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http://dx.doi.org/10.5352/JLS.2020.30.5.428

Pupal Drone Extracts for Anti-wrinkle and Skin-lightening Materials  

Kim, Jung-Eun (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Kim, Do-Ik (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Koo, Hui-Yeon (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Kim, Hyeon-Jin (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Kim, Seong-Yeon (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Lee, Yoo-Beom (Jeon-nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute)
Moon, Jae-Hak (Department of Food Science & Technology, Cheon-nam National University)
Choi, Yong-Soo (Department of Agricultural Biology, National Institute of Agricultural Science, R.D.A.)
Publication Information
Journal of Life Science / v.30, no.5, 2020 , pp. 428-433 More about this Journal
Abstract
In this study, we created pupal stage extracts of Apis mellifera L. drones for use in cosmetic materials. The effect of the drone pupae extract (DPE) on HDF cells was assessed for analysis of anti-wrinkle activity by collagen or collagenase gene expression, and the skin-lightening effect was studied by in vitro tyrosinase inhibition and B16F10 melanoma assay; the two cells were found to be non-cellular when the concentration of DPE was 100 ㎍/ml. Albutin concentration (positive control) in the whitening test was set at a capacity of 100 ug/ml and m-melanocyte stimulating hormone (α-MSH). A melanin-producing induction material was set at a concentration of 100 nM, and the expression of collagen type I and MMP1 collagenase was measured using HDF cells. MMP1 expression was seen to reduce in a concentration-dependent manner in treatment with DPE. Inhibiting melanin generation with B16F12 cells indicated a tendency to decrease in the DPE treatment group. Both L-Tyrosine and L-DOPA as DPE were used in an in vitro tyrosinase induction test to demonstrate the effects of tyrosinase suppression on concentrations. The higher the concentration of DPE, the greater the wrinkle reduction and whitening effect. In conclusion, it was found that DPE is an effective smoothing and whitening material by increasing collagen generation and inhibiting collagenase expression and reducing melanin production.
Keywords
Anti-wrinkles; drone; drone pupa extract; honey bee; skin-lighting;
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Times Cited By KSCI : 4  (Citation Analysis)
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