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http://dx.doi.org/10.5352/JLS.2018.28.1.90

Characteristics of Eggshell Powder as Carriers of Probiotics  

Lee, Woo-Do (Department of Animal Science and Technology, Konkuk University)
Niu, Kai-Min (Department of Animal Science and Technology, Konkuk University)
Lim, Jeong-Min (Department of Animal Science and Technology, Konkuk University)
Yi, Kwon-Jung (Department of Animal Science and Technology, Konkuk University)
Lee, Bong-Joo (Aquafeed Research Center, National Institute of Fisheries Science)
Kim, Kang-Woong (Aquafeed Research Center, National Institute of Fisheries Science)
Kim, Kyoung-Duck (Aquafeed Research Center, National Institute of Fisheries Science)
Hur, Sang-Woo (Aquafeed Research Center, National Institute of Fisheries Science)
Han, Hyon-Sob (Aquafeed Research Center, National Institute of Fisheries Science)
Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
Publication Information
Journal of Life Science / v.28, no.1, 2018 , pp. 90-98 More about this Journal
Abstract
Eggshell (ES) is a by-product of table eggs with high content of calcium carbonate which can be used as a calcium source in feed. In this study, we have first illuminated the potential application of ES as a novel carrier for probiotics. The carriers used in the study include a SBM (Soybean meal), ESL (Eggshell powder with large particles), ESF (Eggshell powder with fine particles), and the complex carriers (SBM+ESL, SBM+ESF). The structure of carriers absorbed by L. plantarum was confirmed by SEM image. Among these carriers, the complex carrier SBM+ESF showed the highest viability of L. plantarum with pH 7~8 during four weeks storage at room temperature. The SBM+ESF was further tested as a carrier for various probiotic strains at $4^{\circ}C$ or $30^{\circ}C$. All the probiotic strains showed high viability at $4^{\circ}C$ storage. However, a significant reduction of Lactobacillus cells was observed at $30^{\circ}C$ storage. B. lichenifomis maintained high viability whereas B. subtilis, B. amyloliquefaciens, and S. cerevisiae showed the reduction of $2{\log}_{10}$ (CFU/g). These results suggest that if the ESF as a calcium source in feed was mixed with SBM, it can be used as an effective complex carrier for improving the viability of some probiotics including B. licheniformis.
Keywords
Carrier; eggshell powder; probiotics; SEM; viability;
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