
7 Marchese, A., Bozzolasco, M., Gualco, L., Debbia, E.A., Schito, G.C. and Schito, A.M. (2003), "Effect of fosfomycin alone and in combination with N-acetylcysteine on E. coli biofilms", Int. J. Antimicrobial Agents, 22, 95-100.   DOI
8 Masse, A., Sperandio, M. and Cabassud, C. (2006), "Comparison of sludge characteristics and performance of a submerged membrane bioreactor and an activated sludge process at high solids retention time", Water Res., 40(12), 2405-2415.   DOI
9 Muranaka, L.S., Giorgiano, T.E., Takita, M.A., Forim, M.R., Silva, L.F., Coletta-Filho, H.D., Machado, M.A., and de Souza, A.A. (2013), "N-Acetylcysteine in agriculture, a novel use for an old molecule: Focus on controlling the plant-pathogen xylella fastidiosa", PloS one, 8(8), e72937.   DOI
10 Oh, H.S., Kim, S.R., Cheong, W.S., Lee, C.H., and Lee, J.K. (2013), "Biofouling inhibition in MBR by Rhodococcus sp. BH4 isolated from real MBR plant", Appl.Microbiol. Biotechnol., 97(23), 10223-10231.   DOI
11 Olofsson, A.C., Hermansson, M. and Elwing, H. (2003), "Nacetyl-L-cysteine affects growth, extracellular polysaccharide production, and bacterial biofilm formation on solid surfaces", Appl. Environ. Microbiol., 69(8), 4814-4822.   DOI
12 Parry, M.F. and Neu, H.C. (1977), "Effect of N-acetylcysteine on antibiotic activity and bacterial growth in vitro", J. Clinical Microbiol., 5(1), 58-61.
13 Saad, M.A. (1992), "Biofouling prevention in RO polymeric membrane systems", Desalination, 88(1-3), 85-105.   DOI
14 Perez-Giraldo, C., Rodriguez-Benito, A., Moran, F.J., Hurtado, C., Blanco, M.T. and Gomez-Garcia, A.C. (1997), "Influence of Nacetylcysteine on the formation of biofilm by Staphylococcus epidermidis", J. Antimicrobial Chemotherapy, 39(5), 643-646.   DOI
15 Quah, S.Y., Wu, S., Lui, J.N., Sum, C.P. and Tan, K.S. (2012), "N-acetylcysteine inhibits growth and eradicates biofilm of Enterococcus faecalis", J. Endodontics, 38(1), 81-85.   DOI
16 Roberts, D. and Cole, P. (1981), "N-acetylcysteine potentiates the anti-pseudomonas activity of carbenicillin in vitro", J. Infection, 3(4), 353-359.   DOI
17 Silveira, L.F. M., Baca, P., Arias-Moliz, M.T., Rodriguez-Archilla, A. and Ferrer-Luque, C.M. (2013), "Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against Enterococcus faecalis biofilm", J. Oral Sci., 5(3), 146-149.   DOI
18 Smith, P.J., Vigneswaran, S., Ngo, H.H., Ben-Aim, R. and Nguyen, H. (2006), "A new approach to backwash initiation in membrane systems", J. Membr. Sci., 278(1), 381-389.   DOI
19 Stey, C., Steurer, J., Bachmann, S., Medici, T. C. and Tramer, M.R. (2000), "The effect of oral N-acetylcysteine in chronic bronchitis: a quantitative systematic review", Euro. Respir. J., 16(2), 253-262.   DOI
20 Tsuneda, S., Aikawa, H., Hayashi, H., Yuasa, A. and Hirata, A. (2003), "Extracellular polymeric substances responsible for bacterial adhesion onto solid surface", FEMS Microbiol. Letters, 223(2), 287-292.   DOI
21 Vargas, A., Moreno-Andrade, I. and Buitron, G. (2008), "Controlled backwashing in a membrane sequencing batch reactor used for toxic wastewater treatment", J. Membr. Sci., 320(1), 185-190.   DOI
22 Zhao, T., and Liu, Y. (2010), "N-acetylcysteine inhibit biofilms produced by Pseudomonas aeruginosa", BMC Microbiol., 10(1), 140.   DOI
23 Wang, G., Huang, L. and Xia, Z. (2016), "Study of membrane fouling properties of landfill leachate treatment by the SBACMBR process", Environ. Eng. Sci., 33(12), 978-985.   DOI
24 Wang, Z., Wu, Z. and Tang, S. (2009), "Extracellular polymeric substances (EPS) properties and their effects on membrane fouling in a submerged membrane bioreactor", Water Res., 43(9), 2504-2512.   DOI
25 Zeng, J., Gao, J.M., Chen, Y.P., Yan, P., Dong, Y., Shen, Y., Gue, J.S., Zeng, N. and Zhang, P. (2016), "Composition and aggregation of extracellular polymeric substances (EPS) in hyperhaline and municipal wastewater treatment plants", Scientific Reports, 6.