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http://dx.doi.org/10.5352/JLS.2009.19.4.549

Analysis of Trans-splicing Transcripts in Embryonic Stem Cell  

Ha, Hong-Seok (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Huh, Jae-Won (National Primate Research Center, KRIBB)
Kim, Dae-Soo (Korean BioInformation Center, KRIBB)
Park, Sang-Je (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Bae, Jin-Han (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Ahn, Kung (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Yun, Se-Eun (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Kim, Heui-Soo (Department of Biological Sciences, College of Natural Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.19, no.4, 2009 , pp. 549-552 More about this Journal
Abstract
Genetic mutations by gene fusion result from chromosomal rearrangement, trans-splicing, and intergenic splicing. Trans-splicing is a phenomenon in which two pre-mRNAs grow together into one. We analyzed the trans-splicing products in embryonic stem cells. By using bioinformatic tools, 70 trans-splicing transcripts were identified. They are classified into 6 types according to fusion pattern: 5'UTR-5'UTR, 5'UTR-3'UTR, 3'UTR-3'UTR, 5'UTR-CDS, 3'UTR-CDS, CDS-CDS. The fusion products are more abundant in CDS regions than in UTR regions, which contain multiple intron numbers. Chromosome analysis showing gene fusion via trans-splicing indicated that chromosomes 17 and 19 were activated. These data are of great use for further studies in relation to fusion genes and human diseases.
Keywords
Fusion gene; trans-splicing; bioinformatics; genetic mutation; chromosome analysis;
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