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http://dx.doi.org/10.3746/jkfn.2004.33.7.1186

Metabolism Activity of Bifidobacterium spp. by D.Ps of Konjac Glucomannan Hydrolysates  

최준영 (경원대학교 생명공학부 분자ㆍ식품생명공학전공)
박귀근 (경원대학교 생명공학부 분자ㆍ식품생명공학전공)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.7, 2004 , pp. 1186-1191 More about this Journal
Abstract
Bacillus sp. $\beta$-mannanase was purified by DEAE-sephadex ion exchange column chromatography. The partially purified P-mannanase exhibited maximum activity at pH 6.0 and 5$0^{\circ}C$, and was stable at a pH range of 5.5 to 7.0, and at temperature between 30 to 5$0^{\circ}C$. Konjac glucomannan was hydrolyzed by the purified $\beta$-mannanase, and then hydrolysates separated by 1st activated carbon column chromatography and 2nd sephadex G-25 gel filtration. The main hydrolysates were composed of D.P 5 and 7 glucomannooligosaccharides by TLC and FACE method. To investigate the effects of guar gum glucomannooligosaccharides on the in vitro growth of B. longum, B. bifidum, B. infantis, B. adolescentis, B. animalis, and B. breve, Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon SOUTce such as D.P 5, and D.P 7 glucomannooligosaccharides, respectively. B. longum grew up 4.6-fold and 5.3-fold more effectively by the replacement of D.P 5 and 7 glucomannooligosaccharides as the carbon source in a comparasion of standard MRS. Also, B. breve and B. animalis slightly grew up by the treatment of D.P 5 glucomannooligosaccharide.
Keywords
P-mannanase; Bifidobacterium spp; konjac glucomannan;
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1 Hoffmann K, Bircher J. 1969. Ver nderungen der bakteriellen Darmbesiedlung nach Lactulose-gaben. Schweiz Med Wschr 99: 608-613.
2 Gyrgy P, Norris RF, Rose CS. 1954. Bifidus factor. I. A variant of Lactobacillus bifidus requiring a special growth factor. Arch Biochem Biophys 4: 193-198.
3 Haenel H, Bending J. 1975. Growth effect of branched oligosaccharides on principal intestinal bacteria. In Progress in food and nutrition science. Pergamon Press, Oxford, New York. Vol 1, p 21-27.
4 Kusakabe I, Takahashi R, Kusama S, Murakami K, Maekawa A, Suzuki T. 1985. Struture of the glucomannooligosaccharides resulting from the hydrolysis of konjac glucomannan produced by a $\beta$-mannanase from Streptomyces sp. Agic Biol Chem 48: 2943-2950.
5 Moon JW, Choi SH, Shin YK, Lee SW, Kang KH. 1988. Effect on the growth of Bifidobacterium spp. by transgalactooligosaccharides produced with $\beta$-galactosidase. Korean J Dairy Sci 20: 283-289.
6 Lowry OH, Rosebrough NJ, Fan AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-271.
7 Miller GL. 1959. Use of dinitrosalicylic acid regent for determination of reducing sugar. Anal Chem 31: 426-428.   DOI
8 Jackson P. 1996. Carbohydrate electrophoresis methods by the induced ANTs. Mol Biotechnol 5: 101-123.   DOI   ScienceOn
9 Choi JY, Park GG. 2004. Purification of Bacillus sp. $\beta$-mannanase and the metabolism activity of Bifidobacterium spp. by D.Ps of guar gum hydrolysates. Kor J Microbiol Biotechnol (in press).
10 McCleary BV. 1982. Purification and properties of a mannoside mannohydrolase from guar. Carbohydr Res 101: 74-92.
11 Kim WD, Kobayashi O, Kaneko S, Sakakibara Y, Park GG, Kusakabe I, Tanaka H, Kobayashi H. 2002. $\alpha$-Galactosidase from cultured (Oryza sativa L. var. Nipponbare) cells. Phytochemistry 61: 621-630.   DOI   ScienceOn
12 Mitsuoka T. 1982. Recent trends in research on intestinal flora. Bifidobacteria Microflora 1: 3-11.   DOI
13 Kobayashi Y, Echizen R, Mutai M. 1984. Intestinal flora and dietary factors. Processings of the 4th RIKEN symposium on intestinal flora. Japan Scientific Societies press, Tokyo. p 69.
14 Ohtsuka KY, Benno YK, Endo H, Ueda OT, Mitsuoka T, Kobayashi H. 1989. Effect of 4'-galactosyllactose intake on human fecal microflora. Bifidus 2: 143-149.
15 Akino T, Nakamura N, Horikoshi K. 1987. Poduction of $\beta$-mannosidase and $\beta$-mannanase by an alkalophilic Bacillus sp. Appl Microbiol Biotechnol 26: 323-327.
16 Akino T, Nakamura W, Horikoshi K. 1998. Chariacterization of three $\beta$-mannanase by an alkalophilic Bacillus sp. Agric Biol Chem 52: 773-779.
17 Marga F, Ghakis C, Duport C, Morosoli R, Kluefel D. 1996. Improved production of mannanase by Streptomyces lividans. Appl Environ Microbiol 62: 4656-4658.
18 Mitsuoka T. 1990. Bifidobacteria and their role in human health. J Ind Microbol 6: 263-269.   DOI
19 Min DS, Chung YJ, Bai DH, Yu JH. 1995. Production of $\beta$-mannanase by an akali-tolerant Bacillus sp. YA-14. Foods Biothchnol 4: 285-292.