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Enhancement of Immunomodulatory Activities of Low Molecular Weight Fucoidan Isolated from Hizikia fusiforme  

Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University)
Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National University)
Han, Jae-Gun (College of Bioscience and Biotechnology, Kangwon National University)
Jin, Ling (College of Bioscience and Biotechnology, Kangwon National University)
Jeong, Hyang Suk (College of Bioscience and Biotechnology, Kangwon National University)
Choi, Geun-Pyo (Department of Food Processing and Bakery, Gangwon Provincial College)
Park, Uk-Yeon (Department of Food Processing and Bakery, Gangwon Provincial College)
You, Sang-Guan (Faculty of Marine Bioscience and Technology, Kangnung National University)
Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Korean Journal of Food Science and Technology / v.40, no.5, 2008 , pp. 545-550 More about this Journal
Abstract
The anticancer and immunomodulatory activities of low molecular weight (Mw 11 kDa) fucoidan isolated from Hizikia fusiforme (H. fusiforme) via the ultrasonification extraction process were assessed in this study. Low molecular weight fucoidan improved the growth of human B and T cells, up to approximately 40% as compared to the controls (untreated) and 30% for commercially available fucoidan (Mw 150 kDa). IL-6 and TNF-$\alpha$ were secreted from human B cells at levels of $7.8\times10^{-4}$ pg/mL and $7.2\times10^{-4}$ pg/mL, respectively, and these levels were higher than the levels measured in the controls and with other high molecular weight fucoidan. It was also determined that the cytokine from human B and T cells cultivated with added fucoidan enhanced the growth of human NK cells. The fucoidan isolated from H. fusiforme showed low cytotoxicity, approximately 19%, after the addition of 1.0 mg/mL, the highest tested concentration. The growth of human lung cancer cells (A549) and human breast cancer cells (MCF-7) were inhibited by 69.8% and 83.3%, respectively. These results demonstrated that the low molecular weight fucoidan isolated from H. fusiforme has potential as a new functional food component that evidences immunomodulatory activities and anticancer activity. One of the primary positive features of this fucoidan is that low molecular weight polysaccharides can be readily handled during processing.
Keywords
Hizikia fusiforme; low molecular weight fucoidan; immune activities;
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