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http://dx.doi.org/10.5352/JLS.2011.21.9.1266

Effect of Saponin with Antioxidant Activity on Matrix Metalloproteinase in Human Dermal Fibroblasts  

Park, Hye-Jung (Department of Chemistry, Dong-Eui University)
Kim, Moon-Moo (Department of Chemistry, Dong-Eui University)
Lee, Dong-Hwan (Department of Chemistry, Dong-Eui University)
Publication Information
Journal of Life Science / v.21, no.9, 2011 , pp. 1266-1273 More about this Journal
Abstract
Saponin is a main component of ginseng widely known as an oriental traditional medicinal ingredient. A variety of biological effects of saponin has been reported, but its action related to skin regeneration has remained unclear so far. In this study, the effect of saponin on matrix metalloproteinase as well as its antioxidant effect in cell free system was examined in human dermal fibroblasts. First of all, as a result of investigating the effect of saponin on cell viability using MTT assay, it was shown to increase cell viability below 10 ${\mu}g$/ml, but it also showed cytotoxicity above 25 ${\mu}g$/ml. The antioxidant effect of saponin was exerted by inhibition of $H_2O_2$ in addition to reducing power above 1 ${\mu}g$/ml. In particular, saponin showed a protective effect on DNA oxidation. Furthermore, it was observed that saponin activates MMP-2 and increases MMP-1 activity in gelatin and casein zymography analyses, respectively, indicating that saponin could have potential a therapeutic agent for anti-aging and skin regeneration.
Keywords
Saponin; skin regeneration; human dermal fibroblasts; ROS; matrix metalloproteinase;
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1 Simon, H., A. Haj-Yehia, and F. Levi-Schaffer. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 5, 415-418.   DOI
2 Sounni, N., M. Janssen, J. Foidart, and A. Noel. 2003. Membrane type-1 matrix metalloproteinase and TIMP-2 in tumor angiogenesis. Matrix Biol. 22, 55-61.   DOI
3 Stadtman, E. and C. Oliver. 1991. Metal-catalyzed oxidation of proteins. Physiological consequences. J. Biol. Chem. 266, 2005-2008.
4 Vihinen, P. and V. Kahari. 2002. Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets. Int. J.Cancer 99, 157-166.   DOI
5 Wang, C., D. Shi, and H. Yin. 2007. Effect of panax quinquefolius saponin on angiogenesis and expressions of VEGF and bFGF in myocardium of rats with acute myocardial infarction]. Zhongguo Zhong Yi Yan Jiu Yuan Zhu Ban. 27, 331-334.
6 Yoshiki, Y. and K. Okubo. 1995. Active oxygen scavenging activity of DDMP (2, 3-dihydro-2, 5-dihydroxy-6-methyl-4H-pyran-4-one) saponin in soybean seed. Biosci. Biotechnol. Biochem. 59, 1556-1557.   DOI
7 Zhao, H. and H. Yang. 2008. Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Sci. Hortic. 116, 442-447.   DOI
8 Kang, J. H., I. H. Han, M. K. Sung, H. Yoo, Y. G. Kim, J. S. Kim, T. Kawada, and R. Yu. 2008. Soybean saponin inhibits tumor cell metastasis by modulating expressions of MMP-2, MMP-9 and TIMP-2. Cancer Lett. 261, 84-92.   DOI
9 Kang, J., M. Sung, T. Kawada, H. Yoo, Y. Kim, J. Kim, and R. Yu. 2005. Soybean saponins suppress the release of proinflammatory mediators by LPS-stimulated peritoneal macrophages. Cancer Lett. 230, 219-227.   DOI
10 Kang, S., S. Cho, J. Chung, C. Hammerberg, G. Fisher, and J. Voorhees. 2005. Inflammation and extracellular matrix degradation mediated by activated transcription factors nuclear factor-{kappa} B and activator protein-1 in inflammatory acne lesions in vivo. Am. J. Pathol. 166, 1691-1699.   DOI
11 Kim, H. G., I. S. Oh, S. S. So, and C. W. Park. 2001. Effect of VEGF on the secretion of MMP-2 and plasmin from human Keratinocytes cells. Korean J. Biotechnol. Bioeng. 16, 237-240.
12 Neufeld, G., T. Cohen, S. Gengrinovitch, and Z. Poltorak. 1999. Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 13, 9-22.
13 Lee, K., S. Hwang, J. Choi, and H. Jeong. 2008. Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF-[kappa] B activation via ROS signal pathway. Cancer Lett. 268, 233-243.   DOI
14 Maniatis, T. 1989. Molecular cloning: a laboratory manual/J. Sambrook, EF Fritsch, T. Maniatis: New York: Cold Spring Harbor Laboratory Press.
15 Milne, L., P. Nicotera, S. Orrenius, and M. Burkitt. 1993. Effects of glutathione and chelating agents on copper-mediated DNA oxidation: pro-oxidant and antioxidant properties of glutathione. Arch. Biochem. Biophys. 304, 102-109.   DOI
16 Oyaizu, M. 1986. Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Jpn. J. Nutr. 44, 307-315.   DOI
17 Choi, C. W., S. C. Kim, S. S. Hwang, B. K. Choi, H. J. Ahn, M. Y. Lee, S. H. Park, and S. K. Kim. 2002. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci. 163, 1161-1168.   DOI
18 Cho, S. J., H. J. Baik., S. S. Lee, I. M. Chung, J. H. Ha, J. S. Kang, H. C Koh,I. C Shin, and C. H. Lee. 2000. Studies on the ROS (Reactive Oxygen Species)-scavenging activities of DDMP saponin isolated from glycine mac (L.) Merrill. J. Appl. Pharmacol. 8, 32-37.
19 Choi, S. 2002. Epidermis proliferative effect of the Panax ginseng ginsenoside Rb 2. Arch. Pharm. Res. 25, 71-76.   DOI
20 Fath, A., P. Bethke, and R. Jones. 2001. Enzymes that scavenge reactive oxygen species are down-regulated prior to gibberellic acid-induced programmed cell death in barley aleurone. Plant Physiol. 126, 156-166.   DOI
21 Fukuda, N., H. Tanaka, and Y. Shoyama. 2000. Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography. J. Nat. Prod. 63, 283-285.   DOI
22 Hansen, M. B., S. E. Nielsen, and K. Berg. 1989. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods 119, 203-210.   DOI
23 Hofmann, U. B., J. R. Westphal, G. N. P. van Muijen, and D. J. Ruiter. 2000. Matrix metalloproteinases in human melanoma. J. Invest. Dermatol. 115, 337-344.   DOI
24 Hollborn, M., C. Stathopoulos, A. Steffen, P. Wiedemann, L. Kohen, and A. Bringmann. 2007. Positive feedback regulation between MMP-9 and VEGF in human RPE cells. Invest. Ophth. Vis. Sci. 48, 4360-4367.   DOI
25 Imai, J., N. Ide, S. Nagae, T. Moriguchi, H. Matsuura, and Y. Itakura. 1994. Antioxidant and Radical Scavanging Effects of Aged Garlic Extract and its Constituents. Planta Med. 60, 417-417.   DOI
26 Beckman, K. B. and B. N. Ames. 1998. The free radical theory of aging matures. Physiol. Rev. 78, 547-581.
27 Ali, M. B., K. W. Yu, E. J. Hahn, and K. Y. Paek. 2005. Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng CA Mayer and Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. Plant Sci. 169, 83-92.   DOI
28 Alscher, R., N. Erturk, and L. Heath. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot. 53, 1331-1341.   DOI
29 Bandyopadhyay, U., D. Das, and R. Banerjee. 1999. Reactive oxygen species: Oxidative damage and pathogenesis. Curr Sci. 77, 658-666.
30 Bogenrieder, T. and M. Herlyn. 2003. Axis of evil: molecular mechanisms of cancer metastasis. Oncogene 22, 6524-6536.   DOI