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http://dx.doi.org/10.5352/JLS.2017.27.4.450

Comparison of Anti-asthmatic Activity by Native Codonopsis lanceolata Extract  

Lee, Seung-Ha (Departments of Food Industrial Engineering, Graduate School, Kyungpook National University)
Choi, Hee-Jeong (Departments of Food Science and Biotechnology, Graduate School, Kyungpook National University)
Heo, Jin-Chul (Departments of Biomedical Engineering, School of Medicine, Keimyung University)
Lee, Jong-Ha (Departments of Biomedical Engineering, School of Medicine, Keimyung University)
Kwon, Taeg Kyu (Departments of Immunology, School of Medicine, Keimyung University)
Ha, Sang-Chul (Department of Hotel Culinary Arts, Daegu Mirae College)
Lee, Sang-Han (Departments of Food Industrial Engineering, Graduate School, Kyungpook National University)
Publication Information
Journal of Life Science / v.27, no.4, 2017 , pp. 450-455 More about this Journal
Abstract
Codonopsis lanceolata (Campanulaceae) has been widely used in traditional medicine and is considered to have medicinal properties to treat diseases and symptoms such as bronchitis, coughs, spasm, edema, hepatitis, colitis, and lung injury. In order to investigate whether native Codonopsis lanceolata extract alleviates ashmatic symptoms in vivo, we first carried out various antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC) assays. The antioxidant activities were increased by adding Codonopsis lanceolata extract in a concentration-dependent manner which compared to ascorbic acid as a positive control. Histological studies using an ovalbumin-induced animal model exhibited potent anti-inflammatory potential by decreasing immuno-responsive cells in the lung by the extract by confirming H&E and PAS staining. It is revelaed that further immunihistochemical analysis showed anti-ashmatic capabilities by assessing histamine, IL-31, and MMP-9 expressions. The level of IL-13 expression in Codonopsis lanceolata extract-treated group was decreased upto 73.7% compared to control, whereas that of total cells and eosinophil counting in Codonopsis lanceolata extract-treated group was diminished to 73.5% and 80.9%, respectively. These results collectively indicate that the C. lanceolata extract ameliorates asthmatic symptoms effectively in an ovalbumin-challenged mice model, in that the extract can be used for the development of an anti-asthmatic food ingredient.
Keywords
Anti-oxidant; asthma; Codonopsis lanceolata; immune response; inflammation;
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