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http://dx.doi.org/10.13103/JFHS.2016.31.1.51

Induction of Apoptosis in Human Cancer Cells with Extracts of Taraxacum coreanum, Youngia sonchifolia and Ixeris dentate  

Shin, Seong-Ah (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Hae-Nim (Department of Companion and Laboratory Animal Science, Kongju National University)
Choo, Gang-Sik (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Hyeong-Jin (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.1, 2016 , pp. 51-58 More about this Journal
Abstract
This research examined the effects of Compositae extract on the inhibition of proliferation and apoptosis in human breast and human gastric cancer cells. Compositae extracts which is used in the experiment are Taraxacum coreanum (TC), Youngia sonchifolia (YS) and Ixeris dentata (ID). The proliferation of SK-BR-3, MDA-MB-231 and AGS cells were investigated by MTT assay. ID and YS extracts inhibited proliferation of SK-BR-3, MDA-MB-231 and AGS cells in a dose-dependent manner, but TC have barely affected. In addition, the most effective extract was ID. To assess the apoptosis of ID extract, the nuclei of human cancer cells were stained with DAPI solution respectively. Chromatin condensation, indicated apoptosis, was increased in a dose-dependent manner. We investigated change of ID extract-induced apoptosis proteins on human cancer cells by western blot analysis. The level of Bcl-2 decreased, whereas the level of Bax, cleaved-PARP increased in dose-dependent manner compared with non-treatment. Also Bax/Bcl-2 ratio, which is used in clinical indicator of apoptosis, was increased at ID extract treatment group compared with non-treatment. Moreover the Bax/Bcl-2 ratio of MDA-MB-231 cell was significantly increased as against SK-BR-3, AGS cells. These results indicated that ID extract have anti-proliferation effect better than YS or TC, and induced apoptosis in human breast cancer MDA-MB-231 cell better than human breast cancer SK-BR-3 cell, human gastric cancer. Even if further research is needed, ID can be developed as a chemopreventive or therapeutic agent of breast cancer.
Keywords
Taraxacum coreanum; Youngia sonchifolia; Ixeris dentata; apoptosis; anticancer;
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