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http://dx.doi.org/10.5010/JPB.2018.45.2.131

Effect of carbon source and concentration on in vitro regeneration and propagation in Lycopus lucidus by node culture  

Lee, Na-Nyum (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Kim, Ji Ah (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Kim, Yong Wook (Special Forest Products Division, National Institute of Forest Science (NIFoS))
Kim, Tae Dong (Forest Biotechnology Division, National Institute of Forest Science (NIFoS))
Publication Information
Journal of Plant Biotechnology / v.45, no.2, 2018 , pp. 131-139 More about this Journal
Abstract
We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.
Keywords
Lycopus lucidus Turcz. ex Benth.; Carbon source; Regeneration; Acclimatization;
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1 Cui HZ, Oh HC, Li X, Lee YJ, Cho KW, Kang DG, Lee HS (2013) Ethanol extract of Lycopus lucidus elicits positive inotropic effect via activation of $Ca^{2+}$ entry and $Ca^{2+}$ release in beating rabbit atria. J Med Food 16:633-640   DOI
2 Donnelly DJ, Vidaver WE (1984) Leaf anatomy of red raspberry transferred from culture to soil. J Amer Soc Hort Sci 109:177-181
3 Hazarika B N (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704-1712
4 Hussain MS, Fareed S, Ansari S, Rahman MA, Ahmad IZ, and Saeed M (2012) Current approaches toward production of secondary plant metabolites. J Pharm Bioallied Sci 4:10-20
5 Izabela GK, Kuzma Ł, Wysokinska H (2015) The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpine. Plant Cell Tissue Organ Cult 122:699-708   DOI
6 Jaime A, Silva T (2004) The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers. Bragantia 63(2):165-177   DOI
7 Kebrom TH, Mullet JE (2016) Transcriptome profiling of tiller buds provides new insights into PhyB regulation of tillering and indeterminate growth in sorghum. Plant Physiol 170:2232-2250   DOI
8 Kim JA, Moon HK, Choi YE (2013) Microtuber formation from in vitro Codonopsis lanceolata plantlets by sugar. J Plant Biotechnol 40:147-155   DOI
9 Koch K (1996) Carbohydrate-modulated gene expression in plants. Annu Rev Plant Biol 47:509-540   DOI
10 Lee YJ, Kang DG, Kim JS, Lee HS (2008) Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol 48:38-46   DOI
11 Mason MG, Ross JJ, Babst BA, Wienclaw BN, Beveridgea CA (2014) Sugar demand, not auxin, is the initial regulator of apical dominance. PNAS 111:6092-6097   DOI
12 Lin CR, Zuo SY, Xiong W, Chen GY (2012) Antioxidation effects of Lycopus lucidus polyscharides on aged mice induced by D-Galactose. Chinese Pharmacolol Bull 29:1440-1443
13 Lu YH, Huang JH, Li YC, Ma TT, Sang P, Wang WJ, Gao CY (2015) Variation in nutritional compositions, antioxidant activity and microstructure of Lycopus lucidus Turcz. root at different harvest times. Food Chem 183:91-100   DOI
14 MacGregor DR, Deak KI, Ingram PA, Malamy JE (2008) Root system architecture in Arabidopsis grown in culture is regulated by sucrose uptake in the aerial tissues. Plant Cell 20:2643-2660   DOI
15 Thomas BR, Rodriguez RL (1994) Metabolite signals regulate gene expression and source/sink relations in cereal seedlings. Plant Physiol 106:1235-1239   DOI
16 Muller B, Stosser M, Tardieu F (1998) Spatial distributions of tissue expansion and cell division rates are related to irradiance and to sugar content in the growing zone of maize roots. Plant Cell Environ 21:149-158   DOI
17 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497   DOI
18 Shin TY, Kim SH, Suk KH, Ha JH, Kim IK, Lee MG, Jun CD, Kim SY, Lim JP, Eun JS, Shin HY, Kim HM (2005) Anti-allergic effects of Lycopus lucidus on mast cell-mediated allergy model. Toxicol Appl Pharmacol 209:255-262   DOI
19 Xiong W, Chen GY, Tan DY, Zuo SY (2011) Hypoglycemic and hypolipidemic effect of polysaccharide from wild plant Lycopus lucidus Turcz. on alloxan-induced diabetic mice. Chin J Bioprocess Eng 9:45-48
20 Thorpe C, Yeung EC, de Klerk GJ, Roberts A, George EF (2007) Chapter 4. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems, p115-174. In: E.F. George MA Hall, GJ de Kler (eds). Plant propagation by tissue culture 3rd ed. Springer Dordrecht, NL
21 Xiong W, Jiao Y, Luo YH, Zhang CX, Tan DY, Zuo SY (2012). Effects of polysaccharide from Lycopus lucidus Turcz. on proliferation of tumor cells in vitro and in vivo. Chin J Bioprocess Eng 4:64-69
22 Yang X, Yan Z, Nianwu H, Kevin DC (2010) Isolation, Characterization, and Immunological Effects of $\alpha$-Galacto-oligosaccharides from a New Source, the Herb Lycopus lucidus Turcz. J Agric Food Chem 58: 8253-8258   DOI
23 Butcher DN, Street HE (1964) Excised root culture. Bot Rev 30:513-586   DOI
24 Yao Y, Yang J, Wang D, Zhou F, Cai X, Lu W, Hu C, Gu Z, Qian S, Guan X, Cao P (2013) The aqueous extract of Lycopus lucidus Turcz ameliorates streptozotocin-induced diabetic renal damage via inhibiting TGF-signaling pathway. Phytomedicine 20:1160-1167   DOI
25 Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837-2849   DOI
26 Yildiz M, Onde S, Ozgen M (2007) Sucrose effects on phenolic concentration and plant regeneration from sugarbeet leaf and petiole explants. J Sugar Beet Res 44:1-15   DOI
27 Yu JQ, Lei JC, Zhang XQ, Yu HD, Tian DZ, Liao ZX, Zou GL (2011) Anticancer, antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz. var. hirtus Regel. Food Chem 126:1593-1598   DOI
28 Zhou JG, Chen SY (2003) Tissue culture study of Lycopus lucidus. Journal of Henan Agricultural University 37:266-269
29 Ahmad T, Abbasi N, Hafiz IA, Ali A (2007) Comparison of sucrose and sorbitol as main carbon energy source in morphogenesis of peach rootstock GF-677. Pak J Bot 39:1264-1275
30 Baier M, Hemmann G, Holman R, Corke F, Card R, Smith C, Rook F, Bevan MW (2004) Characterization of mutants in Arabidopsis showing increased sugar-specific gene expression, growth, and developmental responses. Plant Physiol 134:81-91   DOI
31 Chen XJ, Zhang YQ (2010) Polysaccharide extract from Russula and its role of lowering blood glucose and lipid. Food Science 31:255-258