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http://dx.doi.org/10.3746/jkfn.2010.39.11.1705

Analyses of Capsaicinoids and Ascorbic Acid in Pepper (Capsicum annum L.) Breeding Lines  

Jung, Mi-Ri (Dept. of Food Science and Technology, Chungbuk National University)
Hwang, Young (Korean Food & Culture Division, Rural Development Administration)
Kim, Hae-Young (Dept. of Food Science and Nutrition, Yong In University)
Jeong, Heong-Sang (Dept. of Food Science and Technology, Chungbuk National University)
Park, Ji-Sung (Jeil Seed Company)
Park, Dong-Bok (Jeil Seed Company)
Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.39, no.11, 2010 , pp. 1705-1709 More about this Journal
Abstract
Peppers (Capsicum annuum L.) are a rich source of phytochemicals including polyphenolics, flavonoids, capsaicinoids and ascorbic acid. Capsaicinoids are a group of 12 or more related alkaloids responsible for the pungent sensation in the fruits of the genus Capsicum. Ascorbic acid is another functional and nutritional constituent of peppers. In this study, the contents of two major capsaicinoids (capsaicin and dihydrocapsaicin) and ascorbic acid in 131 pepper breeding lines were quantified by HPLC. In 131 pepper breeding lines, capsaicin and dihydrocapsaicin contents were in the range of 0.0 to 219.6 and 0.0 to 110.8 mg/100 g, respectively. The breeding lines with higher capsaicin content contained higher dihydrocapsaicin content as well. Ascorbic acid contents were 264.9 to 1695.5 mg/100 g for the 131 pepper breeding lines. The analytical method validation parameters including accuracy, repeatability, and reproducibility were calculated to ensure the method's validity. This study provides basic information to plant breeders and biotechnologists who are planning to breed genotypes with high content of phytochemicals.
Keywords
pepper; capsaicinoids; ascorbic acid; HPLC; breeding lines;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By SCOPUS : 6
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1 Cho YS, Cho MC, Suh HD. 2000. Current status and projects of national hot pepper industry in Korea. J Korean Capsicum Res Coop 6: 1-27.
2 Park JB, Lee SM, Kim S. 2000. Capsaicinoids control of red pepper powder by particle size. J Korean Capsicum Res Coop 6: 51-62.
3 Kang IH. 1983. Hankook Shiksenghwalsa. Samyongsa, Seoul, Korea. p 190.
4 Park CR, Lee KJ. 1975. A study on the influence of drying methods upon the chemical change in red pepper, part 2, changes of free amino acid, free sugar. Korean J Nutr 8: 27-31.   과학기술학회마을
5 Kim S, Kim KS, Park JB. 2006. Changes of various chemical components by the difference of the degree of ripening and harvesting factors in two single-harvested peppers (Capsicum annuum L.). Korean J Food Sci Technol 38: 615-620.   과학기술학회마을
6 Lee SW. 1979. Gas liquid chromatographic studies on sugars and organic acid in different portions of hot pepper fruit (Capsicum annuum L.). Korean J Food Sci Technol 11: 278-282.   과학기술학회마을
7 Kawaguci Y, Ochi T, Takaishi Y, Kawazoe K, Lee KH. 2004. New sesquiterpenes from Capsicum annuum L. J Nat Prod 67: 1893-1896.   DOI
8 Wang HF, Tsai YS, Lin ML, Ou AS. 2006. Comparison of bioactive components in GABA tea and green tea produced in Taiwan. Food Chem 96: 648-653.   DOI
9 Shin HH, Lee SR. 1991. Quality attributes of Korean red pepper according to cultivars and growing areas. Korean J Food Sci Technol 23: 296-300.   과학기술학회마을
10 Kim S, Park JH, Wang IK. 2002. Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. J Food Sci 67: 2957-2961.   DOI
11 Park JS, Kim MH, Yu RN. 1999. Approximative study of nutrients and intakes determined from capsaicin contents in powdered soups of Korean instant noodles and hot pepper. J Korean Soc Food Sci Nutr 28: 501-504.   과학기술학회마을
12 Choi SH. 2006. Ascorbic acid of Korean pepper by cultivating season, region and cooking method. J East Asian Soc Dietary Life 16: 578-584.   과학기술학회마을
13 Chai JY, Kim MS, Han IK, Lee SY, Yeo IH. 1994. Relationships between the content and sensory evaluation of pungent principles in red pepper. J Korean Soc Anal Sci 7: 541-545.   과학기술학회마을
14 Kye SH, Lee JD, Paik HY. 1993. Analysis of ascorbic acid contents in raw, processed and cooked foods by HPLC. J Korean Home Assoc 31: 201-208.   과학기술학회마을
15 Cho BC, Park KW, Kang HM, Lee WM, Choe JS. 2004. Correlationship between climatic elements and internal characteristics of red pepper fruit in different growing periods. J Bio-Env Con 13: 67-72.   과학기술학회마을
16 Davis BH, Mathews S, Kirk JTO. 1970. The nature and biosynthesis of the carotenoids of different colour varieties of Capsicum annuum L. Phytochemistry 9: 797-800.   DOI
17 Materska M, Piacente S, Stochmal A, Pizza C, Olezek W, Peruka I. 2003. Isolation and structure elucidation of flavonoid and phenolic acid glycosides from pericarp of hot pepper fruit Capsicum annuum L. Phytochemistry 63: 893-898.   DOI
18 Chiang GH. 1986. HPLC analysis of capsaicins and simultaneous determination of capsaicins and piperine by HPLC-ECD and UV. J Food Sci 51: 499-505.   DOI
19 Zhang WY, Po ALW. 1994. The effectiveness of topically applied capsaicin. Eur J Clin Pharmacol 46: 517-522.
20 Mori A, Lehman S, O'kelly J, Kumagai T, Desmond JC, Pervan M, McBride WH, Kizaki M, Koeffler P. 2006. Capsaicin, a component of red pepper, inhibits the growth of androgen-independent, p53 mutant prostate cancer cells. Cancer Res 66: 3222-3229.   DOI
21 Howard LR, Smith RT, Wagner AB, Villalon B, Burns EE, 1994. Provitamin A and ascorbic acid content of fresh pepper cultivars (Capsicum annuum L.) and processed jalapenos. J Food Sci 59: 362-365.   DOI
22 Topuz A, Ozdemir F. 2007. Assessment of carotenoids, capsaicinoids and ascorbic composition of some selected pepper cultivars (Capsicum annuum L.) grown in Turkey. J Food Compos Anal 20: 596-602.   DOI
23 Deepa N, Kaur C, George B, Singh B, Kapoor HC. 2007. Antioxidant constituents in some sweet pepper (Capsicum annuum L.) genotypes during maturity. Food Sci Technol 40: 121-129.
24 Attuquayefio VK, Buckle KA. 1987. Rapid sample preparation method for HPLC analysis of capsaicinoids in capsicum fruits and oleoresins. J Agric Food Chem 35: 777-779.   DOI