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http://dx.doi.org/10.5010/JPB.2002.29.2.105

Effect of DIF and Cytokinins on In Vitro Growth of 'Campbell Early' Crapes (Vitis spp.)  

Kim, Seung-Heui (Dept. of Horticulture, Chungbuk National University)
Kim, Seon-Kyu (Dept. of Horticulture, Chungbuk National University)
Publication Information
Journal of Plant Biotechnology / v.29, no.2, 2002 , pp. 105-110 More about this Journal
Abstract
Effects of difference between day and night temperatures (DIF) and cytokinins on in vitro growth of 'Campbell Early' grape plantlets were investigated, Shoot growth was suppressed by -7DIF (18/$25^{\circ}C$). Effects of BA and TDZ were not affected by DIF. 0DIF (25/$25^{\circ}C$) enhanced the growth with increasing cytokinin level. Kinetin at 10 $\mu$M was the most effective for shoot growth with 87 mm among cytokinins used except for control. Zeatin and kinetin each at 5 $\mu$M significantly increased shoot growth to respective 162 and 110 mm. In 0DIF, two-fold increase of 162 mm was obtained by 5 $\mu$M zeatin. In control, no further shoot proliferation was observed regardless of DIF. In 0 and ＋7DIF (25/18$^{\circ}C$), 4 shoots were observed with 10 $\mu$M BA. Zeatin, 2iP and kinetin resulted in poor shoot proliferation while BA at 10 $\mu$M resulted in profuse branching in all DIFs.
Keywords
BA; branching; kinetin; shoot growth; shoot proliferation; zeatin;
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