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http://dx.doi.org/10.5352/JLS.2017.27.4.398

Cloning and Characterization of BTG-1 Gene from Pacific Oyster (Crassostrea gigas)  

Chung, In Young (Department of Microbiology, Pukyong National University)
Oh, Jeong Hwan (Institute of Fisheries Science, College of Fisheries Science, Pukyong National University)
Song, Young Hwan (Department of Microbiology, Pukyong National University)
Publication Information
Journal of Life Science / v.27, no.4, 2017 , pp. 398-407 More about this Journal
Abstract
BTG 1 (B-cell translocation gene 1) gene was first identified as a translocation gene in a case of B-cell chronic lympocytic leukemia. BTG1 is a member of the BTG/TOB family with sharing a conserved N-terminal region, which shows anti-proliferation properties and is able to stimulate cell differentiation. In this study, we identified and characterized the pacific oyster Crassostrea gigas BTG1 (cg-BTG1) gene from the gill cDNA library by an Expressed Sequence Tag (EST) analysis and its nucleotide sequence was determined. The cg-BTG1 gene encodes a predicted protein of 182 amino acids with 57% 56% identities to its zebrafish and human counterparts, and is an intron-less gene, which was confirmed by PCR analysis of genomic DNA. Maximal homologies were shown in conserved Box A and B. The deduced amino acid sequence shares high identity with other BTG1 genes of human, rat, mouse and zebrafish. The phylogenic analysis and sequence comparison of cg-BTG1 with other BTG1 were found to be closely related to the BTG1 gene structure. In addition, the predicted promoter region and the different transcription-factor binding site like an activator protein-1 (AP-1) response element involved in negative regulation and serum response element (SRE) were able to be identified by the genomic DNA walking experiment. The quantitative real-time PCR analysis showed that the mRNA of cg-BTG1 gene was expressed in gill, heart, digestive gland, intestine, stomach and mantle. The cg-BTG1 gene was expressed mainly in heart and mantle.
Keywords
Abundant in neuroepithelium area; B-cell translocation gene; Crassostrea gigas; PMRT1; protein arginine methyltransferase 1;
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