Effect of Blood Contamination on the Push-Out Bond Strength and Surface Morphology of Tricalcium Silicate Materials

The aim of this study was to evaluate the effect of blood contamination on the push-out bond strength and surface morphology of tricalcium silicate materials; Biodentine$^{(R)}$, Theracal$^{(R)}$ and mineral trioxide aggregate. The standardized lumens of root slices prepared from extracted single-root human teeth were filled with Biodentine$^{(R)}$, Theracal$^{(R)}$ and mineral trioxide aggregate by manufacturer's instruction. The specimens were randomly divided into 2 groups (n = 20) for each material and then incubated for 4 days at $37^{\circ}C$; control group (phosphate buffered saline solution) and experimental group (fetal bovine serum). The push-out bond strengths were then measured by a universal testing machine and the surface morphology of each experimental group was analyzed by scanning electron microscope. Biodentine$^{(R)}$ and Theracal$^{(R)}$ showed higher push-out bond strength compared with mineral trioxide aggregate after exposure to fetal bovine serum. A substantial change in the surface morphology of each material was observed after exposure to fetal bovine serum. In conclusion, the push-out bond strengths of Biodentine$^{(R)}$ and Theracal$^{(R)}$ were higher than mineral trioxide aggregate when exposed to blood contamination. Therefore, it is supposed that the use of Biodentine$^{(R)}$ and Theracal$^{(R)}$ is appropriate in the presence of blood.

혈액오염이 Tricalcium Silicate 재료의 압출강도와 표면형태에 미치는 영향

이 연구의 목적은 혈액오염 시 Biodentine$^{(R)}$, Theracal$^{(R)}$, mineral trioxide aggregate(MTA)의 압출강도를 측정하고 표면형태를 관찰하는 것이었다. Biodentine$^{(R)}$, Theracal$^{(R)}$, MTA 각각의 재료를 2개의 그룹으로 나누었다. 대조군은 phosphate buffered saline 용액 조건에서, 실험군은 fetal bovine serum 조건에서 4일간 $37^{\circ}C$에서 보관하였다. 이후 압출강도를 측정하고 주사전자현미경을 이용하여 표면형태를 분석하였다. Biodentine$^{(R)}$과 Theracal$^{(R)}$은 모든 조건에서 MTA보다 유의하게 더 높은 압출강도를 보였고 혈액오염 시 모든 재료의 압출강도는 유의하게 감소하였다. 표면형태 관찰결과 혈액오염 후 모든 재료의 표면형태가 변화하였다. 혈액오염 조건에서 Biodentine$^{(R)}$과 Theracal$^{(R)}$은 MTA와 비교하여 더 높은 압출강도를 보였으므로 혈액오염 조건에 사용하기에 더 적절할 수 있다.